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Customized molecular tools to strengthen metabolic engineering of cyanobacteria

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Green Carbon 2024年第2期2卷 149-163页

作者： Stephan Klähn Franz Opel wolfgang r.hess Department of Solar Materials Biotechnology Helmholtz Centre for Environmental Research–UFZLeipzig 04318Germany Genetics and Experimental Bioinformatics Albert-Ludwig University FreiburgFreiburg im Breisgau 79104Germany

Cyanobacteria are promising oxygenic phototrophs for the production of various *** their(photo)biotechnological exploitation,molecular tools are required,such as,for the introduction and expression of heterologous genes,or the modulation of enzyme activities or entire *** and strategies for the development of photosynthetic biomanufacturing technologies based on cyanobacteria have been extensively reviewed,as well as certain specialized aspects of their genetic ***,options for metabolic engineering of specific cyanobacterial cells are still less developed than those for other bacteria of biotechnological *** addition to the standard genetic toolbox for“classical”metabolic engineering,we emphasize certain aspects,including recently developed vector systems for the extrachromosomal maintenance of genes and approaches based on clustered regularly interspaced short palindromic repeats(CrISPr)*** highlight the development of custom molecular tools for specific strains or products,discuss the emerging use of small regulatory proteins that appear promising for advanced metabolic engineering approaches to promote specific product formation,and provide an overview of suitable online ***,we discuss the current trends in this field and indicate their potential,such as using suitable product sensors that enable systematic screening,and optimization approaches.

关键词： Metabolic engineering Cyanobacteria Synthetic plasmids regulatory proteins Sensors

regulation of rNase E during the UV stress response in the cyanobacterium Synechocystis *** 6803

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mLife 2023年第1期2卷 43-57页

作者： Satoru Watanabe Damir Stazic Jens Georg Shota Ohtake Yutaka Sakamaki Megumi Numakura Munehiko Asayama Taku Chibazakura Annegret Wilde Claudia Steglich wolfgang r.hess Faculty of Biology Genetics and Experimental BioinformaticsUniversity of FreiburgFreiburgGermany Department of Bioscience Tokyo University of AgricultureSetagayakuTokyoJapan School of Agriculture Molecular GeneticsIbaraki UniversityIbarakiJapan Faculty of Biology Molecular GeneticsUniversity of FreiburgFreiburgGermany Nexxiot Prime Tower(Hardstrasse 201)ZurichSwitzerland.

Endoribonucleases govern the maturation and degradation of rNA and are indispensable in the posttranscriptional regulation of gene expression.A key endoribonuclease in Gram-negative bacteria is rNase *** ensure an appropriate supply of rNase E,some bacteria,such as Escherichia coli,feedback-regulate rNase E expression via the rne 5′-untranslated region(5′UTr)in ***,the mechanisms involved in the control of rNase E in other bacteria largely remain *** rely on solar light as an energy source for photosynthesis,despite the inherent ultraviolet(UV)*** this study,we first investigated globally the changes in gene expression in the cyanobacterium Synechocystis *** ^(6)803 after a brief exposure to *** the 407 responding genes 2 h after UV exposure was a prominent upregulation of rne mrNA ***,the enzymatic activity of rNase E rapidly increased as well,although the protein stability *** unique response was underpinned by the increased accumulation of full-length rne mrNA caused by the stabilization of its 5′UTr and suppression of premature transcriptional termination,but not by an increased transcription *** of rNA 3′ends and in vitro cleavage assays revealed that rNase E cleaves within a stretch of six consecutive uridine residues within the rne 5′UTr,indicating *** observations suggest that rNase E in cyanobacteria contributes to reshaping the transcriptome during the UV stress response and that its required activity level is secured at the rNA level despite the enhanced turnover of the protein.

关键词： cyanobacteria protein turnover ribonuclease stress response

Development comprehensive prognosis models for malignant gliomas

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Development comprehensive prognosis models for malignant gli...

Polarised radio emission associated with hess J1912+101

中国抗癌协会神经肿瘤专业委员会第八届学术会议

作者： Kenneth r.hess Departments of Biostatistics University of Texas M.D.AndersonHoustonTXUSA Cancer Center

＜正＞Malignant gliomas represent a heterogeneous spectrum of tumor types with diverse patient survival ***-surgical therapies showed very little *** of prognosis models based on histology or tumor origin（astrocytic ...

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research in Astronomy and Astrophysics 2019年第3期19卷 123-130页

作者： wolfgang reich Xiao-Hui Sun Max-Planck-Institut für radioastronomie Department of Astronomy Yunnan Universityand Key Laboratory of Astroparticle Physics of Yunnan Province

The shell-type TeV source hess J1912+101 was tentatively identified as an old supernova remnant, but is missing counterparts at radio and other frequencies. We analysed the Sino-German Urumqiλ6 cm survey and the Effelsberg λ11 cm and λ21 cm surveys to identify radio emission from hess J1912+101 to clarify the question of a supernova origin. We find a partial shell of excessive polarisation at λ6 cm at the periphery of hess J1912+101. At λ11 cm, its polarised emission is faint and suffers from depolarisation, while at λ21 cm, no related polarisation is seen. We could not separate the shell's total intensity signal from the confusing intense diffuse emission from the inner Galactic plane. However, a high percentage of polarisation in the shell's synchrotron emission is indicated. Our results support earlier suggestions that hess J1912+101 is an old supernova remnant. The synchrotron emission is highly polarised,which is typical for evolved supernova remnants with low surface brightness.

