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Effects of electroacupuncture at Taichong(LR 3) and Baihui(DU 20)on cardiac hypertrophy in rats with spontaneous hypertension

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Journal of Traditional Chinese Medicine 2019年第4期39卷 502-508页

作者： Ji Zhi Zhang Yue Liang Jingrong wu jiaojuan Jia Wenrui Wang Zhaoyang Liu Qingguo School of Acupuncture Moxibustion and TuinaBeijing University of Chinese MedicineBeijing 100029China Beijing University of Chinese Medicine Personnel DivisionBeijing 100029China School of Life Sciences Beijing University of Chinese MedicineBeijing 100029China

OBJECTIVE: To investigate the effects of electroacupuncture(EA) at Taichong(LR 3) and Baihui(DU 20)on myocardial hypertrophy in spontaneously hypertensive rats(SHRs).METHODS: Thirty-six SHRs were randomly assigned to model, EA, and Losartan groups, with twelve rats per group. Twelve Wistar Kyoto rats were selected as the normal control group. Systolic blood pressure(SBP) and cardiac function were measured in all *** levels of factors associated with the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PI3K/Akt/mTOR) pathway were evaluated by Western blotting and real-time *** changes of the heart tissue were observed by hematoxylin-eosin ***: After treatment, enhanced SBP was significantly decreased in the EA and Losartan groups compared with the model group(P < 0.01). Echocardiographic and morphological analyses revealed that enhanced end-diastolic interventricular septal thickness and left ventricular posterior wall thickness, as well as ratio of left ventricular weight to body weight were markedly diminished in the EA and Losartan groups(P < 0.01 or P < 0.05), while reduced left ventricular end-diastolic dimension and left ventricular ejection fraction were significantly ameliorated(P < 0.01). Real-time PCR and western blotting analyses showed that the expression levels of PI3K,Akt, and mT OR in SHRs were significantly up-regulated by EA and Losartan(P < 0.01), while the expression levels of PTEN and ANP were down-regulated(P < 0.01).CONCLUSION: EA at Taichong(LR 3) and Baihui(DU20) inhibited the development of cardiac hypertrophy and improved the cardiac function in SHRs, possibly through regulation of the PI3K/Akt/mTOR signalling pathway.

关键词： Electroacupuncture Rats,inbred SHR Hypertrophy Phosphatidylinositol 3-kinases Proto-oncogene proteins c-akt Mammalian target of rapamycin

基于压力感受器反射调控探讨针刺降压的作用机制

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上海中医药杂志 2024年第5期58卷 9-13页

作者：吴小丽吴娇娟刘宇张静张琳高辛瑜刘清国北京中医药大学北京100029 北京中医药大学东直门医院北京100029

当心输出量、外周阻力和血量等发生变化时,颈动脉窦和主动脉弓压力感受器反射是维持动脉血压稳定的重要神经反射机制,具有快速调节动脉血压的作用。研究表明针刺具有良好的降压作用,其降压机制可能与上调压力感受器的敏感性、促进神经... 详细信息

当心输出量、外周阻力和血量等发生变化时,颈动脉窦和主动脉弓压力感受器反射是维持动脉血压稳定的重要神经反射机制,具有快速调节动脉血压的作用。研究表明针刺具有良好的降压作用,其降压机制可能与上调压力感受器的敏感性、促进神经元传入信息增加、帮助中枢整合传入信号、兴奋迷走神经、抑制交感神经等有关。对针刺影响压力感受器反射的各环节功能而调节血压的作用机制进行阐述,以期为临床科研工作提供一定的参考。

关键词：高血压针刺压力感受器反射交感神经非药物疗法作用机制

Effects of different acupuncture manipulations on protein expression in the parietal cortex of spontaneously hypertensive rats

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Journal of Traditional Chinese Medical Sciences 2021年第3期8卷 257-264页

