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Combination of Quercetin, Cinnamaldehyde and Hirudin Protects Rat Dorsal Root Ganglion Neurons against High Glucose-Induced Injury through Nrf-2/HO-1 Activation and NF-κB Inhibition

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Chinese Journal of Integrative Medicine 2017年第9期23卷 663-671页

作者： SHI Yue liANG Xiao-chun ZHANG Hong SUN Qing wu qun-li QU ling Department of Traditional Chinese Medicine Translational Medicine Center Peking Union Medical College HospitalChinese Academy of Medical Sciences Department of Pathology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College

Objective： TO examine the effects of the combination of quercetin （Q）, cinnamaldehyde （C） and hirudin （H）, a Chinese medicine formula on high glucose （HG）-induced apoptosis of cultured dorsal root ganglion （DRG） neurons. Methods： DRG neurons exposed to HG （45 mmol/L） for 24 h were employed as an in vitro model of diabetic neuropathy. Cell viability, reactive oxygen species （ROS） level and apoptosis were determined. The expression of nuclear factor of Kappa B （NF- κB）, inhibitory kappa B α （IκBα ）, phosphorylated IκBα and Nf-E2 related factor 2 （Nrf2） were examined using reverse transcription-polymerase chain reaction （RT-PCR） and Western blot assay. The expression of hemeoxygenase-1 （HO-1）, interleukin-6 （IL-6）, tumor necrosis factor （TNF-α） and caspase-3 were also examined by RT-PCR and Western blot assay. Results： HG treatment markedly increased DRG neuron apoptosis via increasing intracellular ROS level and activating the NF- κB signaling pathway （P〈0.05）. Co-treatment with Q, C, H and their combination decreased HG-induced caspase-3 activation and apoptosis （P〈0.05 or P〈0.01）. The expressions of NF- κB, IL-6 and TNF-α were down- regulated, and Nrf2/HO-1 expression was up-regulated （P〈0.05 or P〈0.01）. QCH has better effect in scavenging ROS, activating Nrf-2/HO-1, and down-regulating the NF- κB pathway than other treatment group. Conclusions： DRG neurons＇ apoptosis was increased in diabetic conditions, which was reduced by QCH formula treatment. The possible reason could be activating Nrf-2/HO-1 pathway, scavenging ROS, and inhibition of NF- κB activation. The effect of QCH combination was better than each monomer or the combination of the two monomers.

关键词： diabetic peripheral neuropathy oxidative stress apoptosis dorsal root ganglion neurons Chinese herb

Expression of transforming growth factors in hepatocellular carcinoma and its relations with clinicopathological parameters and prognosis

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Hepatobiliary & Pancreatic Diseases International 2008年第2期7卷 174-178页

作者： Lu, Yun wu, li-qun li, Chang-Sheng Wang, Shou-Guang Han, Bing Qingdao Univ Dept Hepatobiliary Surg Affiliated Hosp Coll Med Qingdao 266003 Peoples R China

BACKGROUND: Transforming growth factors (TGF)-beta 1, TGF-beta R2 and Smad4 belong to the TGF family, and play important roles in carcinogenesis and the development of carcinoma, especially hepatocellular carcinoma (HCC). TGF-beta 1 is a multipotent polypeptide, which inhibits the growth of epithelial cells including hepatoma cell lines and hepatocytes by inducing apoptosis. TGF-beta R2 forms a heterodimeric complex upon binding to TGF-beta, and then generates the first step in the signal transduction pathway leading to growth inhibition in coordination with the type I receptor. Smad4 protein is an important mediator in the TGF-beta signaling pathway, and negatively regulates the growth of epithelial cells. This study aimed to detect the expression of TGF-beta 1, TGF-beta R2 and Smad4 in HCCs and their adjacent normal tissues, while assessing its relations with the clinicopathological parameters of HCC. METHODS: Forty-seven HCC specimens and their adjacent normal tissues were obtained surgically at the Affiliated Hospital of Medical College, Qingdao University. The expression of TGF-beta 1, TGF-beta R2 and Smad4 was separately detected by immunohistochemistry in all HCC specimens and their adjacent normal tissues, and its relations with the clinicopathological parameters of HCC were assessed. RESULTS: The positive expression of TGF-beta 1 was 72.34% in the HCC specimens, which was higher than that in the adjacent normal tissues (P < 0.001). The positive expression of Smad4 and TGF-beta R2 was 34.04% and 59.57% respectively in the carcinoma specimens. The expression of TGF-beta 1, TGF-beta R2 and Smad4 was significantly higher in groups with a tumor embolus of the portal vein, integrity of the amicula, and Edmondson's III-IV than that in other groups, but it was not related to tumor size (P < 0.05). CONCLUSIONS: TGF-beta 1 may play an important role in the occurrence and development of HCC. Combined detection of TGF-beta 1, TGF-beta R2 and Smad4 may be useful for the

