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Activation of TLR-4 and liver injury via NF-kappa B in rat with acute cholangitis

Hepatobiliary & Pancreatic Diseases International 2008年第2期7卷 185-191页

作者： Yu, Hong wu, shuo-dong China Med Univ Dept Biliary & Minimal Invas Surg 1 Shengjing Hosp Shenyang 110004 Peoples R China

BACKGROUND: Toll-like receptors (TLRs) are a family of type 1 transmembrane receptors, which can recognize different pathogen-associated molecular patterns. Among them, TLR-4 is specific to lipopolysaccharide. It transfers the infection signal into the cell and promotes the translocation of nuclear factor kappa B (NF-kappa B) to the nucleus and the subsequent transcriptional activation of genes encoding pro- and anti-inflammatory cytokines and chemokines. Acute cholangitis (AC) is a common biliary tract infection in oriental countries, and often leads to liver injury. The activation of TLR-4 and its significance in liver injury in rats with AC remain unclear. METHODS: Rat models of AC (biliary tract obstruction+E. coli injection, n=36) and control models (biliary tract obstruction+saline, n=18) were made. Liver tissue injury was investigated by pathological examination. The levels of serum TNF-alpha and IL-10 were measured by enzyme-linked immunosorbent assay, and the expressions of TLR-4, NF-kappa B mRNAs and proteins in the liver were detected by RT-PCR, immunohistochemical staining and Western blotting, respectively. RESULTS: Severe liver tissue injury in rats with AC was evident as shown by pathological examination. TLR-4 and NF-kappa B were strongly expressed in the cytoplasm of hepatocytes in the AC group. They were negative or slightly positive in the control group. TLR-4 mRNA and protein in the liver of rats with AC increased 1 hour after biliary tract ligation and E. coli injection, and peaked at 6 hours after surgery. Twenty-four hours later, they began to decrease. The expression of TLR-4 was paralleled by that of NF-kappa B in the liver and TNF-alpha in serum. CONCLUSION: The higher expression of TLR-4 in the liver of rats with AC may be involved in liver injury through the activation of NF-kappa B and release of cytokines such as TNF-alpha.

关键词： acute cholangitis NF-kappa B toll-like receptor-4

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Effect of intestinal transit on the formation of cholesterol gallstones in hamsters

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Hepatobiliary & Pancreatic Diseases International 2007年第5期6卷 513-515页

作者： Fan, Ying wu, shuo-dong Fu, Bei-Bei China Med Univ Dept Gen Surg Affiliated Hosp Shengjing Hosp 2 Shenyang 110004 Peoples R China

BACKGROUND: The effect of 'intestinal transit' has become a new field of interest in the study of the pathogenesis of cholesterol gallstones. This study was undertaken to further test this notion and ascertain the relationship between impaired intestinal transit function and cholesterol gallstones. METHODS: A total of 64 hamsters were divided into 2 groups, experimental and control. Each was subdivided into 4 subgroups for sacrifice at different time. A high-cholesterol diet and a standard diet were fed to each group. The geometric center, which represents the intestinal transit function was calculated. RESULTS: The growth of all hamsters was normal. Cholesterol gallstones were found in 2 hamsters at the end of the 4th week. The geometric center values for the experimental and control groups were 2.3891 +/- 0.3923 vs. 2.7730 +/- 0.5283, at the end of week 3; 1.8148 +/- 0.4312 vs. 3.2294 +/- 1.1613 at week 4; 1.8451 +/- 0.3700 vs. 2.9075 +/- 0.3756 at week 5; and 1.8025 +/- 0.3413 vs. 3.0920 +/- 0.5622 at week 6. CONCLUSION: A high cholesterol diet can significantly reduce the intestinal transit function and facilitate the formation of cholesterol gallstones.

关键词： intestinal transit cholesterol gallstone

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Possible relationship between intestinal barrier function and formation of pigment gallstones in hamsters

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Hepatobiliary & Pancreatic Diseases International 2008年第5期7卷 529-532页

作者： Fan, Ying wu, shuo-dong Sun, Lei Fu, Bei-Bei Su, Yang China Med Univ Shengjing Hosp Dept Gen Surg Shenyang 110004 Peoples R China

