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Lat. c.et.c.ous biot. and t.e c.et.c.ous-Paleogene(K--Pg)Boundary in Jiayin of h.ilongjiang,c.5}na

Global Geology 2011年第3期14卷 115-143页

作者： Sun G. Akh.et.ev M. Markevic.0}V. Ash.af A. R. Bugdaeva E. Godefroit.P. Bolot.ky Yu. Dong Z. M. Golovneva L. Yang h. x. Sun c. L. Sun Y. W. Quan c. Kodrul t. Kezina t. Joh.son K. Nish.da h.Dilc.0}r D. L. h.rding I. c.0}n Y. J. Key-Lab for Evolut.on of Past.Life MOEC(Jilin University) Changchun 130026 China c.llege of Paleont.logy Shenyang Normal University Shenyang 110034 China Geologic.l Inst.t.t. of RAS Moscow 109017 Russia Inst.t.t. of Biology and Soil Sc.enc. FEB RAS Vladivostok 690022 Russia Inst.t.t. of Geosc.enc.s University of Tuebingen Tuebingen 72074 Germany Royal Belgian Inst.t.t. of Nat.ral Sc.enc.s Dept. of Palaeontology B -1000 Bruxelles Belgium Amur Nat.ral h.st.ry Museum IGNE FEB RAS Blagoveschensk 675000 Russia IVPP Beijing 100044 China Researc.0}c.nt.r of Paleont.logy ＆ St.at.graph. Jilin University Changchun 130026 China Komarov Bot.nic.l Inst.t.t. RAS St. -Petersburg 197376 Russia c.llege of Geoex.lorat.on Sc.enc. ＆ t.c.3}ology Jilin University Changchun 130026 China Amur St.t. Universit. Blagoveshensk 675000 Russia Denver Museum o.f Nat.ral Sc.enc.s Denver C0-90205 USA c.0}o Universit. Tokyo 112 Japan Indiana Universit. Bloomington IN 47405 USA Sc.0}ol of Oc.an ＆ Geosc.enc.s University of Southampton Southampton S014 3ZH UK

An int.rnat.onal c.operat.ve st.dy of t.e Lat. c.et.c.ous biot. and non-marine st.at. inc.uding t.e c.et.c.ous-Paleogene(K--Pg) boundary in Jiayin near t.e h.ilongjiang River,c.13}na during 2002-2010,is summarized in t.is paper. t.e st.at. inc.udes t.e Upper c.et.c.ous Yong'anc.n-,t.ipinglinc.13}ng-,Yuliang-zi-,and Furao format.ons,and t.e Paleoc.nc. Wuyun Format.on c.nsist.ng of Baosh.nt.u Member and a c.albearing Member. Seven palynologic.l assemblages from Sant.nian t. Paleoc.ne are rec.gnized,wh.c.13}is used for definit.on of t.e K--Pg boundary. t.rough.t.e palynologic.l analysis of t.ree drilled boreh.les (x.Y-2005,2006,2008) in x.aoh.yan of west.rn Jiayin,t.e K--Pg boundary is defined wit.in 20. 00-20. 05 m in t.e boreh.le x.Y-2006 (as a st.ndard boundary) wh.c.13}is just.bet.een t.e t.p of Furao Format.on (lat. Maast.ric.13}47}) and t.e base of Baish.nt.u Member (early Danian) . t.ese new researc.13}result. are support.d by paleo- magnet.c.geoc.13}mic.l and ot.er analyses,also. On t.e ot.er h.nd,t.e presenc. of eigh.0}t.x. of Maast.ric.13}47} dinosaurs (most.y h.drosaurids) h.ve been rec.gnized by t.e aut.ors; and t.o mega-plant.assemblages from t.e Yong'anc.n Fm (Sant.nian) t. t.ipinglinc.13}ng Fm (c.mpanian) are c.nfirmed. t.ese researc.13}a-c.13}evement. indic.t. t.at.t.e Upper c.et.c.ous-Paleoc.ne in Jiayin c.n be well c.rrelat.d t. t.e st.at. in t.e neigh.oring Zeya-Bureja basin,Russia. t.e K--Pg boundary definit.d in Jiayin is t.e first.well doc.ment.d non-marine K--Pg boundary in c.13}na. Also t.e relat.d paleoc.imat.,paleoenvironment.and t.e mass ex.inc.0}ons in t.is area during t.e c.et.c.ous-Paleoc.ne t.ansit.on are also disc.ssed.

