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Expression transformation of claudin-1 in the process of gastric adenocarcinoma invasion

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World Journal of Gastroenterology 2008年第31期14卷 4943-4948页

作者： Yong-Lian wu sheng Zhang Guo-Rong Wang Yu-peng Chen The Medical College of Putian University Putian 351100 Fujian Province China Department of Pathology the First Affiliated Hospital of Fujian Medical University Fuzhou 350005 Fujian Province China Hanjiang Hospital Putian 351100 Fujian Province China

AIM: To investigate the relation of expression transfor-mation of claudin-1 with invasiveness and metastasis of gastric carcinoma. METHODS: By using immunohistochemistry, expres-sion of claudin-1 in mucosa and invasive front of 136 gastric adenocarcinoma cases and proliferative index (Ki-67) were detected and analyzed. RESULTS: In mucosa, the claudin-1 over-expression rate of mucinous adenocarcinomas (including signet-ring cell carcinomas) was the highest. It was nega-tively related with the differentiation but positively related with the invasiveness and metastasis of gastric cancer. In invasive front, the claudin-1 over-expression rate was positively related with the differentiation, in-vasiveness and metastasis of gastric carcinoma. The expression transformation of claudin-1 was found in gastric carcinoma. The expression of claudin-1 in inva-sive front was transformed in 28/136 gastric carcinoma cases. The transformation rate in highly differentiated tubular adenocarcinomas was the highest (51.5%, 17/33). The deeper was the invasiveness, the higher was the transformation rate. The claudin-1 expression transformation rate in serosa and omenta was signifi -cantly higher (92.9%) than in tunica muscularis of in-vasive gastric cancer cases, as well as in patients withlymph node metastasis than in those without lymph node metastasis. CONCLUSION: Up-regulation of claudin-1 expres-sion and its transformation in invasive and metastatic gastric carcinoma suggest that claudin-1 participates in the transformation of biological behaviors in neo-plasms. Further study is needed to elucidate the pre-cise mechanism and the relation of claudin-1 expres-sion with the neoplasm progress.

关键词： Gastric carcinoma Claudin-1 Expression transformation Invasiveness Metastasis Immunohistochemistry

Molecular and biological characterization of melon-infecting squash leaf curl China virus in China

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Journal of Integrative Agriculture 2020年第2期19卷 570-577页

作者： wu Hui-jie LI Meng HONG Ni peng Bin GU Qin-sheng Zhengzhou Fruit Research Institute Chinese Academy of Agricultural SciencesZhengzhou 450009P.R.China Huazhong Agricultural University Wuhan 430070P.R.China School of Food and Bioengineering Zhengzhou University of Light IndustryZhengzhou 450000P.R.China

It has been reported that squash leaf curl China virus(SLCCNV)infects some Cucurbitaceae crops except for melon(Cucumis melo L.).A new disease of melon exhibiting severe leaf curl and dwarfing was observed in Hainan Province of *** this study,the pathogen was identified as SLCCNV through biological and molecular *** isolate(SLCCNV-HN)possess a bipartite genome,DNA-A(HM566112.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062251.1)pumpkin and SLCCNV-Hn61(AM260205.1)squash isolates from China,whereas DNA-B(HM566113.1)with the highest nucleotide identity(99%)to SLCCNV-Hn(MF062252.1).Phylogenetic analyses based on the full-length SLCCNV-HN DNA-A and-B sequences indicated that SLCCNV-HN melon isolate is clustered with SLCCNV-Hn pumpkin,SLCCNV-Hn61 and SLCCNV-SY squash isolates from southern China,forming an independent *** clone of SLCCNV-HN was constructed and the melon plants were inoculated and the infection rate is 100%,the systemic symptoms in melon showed identical to those of melon plants infected in ***,melon plants transmission of this virus by Bemisia tabaci with a transmission rate of 95%(19/20)showed leaf curl and dwarf symptoms 15 days post transmission,thereby fulfilling Koch’s *** of genomic organization and phylogenetic trees indicated that SLCCNV-HN melon isolate belongs to the Begomovirus *** the best of our knowledge,this is the first characterization of meloninfecting SLCCNV through its genome,infectious clone and transmission.