BACKGROUND: The presence of bacteria in bile is an important factor in the formation of pigment gallstones. The bile of healthy people is sterile and bacteria in the biliary system come from endogenous infection from the gut. Yet, the route of bacterial translocation into the bile duct is still unclear. Theoretically, two routes exist: one is through the intestinal barrier and the other is by direct reflux from the sphincter of Oddi. This study was undertaken to explore the relationship between the effectiveness of intestinal barrier and the formation of pigment gallstones in hamsters. METHODS: Thirty-two hamsters were divided into an experimental and a control group, with 16 hamsters in each group. A low protein and high cellulose diet was given for 6 weeks to induce the formation of pigment gallstones in the experimental group (PS) and a normal diet was given to the control group (CON). Morphological changes, changes in the levels of serum endotoxin and diamine oxidase, and changes in the numbers of B lymphocytes, plasma cells and secretory immunoglobin A (sIgA) in the intestinal mucosa were assessed after 6 weeks. RESULTS: Four hamsters died during lithogenesis and body weight decreased in the PS group. Pigment gallstones were found in 11 hamsters at the end of the experiment, giving a lithogenesis rate of 91.67%. The serum endotoxin level before and after gallstone formation in the PS group was 0.2960 +/- 0.1734 U/ml and 8.2964 +/- 4.6268 U/ml, respectively (P<0.05). The blood diamine oxidase level before and after gallstone formation in the PS group was 2.6333 +/- 0.8037 U/ml and 3.3642 +/- 0.9545 U/ml, respectively (P<0.05). The numbers of B lymphocytes, plasma cells and sIgA in the intestinal mucosa in the PS group were 71.56 +/- 2.89, 68.65 +/- 2.09 and 27.56 +/- 1.07, respectively, and were significantly decreased compared with the corresponding values in the CON group (94.25 +/- 3.69, 93.47 +/- 3.98 and 42.57 +/- 1.96, respectively, P<0.05). CONCLUSIONS: A

关键词： endotoxins diamine oxidase B lymphocytes plasma cells secretory immunoglobin A

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Strengthening mechanism and forming control of linear friction welded GH4169 alloy joints

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Chinese Journal of Aeronautics 2024年第4期37卷 609-626页

作者： Yu SU Xiawei YANG Tingxi MENG Xinyuan HE dong wu Wenya LI shuo YIN State Key Laboratory of Solidification Processing School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Shaanxi Key Laboratory of Friction Welding Technologies School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Department of Mechanical Manufacturing and Biomedical EngineeringParsons BuildingTrinity College DublinThe University of DublinDublin 2Ireland

Numerical simulation and experimental research on Linear Friction Welding(LFW) for GH4169 superalloy were carried out. Based on the joint microstructure and mechanical properties,a suitable welding process was determined, which provided an important theoretical basis for the manufacture and repair of aeroengine components such as the superalloy blisk. The results show that the joint strain rate gradually increases with the increase of welding frequency, and the deformation resistance of the thermoplastic metal increases in the welding process, resulting in the interface thermoplastic metal not being extruded in time to form a flash, so the joint shortening amount gradually decreases. The thermoplastic metal in the center of the welding surface is kept at high welding temperature for a long time, resulting in the decrease of the joint strength. The microhardness of the joint shows a “W” distribution perpendicular to the weld, and most of the joints break in the Thermo-Mechanically Affected Zone(TMAZ) with high tensile strength and low *** the welding area is increased without changing the aspect ratio of the welding surface, the interface peak temperature increases gradually, and the joint shortening amount decreases with the increase of the welding interface size.

关键词： Linear Friction Welding(LFW) Microstructure Mechanical properties Numerical simulation Joint shortening amount

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Possible relationship between cholecystectomy and subsequent occurrence of primary common bile duct stones:a retrospective review of data

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Hepatobiliary & Pancreatic Diseases International 2007年第6期6卷 627-630页

作者： wu, shuo-dong Tian, Yu Kong, Jing Ding, Ren-Yu Jin, Jun-Zhe Guo, Ren-Xuan China Med Univ Shengjing Hosp Affiliated Hosp 2 Dept Gen Surg Shenyang 110004 Peoples R China China Med Univ Affiliated Hosp 1 Dept Gen Surg Shenyang 110001 Peoples R China

BACKGROUND: Most reports on the prognosis of cholecystectomy have been short-term studied, and few long-term reports have suggested variable incidences of common bile duct stones after cholecystectomy. We retrospectively reviewed the data to find the possible association between cholecystectomy and the subsequent occurrence of primary common bile duct stones. METHODS: The data were reviewed retrospectively of 478 patients with primary common bile duct stones diagnosed and treated by endoscopic sphincterotomy at our hospitals between January 1994 and December 2003. RESULTS: Sixty-one (14.1%) of the 432 patients had a history of cholecystectomy, with an incidence rate markedly higher than that in the general population. The mean interval between cholecystectomy and the occurrence of primary common bile duct stones was 8.23 years, with the longest being 28 years and the shortest 2 years. Compared with the patients who had not undergone a prior cholecystectomy, those who had had a prior cholecystectomy more often accompanied with acute cholangiolitis (chi(2)=8.259, P<0.01), and multiple stones or sand-like stones were frequently found (chi(2)= 9.030, P<0.01). CONCLUSIONS: These results suggest a possible relationship between cholecystectomy and the subsequent occurrence of primary common bile duct stones. Perhaps patients with primary common bile duct stones who have had a prior cholecystectomy have a higher probability of infection of the biliary system. The infection may be one of the causes of occurrence of primary common bile duct stones after cholecystectomy.