作者： jiaojuan wu Tianxiao Zeng Jingrong Liang Xudong Zhang Qi Xie Taotao Lv Pui Yee Wong Zhi Ji Qingguo Liu School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing102488China Department of Pain Management Huazhong University of Science and Technology Union Shenzhen HospitalShenzhen518052China Shu Guang TCM Healthcare Center KualaLumpur56000Malaysia Department of Acupuncture-Moxibustion China-Japan Friendship HospitalBeijing100029China

Objective: To analyze the effects of twirling reinforcing and reducing manipulations on protein expression in parietal cortex of spontaneously hypertensive rats(SHRs), and elucidate the main mechanisms and differences between two manipulations in hypertension ***: Rats were randomly divided into the control, model, twirling reinforcing manipulation(TRFM),and twirling reducing manipulation(TRDM) groups. The control and model groups received catch and fixation stimulations once a day for 14 days. The TRFM and TRDM groups were intervened once a day for 20 min for 14 days. On days 0, 2, 6, 10, and 14 after acupuncture, rat systolic blood pressures(SBPs) were measured. Differential protein(DP) expression in the rat parietal cortices was detected. Thereafter, GO functional significance and KEGG pathway enrichment analyses were ***: Compared with the model group, SBP of rats in the TRDM and TRFM groups decreased on days 6 and 10 of acupuncture, respectively(P=.009;P <.001). Moreover, SBP of the TRDM group was significantly lower than that of the TRFM group on days 10 and 14 of acupuncture(P=.015;P=.013).Compared with control group, 601 and 1040 DPs were up-and downregulated, respectively, in the model group. Compared with model group, 44 and 28 up-and downregulated DPs were expressed, respectively,in the TRFM group. Compared with model group, expression of 616 and 427 up-and downregulated DPs,respectively, was found in the TRDM group. After combining the results of GO and KEGG enrichment analyses, five and one pathways were found to be related to the central antihypertensive mechanism of the parietal cortex during twirling reducing and reinforcing manipulations, ***: TRDM showed a more effective antihypertensive effect on SHRs than TRFM;this antihypertensive effect was related to the regulation of different proteins and their biological functions.

关键词： Hypertension Proteomics Parietal cortex Acupuncture Manipulation

Effect of electroacpuncture of Neiguan(PC 6)and Tianquan(PC 2)on skin temperature,blood perfusion,and adrenoceptor response in rats with acute myocardial ischemia

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Journal of Traditional Chinese Medicine 2017年第6期37卷 794-803页

作者： Wang Dan Ji Bo Zhao Guozhen Yan Mingna Bai Hongxin Sun Xiaomin Dai Jian Lu Yawen Liu Yitian You Min wu jiaojuan Ge Yunpeng Su Hang Cheng Kai College of Acupunc-ture and Massage Beijing University of Traditional Chinese MedicineBeijing 100029China Clinical medical college Beijing University of Traditional Chinese MedicineBeijing 100029China

OBJECTIVE: To investigate the effect of electroacupuncture(EA) of acupoints Neiguan(PC 6) and Tianquan(PC 2) on the skin temperature, blood perfusion, and alpha1-and beta2-adrenoreceptor(α1-AR and β2-AR) protein and m RNA level in rats with acute myocardial ***: Thirty male adult Wistar rats [(230 ±20) g] were randomly assigned into five groups(n = 6 each): a control group, sham operation group, model group, model group treated with EA at low frequency(L-EA, 2 Hz, 1 m A) and model group treated with EA at high frequency(H-EA, 100 Hz, 1 m A). The rat model was prepared by ligating the left anterior descending coronary artery. Electroacupuncture was performed at the left Neiguan(PC 6) for 20 min daily for 3 d. After the 3 rd time of the treatment, measurements of skin blood perfusion and temperature in Neiguan(PC 6) and Tianquan(PC 2) in all groups were made by laser speckle contrast imaging and infrared thermal image instrument. Real-time PCR and ELISA were used to measure m RNA level and protein level of α1-AR andβ2-AR in the skin tissues of Neiguan(PC 6) and Tianquan(PC 2), respectively. Serum levels of c Tn T and electrocardiogram were used to identify the state of ***: In the group receiving electroacupunture at Neiguan(PC 6), compared with control group,the skin temperature, blood perfusion, and β2-AR m RNA and protein level of model group significantly decreased(P < 0.05), α1-AR m RNA and protein level of model group significantly increased(P < 0.05);compared with model group, the skin temperature,blood perfusion, and β2-AR m RNA and protein level of L-EA significantly increased(P < 0.05), α1-AR m RNA and protein level of L-EA and H-EA significantly decreased(P < 0.05). The same trend has taken place in the former four groups of the Tianquan(PC 2).CONCLUSION: Low-or high-frequency electroacupuncture can improve the skin temperature and blood perfusion which may be induced by decreasingthelevelofα1-ARandincreasingthelevelofβ2-AR of th

