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中国作物学会2013年学术年会

作者： Bantong Xue Jinlong Guo Youxiong Que Zhiwei Fu wu lu-guang Liping Xu Key Laboratory of Sugarcane Biology and Genetic Breeding Fujian Agriculture and Forestry UniversityMinistry of Agriculture School of Agriculture and Food Sciences University of Queensland

Transgene copy number has a great impact on the expression level and stability of exogenous gene in transgenic *** real-time PCR technique,which depends on the use of appropriate endogenous reference genes,has been widely accepted as an accurate and time-saved method in the copy number detection of transgenic plants and GM detection to meet the regulatory and legislative *** date,most transgene crops have been developed corresponding endogenous reference genes,some species have multiple applicable endogenous reference genes,but limited in ***,further development of exogenous reference genes for polyploid and aneuploid sugarcane is very *** this study,we evaluated a set of potential ’single copy’ genes including P4H,APRT,ENOL,CYC,TST and PRR,through qualitative PCR and absolute quantitative PCR. On the basis of copy number comparison in different sugarcane genotypes,including 5 Saccharum officinarum, one *** and two ***,the copy number of these endogenous genes fell into three groups:a high copy number group with only ENOL③,a medium copy number group containing TST①and PRR①,and a low copy number group with P4H③,APRT②and CYC②.Besides,the most stable reference genes were P4H, APRT and CYC,while the least stable genes were ENOL and TST in different sugarcane ***, primer pairs P4H③,APRT②and CYC②were selected as the suitable reference gene prime pairs in sugarcane. The test of multi-target reference genes plasmid on revealed that the APRT gene was a specific amplicon within the detection using the primers APRT②,and thus this gene was assumed to be the most suitable endogenous reference gene in *** results should be helpful for the analyses of the copy number of exogenous genes in transgenic sugarcane,and are also of vital significance for sugarcane transgenic breeding and its commercial application.

关键词： Sugarcane Endogenous reference genes qRT-PCR Copy number

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PREPARATION OF ULTRAFINE PARTICLE IRON-CARBONIDE CATALYST AND CHARACTERIZATION OF ITS CATALYTICAL BEHAVIOR

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Chinese Chemical Letters 1993年第3期4卷 275-278页

作者： Jing Chang ZHANG Ming Ming wu Jing Yun lu guang Sheng GUO Xiao Dong HE Fu Ming YANG Dept.of Appl.Chem.,Beijing Institute of Chemical Technology,Beijing 100029

Studies on ultrafine particle catalyst have attracted many researchers' attention by its large surface area,higher activity and *** on the mechanism of α-Fe and Fe_xC_y as the catalytical active species this paper reports for the first time the preparation method of Fe_3C ultrafine parti- cle catalyst,from highly dispersed amorphous Fe powder and free *** Fe powder and free car- bon,prepared by laser pyrolysis technique,was then treated by washing and heating at high tempera- ture protected with N_*** catalyst prepared under different experimental conditions was characterazed by means of XRD,electronic diffraction and *** shows that the crystlline grain size is in a range of 1-4nm and composed of Fe_3C and α-*** has been found that the ultrafine particle iron-carbonide catalyst exhibited much higher activity and selectivity to light *** the standard atmosphere and 380℃ reaction temperature,the conversion of CO reached a maximum of 80%.

关键词： PREPARATION OF ULTRAFINE PARTICLE IRON-CARBONIDE CATALYST AND CHARACTERIZATION OF ITS CATALYTICAL BEHAVIOR XRD Zhang ITS

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一种靶向S100A8的五聚体重组多肽疫苗的构建及其抗肿瘤活性研究

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药学学报 2016年第2期51卷 294-301页

作者：邱凌翀卢悟广李荣秀上海交通大学生命科学技术学院微生物代谢国家重点实验室上海200240

骨髓来源的抑制性细胞(myeloid derived suppressor cell,MDSC)在肿瘤免疫逃逸中扮演着重要的角色,普遍认为清除MDSC将有助于阻止肿瘤的生长。因此,本研究以霍乱毒素B亚基(CTB)五聚体为载体构建了一种靶向小鼠MDSC表面标识蛋白S100A8的... 详细信息

骨髓来源的抑制性细胞(myeloid derived suppressor cell,MDSC)在肿瘤免疫逃逸中扮演着重要的角色,普遍认为清除MDSC将有助于阻止肿瘤的生长。因此,本研究以霍乱毒素B亚基(CTB)五聚体为载体构建了一种靶向小鼠MDSC表面标识蛋白S100A8的重组多肽疫苗CTB-S100A8,并在大肠杆菌分泌表达系统中获得表达。经纯化后的重组CTB-S100A8五聚体蛋白辅以氢氧化铝佐剂免疫小鼠后能打破自身免疫耐受产生高滴度的S100A8抗体。在4T1小鼠乳腺癌肿瘤模型中,CTB-S100A8疫苗能够有效阻止肿瘤生长,并减少外周血中肿瘤诱导的单核细胞来源的MDSC。而正常的髓系来源MDSC、树突状细胞和巨噬细胞不受其影响。研究表明,CTB-S100A8是一种良好的靶向肿瘤诱导的MDSC的重组疫苗,具有较大的临床应用潜力。

