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Reshaping of the Arabidopsis thaliana Proteome Landscape and Co-regulation of Proteins in Development and Immunity

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Molecular Plant 2020年第12期13卷 1709-1732页

作者： Mona Bassal Mohammad Abukhalaf Petra Majovsky Domenika Thieme Tobias herr Mohamed Ayash Naheed Tabassum MhD Rami Al Shweiki Carsten Proksch Ali hmedat Jorg Ziegler Justin Lee Steffen Neumann wolfgang hoehenwarter Leibniz Institute of Plant Biochemistry Biochemistry of Plant Interactions DepartmentProteome Biology of Plant Interactions Research GroupWeinberg 3Halle/Saale D-06120Germany Früh Verpackungstechnik AG FehrarltorfSwitzerland King Abdullah University of Science and Technology ThuwalSaudi Arabia Ulm University UlmGermany Universitatsklinikum halle(Saale) Sektion TumorbiologieHalle/SaaleGermany Molecular Signal Integration Department HalleGermany

Proteome remodeling is a fundamental adaptive response,and proteins in complexes and functionally related proteins are often *** a deep sampling strategy we define core proteomes of Arabidopsis thaliana tissues with around 10 000 proteins per tissue,and absolutely quantify(copy numbers per cell)nearly 16 000 proteins throughout the plant lifecycle.A proteome-wide survey of global post-trans-lational modification revealed amino acid exchanges pointing to potential conservation of translational infi-delity in *** analysis of protein abundance uncovered potentially new tissue-and age-specific roles of entire signaling modules regulating transcription in photosynthesis,seed development,and senescence and *** others,the data suggest a potential function of RD26 and other NAC transcription factors in seed development related to desiccation tolerance as well as a possible function of cysteine-rich receptor-like kinases(CRKs)as ROS sensors in *** of the components of ribo-some biogenesis factor(RBF)complexes were found to be co-expressed in a tissue-and age-specific manner,indicating functional promiscuity in the assembly of these less-studied protein complexes in ***,we characterized detailed proteome remodeling in basalimmunity bytreatingAra-bidopsis seeldings with *** simultaneously monitoring phytohormone and transcript changes upon flg22 treatment,we obtained strong evidence of suppression of jasmonate(JA)and JA-isoleucine(JA-Ile)levels by deconjugation and hydroxylation by IAA-ALA RESISTANT3(IAR3)and JASMONATE-INDUCED OXYGENASE 2(JOX2),respectively,under the control of JASMONATE INSENSITIVE 1(MYC2),sug-gesting an unrecognized role of a new JA regulatory switch in pattern-triggered *** together,the datasets generated in this study present extensive coverage of theArabidopsis proteome in various bio-logical scenarios,providing a rich resource available to the whole plant s

关键词： Arabidopsis deep proteomics protein co-expression plant immunity jasmonate

Induction of Apoptosis in Lung Cancer Cells by TRAIL and L-leucyl-L-leucine Methyl Ester

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Journal of Cancer Therapy 2011年第3期2卷 418-430页

作者： X. Sarah Sun Laura Bandura-Morgan wolfgang Zacharias 不详

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor superfamily that induces apoptosis in many tumor cells. Previous studies suggested that TRAIL treatment might also cause release of lysosomal cathepsin proteases to the cytosol, thus further promoting apoptosis. L-leucyl-L-leucine methyl ester (LeuLeuOMe) is a lysosome-destabilizing agent that may cause release of cathepsins into the cytosol and ensuing apoptosis. We hypothesized that a combination of TRAIL and LeuLeuOMe may synergistically promote apoptosis in lung cancer cells. The human epidermoid lung carcinoma cell line Calu-1 (TRAIL-resistant) and human large cell lung carcinoma cell line NCI-h460 (TRAIL-sensitive) were assayed for sensitivity to TRAIL and LeuLeuOMe, given alone or in different combination doses. Each agent alone induced a dose-dependent cytotoxicity, with substantially different efficacies of the two agents for the two cell types. When both agents were combined, synergistic cytotoxicity was achieved even in the TRAIL-resistant cells. TRAIL-induced cytotoxicity was completely inhibited by pan-caspase inhibitor z-VAD-fmk, but not by cysteine protease inhibitor E-64d. Conversely, E-64d totally blocked LeuLeuOMe-induced cytotoxicity. TRAIL caused mitochondrial damage, while enlarged lysosomes and lysosomal rupture were observed in LeuLeuOMe-treated cells. Our data suggest that, while TRAIL and LeuLeuOMe cause apoptosis through pathways that differ in their involvement of lysosomal cysteine proteases, mitochondrial and lysosomal desta-bilization have converging pro-apoptotic effects. Thus, the synergy of TRAIL and LeuLeuOMe may be used therapeutically to promote apoptosis in lung cancers, even those with intrinsic or acquired resistance to TRAIL.