关键词： melon squash leaf curl China virus(SLCCNV) genome infectious clone

In situ synthesis of polycrystalline cubic boron nitride in cBN-Al-Ti system with different Gd_(2)O_(3) additions

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Rare Metals 2021年第5期40卷 1267-1274页

作者： sheng-Lin Zhong Zhi-Kai Li Chao Chen Chuan Chen peng Wang Yi wu College of Materials Science and Engineering Guilin University of TechnologyGuilin 541004China

Polycrystalline cubic boron nitride was sintered under high temperature(1400℃)and high pressure(5.5 GPa)using an in situ synthesis *** composition,microstructure,relative density and mechanical properties of the polycrystalline cubic boron nitride(PcBN)with different contents of Gd_(2)O_(3) were studied by means of X-ray diffraction(XRD),scanning electron microscopy(SEM)and energy-dispersive spectroscopy(EDS).The results show that the phases of TiB_(2),TiN,AIN,AlTi_(2)N and GdB_(6) are formed through in situ synthesis of cBN-Al-Ti-Gd_(2)O_(3) *** the Gd_(2)O_(3) content increasing,the relative density increases and mechanical properties of PcBN begin to increase and then *** amount of Gd_(2)O_(3) can advance the formation of TiB_(2) and improve the aspect ratio of TiB_(2).When Gd_(2)O_(3) content is 1.0 wt%,the PcBN exhibits optimal comprehensive mechanical properties,with a microhardness of 33.35 GPa and a flexural strength of 972.37 MPa.

关键词： Polycrystalline cubic boron nitride(PcBN) TiB_(2) Rod crystal Rare earth oxides Mechanical properties

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骨关节炎的氧化应激相关基因和免疫浸润分析

中国组织工程研究 2025年第2期29卷 302-311页

作者：吴傲于鹏滕加文孔鹏卞泗善山东中医药大学第一临床医学院山东省济南市250000 山东中医药大学附属医院山东省济南市250000

背景:目前对于骨关节炎的发病机制尚不清楚,缺乏有效控制疾病的手段,且研究多集中在免疫领域,对氧化应激领域研究较少。目的:探索氧化应激与免疫浸润在骨关节炎中的作用,并预测相关miRNA和治疗药物。方法:从GEO数据库中获取GSE55235数据... 详细信息

背景:目前对于骨关节炎的发病机制尚不清楚,缺乏有效控制疾病的手段,且研究多集中在免疫领域,对氧化应激领域研究较少。目的:探索氧化应激与免疫浸润在骨关节炎中的作用,并预测相关miRNA和治疗药物。方法:从GEO数据库中获取GSE55235数据集(10例骨关节炎样本和10例健康对照样本)和GSE55457数据集(10例骨关节炎样本和10例健康对照样本)进行合并,获取差异表达基因,并结合氧化应激基因得到差异表达氧化应激基因。对差异表达氧化应激基因进行KEGG、GO富集分析,并使用GSEA富集分析评价骨关节炎通路和生物过程。使用STRING在线平台和Cytoscape软件建立了蛋白质相互作用网络,运行Degree算法得到关键基因,从GEO数据库中获取GSE1919作为验证数据集,对关键基因进行差异分析及受试者工作特征曲线分析,得到核心基因。此外,用CIBERSORT对免疫浸润进行评估,并探索核心基因与免疫细胞的相关性,使用TargetScan对核心基因进行miRNA预测及使用DSigDB数据库对靶点药物进行预测。结果与结论:①确定了65个差异表达氧化应激基因和5个核心基因(IL1B、CXCL8、MYC、NFKBIA、JUN);②富集分析结果显示,差异表达氧化应激基因的主要聚焦点包括氧化应激、白细胞介素17、破骨细胞分化、流体剪切应力以及动脉粥样硬化等通路;③5个核心基因的受试者工作特征曲线下面积均>0.85,说明对诊断骨关节具有良好的特异性和敏感性,且与免疫细胞密切关联;④miRNA的预测结果是hsa-miR-3937,治疗小分子药物包括二甲双胍、离子霉素和塞来昔布等。结果表明:骨关节炎中存在氧化应激与免疫浸润,且免疫浸润参与激活氧化应激。核心基因及预测的miRNA可作为诊断骨关节炎的新型标志物,预测的小分子药物可治疗骨关节炎。