关键词： cholecystectomy common bile duct stones

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Benchmarking Algorithms for Gene Set Scoring of Single-cell ATAC-seq Data

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Genomics, Proteomics & Bioinformatics 2024年第2期22卷 27-42页

作者： Xi Wang Qiwei Lian Haoyu dong shuo Xu Yaru Su Xiaohui wu Pasteurien College Suzhou Medical College of Soochow UniversitySoochow UniversitySuzhou 215000China Department of Automation Xiamen UniversityXiamen 361005China College of Mathematics and Computer Science Fuzhou UniversityFuzhou 350116China

Gene set scoring(GSS)has been routinely conducted for gene expression analysis of bulk or single-cell RNA sequencing(RNA-seq)data,which helps to decipher single-cell heterogeneity and cell type-specific variability by incorporating prior knowledge from functional gene ***-cell assay for transposase accessible chromatin using sequencing(scATAC-seq)is a powerful technique for interrogating single-cell chromatin-based gene regulation,and genes or gene sets with dynamic regulatory potentials can be regarded as cell type-specific markers as if in single-cell RNA-seq(scRNA-seq).However,there are few GSS tools specifically designed for scATAC-seq,and the applicability and performance of RNA-seq GSS tools on scATAC-seq data remain to be ***,we systematically benchmarked ten GSS tools,including four bulk RNA-seq tools,five scRNA-seq tools,and one scATAC-seq ***,using matched scATAC-seq and scRNA-seq datasets,we found that the performance of GSS tools on scATAC-seq data was comparable to that on scRNA-seq,suggesting their applicability to ***,the performance of different GSS tools was extensively evaluated using up to ten scATAC-seq ***,we evaluated the impact of gene activity conversion,dropout imputation,and gene set collections on the results of *** show that dropout imputation can significantly promote the performance of almost all GSS tools,while the impact of gene activity conversion methods or gene set collections on GSS performance is more dependent on GSS tools or ***,we provided practical guidelines for choosing appropriate preprocessing methods and GSS tools in different application scenarios.

关键词： Single-cell ATAC-seq Gene set scoring Pathway analysis Single-cell RNA-seq Benchmark

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Relationship between intraduodenal peri-ampullary diverticulum and biliary disease in 178 patients undergoing ERCP

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Hepatobiliary & Pancreatic Diseases International 2007年第3期6卷 299-302页

作者： wu, shuo-dong Su, Yang Fan, Ying Zhang, Zhen-Hai Wang, Hao-Lin Kong, Jing Tian, Yu China Med Univ Dept Gen Surg Affiliated Hosp 2 Shenyang 110004 Peoples R China

BACKGROUND: The presence of intraduodenal periampullary diverticulum is often observed during upper digestive tract barium meal studies and endoscopic retrograde cholangiopancreatography (ERCP). A few papers in China and overseas reported that the diverticulum had something to do with the incidence of cholelithiasis. This study was undertaken to further test this notion and ascertain the relationship between intraduodenal periampullary diverticulum and biliary disease, especially the formation of bile duct pigment stones. METHODS: A total of 178 patients who had undergone ERCP or endoscopic sphincterotomy (EST) were studied retrospectively. They were divided into 6 groups according to the category of biliary disease, and the incidence rates of intraduodenal peri-ampullary diverticulum were calculated. duct pigment stones is higher than that in patients with bile duct stones secondary to gallbladder stones, patients with inflammation and stricture of the end of the bile duct and papilla, and patients with carcinoma of the end of the bile duct and papilla. These findings indicate that the anatomical abnormalities and malfunction of the sphincter of Oddi play an important role in the formation of bile duct pigment stones. RESULTS: There were 44 patients with intraduodenal periampullary diverticulum in 81 patients with primary bile duct pigment stones (54.32%), 4 in 8 patients with bile duct stones and gallbladder stones (50%), 7 in 33 patients with bile duct stones secondary to gallbladder stones (21.21%), 3 in 21 patients with inflammation and stricture of the end of the bile duct and papilla (14.29%), 1 in 22 patients with carcinoma of the end of the bile duct and papilla (4.54%), and 5 in 13 patients with post-cholecystectomy syndrome or sphincter of Oddi dysfunction (38.46%). CONCLUSIONS: The incidence rate of intraduodenal periampullary diverticulum in patients with primary bile duct pigment stones is higher than that in patients with bile duct stones secondary to

关键词： intraduodenal peri-ampullary diverticulum pigment stones endoscopic retrograde cholangiopancreatography sphincter of Oddi