关键词： Electroacupuncture Myocardial isch-emia Point PC 6 (Neiguan), Point PC 2 (Tian- quan), Skin temperature Skin blood perfusion,Adrenoceptor

电针“太冲”对自发性高血压大鼠海马MDA、SOD、GSH-Px含量的影响

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中国中医基础医学杂志 2022年第9期28卷 1439-1442页

作者：薛艳君吴娇娟张旭东蔚鹏程吴小丽刘清国北京中医药大学针灸推拿学院北京100029

目的:通过观察电针“太冲”对自发性高血压大鼠(spontaneously hypertensive rats,SHR)海马损伤的改善情况,探讨电针治疗高血压的中枢机制。方法:按照随机数字表法将12只雄性SHR大鼠分为电针组与模型组2组各6只,6只雄性WKY大鼠为空白组... 详细信息

目的:通过观察电针“太冲”对自发性高血压大鼠(spontaneously hypertensive rats,SHR)海马损伤的改善情况,探讨电针治疗高血压的中枢机制。方法:按照随机数字表法将12只雄性SHR大鼠分为电针组与模型组2组各6只,6只雄性WKY大鼠为空白组,适应性喂养1周后电针组用电针仪分别连接双侧“太冲”穴,电流强度1 mA,频率2 Hz,留针20 min连续针刺14 d,每日1次。实验第15天,HE染色法观察海马组织的形态结构,并测定海马组织中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量。结果:与空白组比较,模型组HE染色明显变浅,出现细胞溶解碎裂、核固缩、空泡样变性等;与模型组比较,电针组细胞染色加深,核仁较清晰,细胞结构基本正常;与空白组比较,模型组的MDA含量显著提升(P<0.01),SOD、GSH-Px含量显著降低(P<0.01);与模型组比较,电针组MDA含量明显下降(P<0.05),SOD、GSH-Px含量明显上升(P<0.05)。结论:电针“太冲”穴能通过调节SHR大鼠海马中MDA、SOD和GSH-Px的表达水平,抑制氧化反应,减轻氧自由基对SHR大鼠海马组织的损伤,这可能是电针“太冲”穴对高血压的中枢保护机制之一。

关键词：电针高血压海马 MDA SOD GSH-Px

针刺捻转补泻法对自发性高血压大鼠海马丙二醛、谷胱甘肽过氧化物酶含量的影响

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中国医药导报 2021年第17期18卷 4-7,17页

作者：薛艳君吴娇娟张旭东蔚鹏程吴小丽刘清国北京中医药大学针灸推拿学院北京100029

目的探讨针刺捻转补泻法对自发性高血压大鼠海马组织形态及海马中丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)含量的影响。方法18只自发性高血压大鼠按照随机数字表法分为三组:泻法组、补法组与模型组,每组6只。6只WKY大鼠为空白组。所有... 详细信息

