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Fast fluorescence lifetime imaging techniques:A review on challenge and development

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Journal of Innovative Optical Health Sciences 2019年第5期12卷 3-29页

作者： Xiongbo Liu Danying Lin wolfgang becker Jingjing Niu Bin Yu Liwei Liu Junle Qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060 P.R.China becker&Hickl GmbH Nunsdorfer Ring 7-9 Berlin 12277Germany

Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early ***,there is a large interest in obtaining FLIM data within an acquisition time as short as ***,there is currently a technology that advances towards faster and faster FLIM ***,the maximum speed of a recording technique is only part of the *** acquisition time of a FLIM image is a complex function of many *** include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological *** from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.

关键词： Fluorescence lifetime imaging microscopy(FLIM) acquisitin time imaging speed dead time photon fficiency time domain frequency domain scanning wide-field imaging time-correlated single photon counting(TCSPC) gated detection gated image intensifer modulated inage intensifier SPAD array detector

时间相关单光子计数记录快速荧光寿命图像(英文)

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深圳大学学报（理工版） 2008年第3期25卷 238-243页

作者： Bertram SU Vicky KATSOULIDOU wolfgang becker becker&Hickl公司 Nahmitzer Damm 30德国柏林D-12277

基于时间相关单光子计数(TCSPC)的荧光寿命成像(FLIM)的获取时间取决于成像的图像尺寸、样品寿命的精度及样品计数率.对于高密度荧光团样品,如染色组织或植物细胞,当前可用的样品计数率与TCSPC荧光寿命成像技术最高的计数率接近.该文描... 详细信息

基于时间相关单光子计数(TCSPC)的荧光寿命成像(FLIM)的获取时间取决于成像的图像尺寸、样品寿命的精度及样品计数率.对于高密度荧光团样品,如染色组织或植物细胞,当前可用的样品计数率与TCSPC荧光寿命成像技术最高的计数率接近.该文描述了在高计数率下TCSPC性能,并估计计数损失及堆积效应.结果表明,整个体系的寿命误差比预料的要小.因此, TCSPC FLIM可在获取时间低于1s的情况下记录寿命图像.为增加FLIM时间序列记录,利用存储交换技术,在采集下一个光子期间读取正在记录的数据,使用两个平行TCSPC模块,可以每秒两幅图像记录荧光寿命时间序列.该技术可应用于活体植物组织叶绿素的瞬态测量.

关键词：荧光寿命成像技术时间相关单光子计数叶绿素瞬态测量

EVA NEXUS-Phaco performance study

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International Journal of Ophthalmology(English edition) 2024年第8期17卷 1447-1452页

作者： Lukas Feldhaus Andreas Ohlmann Stefan Kassumeh Siegfried Priglinger wolfgang Mayer Department of Ophthalmology University HospitalLudwig-Maximilians-University MunichMunich 80336Germany

AIM:To investigate a novel phacoemulsification system“EVA NEXUS”(D.O.R.C.,Dutch Opthalmic Research Center)in comparison to the existing system“EVA”in clinical *** to compare both phacoemulsification systems in terms of efficiency,safety and postoperative inflammatory ***:In this study standardized cataract surgery was performed on both eyes of the study participant,using the“EVA system”(control group,n=20)on one eye and the“EVA NEXUS system”(intervention group,n=20)on the other *** patients with cataract LOCS Grading 1-3 and no accompanying eye diseases were included in this study.A total of 20 patients were included in this study,with each treatment arm including 20 *** surgery a 0.1 mL aqueous humor sample was collected 1min after phacoemulsification to measure the total prostaglanin E2 concentrations using an enzyme-linked immunosorbent *** endothelial cell count,visual and refractive outcomes,and anterior chamber flare were evaluated preoperatively,and 1d,1wk,and 3mo ***:There were no statistically significant differences between both groups regarding intraoperative safety parameters including effective phacoemulsification time(P=0.904),balanced saline solution flow(P=0.701)and total surgery time(P=0.565).Postoperative prostaglandin E2 levels,anterior chamber flare as well as endothelial cell loss tended to be lower in the NEXUS-Group,however not being statistically significant(P=0.718;0.164;0.486).Both systems provided similar clinical outcomes,regarding best corrected visual acuity and refractive parameters,showing no statistically significant differences between both ***:Both systems show a high level of safety and efficency with similar results in terms of safety parameters including postoperative inflammatory activity and endothelial cell loss as well as visual and refractive *** statistically not significant,the EVA NEXUS system tends to cause less postoperative inflammatio

