检索结果-南通市图书馆

Discovery of the inhibitor of DNA binding 1 as a novel marker for radioresistance in pancreatic cancer using genome-wide rNA-seq

引用

Cancer Drug resistance 2022年第4期5卷 926-938页

作者： scar Zuniga Stephanie Byrum Adam r.wolfe Department of radiation Oncology University of Arkansas for Medical SciencesLittle RockAR 72205USA Department of Biochemistry and Molecular Biology University of Arkansas for Medical SciencesLittle RockAR 72205USA

Purpose/Objective(s): Discovery of genetic drivers of radioresistance is critical for developing novel therapeuticstrategies to combine with radiotherapy of radioresistant PDAC. In this study, we used genome-wide rNA-seq toidentify genes upregulated in generated radioresistant PDAC cell lines and discovered the Inhibitor of DNA Binding1 (ID1) gene as a potential regulator of radioresistance in ***/Methods: radioresistant clones of the PDAC cell lines MIA PaCa-2 and PANC-1 were generated bydelivering daily ionizing irradiation (Ir) (2 Gy/day) in vitro over two weeks (total 20 Gy) followed by standardclonogenic assays following one week from the end of Ir. The generated rr and parental cell lines were submittedfor rNA-seq analysis to identify differentially expressed genes. The Limma r package was used to calculatedifferential expression among genes. Log2 fold change values were calculated for each sample compared to thecontrol. Genes with an absolute fold change > 1 were considered significant. rNA sequencing expression data fromthe Cancer Genome Atlas (TCGA) database was analyzed through the online databases GEPIA, cBioPortal, and theHuman Protein ***: Following exposure to two weeks of 2 Gy daily Ir in vitro, the two PDAC cell lines showed significantlygreater clonogenic cell survival than their parental cell lines, indicating enhanced rr in these cells. rNA-seqanalysis comparing parental and rr cell lines found upregulated seven genes (TNS4, ZDHHC8P1, APLNr, AQP3,SPP1, ID1, ID2) and seven genes downregulated (PTX3, ITGB2, EPS8L1, ALDH1L2, KCNT2, ArHGAP9, IFI16) in bothrr cell lines. Western blotting confirmed increased expression of the ID1 protein in the rr cell lines compared totheir parental cell lines. We found that ID1 mrNA was significantly higher in PDAC tumors compared to matchednormal and high ID1 expression correlated with significantly worse disease-free survival (DFS) in PDAC patients(Hr = 2.2, log rank P = 0.009). ID1 mrNA express

关键词： Pancreatic cancer radiation resistance ID1 rNA-seq

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

不对称光纤非互易相移的全光纤磁-光隔离器

引用

光纤与电缆及其应用技术 1996年第2期 42-44,53页

作者： r.wolfe 鲍赛红美

据我们所知,本文第一个提出并观察到光纤中存在新的磁-光效应.以前人们在平面波导的TM模中发现过垂直磁场内存在非互易相移,最近我们在可传输类TM模的不对称纤芯的光纤(如纤芯一侧掺钬的特殊光纤)中也发现这种现象.提出了单偏振和偏振... 详细信息

据我们所知,本文第一个提出并观察到光纤中存在新的磁-光效应.以前人们在平面波导的TM模中发现过垂直磁场内存在非互易相移,最近我们在可传输类TM模的不对称纤芯的光纤(如纤芯一侧掺钬的特殊光纤)中也发现这种现象.提出了单偏振和偏振无关的新颖的全光纤隔离器,这种隔离器是基于在马赫-曾德干涉仪中采用这些特殊光纤.该效应还可用于环行器和萨格奈克效应光纤陀螺仪.