关键词： carcinoma hepatocellular transforming growth factor immunohistochemistry

Predominant expression of Th1-type cytokines in primary hepatic cancer and adjacent liver tissues

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Hepatobiliary & Pancreatic Diseases International 2007年第1期6卷 63-66页

作者： Qiu, Fa-Bo wu, li-qun Lu, Yun Zhang, Shun Zhang, Bing-Yuan Qingdao Univ Affiliated Hosp Dept Hepatobiliary Vasc Surg Qingdao 266003 Peoples R China

BACKGROUND: Research has revealed a shift towards Th2 in many types of malignant tumor, but the state of Th1/Th2 is not clear in patients with primary hepatic cancer (PHC). This study was designed to determine the expression of Th1- versus Th2-type cytokines in primary hepatic cancer and the adjacent liver tissue in order to provide evidence for treatment of the Th1/Th2 shift. METHODS: Samples were collected from 11 patients with PHC. The gene expression of Th1/Th2 cytokines was detected by reverse transcriptase polymerase chain reaction (RT-PCR) using IFN-gamma and IL-2 as Th1-type cytokine genes, and IL-4 and IL-10 as Th2-type cytokine genes. RESULTS: Th1-type cytokines were expressed in 7/11 PHCs and 9/11 adjacent liver tissues, while Th0 type cytokines occurred in 4/11 PHCs and 2/11 adjacent liver tissues. CONCLUSION: Th1-type cytokines are expressed predominantly in primary hepatic cancer and the adjacent liver tissue.

关键词： primary hepatic carcinoma cytokine gene expression reverse transcriptase polymerase chain reaction

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我国全要素生产率变化解构及区域收敛性分析

经济经纬 2010年第1期27卷 12-16页

作者：武群丽华北电力大学经济管理系河北保定071003

笔者利用Malmquist生产力指数将199t年～2007年间中国29个省（自治区、直辖市）的全要素增长率按照构成分解为技术变动和技术效率变动，并对区域技术效率的收敛性进行了分析。结果表明：（1）以1997年为界限，东部、中部和西部三大区域... 详细信息

笔者利用Malmquist生产力指数将199t年～2007年间中国29个省（自治区、直辖市）的全要素增长率按照构成分解为技术变动和技术效率变动，并对区域技术效率的收敛性进行了分析。结果表明：（1）以1997年为界限，东部、中部和西部三大区域的全要素生产率和技术变动均呈现先升后降的趋势，而技术效率在整个研究期间都是逐步降低的。（2）技术变动解释了全要素增长率区域差异的75％，技术效率变动则解释了剩余的25％。1997年之前的全要素生产率及其构成因素的变化有利于缩小地区生产差距，1997年之后则成为区域差距扩大的重要原因之一。（3）29个省份之间以及三大区域内部各省份之间的技术效率随时间变化均呈现出明显的发散趋势。

关键词：全要素生产率变动技术变动技术效率变动收敛性

Expression of SSX-1 and NY-ESO-1 mRNA in tumor tissues and its corresponding peripheral blood expression in patients with hepatocellular carcinoma

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Chinese Medical Journal 2007年第12期120卷 1042-1046页

作者： LU Yun wu li-qun LU Zheng-hua WANG Xin-jian YANG Jing-yang Department of Hepatobiliary Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China Center of Cellular and Molecular Pathology Affiliated Hospital of Medical College Qingdao University Qingdao 266003 China