关键词： Apoptosis Lung Cancer Lysosome Mitochondria TRAIL

Regulation of RNase E during the UV stress response in the cyanobacterium Synechocystis *** 6803

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mLife 2023年第1期2卷 43-57页

作者： Satoru Watanabe Damir Stazic Jens Georg Shota Ohtake Yutaka Sakamaki Megumi Numakura Munehiko Asayama Taku Chibazakura Annegret Wilde Claudia Steglich wolfgang R.hess Faculty of Biology Genetics and Experimental BioinformaticsUniversity of FreiburgFreiburgGermany Department of Bioscience Tokyo University of AgricultureSetagayakuTokyoJapan School of Agriculture Molecular GeneticsIbaraki UniversityIbarakiJapan Faculty of Biology Molecular GeneticsUniversity of FreiburgFreiburgGermany Nexxiot Prime Tower(Hardstrasse 201)ZurichSwitzerland.

Endoribonucleases govern the maturation and degradation of RNA and are indispensable in the posttranscriptional regulation of gene expression.A key endoribonuclease in Gram-negative bacteria is RNase *** ensure an appropriate supply of RNase E,some bacteria,such as Escherichia coli,feedback-regulate RNase E expression via the rne 5′-untranslated region(5′UTR)in ***,the mechanisms involved in the control of RNase E in other bacteria largely remain *** rely on solar light as an energy source for photosynthesis,despite the inherent ultraviolet(UV)*** this study,we first investigated globally the changes in gene expression in the cyanobacterium Synechocystis *** ^(6)803 after a brief exposure to *** the 407 responding genes 2 h after UV exposure was a prominent upregulation of rne mRNA ***,the enzymatic activity of RNase E rapidly increased as well,although the protein stability *** unique response was underpinned by the increased accumulation of full-length rne mRNA caused by the stabilization of its 5′UTR and suppression of premature transcriptional termination,but not by an increased transcription *** of RNA 3′ends and in vitro cleavage assays revealed that RNase E cleaves within a stretch of six consecutive uridine residues within the rne 5′UTR,indicating *** observations suggest that RNase E in cyanobacteria contributes to reshaping the transcriptome during the UV stress response and that its required activity level is secured at the RNA level despite the enhanced turnover of the protein.

关键词： cyanobacteria protein turnover ribonuclease stress response

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ZF帕骚——有效的连接

传动技术 2005年第4期19卷 16-20,26页

作者： wolfgang Rebholz ZF Passau Gmb h Fried richsha fen Germany ZF Passau Gmbh FriedrichshafenGermany

ZF帕骚公司最新的工程车辆用变速器Ergocontrol集成了传动系管理系统、运行数据连续记录仪和发动机管理系统等。使得该变速器性能实现了一个跳跃式的突破。

关键词：工程车辆传动系统系统集成

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创作有感染力的MV

DV@时代 2010年第4期 98-101页

作者： h. wolfgang Porter 薛冬梅（译）

当绝大多数的新晋电影制作人想到制作一支MV时，浮现在他脑海里的可能多是美丽的舞者，充满异国情调的地点和吸引人的歌手。

关键词： MV 创作风格艺术风格音乐

MORTl/FADD is involved in liver regeneration

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World Journal of Gastroenterology 2005年第46期11卷 7248-7253页

作者： Marcus Schuchmann Felix Rückert Jose F Garcia-Lazaro Andrea Karg Jürgen Burg Natalia Knorr Jürgen Siebler Eugene E Varfolomeev David Wallach wolfgang Schreiber Ansgar W Lohse Peter R Galle Department of Medicine University of Mainz Langenbeckstr.1Mainz 55101 Germany Institute of RadiologyUniversity of Mainz Langenbeckstr.1Mainz 55101Germany Institute of PathologyUniversity of Mainz Langenbeckstr. 1 Mainz 55101 Germany Department of MedicineUniversity of Mainz Langenbeckstr.1Mainz 55101Germany GenentechSouth San Francisco CAUnited States The Weizmann Institute of ScienceRehovot Israel Institute of RadiologyUniversity of Mainz Langenbeckstr.1Mainz 55101 Germany Department of Medicine University of Mainz Langenbeckstr.1Mainz 55101 Germany

AIM: To explore the role of the adaptor molecule in liver regeneration after partial hepatectomy (Ph). METhODS: We used transgenic mice expressing an N-terminal truncated form of MORT1/FADD under the control of the albumin promoter. As previously shown, this transgenic protein abrogated CD95- and CD120a-mediated apoptosis in the liver. Cyclin A expression was detected using Western blotting. ELISA and RT-PCR were used to detect IL-6 and IL-6 mRNA, respectively. DNA synthesis in liver tissue was measured by BrdU staining. RESULTS: Resection of 70% of the liver was followed by a reduced early regenerative response in the transgenic group at 36 h. Accordingly, 36 h after hepatectomy, cyclin A expression was only detectable in wild-type animals. Consequently, the onset of liver mass restoration was retarded as measured by MRI volumetry and mortality was significantly higher in the transgenic group. CONCLUSION: Our data demonstrate for the first time an involvement of the death receptor molecule MORT1/ FADD in liver regeneration, beyond its well described role as part of the intracellular death signaling pathway.