关键词： cataract surgery phacoemulsification fluidics instrument

Going deep:Roots,carbon,and analyzing subsoil carbon dynamics

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Molecular Plant 2024年第1期17卷 1-3页

作者： Ezhilmathi Angela Joseph Fernando Michael Selvaraj Yusaku Uga wolfgang Busch Hunt Bowers Joe Tohme Alliance of Bioversity International and CIAT Km 17 Recta Cali-PalmiraAprtado Aereo 6713Cali 763537Colombia National Institute of Agrobiological Sciences(NIAS) TsukubaIbaraki 305-0856Japan Salk Institute for Biological Studies 10010 N Torrey Pines RoadLa JollaCA 92037USA Earth Optics Suite 840ArlingtonVA 22202USA

Agricultural practices currently contribute to the emission of approximately 10%–12%atmospheric greenhouse gasses,with tillage agriculture accelerating soil disruption and carbon dioxide ***,various options exist,such as converting new lands for agriculture,promoting healthier plant-based diets,adopting advanced management technologies,and minimizing food ***,these measures alone are insufficient to ensure environmental sustainability within planetary boundaries.

关键词： soil measures carbon

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小粉料秤称量系统

橡塑技术与装备 2001年第10期27卷 13-14页

作者： wolfgang becker 胡萍

概述了小粉料从进料、卸载、无尘处理到称量、包装、打标签等的全过程控制系统。

关键词：小粉料称量系统进料卸载无尘处理全过程控制系统

Towards engineering a portable platform for laparoscopic pre-training in virtual reality with haptic feedback

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虚拟现实与智能硬件（中英文） 2024年第2期6卷 83-99页

作者： Hans-Georg ENKLER wolfgang KUNERT Stefan PFEFFER Kai-Jonas BOCK Steffen AXT Jonas JOHANNINK Christoph REICH Furtwangen University 78120 FurtwangenGermany University Hospital and Faculty of Medicine Tuebingen 72076 TuebingenGermany

Background Laparoscopic surgery is a surgical technique in which special instruments are inserted through small incision holes inside the *** some time,efforts have been made to improve surgical pre training through practical exercises on abstracted and reduced *** The authors strive for a portable,easy to use and cost-effective Virtual Reality-based(VR)laparoscopic pre-training platform and therefore address the question of how such a system has to be designed to achieve the quality of today's gold standard using real tissue *** VR controllers are limited regarding haptic *** haptic feedback is necessary or at least beneficial for laparoscopic surgery training,the platform to be developed consists of a newly designed prototype laparoscopic VR controller with haptic feedback,a commercially available head-mounted display,a VR environment for simulating a laparoscopic surgery,and a training *** To take full advantage of benefits such as repeatability and cost-effectiveness of VR-based training,the system shall not require a tissue sample for haptic *** is currently calculated and visually displayed to the user in the VR *** the prototype controller,a first axis was provided with perceptible feedback for test *** of the prototype VR controllers can be combined to simulate a typical both-handed use case,e.g.,laparoscopic suturing.A Unity based VR prototype allows the execution of simple standard *** The first prototype enables full operation of a virtual laparoscopic instrument in *** addition,the simulation can compute simple interaction *** challenges lie in a realistic real-time tissue simulation and calculation of forces for the haptic *** weaknesses were identified in the first hardware prototype,which will be improved in subsequent *** degrees of freedom of the controller are to be provided with haptic *** make forces t

关键词： Laparoscopic surgery Training Virtual reality Controller Haptic feedback Kinesthetic skills

Customized molecular tools to strengthen metabolic engineering of cyanobacteria

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Green Carbon 2024年第2期2卷 149-163页