关键词：免疫逃逸 S100A8 霍乱毒素B亚基蛋白疫苗骨髓来源的抑制性细胞

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Antinociceptive effects of analgesic-antitumor peptide（AGAP）,a neurotoxin from the scorpion Buthus martensii Karsch on formalin-induced inflammatory pain through a Mitogen-activated Protein Kinases-dependent Mechanism in mice

Antinociceptive effects of analgesic-antitumor peptide（AGAP...

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第十一届中国生物毒素研究及医药应用年会

作者： Qing-Hong Mao Jia-Ping Ruan wu-guang lu Xue-Ting Cai Juan Ye Jun-Li Cao Peng Cao Department of Anesthesiology Jiangsu Province Institute of Traditional Chinese Medicine Laboratory of Cellular and Molecular Biology Jiangsu Province Institute of Traditional Chinese Medicine Jiangsu Key Laboratory of Anesthesiology Department of AnesthesiologyAffiliated Hospital of Xuzhou Medical College

＜正＞In the present study,we investigated the anti-nociceptive effect and the underlying mechanism of the analgesic-antitumor peptide（AGAP）,a neurotoxin from the scorpion Buthus martensii *** in doses of 0.2,1 and 5 ug was intraplantar（***.） injected before formalin injection 10 min at the same *** suppression by intraplantar injection of AGAP on formalin-induced spontaneous nociceptive behaviors was *** results showed that AGAP could dose-dependent inhibit formalin-induced two-phase spontaneous flinching *** investigate the mechanism of action of treatment with AGAP in inflammatory pain,the expression of peripheral and spinal phosphorylated mitogen-activated protein kinases（phospho-MAPKs） including p-p38,p-ERK and p-JNK were *** found that formalin increased the expression of peripheral and spinal MAPKs,which were prevented by pre-intraplantar injection of AGAP in inflammation pain model in mice. Furthermore,AGAP could also decrease the expression of spinal Fos induced by formalin. The present results indicated that pre-intraplantar injection of AGAP prevented the inflammatory pain induced by formalin through a MAPKs-mediated mechanism in mice.

关键词： AGAP Formalin test Mitogen-activated protein kinases c-Fos Inflammatory pain

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A quantum-search-based multi-classifier for image recognition

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 21-31页

作者： lu Liu Xingyu wu lufan Zhang Chuan Wang School of Artificial Intelligence Beijing Normal University Applied Optics Beijing Area Major Laboratory Beijing Normal University

The multi-class classification of images is a pivotal challenge within the realm of image processing. As the volume of visual data continues to expand, there is a burgeoning interest in harnessing the unique capabilities of quantum computation to augment the efficiency of classification tasks. However, many existing methods for training quantum image multi-classifiers parallel classical machine learning techniques, where the requisite circuit measurements increase linearly with the volume of training data. This work introduces a novel approach for training a quantum image multi-classifier based on the quantum search algorithm. We have meticulously conducted rigorous experiments on a handwritten digit dataset, a classic benchmark in the field. The results have been meticulously compared with previous works, and the comparative analysis not only validates the efficiency of our proposed approach, requiring only O(N/b) measurements during training, but also highlights a significant quadratic speedup of the algorithm.

关键词： quantum computation image recognition quantum search algorithm

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白羊草主要生产性能构成因子分析

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中国草地学报 2013年第4期35卷 61-65页

作者：武路广霍梅俊董宽虎山西农业大学动物科技学院山西太谷030801

对采自山西省不同地区的10个白羊草居群的10个生产性能指标进行相关性分析和主成分分析,结果表明:10个指标中干草产量变异系数最大(29.90%),株丛密度最小(11.55%);白羊草不同居群间,除株丛密度外其他生产性能指标差异均达到极显著水平(P... 详细信息

对采自山西省不同地区的10个白羊草居群的10个生产性能指标进行相关性分析和主成分分析,结果表明:10个指标中干草产量变异系数最大(29.90%),株丛密度最小(11.55%);白羊草不同居群间,除株丛密度外其他生产性能指标差异均达到极显著水平(P<0.01);干草产量与株丛密度呈负相关,与花序重、小穗数、单株产量、单株茎重、单株叶重、结实率、茎叶比和千粒重均为正相关,单株产量与单株茎重、单株叶重呈极显著正相关(P<0.01)。主成分分析表明,前3个主成分因子中特征向量最大的分别为单株叶重、千粒重和小穗数,3个主成分因子累计贡献率达到78.609%。

