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Dynamic expression of rat heat shock protein gp96 and its gene during development of hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2007年第6期6卷 616-621页

作者： wu, xin-hua Yao, Deng-Fu Su, Xiao-Qin Tai, Bo-Jun huang, hua Qiu, Li-Wei wu, Wei Shao, Yi-Xiang Nantong Univ Affiliated Hosp Res Ctr Clin Mol Biol Nantong 226001 Peoples R China Nantong Univ Affiliated Hosp Dept Infect Dis Nantong Peoples R China Nantong Univ Affiliated Hosp Dept Pathol Nantong Peoples R China Nantong Univ Ctr Med Lab Anim Nantong 226001 Peoples R China

BACKGROUND: Hepatocellular carcinoma (HCC) is characterized by multicause, obvious multistage and multifocal processes of tumor progression. The development of HCC is related intimately to overexpression and signal transduction of many cellular factors. This study was undertaken to investigate the dynamic expression and alteration of heat shock protein (HSP) gp96 along with its gene during HCC development. METHODS: A rat model of hepatoma induced with 2-fluorenylacetamide (2-FAA, 0.05%) was established in male Sprague-Dawley rats. Total RNA and pathological changes were observed during hepatocarcinogenesis. Total RNAs were transcribed into cDNA by reverse transcription and the gene fragment of gp96 was amplified by nested RT-PCR. The gp96 expression in rat liver tissues was semi-quantitatively analyzed by immunohistochemistry. RESULTS: Histological examination suggested that hepatocytes in rats fed with 2-FAA showed vacuole-like denaturation at the early stages, then dysplastic nodules appeared at the middle stage, and finally progressed to tubercles of cancerous nests. A tendency of increasing liver gp96 protein level was found from normal liver to precancerous to cancerous tissues during hepatoma development (P<0.01), and was in accordance with the changes in gp96 mRNA (P<0.05). CONCLUSION: HSP gp96 is involved in HCC development and its overexpression may be a useful marker for early diagnosis.

关键词： hepatocellular carcinoma HSP gp96 RT-PCR immunohistochemistry

Expression and alterations of different molecular form γ-glutamyl transferase and total RNA concentration during the carcinogenesis of rat hepatoma

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World Journal of Gastroenterology 1999年第4期5卷 84-86页

作者： TANG Qi Yun, YAO Deng Fu, LU Jian xin, wu xin hua and MENG Xian Yong

ＩＮＴＲＯＤＵＣＴＩＯＮＣａｒｃｉｎｏｇｅｎｅｓｉｓｉｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｗｉｔｈＤＮＡｓｙｎｔｈｅｓｉｓａｎｄｈｙｐｅｒｍｅｔａｂｏｌｉｓｍｏｆｎｕｃｌｅｉｃａｃｉｄ．γｇｌｕｔａｍｙｌｔｒａｎｓｆｅｒ... 详细信息

ＩＮＴＲＯＤＵＣＴＩＯＮＣａｒｃｉｎｏｇｅｎｅｓｉｓｉｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｗｉｔｈＤＮＡｓｙｎｔｈｅｓｉｓａｎｄｈｙｐｅｒｍｅｔａｂｏｌｉｓｍｏｆｎｕｃｌｅｉｃａｃｉｄ．γｇｌｕｔａｍｙｌｔｒａｎｓｆｅｒａｓｅ（ＧＧＴ，ＥＣ２．３．２．２...

关键词： liver neoplasms/enzymology γ glutamyl transferase/analysis disease models, animal RNA 2 fluoenyacetamide/analysis

Characteristics of hepatic nuclear-transcription factor-kappa B expression and quantitative analysis in rat hepatocarcinogenesis

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Hepatobiliary & Pancreatic Diseases International 2009年第5期8卷 504-509页

作者： wu, Wei Yao, Deng-Fu Qiu, Li-Wei Sai, Wen-Li Shen, Jun-Jun Yu, Hong-Bo wu, xin-hua Li, Yue-Ming Wang, Yi-Lang Gu, Wen-Jing Nantong Univ Affiliated Hosp Res Ctr Clin Mol Biol Nantong 226001 Peoples R China

