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嵌入学习效应的多目标应急伤员手术调度

智能计算机与应用 2024年第2期14卷 76-82页

作者：吴丹妮叶春明上海理工大学管理学院上海200093

为了合理高效地解决突发事件后医院手术室资源调度问题,本文在经典车间调度模型的基础上,提出了应急手术调度模型。以手术室救治总成本最低以及伤员的手术完成时间最短为目标,重点考虑了学习效应对手术完成时间的影响,并对比分析了不同... 详细信息

为了合理高效地解决突发事件后医院手术室资源调度问题,本文在经典车间调度模型的基础上,提出了应急手术调度模型。以手术室救治总成本最低以及伤员的手术完成时间最短为目标,重点考虑了学习效应对手术完成时间的影响,并对比分析了不同学习因子下的调度结果。利用带有精英策略的非支配排序遗传算法求解多目标手术调度问题,并通过仿真对比实验证明了模型和算法的有效性。

关键词：手术调度学习效应多目标手术调度非支配排序遗传算法

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畜禽粪便源有机肥中四环素类抗生素及转化产物的分布特征

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环境化学 2024年第5期43卷 1445-1454页

作者：吴丹妮冯皓迪代世婷马春萌杨敏张昱郑州大学河南先进技术研究院郑州450003 中国科学院生态环境研究中心环境水质学国家重点实验室北京100085 北京交通大学市政与环境工程系北京100044 中国科学院大学北京100049

畜禽粪便源有机肥是农田土壤中抗生素母体及转化产物污染的重要来源之一.为研究畜禽粪便源有机肥中四环素类抗生素及转化产物的残留情况,在全国8个省(市)采集55个有机肥样品,采用超高效液相色谱-串联质谱法(UPLC-MS/MS),分析畜禽粪便源... 详细信息

畜禽粪便源有机肥是农田土壤中抗生素母体及转化产物污染的重要来源之一.为研究畜禽粪便源有机肥中四环素类抗生素及转化产物的残留情况,在全国8个省(市)采集55个有机肥样品,采用超高效液相色谱-串联质谱法(UPLC-MS/MS),分析畜禽粪便源有机肥中四环素、强力霉素、金霉素和土霉素4种四环素类抗生素及10种转化产物的分布特征.结果表明,四环素、强力霉素、金霉素和土霉素在55个有机肥样品中均有检出,浓度分别为1.63-4348μg·kg^(-1)、ND-11451μg·kg^(-1)、ND-30300μg·kg^(-1)、7.11-55008μg·kg^(-1).10种转化产物中,差向异构体是主要的转化产物.不同畜禽粪便来源的有机肥中四环素类抗生素及转化产物的浓度存在显著差异,猪粪肥样品中四环素类抗生素及转化产物的浓度高于鸡粪肥、牛粪肥和羊粪肥.畜禽粪便资源化利用模式可能也会影响有机肥中四环素类抗生素的浓度.种养结合模式生产的有机肥中四环素类抗生素及转化产物的浓度显著高于集中处理模式生产的有机肥.进一步研究了典型地区畜禽粪便源有机肥和施肥土壤中的四环素类抗生素及转化产物残留的污染特征,发现四环素类抗生素及转化产物通过施用有机肥进入土壤环境中,差向异构体在施肥土壤中仍是主要的转化产物.畜禽粪便源有机肥和施肥土壤中四环素类抗生素及转化产物的残留不容忽视.

关键词：畜禽粪便源有机肥施肥土壤四环素类抗生素转化产物

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Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase

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National Science Review 2023年第8期10卷 78-87页

作者： Huawei Tong Nana Liu Yinghui Wei Yingsi Zhou Yun Li danni wu Ming Jin Shuna Cui Hengbin Li Guoling Li Jingxing Zhou Yuan Yuan Hainan Zhang Linyu Shi Xuan Yao Hui Yang HuidaGene Therapeutics Co. Ltd. Department of Neurology and Institute of Neurology of First Affiliated Hospital Institute of Neuroscience and Fujian Key Laboratory of Molecular Neurology Fujian Medical University Institute of Neuroscience Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences Shanghai Center for Brain Science and Brain-Inspired Intelligence

Current DNA base editors contain nuclease and DNA deaminase that enables deamination of cytosine(C)or adenine(A), but no method for guanine(G) or thymine(T) editing is available at present. Here we developed a deaminase-free glycosylase-based guanine base editor(gGBE) with G editing ability, by fusing Cas9 nickase with engineered N-methylpurine DNA glycosylase protein(MPG). By several rounds of MPG mutagenesis via unbiased and rational screening using an intron-split EGFP reporter, we demonstrated that gGBE with engineered MPG could increase G editing efficiency by more than 1500 ***, this gGBE exhibited high base editing efficiency(up to 81.2%) and high G-to-T or G-to-C(i.e. G-to-Y) conversion ratio(up to 0.95) in both cultured human cells and mouse embryos. Thus, we have provided a proof-of-concept of a new base editing approach by endowing the engineered DNA glycosylase the capability to selectively excise a new type of substrate.

