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DEVELOPMENTAL CAPACITIES OF THE GOLDFISH EGG, PROVIDED WITH ERYTHROCYTE NUCLEUS FROM ADULT CARP

Journal of Oceanology and Limnology 1982年第1期1卷 104-110页

作者： wu shangqin (S. C. wu), cai naner and xu quanhan (Institute of Oceanology, Academia Sinica) Institute of Oceanology Academia Sinica Qingdao China

1. Erythrocyte nucleus of adult carp was transplanted into enucleated egg of goldfish to test the totipotency of such differentiated cell.2. More than five thousand injections were made. Only 16% went on cleavage. They formed partial blastulae and blastulae. Only less than 1% went on gastrulation, but the completion of gastrulation was varied, from early epiboly to normal fish formation.3. 16 larvae with complete sets of organs were obtained, but gradually their development became abnormal, the body became distorted and edematous, and in 10—15 days they died without feeding ability.4. Three completely normal juvenile fishes have been obtained from such nucleo-cytoplasmic combination. Two of them died of fungus infestion at the age of 2 1/2 months. The remaining one is still alive now. The phenotypic characteristics of these fishes were of hybrid form. They were single tailed, with. elongated body and large scale as carp, and have the habit of jumping, but the form of the head and the absence of barbel were similar to goldfish. The characteristics of carp shown proves the presence of the function of the nucleus introduced.5. From the results presented above, we suggest that the nucleus of such differentiated cell like erythrocyte is still totipotent. The possible cause of such a low percentage of successful transplantation is also discussed.

关键词： phenotypic cytoplasmic varied juvenile larvae nucleus organs completion abnormal elongated

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NUCLEAR TRANSPLANTATION FOR SEVERAL GENERATIONS BETWEEN DIFFERENT VARIETIES OF GOLDFISH, CARASSIUS AURATUS

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Chinese Journal of Oceanology and Limnology 1983年第3期1卷 342-351页

作者： wu shangqin (S. C. wu), cai naner and xu quanhan (Institute of Oceanology, Academia Sinica, Qingdao) Institute of Oceanology Academia Sinica Qingdao

Three varieties of goldfish, the Red-dragon, the Red-carassius and the Eggfish, were used to demonstrate the influence of nucleus and cytoplasm on the phenotypic expression and their mutual interaction after nuclear transplantation. Fishes from these combinations could not only develop normally but also reach sexual *** characteristics of fishes after first transplantation （N1） were fundamentally of the nuclear donor variety e. g. the combination of nucleus of Red-dragon with cytoplasm of Redcarassius was very much similar to Red-dragon. The male fish of N1 mated with female Red-dragon gave offsprings with characters fundamentally like the Reddragon *** these blastulae were used as nuclear donor and transplanted their nuclei into Red-carassius cytoplasm again, we got the N2. There were more single tailed fishes in this category with the character of the Red-earassius. When the male fish of N2 mated with the Reddragon, more single tailed and atypical single tailed fishes appeared in their offsprings. This indicated that the changed characteristics in N2 was *** proceeded in this manner again and again, more and more single tailed and atypical single tailed fishes appeared in N3 and N4 and their offsprings when mated with Red-dragon. The effect of cytoplasm changed not only the phenotypic characters of the combination, but also the genetic information to next generation. Those influence was additive too.

关键词： CARASSIUS AURATUS NUCLEAR TRANSPLANTATION FOR SEVERAL GENERATIONS BETWEEN DIFFERENT VARIETIES OF GOLDFISH

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金鱼单尾鳍形成的研究——Ⅱ.胚胎发育过程中外源信息核糖核酸的作用

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海洋与湖沼 1989年第6期20卷 508-513页

作者：蔡难儿于富才吴贤汉徐权汉徐永立中国科学院海洋研究所

从鲤鱼卵巢中提取核酸(mRNA)注入不同发育阶段的金鱼胚胎中,观察mRNA对尾鳍发育的影响。观察结果表明:一次注射的或多次注射的,在胚胎早期均能使双尾鳍变成单尾鳍,但多次注射的,出现单尾鳍数要高;大剂量注射,甚至还可以使晚期胚胎(耳石... 详细信息

从鲤鱼卵巢中提取核酸(mRNA)注入不同发育阶段的金鱼胚胎中,观察mRNA对尾鳍发育的影响。观察结果表明:一次注射的或多次注射的,在胚胎早期均能使双尾鳍变成单尾鳍,但多次注射的,出现单尾鳍数要高;大剂量注射,甚至还可以使晚期胚胎(耳石期),也能形成单尾鳍;mRNA注入卵黄囊中所产生的单尾鳍鱼要比注入胚盘中为多;失活的mRNA不能诱导出单尾鳍的形成。从实验所得的资料表明:鲤鱼卵巢mRNA能够诱导双尾鳍金鱼转变成单尾鳍,但这种外源mRNA的作用性质,是由具有活性的mRNA翻译成蛋白质或者以它本身直接影响或干扰胚胎中某种“调节因子”,致使双尾鳍基因不能表达,从而导致单尾鳍的形成。

关键词：金鱼单尾鳍胚胎核糖核酸

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鲤鱼血红蛋白信息核糖核酸(HbmRNA)的分离

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海洋科学 1986年第6期 41-43页

作者：徐权汉徐永立蔡难儿中国科学院海洋研究所

信息核糖核酸(mRNA)是十分重要的生物活性物质。基因通过转录作用合成mRNA,再由mRNA翻译,编码特定的蛋白质顺序。因此,mRNA是基因和蛋白质之间的桥梁。不仅如此,某些病毒还可以用mRNA反转录编码DNA的程序。一些学者还发现某些高等动物

关键词： HbmRNA 血红蛋白缓冲液信息核糖核酸腹主动脉主动脉腹部网织红细胞上清液生物活性

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