作者： Stephan Klähn Franz Opel wolfgang R.Hess Department of Solar Materials Biotechnology Helmholtz Centre for Environmental Research–UFZLeipzig 04318Germany Genetics and Experimental Bioinformatics Albert-Ludwig University FreiburgFreiburg im Breisgau 79104Germany

Cyanobacteria are promising oxygenic phototrophs for the production of various *** their(photo)biotechnological exploitation,molecular tools are required,such as,for the introduction and expression of heterologous genes,or the modulation of enzyme activities or entire *** and strategies for the development of photosynthetic biomanufacturing technologies based on cyanobacteria have been extensively reviewed,as well as certain specialized aspects of their genetic ***,options for metabolic engineering of specific cyanobacterial cells are still less developed than those for other bacteria of biotechnological *** addition to the standard genetic toolbox for“classical”metabolic engineering,we emphasize certain aspects,including recently developed vector systems for the extrachromosomal maintenance of genes and approaches based on clustered regularly interspaced short palindromic repeats(CRISPR)*** highlight the development of custom molecular tools for specific strains or products,discuss the emerging use of small regulatory proteins that appear promising for advanced metabolic engineering approaches to promote specific product formation,and provide an overview of suitable online ***,we discuss the current trends in this field and indicate their potential,such as using suitable product sensors that enable systematic screening,and optimization approaches.

关键词： Metabolic engineering Cyanobacteria Synthetic plasmids Regulatory proteins Sensors

264米的美国魔鬼塔为什么全球登顶者不足百人？

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环球人文地理 2024年第13期 90-97页

作者：李嘉麟 Dan Gary GRP Imagery kellyvandellen Kyle samantha Jacob wolfgang flickr

在美国怀俄明州，有一座不足300米的“矮山”。别看它不高，论起攀登难度，就连珠峰登顶者都对它望洋兴叹。在美国怀俄明州东北部，有一座由火山岩和沉积岩构成的圆柱形岩石塔——魔鬼塔。它矗立在一望无际的草原上，显得格外突兀。谁...

在美国怀俄明州，有一座不足300米的“矮山”。别看它不高，论起攀登难度，就连珠峰登顶者都对它望洋兴叹。在美国怀俄明州东北部，有一座由火山岩和沉积岩构成的圆柱形岩石塔——魔鬼塔。它矗立在一望无际的草原上，显得格外突兀。谁能相信，近百年来，能够登上264米塔顶的登山者不足百人。那么，魔鬼塔是怎么形成的呢？这个问题，让好事者争论了一百多年。

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A Maintenance Service Improvement Approach Based on Queue Networks: Application

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Open Journal of Applied Sciences 2024年第2期14卷 425-440页

作者： Aslain Brisco Ngnassi Djami wolfgang Nzié Boukar Ousman Joseph Nkongho Anyi Ulrich Ngnassi Nguelcheu Department of Fundamental Sciences and Techniques of Engineer Chemical Engineering and Mineral Industries School University of Ngaoundere Ngaoundere Cameroon Laboratory of Mechanics Materials and Photonics National School of Agro-Industrial Sciences University of Ngaoundere Ngaoundere Cameroon Department of Electrical Engineering Energy and Automation National School of Agro-Industrial Sciences University of Ngaoundere Ngaoundere Cameroon Laboratory E3M Faculty of Industrial Engineering University of Douala Douala Cameroon Department of Physics Faculty of Science University of Ngaoundere Ngaoundere Cameroon

The quest to increase the performance of production systems that have become complex leads to the transfer to the maintenance function of the responsibility of guaranteeing the availability of such systems. Also, we will never stop saying that maintenance must integrate into all of the company’s initiatives, to affirm its role, which is to ensure greater availability and sustainability of the means of production. The objective of this paper is to evaluate the reliability and availability of a system without knowing the distribution law of the operating times. Among the methods for evaluating dependability criteria (Fault Trees, Petri Nets, etc.), we are interested in queues that have the advantage of taking into account functional dependencies, thus allowing a quantified optimization of maintenance. Indeed, queues make it possible to model parallel or sequential processes, implementing operations taking place at the same time or one after the other, meeting the needs of modeling production systems. The main result of this paper is the study of the influence of availability on the reliability of a multi-state production system.

关键词： Markov Chains Queues Availability Reliability Maintenance