Background Cancer-testis antigen （CTA） is a family of the most noticeable tumor antigens which could be potential tumor markers for cancer diagnosis. In this research we aimed to investigate the expression of SSX-1 and NY-ESO-1 mRNA, two members of the CTA family, in tissue and peripheral blood of patients with hepatocellular carcinoma （HCC） to assess their feasibility for the immunotherapy and diagnosis of HCC and the association of their expression levels with diverse clinical indicators. Methods Thirty-six north Chinese patients with HCC and 30 normal controls were enrolled in this study. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of SSX-1 and NY-ESO-1 mRNA in tumor tissues and corresponding levels in peripheral blood of patients. Results The positive rates of SSX-1 and NY-ESO-1 mRNA expression were 61.1% （22/36） and 11.1% （4/36）, respectively, in cancer tissues; 38.9% （14/36） and 5.6% （2/36）, respectively, in the corresponding peripheral blood samples. No positive expression of either SSX-1 or NY-ESO-1 mRNA was detected in the samples of cancer-adjacent tissues, cirrhotic tissues, normal liver tissue or the peripheral blood of control patients. No significant relationship was found between the expression of these two genes and clinical indicators such as age, gender, tumor size, extent of differentiation, serum a-fetoprotein （AFP） level or infection with hepatitis B virus （P〉0.05）. The short term recurrence rate was 46.2% （6/13） in patients whose peripheral blood expressed SSX-1 mRNA, while the recurrence rate in patients with negative SSX-1 mRNA was 28.6% （4/14）. Conclusions SSX-1 and NY-ESO-1 antigens might be new potentially promising targets for antigen-specific immunotherapy for HCC. High specific expression of SSX-1 and NY-ESO-1 mRNA suggested that we could apply them as tumor markers. The short term recurrence rate was significantly higher in patients whose peripheral blood expressed SSX-1 mRNA, sugges

关键词： cancer-testis antigen hepatocellular carcinoma reverse transcription-polymerase chain reaction SSX-1 NY-ESO-1

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利益集团影响下的政府管制决策行为分析

经济经纬 2005年第3期22卷 10-12页

作者：武群丽华北电力大学经济管理学院河北保定071003

管制政策的形成是政策需求和政策供给两种力量相互作用的结果,当社会各利益集团处于某一初始资源配置点时,追求公众利益最大化的政府采取什么样的管制政策,取决于该初始禀赋下利益集团的公平效用及管制所造成的净损益效用。

关键词：管制利益集团政府效用

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基于数据挖掘技术的桥梁结构健康状态检测

吉林大学学报（工学版） 2020年第2期50卷 565-571页

作者：武立群张亮亮西南大学工程技术学院重庆400715 重庆大学土木工程学院重庆400045

针对当前桥梁结构健康状态检测模型存在的检测误差大、检测效率差等难题,以改善桥梁结构健康状态检测结果为目标,设计了基于数据挖掘技术的桥梁结构健康状态检测模型。首先,采用无线传感器网络对桥梁结构健康状态数据进行采集,并采用核... 详细信息

针对当前桥梁结构健康状态检测模型存在的检测误差大、检测效率差等难题,以改善桥梁结构健康状态检测结果为目标,设计了基于数据挖掘技术的桥梁结构健康状态检测模型。首先,采用无线传感器网络对桥梁结构健康状态数据进行采集,并采用核主成分分析对桥梁结构健康数据处理,去除桥梁结构健康数据的冗余特征,减少桥梁结构健康状态检测特征规模;然后,采用支持向量机对桥梁结构健康数据进行学习,并引入粒子群优化算法确定的桥梁结构健康状态检测模型的参数,建立最优的桥梁结构健康状态检测模型;最后,在Matlab2017平台上对桥梁结构健康模型的有效性和优越性进行测试。结果表明:本文模型获得了较高精度的桥梁结构健康状态检测结果,桥梁结构健康建模时间减少,提高了桥梁结构健康状态检测效率,而且桥梁结构健康状态检测整体性能要明显优于当前其他的桥梁结构健康检测模型,为桥梁结构健康研究提供了一种有效的工具。

关键词：桥梁结构健康状态核主成分分析冗余特性检测精度建模效率

Efficacy and Safety of Mulberry Twig Alkaloids Tablet for Treatment of Type 2 Diabetes:A Randomized,Double-Blind,Placebo-Controlled Multicenter Clinical Study