目的探讨针刺捻转补泻法对自发性高血压大鼠海马组织形态及海马中丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)含量的影响。方法18只自发性高血压大鼠按照随机数字表法分为三组:泻法组、补法组与模型组,每组6只。6只WKY大鼠为空白组。所有大鼠均为SPF级9周龄雄性大鼠。适应性喂养1周后,泻法组和补法组采用相应手法针刺双侧太冲穴14 d,1次/d。模型组与空白组进行与泻、补法组同等位置的抓取固定刺激。第15天,采用HE染色法观察海马组织,硫代巴比妥酸法检测MDA含量,比色法检测GSH-Px含量。结果HE染色结果显示,空白组细胞结构完整,形态规则,细胞间隙正常,多数细胞核深染,核仁清晰,未见变性坏死。与空白组比较,模型组染色明显变浅,出现核固缩、细胞溶解碎裂、空泡样变性和凋亡细胞。与模型组比较,泻法组和补法组均可见细胞轮廓较清晰,细胞染色加深,核仁清晰,细胞结构基本正常。模型组的MDA含量高于空白组,GSH-Px含量低于空白组,差异有高度统计学意义(P<0.01)。泻法组、补法组MDA含量均低于模型组,GSH-Px含量均高于模型组,差异有统计学意义(P<0.05或P<0.01)。结论针刺捻转补泻法能通过调节自发性高血压大鼠海马中MDA和GSH-Px的表达水平,抑制氧化反应,减轻氧自由基对其组织损伤,这可能是针刺捻转补泻法对高血压海马损伤的保护机制之一。

关键词：针刺捻转补泻法高血压海马丙二醛谷胱甘肽过氧化物酶

电针三阴交对AD小鼠学习记忆能力及海马区Ach、AchE、ChAT的影响

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长春中医药大学学报 2017年第2期33卷 181-184页

作者：翟丽静王婧秦卓游敏吴娇娟李昱颉程凯北京中医药大学针灸推拿学院北京100029

目的观察电针"三阴交穴"对SAMP8快速衰老模型小鼠学习记忆能力和脑组织海马区内乙酰胆碱(Ach)、乙酰胆碱酯酶(Ach E)、乙酰胆碱转移酶(Ch AT)的影响。方法将快速老化SAMP8小鼠随机分为模型组和电针组,并设同源SAMR1小鼠作为对照组,每组... 详细信息

目的观察电针"三阴交穴"对SAMP8快速衰老模型小鼠学习记忆能力和脑组织海马区内乙酰胆碱(Ach)、乙酰胆碱酯酶(Ach E)、乙酰胆碱转移酶(Ch AT)的影响。方法将快速老化SAMP8小鼠随机分为模型组和电针组,并设同源SAMR1小鼠作为对照组,每组均为6只。电针组给予三阴交电针干预;对照组,模型组常规抓取,不进行针刺。采用Morris水迷宫法检测小鼠的学习记忆能力;酶联免疫法(ELISA)检测小鼠海马部位的乙酰胆碱含量、乙酰胆碱酯酶和乙酰胆碱转移酶活性。结果 Morris水迷宫定位航行实验和空间探索实验显示:较SAMR1小鼠相比SAMP8小鼠学习记忆能力显著下降(P<0.01),电针治疗后明显改善(P<0.05),差异有统计学意义。指标显示:模型组较对照组海马内AchE、ChAT活性明显降低,Ach含量显著减少(P<0.01),电针治疗后AchE、ChAT活性明显升高,且Ach含量明显增多(P<0.05),差异有统计学意义。结论电针三阴交穴可以明显改善老年痴呆模型小鼠学习记忆能力并提高海马区乙酰胆碱含量,其作用可能是通过改善脑组织海马区乙酰胆碱酯酶和乙酰胆碱转移酶活性来实现的。

关键词：老年痴呆穴,三阴交学习记忆能力乙酰胆碱乙酰胆碱酯酶乙酰胆碱转移酶

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VR技术应用于经络腧穴学教学的探索研究

中国中医药现代远程教育 2020年第5期18卷 1-3页

作者：林怡程凯彭超游敏吴娇娟杨守亲古春凌北京中医药大学针灸推拿学院北京100029

目的初探经络腧穴学VR教学对不同年级学生的教学效果。方法根据前期问卷调查结果选定教学试验的内容,面向北京中医药大学针灸推拿学院针灸推拿专业征集48名本科生和研究生(大一年级的本科生18人,大三年级的本科生20人,研究生10人),用分... 详细信息