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Differences and significance of motilin,vasoactive intestinal peptide and gastrin in blood and gallbladder tissues of patients with gallstones

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Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 58-64页

作者： Zhang, Zhen-Hai wu, shuo-dong Su, Yang Jin, Jun-Zhe Fan, Ying Yu, Hong Zhang, Li-Kui China Med Univ Affiliated Hosp 2 Dept Gen Surg Shenyang 110004 Peoples R China

BACKGROUND: The disorders of gallbladder motility may play an important role in the formation of gallstones. Many neural and hormonal factors and their interactions regulate gallbladder motility and bile flow into the duodenum. Further study in these factors may help to reveal the etiology of gallbladder diseases. This study was undertaken to assess the relationship of the levels of motilin, vasoactive intestinal peptide (VIP) and gastrin in blood and gallbladder tissues with the formation of cholelithiasis. METHODS: The levels of motilin, gastrin and VIP in blood and gallbladder tissues of 36 patients with gallbladder stones, 14 patients with gallbladder polyps, 10 healthy volunteers and 10 patients with common bile duct stones were measured by radioimmunoassay. RESULTS: The level of motilin in plasma and gallbladder tissues of the gallbladder stone group was higher than that of the control and gallbladder polyp groups (P<0.05). The levels of plasma VIP and serum gastrin were much higher than those of the other three groups (P<0.01). The level of VIP in gallbladder tissues was higher than that of the control and gallbladder polyp groups (P<0.01). CONCLUSIONS: The abnormal excretion of hormonal factors is closely related to gallstone formation. The high level of VIP in gallbladder tissues may be an important cause of gallbladder hypomotility. The abnormal level of serum gastrin may be related to the gastrointestinal symptoms of patients with gallstones.

关键词： motilin vasoactive intestinal peptide gastrin gallbladder radioimmunoassay

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Effect of linear friction welding process on microstructure evolution,mechanical properties and corrosion behavior of GH4169 superalloy

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Chinese Journal of Aeronautics 2024年第6期37卷 504-520页

作者： Yu SU Xiawei YANG Tingxi MENG dong wu Rui XU Haiyu XU Wenya LI shuo YIN State Key Laboratory of Solidification Processing School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Department of Mechanical Manufacturing&Biomedical EngineeringParsons BuildingTrinity College DublinThe University of DublinDublin 2Ireland

Linear Friction Welding(LFW)technology was used to realize the welding of GH4169 superalloy,and the effect of welding parameters on the microstructure,mechanical properties and corrosion behavior of the joint was *** results show that there is a positive correlation between the weld hardness and the tensile *** the gradual increase of heat input and welding pressure,the joint quality is gradually improved,but the heat affected zone is not significantly *** smaller the grain size of the weld,the higher the strength and plasticity of the *** the increase of the joint shortening amount,the corrosion resistance of the weld first gradually ***,when the shortening reaches a certain level,the corrosion resistance of the joint becomes little *** the increase of solution temperature,the corrosion current density increases and the polarization impedance *** higher the corrosion temperature,the worse the corrosion resistance of the *** is no significant correlation between the joint strength and the corrosion *** corrosion resistance of the joint can be enhanced without changing the joint mechanical properties by reducing the welding frequency and amplitude or increasing the welding pressure.

关键词： Linear Friction Welding(LFW) GH4169 superalloy Microstructure Mechanical properties Corrosion behavior

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Wettability Gradient-Induced Diode:MXene-Engineered Membrane for Passive-Evaporative Cooling

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Nano-Micro Letters 2024年第8期16卷 382-397页

作者： Leqi Lei shuo Meng Yifan Si shuo Shi Hanbai wu Jieqiong Yang Jinlian Hu Department of Biomedical Engineering City University of Hong KongHong Kong S.A.R 999077China

Thermoregulatory textiles,leveraging high-emissivity structural materials,have arisen as a promising candidate for personal cooling management;however,their advancement has been hindered by the underperformed water moisture transportation capacity,which impacts on their thermophysiological ***,we designed a wettability-gradient-induced-diode(WGID)membrane achieving by MXene-engineered electrospun technology,which could facilitate heat dissipation and moisture-wicking *** a result,the obtained WGID membrane could obtain a cooling temperature of 1.5℃ in the“dry”state,and 7.1℃ in the“wet”state,which was ascribed to its high emissivity of 96.40%in the MIR range,superior thermal conductivity of 0.3349 W m^(-1) K^(-1)(based on radiation-and conduction-controlled mechanisms),and unidirectional moisture transportation *** proposed design offers an approach for meticulously engineering electrospun membranes with enhanced heat dissipation and moisture transportation,thereby paving the way for developing more efficient and comfortable thermoregulatory textiles in a high-humidity microenvironment.

关键词： Passive-evaporative cooling MXene Electrospun membrane Wettability gradient Diode

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