关键词：隔离器磁光效应光纤元件

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Emerging structures and dynamic mechanisms ofγ-secretase for Alzheimer’s disease

引用

Neural regeneration research 2025年第1期20卷 174-180页

作者： Yinglong Miao Michael S.wolfe Computational Medicine Program and Department of Pharmacology University of North Carolina–Chapel HillChapel HillNCUSA Department of Medicinal Chemistry University of KansasLawrenceKSUSA

γ-Secretase,called“the proteasome of the membrane,”is a membrane-embedded protease complex that cleaves 150+peptide substrates with central roles in biology and medicine,including amyloid precursor protein and the Notch family of cell-surface *** inγ-secretase and amyloid precursor protein lead to early-onset familial Alzheimer’s disease.γ-Secretase has thus served as a critical drug target for treating familial Alzheimer’s disease and the more common late-onset Alzheimer’s disease as ***,critical gaps remain in understanding the mechanisms of processive proteolysis of substrates,the effects of familial Alzheimer’s disease mutations,and allosteric modulation of substrate cleavage byγ-*** this review,we focus on recent studies of structural dynamic mechanisms ofγ-*** mechanisms,including the“Fit-Stay-Trim,”“Sliding-Unwinding,”and“Tilting-Unwinding,”have been proposed for substrate proteolysis of amyloid precursor protein byγ-secretase based on all-atom molecular dynamics *** an incorrect registry of the Notch1 substrate was identified in the cryo-electron microscopy structure of Notch1-boundγ-secretase,molecular dynamics simulations on a resolved model of Notch1-boundγ-secretase that was reconstructed using the amyloid precursor protein-boundγ-secretase as a template successfully capturedγ-secretase activation for proper cleavages of both wildtype and mutant Notch,being consistent with biochemical experimental *** approach could be potentially applied to decipher the processing mechanisms of various substrates byγ-*** addition,controversy over the effects of familial Alzheimer’s disease mutations,particularly the issue of whether they stabilize or destabilizeγ-secretase-substrate complexes,is ***,an outlook is provided for future studies ofγ-secretase,including pathways of substrate binding and product release,effects of modulators on familial Alzheimer’s disease mutations

关键词： Alzheimer’s disease amyloid precursor protein cryo-EM structures drug design intramembrane proteolysis molecular dynamics Notch

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

阿司匹林对健康老年人心血管疾病和出血的影响

引用

中华预防医学杂志 2019年第11期53卷 1187-1187页

作者：谢双华(编译) McNeil JJ wolfe r Woods rL 国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院肿瘤登记办公室不详

阿司匹林是公认的心血管疾病二级预防疗法之一,但它的一级预防作用,特别是在心血管风险较高的老年人群中的一级预防作用尚不清楚。本研究为一项随机、双盲、安慰剂对照试验,2010—2014年从澳大利亚和美国招募70岁以上(美国黑人和西班牙... 详细信息

阿司匹林是公认的心血管疾病二级预防疗法之一,但它的一级预防作用,特别是在心血管风险较高的老年人群中的一级预防作用尚不清楚。本研究为一项随机、双盲、安慰剂对照试验,2010—2014年从澳大利亚和美国招募70岁以上(美国黑人和西班牙裔年龄≥65岁)、没有心血管疾病、痴呆或残疾的社区居民,并将参与者随机分配至100 mg肠溶阿司匹林组和安慰剂组;主要观察终点为死亡、痴呆或持续性身体残疾,次要观察终点包括大出血和心血管疾病(包括致命性冠心病、非致命性心肌梗死、致命性或非致命性中风或心力衰竭住院)。

关键词：安慰剂对照观察终点肠溶阿司匹林身体残疾心血管疾病心血管风险随机分配非致命性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

居住在高海拔地区有潜在心肺功能障碍性疾病及肺动脉高压儿童的高原肺水肿情况

引用

世界核心医学期刊文摘（儿科学分册） 2005年第5期1卷 33-34页

作者： Das B. B. wolfe r. r. Chan K. -C. D. Ivy 虎小毅 Department of Pediatr ic Cardiology Children's Hospital 1056 E 19th Ave Denver CO 80218 United S tatesDr.