目的初探经络腧穴学VR教学对不同年级学生的教学效果。方法根据前期问卷调查结果选定教学试验的内容,面向北京中医药大学针灸推拿学院针灸推拿专业征集48名本科生和研究生(大一年级的本科生18人,大三年级的本科生20人,研究生10人),用分层分组的方式将其分为传统教学组和VR教学组,每组各24人。2组运用不同的教学方式进行相同内容的教学,并在教学结束后进行试卷考核。结果VR组研究生试卷得分均值大于传统教学组,大一年级和大三年级学生试卷得分均值小于传统教学组,但以上差异均无统计学意义(P>0.05)。传统教学组内不同年级学生试卷得分差异无统计学意义(P>0.05);VR教学组内不同年级学生试卷得分差异有统计学意义(P0.05)。结论从学生的试卷成绩分析上来看,尚不能认为VR教学与传统教学的教学效果不同;VR教学不适合直接应用于大一年级尚未构建经络、解剖知识框架的学生,或更适合学生在具备基本的经络腧穴学知识与系统解剖学知识后进行。

关键词：经络腧穴学虚拟现实技术教学改革

针刺对自发性高血压大鼠心肌ANP、ACE2表达和Ang(1-7)/AngⅡ比值的影响

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中国医药导报 2019年第17期16卷 4-8页

作者：纪智袁静云王紫娟张跃梁靖蓉吴娇娟刘清国北京中医药大学针灸推拿学院

目的通过观察自发性高血压大鼠(SHR)心肌组织结构和肾素-血管紧张素-醛固酮系统(RAS)作用因子表达的变化,探究针刺对高血压心肌肥厚的防护机制。方法将10只WKY大鼠作为空白组,将20只SHR按照随机数字表法分为模型组和针刺组,每组各10只... 详细信息

目的通过观察自发性高血压大鼠(SHR)心肌组织结构和肾素-血管紧张素-醛固酮系统(RAS)作用因子表达的变化,探究针刺对高血压心肌肥厚的防护机制。方法将10只WKY大鼠作为空白组,将20只SHR按照随机数字表法分为模型组和针刺组,每组各10只。所有大鼠均为10周龄SPF级雄性大鼠。适应性饲养1周后,针刺组大鼠用毫针于“百会”和双“太冲”直刺,每天1次,连续干预28 d。模型组和空白组不做针刺干预。于实验第1、7、14、21、28天针刺前测量血压值。实验结束后称取大鼠全心及左心室重量,并计算心脏重量指数(HWI)和左心室重量指数(LVWI)。应用HE和Masson染色法观察心肌组织形态,并通过Real-tiem PCR、Western blot、酶联免疫吸附测定(ELISA)等技术检测各组大鼠心肌组织中心房钠尿肽(ANP)、血管紧张素转换酶2(ACE2)、血管紧张素1-7[Ang(1-7)]和AngⅡ的表达,并计算Ang(1-7)/AngⅡ。结果与空白组比较,模型组大鼠收缩压(SBP)、HWI、LVWI显著升高(P < 0.01)。与模型组比较,针刺组大鼠SBP、HWI、LVWI值明显降低(P < 0.05或P < 0.01)。病理切片结果显示,与空白组比较,模型组大鼠心肌细胞的细胞体积明显变大,且排列相对紊乱,同时细胞间距变宽,充斥着大量被蓝染的纤维。与模型组比较,针刺组大鼠心肌细胞体积更小,细胞间距更窄,细胞间隙蓝染的纤维变少。与空白组比较,模型组心肌组织的ACE2的表达水平和Ang(1-7)/AngⅡ显著降低(P < 0.01),ANP的表达水平升高(P < 0.01)。与模型组比较,针刺组ACE2的表达水平和Ang(1-7)/AngⅡ的比值显著升高(P < 0.01),ANP的表达水平降低(P < 0.01)。结论针刺“百会”“太冲”可通过调节ACE2/Ang(1-7)的表达,有效降低SHR血压,并减轻其心肌肥厚的程度。

关键词：高血压针刺心肌肥厚血管紧张素转换酶2 血管紧张素1-7