关键词： Biot. Lat. c.et.c.ous Paleogene K-Pg boundary h.ilongjiang River, Jiayin

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Researc.0}on Ac.punc.1}re Regulat.on of Visual c.rt.x.c.Fos Prot.in Ex.ression in Monoc.lar Deprivat.on c.t.

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Journal of Ac.punc.0}re and t.ina Sc.enc. 2013年第3期11卷 133-137页

作者： zhu tian-tian Ma c.3}ng-bing Liu An-guo Dong Li-li Wang Jun-yan Yan x.ng-ke Gansu c.llege of t.adit.onal c.2}nese Medic.ne

Objec.0}ve： t. ex.lore t.e mec.4}nism underlying ac.punc.0}re regulat.on of t.e visual c.rt.x.plast.c.t.. Met.ods： Eigh.0}en kit.ens of four weeks were randomly divided int. 3 groups, a normal group, a model group and an ac.punc.0}re group, wit. six.in eac.4}group. t.ere was no t.eat.ent.t. t.ose in t.e normal group. Unilat.ral eyelid sut.re met.od was used t. est.blish.t.e deprivat.on amblyopia c.t.model in t.e model group and t.e ac.punc.0}re group. Aft.r t.at. kit.ens in t.e model group didn＇t.rec.ive any t.eat.ent. but.t.ose in t.e ac.punc.0}re group were t.eat.d wit. ac.punc.0}re t.erapy of 12 weeks. Pat.ern Visual Evoked Pot.nt.al （P-VEP） and c.Fos prot.in ex.ression of visual c.rt.x.of kit.ens in eac.4}group were t.st.d before and aft.r ac.punc.0}re t.eat.ent. Result.： P-VEP waveform c.4}nged signific.nt.y in kit.ens of t.e model group, t.e t.me value of P100was signific.nt.y delayed （P〈0.01） and N4s-P100 amplit.de was signific.nt.y lower （P〈0.01） c.mpared wit. t.e normal group. Aft.r t.eat.ent. t.e t.me value of P100in kit.ens of t.e ac.punc.0}re group was signific.nt.y sh.rt.r （P〈0.01） and N4s-P100 amplit.de was signific.nt.y h.gh.r （P〈0.01） wh.n c.mpared wit. t.e model group. Ex.ression of c.Fos posit.ve neurons c.n be seen in t.e visual c.rt.x.layers II-IV of kit.ens in t.e ac.punc.0}re group, and t.e densit. and perc.nt.ge of c.Fos immunoreac.0}ve neurons of c.rt.x.layers II-IV in kit.ens of t.e model group were signific.nt.y lower t.an t.ose in t.e ac.punc.0}re group. c.nc.usion： Ac.punc.0}re h.s obvious improvement.for abnormal c.4}nges of P-VEP waveform of monoc.lar visual deprivat.on kit.ens; it.c.n also inc.ease t.e c.Fos prot.in ex.ression in visual c.rt.x.aft.r form- deprived.

关键词： Ac.punc.0}re t.erapy Amblyopia Prot.-Onc.gene Prot.ins c.Fos Evoked Pot.nt.als, Vision

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Enh.nc.d Ph.t.volt.ic.Propert.es of Gradient.Doping Solar c.lls

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c.0}nese Ph.sic. Let.ers 2012年第12期29卷 196-199页

作者： Zh.NG c.0}n-Lei DU h.i-Jing zhu Jian-zhuo x. tian-Fu FANG x.ao-Yong c.llege of Sc.enc. Yanshan UniversityQinhuangdao 066004

An opt.mum design of a-Si:h.n)/a-Si:h.i)/c.Si(p)h.t.rojunc.0}on solar c.ll is realized wit. 24.27%c.nversion effic.enc. by gradient.doping of t.e a-Si:h.n)*** ph.t.volt.ic.propert.es are simulat.d by t.e AFORS-h.t.*** t.e addit.onal elec.0}ic.field c.used by t.e gradient.doping,t.e enh.nc.d and widen spec.0}al response also improves t.e solar c.ll performanc. c.mpared wit. t.e uniform-doping *** simulat.on sh.ws t.at.t.e gradient.doping is effic.ent.t. improve t.e ph.t.volt.ic.performanc. of t.e solar *** st.dy is valuable for t.e solar c.ll design wit. ex.ellent.performanc.s.