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. We analyzed the expression of miclear-transcription factor-kappa B (NF-kappa B) during hepatocarcinogenesis in order to evaluate its dynamic expression and its clinical value in the development and diagnosis of HCC. METHODS: Hepatoma models were induced by oral administration of 2-acetamidoflurene (2-FAA) to male Sprague-Dawley rats. Morphological changes were observed after hematoxylin and eosin staining. The cellular distribution of NF-kappa B expression during different stages of cancer development was investigated by immunohistochemistry, and the level of NF-kappa B expression in liver tissues was quantitatively analyzed by ELISA. The gene fragments of hepatic NF-kappa B were amplified by nested-polymerase chain reaction assay. RESULTS: Hepatocytes showed vacuole-like degeneration during the early stages, then had a hyperplastic nodal appearance during the middle stages, and finally progressed to tubercles of cancerous nests with high differentiation. The NF-kappa B-positive material was buff-colored, fine particles localized in the nucleus, and the incidence of NF-kappa B-positive cells was 81.8% in degeneration, 83.3% in precancerous lesions, and 100% in cancerous tissues. All of these values were higher than those in controls (P<0.01). Hepatic NF-kappa B expression and hepatic NF-kappa B-mRNA were also higher during the course of HCC development (P<0.01). CONCLUSION: The NF-kappa B signal transduction pathway is activated during the early stages of HCC development, and its abnormal expression may be associated with the occurrence of HCC.

关键词： hepatocellular carcinoma nuclear factor-kappa B immunohistochemistry nested-polymerase chain reaction NF-kappa B-mRNA

Resting-state brain network remodeling after different nerve reconstruction surgeries:a functional magnetic resonance imaging study in brachial plexus injury rats

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Neural Regeneration Research 2025年第5期20卷 1495-1504页

作者： Yunting Xiang Xiangxin xing Xuyun hua Yuwen Zhang xin Xue Jiajia wu Mouxiong Zheng He Wang Jianguang Xu School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina Department of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Traumatology and Orthopedics Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence(Fudan University) Ministry of EducationShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Distinct brain remodeling has been found after different nerve reconstruction strategies,including motor representation of the affected ***,differences among reconstruction strategies at the brain network level have not been *** study aimed to explore intranetwork changes related to altered peripheral neural pathways after different nerve reconstruction surgeries,including nerve repair,endto-end nerve transfer,and end-to-side nerve ***–Dawley rats underwent complete left brachial plexus transection and were divided into four equal groups of eight:no nerve repair,grafted nerve repair,phrenic nerve end-to-end transfer,and end-to-side transfer with a graft sutured to the anterior upper ***-state brain functional magnetic resonance imaging was obtained 7 months after *** independent component analysis algorithm was utilized to identify group-level network components of interest and extract resting-state functional connectivity values of each voxel within the *** in intra-network resting-state functional connectivity were compared among the *** muscle reinnervation was assessed by behavioral observation(elbow flexion)and *** results showed that alterations in the sensorimotor and interoception networks were mostly related to changes in the peripheral neural *** repair was related to enhanced connectivity within the sensorimotor network,while end-to-side nerve transfer might be more beneficial for restoring control over the affected limb by the original motor *** thalamic-cortical pathway was enhanced within the interoception network after nerve repair and end-to-end nerve *** areas related to cognition and emotion were enhanced after end-to-side nerve *** study revealed important brain networks related to different nerve *** networks may be potential targets for enhancing motor recovery.

关键词： brain functional networks end-to-end nerve transfer end-to-side nerve transfer independent component analysis nerve repair peripheral plexus injury resting-state functional connectivity

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论公共领导创新

中国行政管理 2006年第6期 23-25页

作者：吴新华北京大学政府管理学院北京100871

公共领导创新是国家创新体系的核心内容。公共领导创新的重要战略作用,决定了公共领导必须进行自主创新,创新领导理念、领导角色和领导功能,进而引导、带动和服务被领导者创新,推动创新型国家的建设。

关键词：公共领导领导创新领导理念领导角色领导功能

PPP模式在少数民族旅游项目开发中的应用研究

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贵州民族研究 2016年第9期37卷 180-183页

作者：吴新华山东科技大学资源与土木工程系山东泰安271019 山东科技大学采矿工程学院山东青岛266590

少数民族旅游项目由于具有风险高、投资周期长、资本回收周期长、投资规模大等特征,其传统投资模式往往是以政府为主导,政府通过拨付财政资金进行少数民族旅游项目的开发。然而这种单一的投资主体往往具有很多弊端,如无法调动社会资本... 详细信息