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Chinese Journal of Integrative Medicine 2022年第4期28卷 304-311页

作者： QU ling liANG Xiao-chun TIAN Guo-qing ZHANG Gai-li wu qun-li HUANG Xiu-mei CUI Ya-zhong liU Yu-ling SHEN Zhu-fang MA Guo-qing LU Hao li Yi JIANG Hong YANG Xi-yan ZHANG Guang-de YANG Chen-hua Department of Traditional Chinese Medicine Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China Department of Endocrinology the Second Affiliated Hospital of Heilongjiang University of Chinese MedicineHaerbin150001China Department of Endocrinology Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghai201203China Department of Traditional Chinese Medicine Beijing HospitalBeijing10005China Department of Endocrinology the Second Affiliated Hospital of Liaoning University of Traditional Chinese MedicineShenyang110034China Department of Geriatrics the First Affiliated Hospital of Tianjin University of Chinese MedicineTianjin300073China Department of Endocrinology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing100091China Department of Endocrinology Affiliated Hospital of Henan Academy of Traditional Chinese MedicineZhengzhou450003China

Objective:To assess the efficacy and safety of mulberry twig alkaloids(Sangzhi alkaloids,SZ-A)for treatment of type 2 diabetes in a randomized,double-blind,placebo-controlled multicenter clinical ***:A total of 200 patients were randomized to receive SZ-A(n=100)or placebo(n=100)for 16 *** data analysis system for electronic data capture clinical trial central randomization system was used for randomization and dispensing of *** primary outcome was the change in glycosylated hemoglobin(Hb A1c)*** secondary outcome included the proportions of cases with Hb A1cliver and kidney function were ***:Compared with baseline,the change of Hb A1c at week 16 was-0.80%(95%CI:-0.98%to-0.62%)and-0.09%(95%CI:-0.27%to 0.09%)in SZ-A group and placebo group,*** proportion of patients with Hb A1c0.05).The incidence rates of AEs,TAEs,and GDs differed significantly between groups(P=0.010,P=0.005,and P=0.006,respectively),whereas the incidence rates of SAEs showed no significant differences between groups(P=1.000).Conclusion:SZ-A are effective and safe for treatment of type 2 diabetes.

关键词： type 2 diabetes mellitus mulberry twig alkaloids tablets(Sangzhi alkaloids) efficacy safety placebo

Optimization of atrioventricular delay by surface electrocardiography during dual chamber pacing

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Chinese Medical Journal 2006年第6期119卷 454-457页

作者： wu li-qun GU Gang CAO Min SHEN Yong-chu SU Kan SHEN Wei-feng Department of Cardiology Ruijin Hospital Shanghai Jiao TongUniversity Shanghai 200025 China

Background Nominal atrioventricular （AV） interval in dual chamber pacemaker （DDD） is not the best AV delay in the majority of patients with atrioventricular block. To find a simple method for optimizing AV delay adjustment, we assessed surface electrocardiography （ECG） for optimizing AV delay during dual chamber pacing. Methods DDD pacemakers were implanted in 46 patients with complete, or almost complete, AV block. Optimal AV delay was achieved by programming an additional delay of 100 ms, to the width of intrinsic P wave or to the interval between pacing spike to the end of P wave on surface ECG. Leit ventricular （LV） end diastolic and end systolic volumes, ejection fraction and diastolic parameters were measured by Doppler echocardiography during both nominal and optimal AV delay pacing.Results Compared to nominal AV delay setting, LV end diastolic volume increased [to （53.2±11.3） ml from （50.2 ± 10.2） ml, P〈0.05], end systolic volume decreased [to （26.1 ± 9.0） ml from （27.9 ± 8.2） ml, P〈0.05] during adjusted AV delay pacing, resulting in an increase in LV ejection fraction [to （68.2±5.3）% from （64.5±4.3）%, P〈0.05]. LV diastolic filling and isovolumic relaxation time were not significantly changed.Conclusion Optimization of AV delay by surface ECG is a simple method to improve LV systolic function during dual chamber pacing.

关键词： atrioventricular delay electrocardiography pacing P-wave