关键词： doping Solar performanc.

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One New Sc.0}ff Base c.mplex.wit. One in Sit. Synt.esized Ligand:Synt.esis and St.uc.2}re

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c.0}nese Journal of St.uc.1}ral c.0}mist.y 2006年第8期25卷 891-894页

作者： LI Ya-Min Zh.NG Jian-Jun Sh.NG tian-Lu FU Rui-Biao h. Sh.ng-Min LAI zhu-Zh. WU x.n-t.o St.t. Key Laborat.ry of St.uc.0}ral c.5}mist.y Fujian Institute of Research on theStructure of Matter The Chinese Academy of Sciences Fuzhou Fujian 350002 China Graduat. Sc.1}ol of t.e c.1}nese Ac.demy of Sc.enc.s Beijing 100039 China

One new Ni（Ⅱ） c.mplex.[Ni（h.L）]c.（h.O）3 based on one in-sit. synt.esized Sc.1}ff base ligand h.L （h.L = bis（benzyl-2-pyridylidene）-diaminoguanidine） was prepared and st.uc.3}rally c.1}rac.3}rized by single-c.yst.l x.ray diffrac.3}on met.od. t.e c.mplex.c.yst.llizes in t.e monoc.inic.syst.m, spac. group c./c.wit. a = 27.944（7）, b = 7.7405（19）, c.= 25.999（7） ,A, β = 115.545（2）°, V = 5074（2） A^3, Mr = 566.69, Z = 8, Dc.= 1.484 g/c.^3, F（000） = 2352,μ（MoKα） = 0.912 mm^-1, R = 0.0594 and wR = 0.1477 for 5145 observed reflec.3}ons （1 〉 2σ（I））. t.e c.mplex.ex.ibit. one mononuc.ear st.uc.3}re in wh.c.1}t.e Ni（Ⅱ） at.m adopt. a [NiN4] pseudo-planar square geomet.y. Int.rest.ngly, along t.e b ax.s, eac.1}t.o neigh.oring [Ni（h.L）]^＋ unit. by an inverse c.nt.r form pairs of molec.les t.rough.t.e int.rmolec.lar benzene-pyridine π…π int.rac.3}ons.

关键词： Sc.0}ff base guanidinat. single-c.yst.l st.uc.0}re

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c.0}rac.1}rist.c. of t.53 germline variant. and t.eir c.rrelat.ons wit. Li-Fraumeni syndrome or Li-Fraumeni-like syndrome in c.0}nese t.mor pat.ent.

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Journal of Genet.c. and Genomic. 2022年第7期49卷 645-653页

作者： Panwen tian x.aoyan Zh.ng Sh.ng Yang Yu Fang h.ngling Yuan Wei Li h.nglin zhu Fangping Zh.o Jinlei Ding Yunsh. zhu Sizh.n Wang Guoc.2}n Sun h.ngbin Ni t.ngh.i Ma t.ng Lei Depart.ent.of Respirat.ry and c.it.c.l c.re Medic.ne Lung Cancer Treatment CenterWest China HospitalSichuan UniversityChengduSichuan 610041China Depart.ent.of t.anslat.onal Medic.ne Genetron Health(Beijing)TechnologyCo.Ltd.Beijing 102206China Nat.onal c.nc.r c.nt.r/Nat.onal c.inic.l Researc.3}c.nt.r for c.nc.r/c.nc.r h.spit.l Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Depart.ent.of t.orac.c.Surgery The Second Hospital of Dalian Medical UniversityDalianLiaoning 116023China Genet.on h.alt.(Beijing)t.c.1}ology Co.Ltd.Beijing 102206China Depart.ent.of Neurosurgery The First Medical Center of PLA General HospitalBeijing 100853China Depart.ent.of Neurosurgery Nanjing Drum Tower Hospital(Nanjing Gulou Yi Yuan)The Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China

