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The Quadrennial Ozone Symposium 2016

Advances in Atmospheric Sciences 2017年第3期34卷 283-288页

作者： Sophie GODIN-BEEKMANN Irina PETROPAVLOSKIKH Stefan REIS Paul NEWMAN wolfgang steinbrecht Markus REX Michelle L. SANTEE Richard S. ECKMAN Xiandong ZHENG Matthew B. TULLY David S. STEVENSON Paul YOUNG John PYLE Mark WEBER Johanna TAMMINEN Gina MILLS Alkis F. BAIS Clare HEAVISIDE Christos ZEREFOS Observatoire de Versailles Saint-Quentin en Yvelines Universite de Versailles Saint-Quentin-en-Yvelines CNRS 78280 Guyancourt France CIRES University of Colorado Boulder CO 80309 USA NERC Centre for Ecology & Hydrology Edinburgh EH26 OQB UK Goddard Space Flight Center NASA Greenbelt MD 20771 USA Hohenpeissenberg Meteorological Observatory Deutscher Wetterdienst 82383 Hohenpeissenberg Germany Alfred Wegener Institute 14401 Potsdam Germany Jet Propulsion Laboratory California Institute of Technology CA 91109 USA NASA Headquarters Earth Science Division Washington DC USA Chinese Academy of Meteorological Sciences Beijing 100081 China Bureau of Meteorology Melbourne Victoria 3001 Australia University of Edinburgh School of GeoSciences Edinburgh EH9 3FE UK Lancaster Environment Centre Lancaster University Lancaster LA1 4YQ UK University of Cambridge Department of Chemistry Cambridge CB2 1EW UK University of Bremen Institute of Environmental Physics 28359 Bremen Germany Finnish Meteorological Institute Earth Observation FI-00101 Helsinki Finland NERC Centre for Ecology and Hydrology Bangor Gwynedd LL57 2UW Wales UK Aristotle University of Thessaloniki ELKE AUTH Thessaloniki Greece Public Health England Centre for Radiation Chemical and Environmental Hazards London UK Research Center for Atmospheric Physics & Climatology Academy of Athens Athens 10680 Greece University of Exeter Medical School Truro TR1 3HD UK

1. Overview The 2016 Quadrennial Ozone Symposium （QOS-2016） was held on 4-9 September 2016 in Edinburgh, UK. The Symposium was organized by the International Ozone Commission （IO3C）, the NERC Centre for Ecology ＆ Hydrology and the University of Edinburgh, and was co-sponsored by the International Union of Geodesy and Geophysics, the International Association of Meteorology and Atmospheric Sciences, and the World Meteorological Organization.

关键词： NERC The Quadrennial Ozone Symposium 2016 UK USA

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Micro Flow-Through Thermocycler with Simple Meandering Channel with Symmetric Temperature Zones for Disposable PCR-Devices in Microscope Slide Format

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Journal of Bionic Engineering 2008年第4期5卷 291-298页

作者： Anett Reichert Jana Felbel Mark Kielpinski Matthias Urban Benno steinbrecht Thomas Henkel Microfluidics Department Institute of Photonic Technology Arvato Digital Services Manufacturing EMEA

Chip-based flow-through PCR implements the PCR as a continuous process for nucleic acid analytics. The sample is transported in a winding channel through temperature zones required for denaturation, annealing and extension. Main fields of application are the monitoring of continuous processes for rapid identification of contaminants and quality control as well as high throughput screening of cells or microorganisms. A modular arrangement with five heating zones for flow-through PCR is discussed and evaluated. The special heater arrangement allows the implementation of up to 40 cycles on the footprint of a microscope slide, which is placed on top ofa 5 zones heating plate. Liquid/liquid two phase flow of PCR reaction mixture and mineral oil have been applied to create a segmented flow process scheme. In that way, the developed system may provide flow-through PCR as a unit operation for the droplet based microfluidics platform. The single use of disposable devices is commonly preferred due to the sensitivity of the PCR process to contaminations. All-glass microfluidic chips and disposable chip devices, made from polycarbonate as a replication with identically geometry, have been fabricated and tested. For the first time, microchannel geometries with nearly circular profile developed by all-glass technology have been transferred to mass fabrication by injection compression molding. Both devices have been successfully applied for the detection of the tumor suppressor gene p53. Although product yield and selectivity of the amplification process do not depend on the chip material, a well defined, reliable segmented flow regime could only be realized in the all-glass chip.

关键词： lab-on-a-chip PCR microfluidics two-phase-flow injection molding point-of-care biomedical

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蝗虫气味分子结合蛋白与化学感受蛋白的免疫定位

蝗虫气味分子结合蛋白与化学感受蛋白的免疫定位

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中国昆虫学会第六届全国青年学术讨论会

作者：金鑫张善干 R.Alexander steinbrecht 张龙中国农业大学昆虫系中国农业大学昆虫系德国马普行为生理学研究所中国农业大学昆虫系

本研究利用扫描电镜观察了东亚飞蝗成虫触角的4种类型的化学感受器。以重组蛋白为抗原制备了东亚飞蝗和沙漠蝗气味分子结合蛋白以及化学感受蛋白的抗体,通过免疫组织化学方法,使用多种抗体研究了气味分子结合蛋白和化学感受蛋白在东亚... 详细信息

本研究利用扫描电镜观察了东亚飞蝗成虫触角的4种类型的化学感受器。以重组蛋白为抗原制备了东亚飞蝗和沙漠蝗气味分子结合蛋白以及化学感受蛋白的抗体,通过免疫组织化学方法,使用多种抗体研究了气味分子结合蛋白和化学感受蛋白在东亚飞蝗成虫及蝗蝻,以及沙漠蝗成虫不同类型化学感受器中定位。气味分子结合蛋白特异的表达在蝗蝻（3、4、5龄）和成虫的触角中。化学感受蛋白的表达则比较普遍,不同亚类的表达模式也有不同。气味分子结合蛋白位于毛形和锥形感受器的淋巴液中,化学感受蛋白-Ⅰ,Ⅱ和Ⅲ位于刺形感受器的外淋巴腔和上皮细胞与触角表皮间的亚表皮间隔中。虫体其他部位的刺形感受器也有化学感受蛋白-Ⅰ的表达。

关键词：气味分子结合蛋白化学感受蛋白免疫组织化学定位感受器蝗虫

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