关键词： radio continuum ISM-ISM individual objects hess J1912+101-ISM magnetic fields

Microenvironmental engineering:An effective strategy for tailoring enzymatic activities

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Chinese Journal of Chemical Engineering 2020年第8期28卷 2028-2036页

作者： Yifei Zhang Henry hess Department of Biomedical Engineering Columbia University351L Engineering Terrace1210 Amsterdam AvenueNew YorkNY 10027USA

rationally,engineering a favorable physicochemical microenvironment for enzymes has recently emerged as an effective strategy to improve their catalytic *** this review,we discuss four microenvironmental effects according to the mechanism of action:localizing and excluding reactants and regulators,regulating microenvironmental pH,creating a water-like microenvironment,and increasing the local *** mechanisms are enzyme-independent and can in principle be used in combination to tailor enzyme behaviors,offering new approaches to enabling,enhancing,and regulating enzyme catalysis in diverse applications without the need for genetic engineering.

关键词： Biocatalysis Enzyme Microenvironment Activity enhancement

An efficient method for unfolding kinetic pressure driven VISAr data

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High Power Laser Science and Engineering 2015年第3期3卷 50-58页

作者： M.hess K.Peterson A.Harvey-Thompson Sandia National Laboratories

Velocity Interferometer System for Any reflector(VISAr) [Barker and Hollenbach, J. Appl. Phys. 43, 4669(1972)]is a well-known diagnostic that is employed on many shock physics and pulsed-power experiments. With the VISAr diagnostic, the velocity on the surface of any metal flyer can be found. For most experiments employing VISAr, either a kinetic pressure [Grady, Mech. Mater. 29, 181(1998)] or a magnetic pressure [Lemke et al., Intl J. Impact Eng. 38,480(2011)] drives the motion of the flyer. Moreover, reliable prediction of the time-dependent pressure is often a critical component to understanding the physics of these experiments. Although VISAr can provide a precise measurement of a flyer's surface velocity, the real challenge of this diagnostic implementation is using this velocity to unfold the timedependent pressure. The purpose of this paper is to elucidate a new method for quickly and reliably unfolding VISAr data.

关键词： laser driven blast wave pulsed-power VISAr

A New Woman and Her Warlord： Li Dequan, Feng Yuxiang, and the Politics of Intimacy in Twentieth- Century China

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Frontiers of History in China 2016年第3期11卷 431-457页

作者： Kate Merkel-hess Departments of History and Asian Studies The Pennsylvania State University University Park PA16802 USA

This article proposes a new way of viewing republican-era warlords. Through an examination of the life ofLi Dequan, the second wife of warlord Feng Yuxiang, it displaces Feng from his typical military and political context, scrutinizing instead the ways that Feng and Li interwove the private intimacies of love, marriage, and family life into their public and political lives. In the republic, Feng and Li, like many prominent figures of the time, shared elements of their private lives with journalists and, through them, a broader reading public, posing for photographs with their children on their way to school and inviting reporters to family events. Feng and Li utilized this newfound intimacy between public and political leaders to cultivate public sympathy and support. By the early PrC, Li--following Feng＇s sudden 1948 death--was named the first Minister of Health of the People＇s republic of China and her roles as wife and romantic object fell away. Instead, she focused on mothering the nation. By the late twentieth century, emphasis on the Li and Feng romance reappeared in writings about the couple, and while these narratives drew on the republican-era stories, it was made to seem that Li＇s feminism rather than Feng＇s modernity had facilitated their true love. Though the warlords have often been seen as destructive, exploring Feng＇s and Li＇s lives demonstrates that factional militarists and their families contributed to a new political culture grounded in a gendered national narrative that merewrite family and nation.

关键词： warlords gender feminism marriage revolution emotion nationalism media

Is it necessary to fuse to the pelvis when correcting scoliosis in cerebral palsy?

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World Journal of Orthopedics 2022年第4期13卷 365-372页

作者： Shane F Strom Matthew C hess Achraf H Jardaly Michael J Conklin Shawn r Gilbert Department of Orthopaedics University of Alabama at BirminghamBirminghamAl 35294United States Department of Orthopaedics The Hughston Clinic/Hughston FoundationColumbusGA 31908United States

BACKGrOUND Neuromuscular scoliosis is commonly associated with a large pelvic *** in children with cerebral palsy is most commonly managed with posterior spinal instrumentation and *** consensus is reached regarding the proximal starting point of fusion,controversy exists as to whether the distal level of spinal fusion should include the pelvis to correct the pelvic *** To assess the role of pelvic fusion in posterior spinal instrumentation and fusion,particularly it impact on pelvic obliquity correction,and to assess if the rate of complications differed as a function of pelvic *** This was a retrospective,cohort study in which we reviewed the medical records of children with cerebral palsy scoliosis treated with posterior instrumentation and fusion at a single *** follow-up was six *** were stratified into two groups:Those who were fused to the pelvis and those fused to L4/*** major outcomes were complications and radiographic *** former were stratified into major and minor complications,and the latter consisted of preoperative and final Cobb angles,L5-S1 tilt and pelvic *** The study included 47 *** correction of the L5 tilt was 60%in patients fused to the pelvis and 67%in patients fused to L4/L5(P=0.22).The pelvic obliquity was corrected by 43%and 36%in each group,respectively(P=0.12).regarding complications,patients fused to the pelvis had more total complications as compared to the other group(63.0%vs 30%,respectively,P=0.025).After adjusting for differences in radiographic parameters(lumbar curve,L5 tilt,and pelvic obliquity),these patients had a 79%increased chance of developing complications(relative risk=1.79;95%CI:1.011-3.41).CONCLUSION Including the pelvis in the distal level of fusion for cerebral palsy scoliosis places patients at an increased risk of postoperative *** added value that pelvic fusion offers in terms of correcting

关键词： Cerebral palsy Scoliosis Pelvic fusion Pelvic obliquity Spinal fusion Distal lumbar level

Lucio Fontana 1899-1968

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Lucio Fontana 1899-1968