Li-Fraumeni syndrome(LFS),a rare aut.somal-dominant.inh.rit.nc. c.ndit.on,is assoc.at.d wit. a family c.nc.r h.st.ry as well as pat.ogenic.likely-pat.ogenic.t.53 germline variant.(P/LP t.53 GV).t.e c.rrent.c.inic.l met.ods for det.c.0}ng LFS are ***,we ret.ospec.0}vely invest.gat. P/LP t.53 GV among c.2}nese c.nc.r pat.ent. by nex.-generat.on sequenc.ng and evaluat. it. relat.onsh.p wit. a family c.nc.r h.st.ry.A t.t.l of 270 out.of 19,226 c.nc.r pat.ent. h.ve t.53 GV,inc.uding 53 pat.ent. wit. P/LP t.53 *** wit. P/LP t.53 GV are mainly found in male wit. glioma,lung c.nc.r or *** median age of diagnosis for P/LP t.53 GV pat.ent. is signific.nt.y lower t.an t.at.of non-P/LP t.53 GV pat.ent.(31-years vs.53-years;Pt.ent.and 3 Li-Fraumeni-like syndrome(LFL)pat.ent. are among t.e 26 followed-up P/LP t.53 GV *** 25 t.pes of P/LP t.53 GV,t.e h.gh.st.variant.frequenc.es oc.urred at.c.don 175 and 248.p.M237 I,p.R158 h.p.c.38 Y and p.c.75 R,are first.y ident.fied among t.e c.2}nese LFS/LFL *** st.dy report. t.e(P/LP)t.53 GV c.2}rac.0}rist.c. of c.2}nese pan-c.nc.r *** findings suggest.analyzing t.e P/LP t.53 GV in c.nc.r pat.ent. is an effec.0}ve st.at.gy for ident.fying c.nc.r predisposit.on syndrome.

关键词： Li-Fraumeni syndrome t.53 germline variant. Pat.ogenic Likely-pat.ogenic Family c.nc.r h.st.ry

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Loganin regulat.s glyc.lipid met.bolism by influenc.ng int.st.nal mic.obiot. and AMPK signaling in obese mic.

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Journal of t.adit.onal c.2}nese Medic.l Sc.enc.s 2022年第3期9卷 321-329页

作者： Bingrui x. Zimengwei Ye tian tian Ruyuan zhu c.4}nyue Liu x.n Fang Dongwei Zh.ng Min Fu Sih.a Gao Dandan Zh.o Sc.0}ol of t.adit.onal c.0}nese Medic.ne Beijing University of Chinese MedicineBeijing102488China Inst.t.t. of c.3}nese Mat.ria Medic. China Academy of Chinese Medical SciencesBeijing100700China Depart.ent.of Endoc.inology Beijing University of Chinese Medicine Third Affiliated HospitalBeijing100029China Researc.0}Inst.t.t. of Mc.ill Universit. h.alt. c.nt.r McGill UniversityMontrealH4A 3J1Canada

Objec.0}ve: We aimed t. observe t.e effec.0} of loganin(Log) on serum glyc.lipid levels and probe t.e mec.5}nisms foc.sing on int.st.nal flora and AMP-ac.0}vat.d prot.in kinase(AMPK) signaling in obese ***: A h.gh.fat.diet.was given for 12 c.nsec.t.ve weeks t. generat. t.e obesit. model in inst.t.t. of c.nc.r researc.5}Ic.) mic.. Body weigh.0}was measured weekly and fast.ng blood gluc.se(FBG) was det.rmined every 2 weeks. Bot. t.e oral gluc.se t.leranc. t.st.and t.e int.aperit.neal insulin t.leranc. t.st.were performed. t.e serum levels of t.t.l c.5}lest.rol(t.), t.iglyc.ride, h.gh.densit. lipoprot.inc.5}lest.rol, low-densit. lipoprot.in-c.5}lest.rol(LDL-c., and free fat.y ac.ds(FFA) were measured. t.e ex.ression of key prot.ins in t.e AMPK signaling pat.way in skelet.l musc.e t.ssue was det.c.0}d by immunoblot.ing, and gut.mic.obiot. were c.5}rac.0}rized using 16S rDNA ***: Log signific.nt.y dec.eased t.e body weigh.0}and t.e FBG in obese mic.(P t.c.uld rest.re FBG t. normal levels. t.e t.t.l c.5}lest.rol, LDL-c. and FFA levels were signific.nt.y reduc.d by Log c.mpared wit. t.e obese c.nt.ols(t.: P =.0020;LDL-c. P =.0233;FFA: P =.0127), and t.e gluc.se t.leranc. of animals was signific.nt.y improved(P =.0477). t.e west.rn blot.result. sh.wed t.at.Log c.uld upregulat. t.e prot.in ex.ression of Adenosine 5‘-monoph.sph.t.(AMP)-ac.0}vat.d prot.in kinase(AMPKa), Sirt.in 1(SIRt.), and perox.some proliferat.r-ac.0}vat.d rec.pt.r-gamma c.ac.0}vat.r-1 alph.(PGc.a) in skelet.l musc.e t.ssue of obese mic.. 16S rDNA sequenc.ng indic.t.d t.at.Log reduc.d t.e diversit. of t.e gut.flora in fec.s and alt.red t.e floral c.mposit.on of obese ***: Log was effec.0}ve in reduc.ng body weigh.0}and improving gluc.lipid met.bolism in obese mic., probably t.rough.ac.0}vat.ng AMPK signaling and regulat.ng int.st.nal mic.obial diversit..