Background: Pulmonary hypertension has not been described as a predisposing ri sk factor for high-altitude pulmonary edema (HAPE) in children. Previous studie s have shown an association of HAPE with abnormally increased pulmonary vasoreac tivity to hypoxia but generally normal pulmonary artery pressure (PAP) after rec overy. Objective: To describe HAPE of relatively rapid onset and its management in a series of children residing at moderate to high altitudes, all of whom had underlying pulmonary hypertension. Methods and results: From 1997 to 2003, 30 ch ildren came to our center with high-altitude illness. Of these, 10 children (ag ed 4-18 years; male-female ratio, 8:2) living at moderate to high altitudes (1 610-3050 m) -underwent cardiac catheterization after recovery from HAPE, and all were fou nd to have chronic pulmonary hypertension (mean PAP, 38 ±9 mm Hg; pulmonary vas cular resistance, 8.6 ±2.8 U ×m2). Increases in PAP and pulmonary vascular r esistance to hypoxia (16%oxygen) suggest that these children have a reactive pu lmonary pressor response and hence are susceptible to HAPE. Six of the 10 patien ts had predisposing cardiopulmonary abnormalities, and 5 of these 6 patients did not receive a diagnosis prior to the onset of HAPE. Long-term treatment with c alcium channel blockers, bosentan, sildenafil citrate, and/or oxygen lowered PAP , improved symptoms, and prevented the recurrence ofHAPE. Conclusion: Children l iving at altitude who develop HAPE should undergo screening for diagnosis of und erlying cardiopulmonary abnormalities including pulmonary hypertension.

关键词：高原肺水肿心肺功能障碍肺动脉高压肺血管阻力血管反应性心导管检查肺动脉压枸橼酸西地那非钙通道阻滞剂缺氧状态

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metabolic syndrome and the urinary microbiome of patients undergoing percutaneous nephrolithotomy

引用

Asian Journal of Urology 2024年第2期11卷 316-323页

作者： ryan A.Dornbier Chirag P.Doshi Shalin C.Desai Petar Bajic Michelle Van Kuiken Mark Khemmani Ahmer V.Farooq Larissa Bresler Thomas M.T.Turk Alan J.wolfe Kristin G.Baldea Loyola University Medical Center Department of UrologyMaywoodILUSA Loyola University Chicago Department of Microbiology and ImmunologyMaywoodILUSA

Objective:To identify possible stone-promoting microbes,we compared the profiles of microbes grown from stones of patients with and without metabolic syndrome(MetS).The association between MetS and urinary stone disease is well established,but the exact pathophysiologic relationship remains *** evidence suggests urinary tract dysbiosis may lead to increased nephrolithiasis ***:At the time of percutaneous nephrolithotomy,bladder urine and stone fragments were collected from patients with and without *** sample types were subjected to expanded quantitative urine culture(EQUC)and 16 S ribosomal rNA gene ***:Fifty-seven patients included 12 controls(21.1%)and 45 MetS patients(78.9%).Both cohorts were similar with respect to demographics and non-MetS *** controls had uric acid stone *** EQUC,bacteria were detected more frequently in MetS stones(42.2%)compared to controls(8.3%)(p=0.041).Bacteria also were more abundant in stones of MetS patients compared to *** validate our EQUC results,we performed 16 S ribosomal rNA gene *** 12/16(75.0%)sequence-positive stones,EQUC reliably isolated at least one species of the sequenced *** were detected in both“infectious”and“non-infectious”stone ***:Bacteria are more common and more abundant in MetS stones than control *** findings support a role for bacteria in urinary stone disease for patients with MetS regardless of stone composition.

关键词： Nephrolithiasis Urolithiasis Metabolic syndrome Urinary microbiome Percutaneous nephrolithotomy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

resilience, the 6th Vital Sign: Conceptualizing, Contextualizing, and Operationalizing All Six Vital Signs

引用

Health 2024年第7期16卷 657-673页

作者： rose E. Constantino Betty J. Braxter Chi Ching Vivian Hui Larissa C. Allen Lillian J. wolfe Katherine H. Endres Jezyl Cempron Cutamora Laurence L. Garcia Daisy r. Palompon Kathleen Thimsen Brayden N. Kameg Margarete L. Zalon Department of Health and Community Systems School of Nursing University of Pittsburgh Pittsburgh PA USA Undergraduate Affairs School of Nursing University of Pittsburgh Pittsburgh PA USA The Hong Kong Polytechnic University Hong Kong China School of Nursing Adjunct Faculty at the University of Pittsburgh Pittsburgh PA USA School of Nursing University of Pittsburgh Pittsburgh PA USA Department of Health Promotion and Development School of Nursing University of Pittsburgh Pittsburgh PA USA College of Nursing Cebu Normal University Cebu City Philippines School of Nursing University of Nevada Las Vegas USA Department of Health and Community Systems University of Pittsburgh PA USA Department of Nursing University of Scranton Scranton PA USA

