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Polarization-sensitive intensity diffraction tomography

Light(Science & Applications) 2023年第6期12卷 1071-1083页

作者： Seungri Song Jeongsoo kim Taegyun Moon Baekcheon Seong woovin kim Chang-Hyuk Yoo Jun-Kyu Choi Chulmin Joo Department of Mechanical Engineering Yonsei UniversitySeoul 03722Republic of Korea Small Machines Company Ltd.Seoul 04808Republic of Korea

Optical anisotropy,which is an intrinsic property of many materials,originates from the structural arrangement of molecular structures,and to date,various polarization-sensitive imaging(PSI)methods have been developed to investigate the nature of anisotropic *** particular,the recently developed tomographic PSI technologies enable the investigation of anisotropic materials through volumetric mappings of the anisotropy distribution of these ***,these reported methods mostly operate on a single scattering model,and are thus not suitable for three-dimensional(3D)PSI imaging of multiple scattering ***,we present a novel reference-free 3D polarization-sensitive computational imaging technique—polarization-sensitive intensity diffraction tomography(PS-IDT)—that enables the reconstruction of 3D anisotropy distribution of both weakly and multiple scattering specimens from multiple intensity-only measurements.A 3D anisotropic object is illuminated by circularly polarized plane waves at various illumination angles to encode the isotropic and anisotropic structural information into 2D intensity *** information are then recorded separately through two orthogonal analyzer states,and a 3D Jones matrix is iteratively reconstructed based on the vectorial multi-slice beam propagation model and gradient descent *** demonstrate the 3D anisotropy imaging capabilities of PS-IDT by presenting 3D anisotropy maps of various samples,including potato starch granules and tardigrade.

关键词： materials anisotropy intensity

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E2E-BPF microscope:extended depth-of-field microscopy using learning-based implementation of binary phase filter and image deconvolution

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Light(Science & Applications) 2023年第12期12卷 2610-2623页

作者： Baekcheon Seong woovin kim Younghun kim Kyung-A Hyun Hyo-Il Jung Jong-Seok Lee Jeonghoon Yoo Chulmin Joo Department of Mechanical Engineering Yonsei UniversitySeoul 03722Republic of Korea The DABOM Inc Seoul 03722Republic of Korea School of Integrated Technology Yonsei UniversityIncheon 21983Republic of Korea

Several image-based biomedical diagnoses require high-resolution imaging capabilities at large spatial ***,conventional microscopes exhibit an inherent trade-off between depth-of-field(DoF)and spatial resolution,and thus require objects to be refocused at each lateral location,which is time ***,we present a computational imaging platform,termed E2E-BPF microscope,which enables large-area,high-resolution imaging of large-scale objects without serial *** method involves a physics-incorporated,deep-learned design of binary phase filter(BPF)and jointly optimized deconvolution neural network,which altogether produces high-resolution,high-contrast images over extended depth *** demonstrate the method through numerical simulations and experiments with fluorescently labeled beads,cells and tissue section,and present high-resolution imaging capability over a 15.5-fold larger DoF than the conventional *** method provides highly effective and scalable strategy for DoF-extended optical imaging system,and is expected to find numerous applications in rapid image-based diagnosis,optical vision,and metrology.

关键词： image diagnosis binary

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