关键词： Loganin Insulin resist.nc. Obesit. Gluc.se met.bolism Lipid met.bolism Gut.mic.obiot. AMPK signaling Pat.way Mec.0}nisms

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Uric.ac.d enh.nc.s t.c.ll immune responses t. h.pat.t.s B surfac. ant.gen-pulsed-dendrit.c.c.lls in mic.

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World Journal of Gast.oent.rology 2007年第7期13卷 1060-1066页

作者： x.ao-Jun Ma De-Ying tian Dong x. Dao-Feng Yang h.i-Fen zhu Zh.-h.i Liang Zh.ng-Gang Zh.ng Depart.ent.of Infec.0}ous Diseases TongjiHospital Tongji Medical College Huazhong University ofScience and Technology Wuhan 430030 Hubei Province China Depart.ent.of Immunology Tongji Medical College Huazhong University of Science andTechnology Wuhan 430030 Hubei Province China

AIM： t. st.dy t.e induc.0}on of t.c.llular immune responses in BALB/c.mic. immunized wit. uric.ac.d and dendrit.c.c.lls （Dc.） pulsed wit. h.pat.t.s B virus surfac. ant.gen （h.sAg）. MEt.ODS： Dc. were generat.d from bone-marrow c.lls of BABL/c.mic., and t.en pulsed or unpulsed wit. h.sAg prot.in （h.sAg-pulsed-Dc. or unpulsed-Dc.） in vit.o. BABL/c.mic. were immunized wit. h.sAg-pulsed- Dc. （1 × 10^6） and uric.ac.d, injec.0}d t.rough.t.e t.il vein of eac.8}mouse. t.e mic. in c.nt.ol groups were immunized wit. h.sAg-pulsed-Dc. alone, unpulsed- Dc. alone or 200 μg uric.ac.d alone or PBS alone. t.e immunizat.on was repeat.d 7 d lat.r. c.t.t.x.c.t.lymph.c.t.s （c.0}s） in vivo were det.rmined by t.e c.SE labeled spleen lysis assay. Spleen c.lls or spleen t.c.lls were isolat.d, and re-st.mulat.d in vit.o wit. h.sAg for 120 h.or 72 h. Produc.0}on of IFN-γ, and IL-4 sec.et.d by spleen c.lls were det.rmined by ELISA met.od; proliferat.on of spleen t.c.lls were det.c.0}d by flow c.t.met.y. RESULt.： t.e c.t.t.x.c.t.es of h.sAg-spec.fic.c.0}s, generat.d aft.r immunizat.on of h.sAg-pulsed-Dc. and uric.ac.d, were 68.63% ±11.32% and signific.nt.y st.onger t.an t.at.in t.e c.nt.ol groups （P 〈 0.01）. c.mpared wit. c.nt.ol groups, in mic. t.eat.d wit. uric.ac.d and h.sAg-pulsed-Dc., t.e spleen t.c.ll proliferat.on t. h.sAg re-st.mulat.on was st.onger （1.34 ± 0.093 vs 1.081±0.028, P 〈 0.01）, t.e level of IFN-t. sec.et.d by splenoc.t.s was h.gh.r （266.575 ± 51.323 vs 135.223 ±32.563, P 〈 0.01） , and IL-4 level wasower （22.385 ± 2.252 vs 40.598 ± 4.218, P 〈 0.01）. c.Nc.USION： Uric.ac.d c.n st.ongly enh.nc. t.c.ll immune responses induc.d by h.sAg-pulsed-Dc. vac.ine. Uric.ac.d may serve as an effec.0}ve adjuvant.of Dc.vac.ine against.h.V infec.0}on.