关键词：白羊草生产性能相关分析主成分分析

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山西不同居群白羊草主要农艺性状评价

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草地学报 2013年第3期21卷 626-630页

作者：武路广霍梅俊董宽虎山西农业大学动物科技学院山西太谷030801

为筛选和合理利用白羊草(Bothriochloa ischaemum(L.)Keng)种质资源提供理论依据,利用主成分分析和聚类分析对采自山西省不同地区的10个白羊草居群的13个主要农艺性状进行分析和评价。结果表明:白羊草各居群间除小穗数外其他农艺性状差... 详细信息

为筛选和合理利用白羊草(Bothriochloa ischaemum(L.)Keng)种质资源提供理论依据,利用主成分分析和聚类分析对采自山西省不同地区的10个白羊草居群的13个主要农艺性状进行分析和评价。结果表明:白羊草各居群间除小穗数外其他农艺性状差异均达极显著水平(P<0.01)。主成分分析得到累计贡献率为86.927%的4个主成分,根据主成分分析结果对不同居群白羊草进行聚类分析,共得到4个类群,其中第1类群即临汾、芮城和太谷这3个白羊草居群综合表现最好。

关键词：白羊草农艺性状主成分分析聚类分析

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Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： Ming‑Yuan Yu Jing wu guang Yin Fan‑Zhen Jiao Zhong‑Zhen Yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

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Identifying the dynamic behaviors in complete reconstruction of Co-based complex precatalysts during electrocatalytic oxygen evolution

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Journal of Energy Chemistry 2025年第1期100卷 226-233页

作者： Jingfang Zhang Danyang wu Linke Cai Youluan lu Fanpeng Cheng Lijuan Shi Qun Yi Yao Liu Yi Huang Key Laboratory of Green Chemical Engineering Process of Ministry of Education Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education Hubei Key Laboratory of Novel Reactor and Green Chemical Technology School of Chemical Engineering & Pharmacy Wuhan Institute of Technology Key Laboratory of Pesticide & Chemical Biology of Ministry of Education Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction of Ministry of Education College of Chemistry Central China Normal University College of Materials Science and Engineering Fuzhou University

Transition metal-based nanomaterials have emerged as promising electrocatalysts for oxygen evolution reaction（OER）. Considerable research efforts have shown that self-reconstruction occurs on these nanomaterials under operating conditions of OER process. However, most of them undergo incomplete reconstruction with limited thickness of reconstruction layer, leading to low component utilization and arduous exploration of real catalytic mechanism. Herein, we identify the dynamic behaviors in complete reconstruction of Co-based complexes during OER. The hollow phytic acid（PA） cross-linked CoFe-based complex nanoboxes with porous nanowalls are designed because of their good electrolyte penetration and mass transport ability, in favor of the fast and complete reconstruction. A series of experiment characterizations demonstrate that the reconstruction process includes the fast substitution of PA by OH-to form Co（Fe）(OH)xand subsequent potential-driven oxidation to Co（Fe）OOH. The obtained CoFeOOH delivers a low overpotential of 290 mV at a current density of 10 mA cm-2and a long-term *** experiment results together with theory calculations reveal that the Fe incorporation can result in the electron rearrangement of reconstructed CoFeOOH and optimization of their electronic structure,accounting for the enhanced OER activity. The work provides new insights into complete reconstruction of metal-based complexes during OER and offers guidelines for rational design of high-performance electrocatalysts.

关键词： Complete reconstruction Co-based complex Precatalysts Oxygen evolution reaction

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二氢黄酮还原酶基因的克隆与转基因烟草的获得

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西北植物学报 2010年第1期30卷 1-7页

作者：王延秀张金文武禄光甘肃农业大学农学院兰州730070 澳大利亚昆士兰大学生命学院

以蒺藜苜蓿(Medicagotrunctulacv.5160)幼果总RNA为模板,采用RT-PCR技术克隆到二氢黄酮还原酶(DFR)基因的cDNA序列,所获得的cDNA序列全长1018 bp,具有完整的ORF,编码337个氨基酸。Blast分析表明,该片段与GenBank中注册的DFR基因同源性为... 详细信息

以蒺藜苜蓿(Medicagotrunctulacv.5160)幼果总RNA为模板,采用RT-PCR技术克隆到二氢黄酮还原酶(DFR)基因的cDNA序列,所获得的cDNA序列全长1018 bp,具有完整的ORF,编码337个氨基酸。Blast分析表明,该片段与GenBank中注册的DFR基因同源性为99.80%。以植物表达载体pBI121为基础,构建了Ca MV35S启动子驱动的DFR基因植物表达载体pBIDFR;采用直接转化法将pBIDFR导入根癌农杆菌EHA105,用该菌株对普通烟草进行遗传转化获得6株转基因植株。

关键词：二氢黄酮还原酶植物表达载体构建转化

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