关键词： MORT1/FADD CD95 TNF Apoptosis Liver regeneration

Upper Ocean hydrology in the Indo-Pacific Warm Pool During the Late holocene,Early holocene and Last Glacial Maximum

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地学前缘 2009年第S1期16卷 224-224页

作者： wolfgang Kuhnt Ann holbourn Nils Andersen Institut für Geowissenschaften Christian-Albrechts-Universitt zu Kiel Institut für Geowissenschaften Christian-Albrechts-Universitat zu Kiel Leibniz-Labor für Altersbestimmung und Isotopenforschung

We examined sediment samples from twenty-four stations in the Indo-Pacific warm pool(IPWP)region. Our objective is to provide a better understanding of changes in the IPWP and related tropical climatic phenomena such as East Asian-Australian monsoon and El Ni(?)o-Southern Oscillation(ENSO),through improving spatial coverage of proxy *** order

关键词： paleoceanography upper ocean column Indo-Pacific warm pool late- and early-h-olocene last glacial maximum

α-SiO_x∶h钝化Cz-Si表面的工艺优化与机制分析

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功能材料 2014年第9期45卷 101-103页

作者：黄海宾张东华汪已琳龚洪勇高江 wolfgang R.Fahrner 周浪南昌大学太阳能光伏学院南昌330031

氢化非晶氧化硅(α-SiOx∶h)是一种优质的硅片表面钝化材料。采用PECVD法,以Sih4、CO2和h2作为气源制备α-SiOx∶h薄膜钝化Cz-Si表面,研究了沉积气压和CO2∶Sih4流量比对钝化效果的影响规律及作用机制。采用准稳态光电导法测试了硅片的... 详细信息

氢化非晶氧化硅(α-SiOx∶h)是一种优质的硅片表面钝化材料。采用PECVD法,以Sih4、CO2和h2作为气源制备α-SiOx∶h薄膜钝化Cz-Si表面,研究了沉积气压和CO2∶Sih4流量比对钝化效果的影响规律及作用机制。采用准稳态光电导法测试了硅片的有效少子寿命并依此计算出其表面复合速率以对薄膜的钝化效果进行定量表征,采用光谱型椭偏仪测试了样品的介电常数虚部ε2谱对样品微观结构进行了定性分析。结果表明,(1)在所研究范围内,氧掺入非晶硅薄膜使得薄膜结构趋向非晶化,沉积气压主要对薄膜中的空位浓度造成影响,而CO2/Sih4流量比的增加可增加薄膜中的h含量并改变了硅氢键的结构,从而影响薄膜的钝化效果;(2)在CO2/Sih4流量比为3.0/3.0mL/min,沉积气压为22Pa条件下获得了最优钝化效果,钝化后硅片有效少子寿命为975μs,表面复合速率为3.9cm/s。

关键词：氢化非晶氧化硅 PECVD 硅片表面钝化空位浓度氢含量

Microglial Dystrophy in the Aged and Alzheimer's Disease Brain Is Associated with Ferritin Immunoreactivity

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神经损伤与功能重建 2008年第4期3卷 253-264页

作者： KRYSLAINE O.LOPES D.LARRY SPARKS wolfgang J.STREIT Department of Neuroscience University of Florida College of MedicineGainesville Sun health Research Institute Sun City

小胶质细胞变性对认识衰老相关的神经退变和神经退行性疾病的发病机制非常重要。本研究通过铁蛋白免疫组织化学方法来分析非痴呆和阿茨海默病患者大脑中的小胶质细胞形态特征。作者的主要假设为,铁储存蛋白-铁蛋白的表达提高小胶质细胞... 详细信息

小胶质细胞变性对认识衰老相关的神经退变和神经退行性疾病的发病机制非常重要。本研究通过铁蛋白免疫组织化学方法来分析非痴呆和阿茨海默病患者大脑中的小胶质细胞形态特征。作者的主要假设为,铁储存蛋白-铁蛋白的表达提高小胶质细胞对退化的敏感性,尤其是在老年大脑中,因为衰老的小胶质细胞越来越无力维持铁环境稳定,而游离铁可促进氧化损伤。在24例34-97岁的病例中,小胶质细胞对铁蛋白的免疫反应被发现组成一个较大的hLA-DR抗体标记的小胶质细胞池。在老年尤其是AD大脑中,铁蛋白阳性的大部分小胶质细胞呈现出异常的形态学变化,即营养不良。铁蛋白阳性的营养不良小胶质细胞和AD组织中的老年斑之间并未发现空间相关性。对平均死亡时间(10.94±5.69)h的人脑组织的研究显示,小胶质细胞营养不良的出现不依赖于死亡时间,因而不是组织自溶的产物。这些结果均提示,包含铁储存和新陈代谢的小胶质细胞的变性可能是通过其高暴露于氧化应激。作者推论,铁蛋白免疫组织化学法可能是检测人脑小胶质细胞退行性变的有效方法。

关键词：小胶质细胞变性衰老铁老化