关键词：骨关节炎氧化应激免疫浸润差异基因生物标志物 miRNA 药物预测

Chinese public participation monitoring and warning system for geological hazards

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Journal of Mountain Science 2020年第7期17卷 1553-1564页

作者： wu sheng-nan LEI Yu CUI peng CHEN Rong YIN Pi-hua Key Laboratory of Land Surface Pattern and Simulation Institute of Geographical Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing10010China Key Laboratory of Mountain Hazards and Earth Surface Processes Institute of Mountain Hazards and EnvironmentChinese Academy of SciencesChengdu610041China University of Chinese Academy of Sciences Beijing100049China CAS Center for Excellence in Tibetan Plateau Earth Sciences Beijing100101China Liangshan Prefecture Geological Environmental Center Liangshan615000China Key Laboratory of Environmental Change and Natural Disaster Ministry of EducationBeijing Normal UniversityBeijing100875China Faculty of Geographical Science Academy of Disaster Reduction and Emergency ManagementBeijing Normal UniversityBeijing100875China

In China,many geological hazards occurred in remote mountainous regions,and it was time-consuming to disseminate disaster information for the responsible parties to make timely ***,only relying on professionals to monitor and manage disasters was demanding and *** Chinese government created a system to engage residents in the process of disaster risk management,namely’Public Participation Monitoring and Warning’(PPMW),to disseminate timely disaster information and bring down management *** objective of this system was to reduce casualties with minimum cost by organizing residents to evacuate from disasters in *** paper introduced the PPMW system,including its structure,operation mechanism by reviewing government documents and research articles,and its implementation by a case study of a landslide at Boli village(E 101°01’,N 27°29’),Yanyuan County,Sichuan Province,China on July 19th ***,this paper analyzed the strengths and limitations of the system and discussed its future *** had the potential to become an affordable disaster risk management tool for other countries facing similar situations to China.

关键词： Geological hazard Monitoring and early warning Disaster risk management Public participation Affordable solution

Fabrication of shish-kebab-structured carbon nanotube/poly(ε-caprolactone) composite nanofibers for potential tissue engineering applications

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Rare Metals 2019年第1期38卷 64-72页

作者： Tong wu Xin Chen Jin Sha Yi-Yan peng Yu-Lu Ma Lin-sheng Xie Lih-sheng Turng Engineering Center of Efficient Green Process Equipment and Energy Conservation Ministry of Education East China University of Science and Technology School of Mechanical and Power Engineering East China University of Science and Technology Wisconsin Institute for Discovery University of Wisconsin-Madison

The electrospinning process was applied to fabricate the nanofibers of biodegradable poly(ε-caprolactone)(PCL) in which different contents of multiwalled carbon nanotubes(MWCNTs) were embedded. Afterward,the electrospun nanofibers were successfully decorated with shish-kebab structure via a self-induced crystallization technique. The topographical features and the mechanical properties of the composite scaffolds were characterized,and the biocompatibility of the material was assessed by using human osteogenic sarcoma osteoblasts(MG-63 cells). The carbon nanotube(CNT) concentration is found to affect the fiber diameter and mechanical properties of electrospun nanofibers and the periodic distance of the shish-kebab architecture. Cellular attachment and proliferation assays reveal that 0.5 wt% CNT-embedded PCL scaffold shows enhanced biocompatibility with MG-63 cells than their counterparts made of neat PCL, and the collagen-like nanotopology provided by the shish-kebab structure further facilitates the cell adhesion and proliferation. The superior interactions between cells and scaffolds demonstrate that the shish-kebab-structured CNTs/PCL nanofibers may be promising candidate for tissue engineering scaffold application.