关键词： glycosylase base excision repair base editor depurination deaminase-free transversion

Determination of Salt Impurities in MDEA Solution Used in Desulfurization of Highly Sulphurous Natural Gas

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China Petroleum Processing & Petrochemical Technology 2015年第3期17卷 25-31页

作者： Liu Yucheng Zhang Bo Chen Mingyan wu danni Zhou Zheng School of Chemistry and Chemical Engineering Southwest Petroleum University Puguang Branch of Zhongyuan Oilfield Company

The foaming phenomenon of N-methyldiethanolamine(MDEA) solution used in desulfurization process occurs frequently in the natural-gas purification plant. The foaming phenomenon has a strong impact on operation of the process unit. The salt impurities are the main reason for causing the foaming of MDEA solution, so the full analysis of salt impurities is necessary. A method for comprehensive analysis of salt impurities in MDEA solution used in desulfurization process was established. Anions and non-metallic cations of MDEA solution were determined by different conditions of ion chromatograph, respectively. Metallic cations of the solution were detected by atomic absorption spectrophotometer with the N2O-C2H2 flame absorption. The analytical results of salt impurities in the desulfurization solution can provide a theoretical basis for an accurate analysis of the factors affecting the foaming of MDEA to unveil further control measures.

关键词： natural-gas purification plant MDEA solution salt impurities full analysis ion chromatograph

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Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma

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Engineered Regeneration 2024年第2期5卷 199-209页

作者： Feng Liang Xueying An Ruoxi Wang Wenshu wu Lin Yang Yixin Zheng Qing Jiang Xingquan Xu danni Zhong Min Zhou Eye Center The Second Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China Department of Orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008PR China Zhejiang University-University of Edinburgh Institute(ZJU-UoE Institute) Zhejiang University School of MedicineZhejiang UniversityHaining314400PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalClinical College of Nanjing Medical UniversityNanjing210008PR China Institute of Translational Medicine Zhejiang UniversityHangzhou310029PR China Zhejiang University-Ordos City Etuoke Banner Joint Research Center Zhejiang UniversityHaining314400PR China

Osteosarcoma(OS)is one of the most common malignant tumors in children and young *** chemotherapy and other therapies are limited by low therapeutic efficiency,severe side effects and single therapeutic function,it is of high value to develop innovative therapies for precise and efficient treatment of ***,natural photo-synthetic microalgae(***,CV)were utilized as carriers for the chemotherapeutic agent doxorubicin(DOX)to create a multifunctional therapeutic platform(CV@DOX)for the photo-modulation of the tumor microenvi-ronment(TME)and synergistic chemo-photodynamic therapy of ***@DOX exhibited rapid drug release behavior in the acidic TME,improving the efficiency of chemotherapy against tumors and reducing side effects on other normal *** 650 nm laser irradiation,CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species(ROS),thereby enhancing photodynamic therapy(PDT).CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis,inhibiting tumor proliferation and ***,chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after ***,CV@DOX did not cause significant hematological and tissue toxicity,and prevented DOX-induced cardiotoxicity,showing good in vivo ***,this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosar-coma treatment.

关键词： Osteosarcoma Tumor hypoxia Photodynamic Chemotherapy Synergistic therapy

Naked-eye visualization of lymph nodes using fluorescence nanoprobes in non-human primate-animal models

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Nano Research 2024年第8期17卷 7404-7414页

作者： Xiaoyuan Ji Binbin Chu Xiaofeng wu Zhiming Xia Airui Jiang Chenyu Wang Zhiming Chen danni Zhong Qiaolin Wei Bin Song Wanlin Li Yiling Zhong Houyu Wang Fenglin Dong Min Zhou Yao He Suzhou Key Laboratory of Nanotechnology and Biomedicine Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesInstitute of Functional Nano and Soft Materials(FUNSOM)Soochow UniversitySuzhou 215123China School of Public Health Shanghai Jiao Tong University School of MedicineShanghai 200025China Zhejiang University-University of Edinburgh Institute(ZJU-UoE Institute) Zhejiang UniversityHaining 314400China Department of Ultrasound the First Affiliated Hospital of Soochow UniversitySuzhou 215006China State Key Laboratory(SKL)of Biobased Transportation Fuel Technology Zhejiang UniversityHangzhou 310027China School of Pharmacy Hangzhou Normal UniversityHangzhou 311121China Macao Translational Medicine Center Macao University of Science and TechnologyTaipa 999078MacaoChina Macao Institute of Materials Science and Engineering Macao University of Science and TechnologyTaipa 999078MacaoChina