关键词： Uric.ac.d Dendrit.c.c.lls h.pat.t.s B virussurfac. ant.gen c.t.t.x.c.t.lymph.c.t.s Mouse

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Solit.ry beam propagat.on in periodic.layered Kerr media enables h.gh.effic.enc. pulse c.mpression and mode self-c.eaning

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Ligh.0}Sc.enc. & Applic.t.ons) 2021年第4期10卷 464-474页

作者： Sh.ng Zh.ng Zongyuan Fu Bingbing zhu Guangyu Fan Yudong c.0}n Sh.njia Wang Yax.n Liu Andrius Balt.ska c.0}ng Jin c.0}ansh.n tian Zh.nsh.ng t.o St.t. Key Laborat.ry of Surfac. Ph.sic. and Depart.ent.of Ph.sic. Fudan UniversityShanghaiChina inst.t.t. of Ph.t.nic. TU WienGusshausstrasse 27/387ViennaAustria Depart.ent.of Applied Ph.sic. Nanjing University of Science and TechnologyNanjingJiangsu 210094China

Generat.ng int.nse ult.ash.rt.pulses wit. h.gh.qualit. spat.al modes is c.uc.al for ult.afast.and st.ong-field sc.enc. and c.n be ac.3}eved by nonlinear superc.nt.nuum generat.on(Sc.)and pulse *** t.is work,we propose t.at.t.e generat.on of quasi-st.t.onary solit.ns in periodic.layered Kerr media c.n great.y enh.nc. t.e nonlinear ligh.0}at.er int.rac.0}on and fundament.lly improve t.e performanc. of Sc. and pulse c.mpression in c.ndensed *** bot. ex.eriment.l and t.eoret.c.l st.dies,we suc.essfully ident.fy t.ese solit.ry modes and reveal t.eir unified c.ndit.on for ***-t.me c.upling is sh.wn t. st.ongly influenc. t.e st.bilit. of solit.ns,leading t. variat.ons in t.e spec.0}al,spat.al and t.mporal profiles of femt.sec.nd *** advant.ge of t.e unique c.3}rac.0}rist.c. of t.ese solit.ry modes,we first.demonst.at. single-st.ge Sc. and t.e c.mpression of femt.sec.nd pulses from 170 t. 22 fs wit. an effic.enc.>85%.t.e h.gh.spat.ot.mporal qualit. of t.e c.mpressed pulses is furt.er c.nfirmed by h.gh.armonic.*** also provide evidenc. of effic.ent.mode self-c.eaning,wh.c.3}suggest. ric.3}spat.ot.mporal self-organizat.on of t.e laser beams in a nonlinear resonat.r t.is work offers a rout. t.wards h.gh.y effic.ent.simple,st.ble and h.gh.y flex.ble Sc. and pulse c.mpression solut.ons for st.t.-of-t.e-art.yt.erbium laser t.c.3}ology.

关键词： pulse resonat.r mode

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3dc.P-Wh.at. An Open-Sourc. c.mpreh.nsive c.mput.t.onal Framework Prec.sely Quant.fies Wh.at.Foliar, Nonfoliar, and c.nopy Ph.t.synt.esis

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Plant.Ph.nomic. 2022年第1期4卷 156-174页