关键词： Carbon nanotubes Poly(ε-caprolactone) Electrospinning Shish-kebab structure Tissue engineering

Synthesis,Characterization of Polyethylene Ionomers and Their Antibacterial Properties

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Chinese Journal of Polymer Science 2024年第8期42卷 1077-1084页

作者： Jia-Jia wu Fei Wang peng-Qi Wan Li Pan Chun-sheng Xiao Zhe Ma Yue-sheng Li Tianjin Key Lab Composite&Functional Materials School of Materials Science and EngineeringTianjin UniversityTianjin 300350China Key Lab Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

Owing to its high production volume and wide range of application s,polyethylene has gained a great deal of attention,but its low surface energy and non-polar nature have limited its application in some important *** this study,ethylene/11-iodo-1-undecene copolymers were prepared and used as the intermediates to afford a series of imidazolium-based ionomers bearing methanesulfonate(CH_(3)SO_(3)^(-)),trifluoromethanesulfonate(CF_(3)SO_(3)^(-)),or bis(trifluoromethane)sulfonimide(Tf_(2)N^(-))*** tensile test results showed that the stress-at-break(7.8-25.6 MPa)and the elongation-at-break(445%-847%)of the ionomers could be adjusted by changing the counterion species and the ionic group *** importantly,the ionomers exhibited marvelous antibacterial activities against Staphylococcus aureus(***)and Escherichia coli(***).The ionomers bearing Tf_(2)N^(-)exhibited antibacterial activities>99%against both *** and *** when ionic content reached 9.1%.The imidazolium-based ionomers prepared in this work demonstrated excellent comprehensive properties,especially highefficient and broad-spectrum antibacterial ability,exhibiting the potential fo r the application as the antibacterial materials in packaging,medical,and other fields.

关键词： Polyethylene Ionomer Polymerization catalysis Imidazolium Antibacterial material

Novel high-performance element in the electromagnetic finite-element method——node-edge element

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Journal of Systems Engineering and Electronics 2008年第5期19卷 878-881页

作者： sheng Xinqing peng Zhen Center for Electromagnetic Simulation School of Information Science and Technology Beijing Inst. of Technology Beijing 100081 P. R. China

It is known in the computational electromagnetics （CEM） that the node element has a relative wellconditioned matrix, but suffers from the spurious solution problem; whereas the edge element has no spurious solutions, but usually produces an ill-conditioned matrix. Particularly, when the mesh is over dense, the iterative solution of the matrix equation from edge element converges very slowly. Based on the node element and edge element, a node-edge element is presented, which has no spurious solutions and better-conditioned matrix. Numerical experiments demonstrate that the proposed node-edge element is more efficient than now-widely used edge element.

关键词： node-edge element node element edge element matrix condition number.

A study of a supersonic capsule/rigid disk-gap-band parachute system using large-eddy simulation

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Applied Mathematics and Mechanics(English Edition) 2021年第4期42卷 485-500页

作者： sheng GONG Chuijie wu School of Aeronautics and Astronautics Dalian University of TechnologyDalian 116024Liaoning ProvinceChina

The aerodynamic performances and flow features of the capsule/rigid disk-gap-band(DGB)parachute system from the Mach number 1.8 to 2.2 are *** use the adaptive mesh refinement(AMR),the hybrid tuned center-difference and weighted essentially non-oscillatory(TCD-WENO)scheme,and the large-eddy simulation(LES)with the stretched-vortex subgrid *** simulations reproduce complex interaction of the flow structures,including turbulent wakes and bow shocks,as well as bow shocks and expansion *** results show that the calculated aerodynamic drag coefficient agrees well with the previou *** the aerodynamic drag coefficient and the aerodynamic drag oscillation of the parachute system decrease with the increase of the initial Mach number of the *** is found that the position and angle of the bow shock ahead of the canopy change as the Mach number increases,which makes the flow inside the canopy and the turbulent wake behind the canopy more complex and unstable.

关键词： compressible flow adaptive mesh refinement(AMR) large-eddy simulation(LES) supersonic flow rigid disk-gap-band(DGB)parachute