Despite sufficient studies performed in non-primate animal models,there exists scanty information obtained from pilot trials in non-human primate animal models,severely hindering nanomaterials moving from basic research into clinical *** herein present a pioneering demonstration of nanomaterials based optical imaging-guided surgical operation by using macaques as a typical kind of non-human primate-animal ***,taking advantages of strong and stable fluorescence of the small-sized(diameter:~5 nm)silicon-based nanoparticles(SiNPs),lymphatic drainage patterns can be vividly visualized in a real-time manner,and lymph nodes(LN)are able to be sensitively detected and precisely excised from small animal models(e.g.,rats and rabbits)to non-human primate animal models(e.g.,cynomolgus macaque(Macaca fascicularis)and rhesus macaque(Macaca mulatta)).Compared to clinically used invisible near-infrared(NIR)lymphatic tracers(i.e.,indocyanine green(ICG);etc.),we fully indicate that the SiNPs feature unique advantages for naked-eye visible fluorescence-guided surgical operation in long-term *** toxicological analysis in macaque models further provides confirming evidence of favorable biocompatibility of the SiNPs *** expect that our findings would facilitate the translation of nanomaterials from the laboratory to the clinic,especially in the field of cancer treatment.

关键词： fluorescent silicon nanoparticles lymph node real-time naked-eye visualization macaque models

Effects of Sodium Cyclohexyl Sulfamate on Growth and Development of Drosophila melanogaster

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Agricultural Biotechnology 2024年第4期13卷 65-69页

作者： Lei WEI danni MA Guobao JIANG Jiayu wu Na FENG College of Life Science and Food Engineering Shaanxi Xueqian Normal UniversityXi'an 710100China

[Objectives]This study was conducted to investigate the effects of different concentrations of sodium cyclohexyl sulfamate on the growth and development of Drosophila melanogaster.[Methods]Different concentrations of sodium cyclohexyl sulfamate were added to the culture medium,and the effects of different concentrations of sodium cyclohexyl sulfamate on the development time and weight of *** in various life stages were statistically analyzed.[Results]High concentration of sodium cyclohexyl sulfamate delayed the time of pupation and eclosion of ***,which made *** lose *** number of male *** in the first generation was much larger than that of female individuals,which indicated that the effect of sodium cyclohexyl sulfamate on male *** was greater than that of female *** a word,high concentration of sodium cyclohexyl sulfamate significantly inhibited the growth and development of ***.[Conclusions]This study provides some reference data for the research perspective of food additives and the safe use of sodium cyclohexyl sulfamate.

关键词： Drosophila melanogaster Sodium cyclohexyl sulfamate Pupation Eclosion

后现代图画书中的母女关系与多维度阐释——以约翰·伯宁罕的“莎莉系列”为例

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昆明学院学报 2022年第1期44卷 18-23页

作者：吴丹妮华中科技大学外国语学院湖北武汉430074

在《莎莉,离水远一点》《莎莉,洗好澡了没?》中,莎莉作为一个女孩,现实中必须服从于成人的权威,习得社会所期待的女性行为规范。但在她的幻想世界中,莎莉机智、勇敢、充满冒险精神。莎莉的母亲是压迫者,致力于培养女儿符合社会所期待的... 详细信息

在《莎莉,离水远一点》《莎莉,洗好澡了没?》中,莎莉作为一个女孩,现实中必须服从于成人的权威,习得社会所期待的女性行为规范。但在她的幻想世界中,莎莉机智、勇敢、充满冒险精神。莎莉的母亲是压迫者,致力于培养女儿符合社会所期待的女性角色;同时她也是被压迫者,挣扎于满足母亲的角色却又力不从心。约翰·伯宁罕的"莎莉系列"图画书以开放式的文字与图像叙事将人物关系复杂化,虚幻与现实相互交融,含混的图文关系给读者提供了无限丰富的阅读体验。

关键词：儿童文学后现代图画书约翰·伯宁罕女性主义图文关系

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郭熙山水画在当代山水画中的应用

陶瓷 2023年第8期 85-87,114页

作者：吴丹妮景德镇陶瓷大学江西景德镇333403

笔者通过了解郭熙的生平背景,并对他的绘画风格和山水画绘画技法进行研究,包括郭熙用笔、用墨技法特点以及“三远法”的构图形式。阐述了郭熙《林泉高致》中提到的部分绘画理论对当代山水画家的影响,且对深受郭熙山水画影响的当代画家... 详细信息

笔者通过了解郭熙的生平背景,并对他的绘画风格和山水画绘画技法进行研究,包括郭熙用笔、用墨技法特点以及“三远法”的构图形式。阐述了郭熙《林泉高致》中提到的部分绘画理论对当代山水画家的影响,且对深受郭熙山水画影响的当代画家黄菲作品的分析,探讨了郭熙山水画风格和当代绘画的融合的方式及可行性。

关键词：郭熙山水画当代应用

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