作者： tian-Gen c.1}ng Zai Sh. h.nglong Zh.o Qingfeng Song Zh.ngh. h. Jeroen Van Rie Bart.Den Boer Alex.nder Galle x.n-Guang zhu Nat.onal Key Laborat.ry for Plant.Molec.lar Genet.c. Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Insit.t. of c.op Sc.enc.s Chinese Academy of Agricultural SciencesBeijing 100081China Int.rnat.onal Maize and Wh.at.Improvement.c.nt.r(c.MMYt.c.2}na Offic. Chinese Academy of Agricultural SciencesBeijing 100081China BASF Belgium c.ordinat.on c.nt.r-Innovat.on c.nt.r Gent Technologiepark-Zwijnaarde 1019052 GentBelgium

c.nopy ph.t.synt.esis is t.e sum of ph.t.synt.esis of all above-ground ph.t.synt.et.c.*** roles of nonfoliar t.ssues in c.nopy ph.t.synt.esis remain elusive due t. met.odology ***,we develop t.e first.c.mplet. c.nopy ph.t.synt.esis model inc.rporat.ng all above-ground ph.t.synt.et.c.t.ssues and validat. t.is model on wh.at.wit. st.t.-of-t.e-art.gas ex.h.nge measurement.fac.lit.es.

关键词： Wh.at c.nopy ph.t.synt.esis

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Met.ormin improves boar sperm qualit. via 5′-AMPac.0}vat.d prot.in kinase-mediat.d energy met.bolism in vit.o

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Zoologic.l Researc. 2020年第5期41卷 527-538页

作者： Rong-Nan Li Zh.n-Dong zhu Yi Zh.ng Ying-h.a Lv x.u-E tian De Wu Yong-Jun Wang Wen-x.an Zeng Key Laborat.ny for Anima/Genet.c. Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYanglingShaanxi 712100China Key Laborat.ry for Animal Disease Resist.nc. Nut.it.on of t.e Minist.y of Educ.t.on of c.7}na Institute of Animal NutntionSichuan Agricultural UniversityChengduSichuan 611100China

Sperm are spec.alized c.lls t.at.require adenosine t.iph.sph.t.(At.) t. support.t.eir *** sperm energy h.meost.sis in vit.o is vit.lly import.nt.t. improve t.e effic.c. of boar sperm preservat.on. Met.ormin c.n ac.0}vat. 5′-AMPac.0}vat.d prot.in kinase(AMPK) t. improve met.bolic.flex.bilit. and maint.in energy h.meost.sis. t.us, t.e aim of t.e present.st.dy was t. invest.gat. wh.t.er met.ormin c.n improve boar sperm qualit. t.rough.AMPK mediat.on of energy met.bolism. Sperm mot.lit. paramet.rs, membrane int.grit.,ac.osomeint.grit.,mit.c.3}ndrial membrane pot.nt.al(ΔΨm), At. c.nt.nt. gluc.se upt.ke,and lac.0}t. efflux.were *** and ex.ression levels of AMPK and ph.sph.-t.r172-AMPK(p-AMPK) were also det.c.0}d by immunofluoresc.nc. and west.rn blot.ing. We found t.at.met.ormin t.eat.ent.signific.nt.y inc.eased sperm mot.lit. paramet.rs, ΔΨm, and At. c.nt.nt.during st.rage at.17 ℃. Moreover, result. sh.wed t.at.AMPK was loc.lized at.t.e ac.osomal region, c.nnec.0}ng piec., and midpiec. of sperm and p-AMPK was dist.ibut.d at.t.e post.ac.osomal region, c.nnec.0}ng piec., and midpiec.. Wh.n sperm were inc.bat.d wit. met.ormin for 4 h.at.37 ℃, sperm mot.lit. paramet.rs, ΔΨm, At. c.nt.nt.p-AMPK, gluc.se upt.ke, and lac.0}t. efflux.all signific.nt.y inc.eased, wh.reas t.e addit.on of c.mpound c.t.eat.ent. an inh.bit.r of AMPK,c.unt.rac.0}d t.ese posit.ve effec.0}. t.get.er, our result. suggest.t.at.met.ormin promot.s AMPK ac.0}vat.on, wh.c.3}c.nt.ibut.s t. t.e maint.nanc. of energy h.most.sis and mit.c.3}ndrial ac.0}vit.,t.ereby maint.ining boar sperm func.0}onalit. and improving t.e effic.c. of semen preservat.on.

关键词： Met.ormin AMPK Sperm Energy met.bolism Glyc.lysis

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