少数民族旅游项目由于具有风险高、投资周期长、资本回收周期长、投资规模大等特征,其传统投资模式往往是以政府为主导,政府通过拨付财政资金进行少数民族旅游项目的开发。然而这种单一的投资主体往往具有很多弊端,如无法调动社会资本、单一主体带来的风险集中、无法利用外部先进管理经验等。基于此"PPP模式"将能化解这些问题,本文系统分析PPP模式运用于少数民族旅游项目开发的必要性、可行性以及具体运用流程,并给出相关的应用策略和建议。

关键词： PPP模式少数民族旅游项目开发应用

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基于混沌的位级图像加密算法研究

微电子学与计算机 2013年第12期30卷 69-72页

作者：吴新华南通纺织职业技术学院现教中心江苏南通226007

提出了一种新的位级图像混沌加密算法.首先将平文图像划分为8幅位级图像并将其作为一个整体,利用Chen′s混沌系统生成混沌序列,进行位级图像置乱,然后利用Logistic混沌序列进行图像像素扩散,最后进行测试仿真和性能分析,并与其他图像加... 详细信息

提出了一种新的位级图像混沌加密算法.首先将平文图像划分为8幅位级图像并将其作为一个整体,利用Chen′s混沌系统生成混沌序列,进行位级图像置乱,然后利用Logistic混沌序列进行图像像素扩散,最后进行测试仿真和性能分析,并与其他图像加密算法进行比较,测试结果表明所提算法具有较强的安全性和有效性.

关键词：混沌图像加密位级图像置乱扩散

空气在不同高度波纹流道内的流阻与传热的数值分析

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哈尔滨工程大学学报 2007年第10期28卷 1100-1103页

作者：吴华新孙刚姜任秋哈尔滨工程大学建筑工程学院黑龙江哈尔滨150001 哈尔滨工程大学动力与能源工程学院黑龙江哈尔滨150001

空气-水波纹板式加热器采用不同于传统的肋片管式加热器的设计方法,传热面为一次表面,与传热介质接触的表面直接参与传热过程,具有结构紧凑、高效的特点,可将其用于空调机组加热器、热风幕等场合.利用流体力学软件FLUENT对空气侧的不同... 详细信息

空气-水波纹板式加热器采用不同于传统的肋片管式加热器的设计方法,传热面为一次表面,与传热介质接触的表面直接参与传热过程,具有结构紧凑、高效的特点,可将其用于空调机组加热器、热风幕等场合.利用流体力学软件FLUENT对空气侧的不同高度波纹流道内的流阻与传热过程进行模拟计算,通过比较表面摩擦系数f与传热因子j,表明波纹高度是影响空气在波纹流道内的流阻与传热过程的重要因素,结果可为进一步优化设计空气-水波纹板式加热器提供重要参考.

关键词：空气-水波纹板加热器流阻传热数值特性分析

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轻-重导向浮阀组合塔板的雾沫夹带实验研究

化学工程 2020年第10期48卷 34-39页

作者：吴昕华赵培黄婕华东理工大学化工学院上海200237

以空气-水为系统,在直径1000 mm、内置4块塔板的冷模塔中,对轻-重导向浮阀组合塔板进行了雾沫夹带实验的研究,在开孔率为15.25%条件下:就塔板结构改变了4种轻-重导向浮阀组合方式、3种堰高hw;就操作参数改变了3种液流强度L,分别测定了... 详细信息

以空气-水为系统,在直径1000 mm、内置4块塔板的冷模塔中,对轻-重导向浮阀组合塔板进行了雾沫夹带实验的研究,在开孔率为15.25%条件下:就塔板结构改变了4种轻-重导向浮阀组合方式、3种堰高hw;就操作参数改变了3种液流强度L,分别测定了雾沫夹带率ev随空塔动能因子F0变化的关系曲线。实验结果表明,轻-重导向浮阀组合塔板的雾沫夹带性能良好,且对液流强度变化最为敏感。其中重阀配比为20%的1#板,其雾沫夹带率较全轻阀减少约15%,并由实验数据拟合了该板的雾沫夹带率模型关联式。同时标绘了4种重阀比塔板的雾沫夹带液泛曲线图,表明1#板的操作弹性最好,对工业装置设计具有一定的指导意义。

关键词：轻-重导向浮阀组合塔板雾沫夹带率重阀比空塔动能因子