There are five vital signs that healthcare providers assess: temperature, pulse, respiration, blood pressure, and pain. Normal levels for the five vital signs are published by the American Heart Association, and other specialty organizations, however, the sixth vital sign (resilience) which adopts the measure of immune resilience is suggested in this paper. resilience is the ability of the immune system to respond to attacks and defend effectively against infections and inflammatory stressors, and psychological resilience is the capacity to resist, adapt, recover, thrive, and grow from a challenge or a stressor. Individuals with better optimal immune resilience had better health outcomes than those with minimal immune resilience. The purpose of this paper is to conceptualize, contextualize, and operationalize all six vital signs. We suggest measuring resilience subjectively and objectively. Subjectively, use a 5-item guided interview revised from the Connor-Davidson resilience Scale (CDrC), a scale of 10 items. The revised CDrC scale is a 5-item scale. The scale is rated on a 5-point Likert scale from 0 (not true) to 4 (true all the time). The total score ranges from 0 to 20, with higher total scores indicating greater resilience. The scale demonstrated good construct validity and internal consistency (α = 0.85) during the development of the scale. The CD-rISC had a good Cronbach’s alpha level of 0.85. The revised CD-rISC can be completed in 2 - 4 minutes. To measure resilience objectively, we suggest using Immune resilience (Ir) levels, the level of resilience to preserve and/or rapidly restore immune resilience functions that promote disease resistance and control inflammation and other inflammatory stress. Ir levels are gauged with two peripheral blood metrics that quantify the balance between CD8 and CD4 T-cell levels and gene expression signatures tracking longevity-associated immunocompetence and mortality- or entropy-associated inflammation. Ir deregulation i

关键词： Conceptualization Contextualization Operationalization Body Temperature Pulse respiration Blood Pressure Pain resilience

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

无心血管病老年人群的高血压治疗和控制相关影响因素:一项横断面研究

引用

中华高血压杂志(中英文) 2021年第4期29卷 398-398页

作者：周卫叶鹏 Chowdhury EK Nelson Mr Ernst ME Margolis KL Beilin LJ Johnston CI Woods rL Murray AM wolfe r Storey E Shah rC Lockery JE Tonkin AM Newman AB Williamson JD Abhayaratna WP Stocks NP Fitzgerald SM Orchard SG Trevaks rE Donnan GA Grimm r McNeil JJ reid CM

虽已有各种降压疗法,但在老龄化人口中,血压控制仍不够理想。另外,人们对老年人群高血压管理的认识也有差距。研究者探讨无明显心血管病的老年高血压患者的降压治疗和控制情况,试图确定"未治疗"和"治疗但未控制"高血压的相关因素。方法...

虽已有各种降压疗法,但在老龄化人口中,血压控制仍不够理想。另外,人们对老年人群高血压管理的认识也有差距。研究者探讨无明显心血管病的老年高血压患者的降压治疗和控制情况,试图确定"未治疗"和"治疗但未控制"高血压的相关因素。方法:研究者分析了参加老年人阿司匹林减少心血管事件研究的年龄≥65岁,来自澳大利亚和美国的参与者19 114例的基线数据。

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

reforming the World Trade Organization: Practitioner Perspectives from China, the EU, and the US

引用

China & World Economy 2021年第4期29卷 1-34页

作者： Bernard Hoekman robert wolfe European University Institute and the Centre for Economic Policy research Italy Queen's University Canada

China,the European Union,and the United States are the world s largest *** have a big stake in a multilateral system of rules to manage the inevitable frictions among interdependent economies organized on different *** paper discusses elements of the WTO reform agenda through the lens of positions taken by these three WTO members,identifying the extent of alignment on key subjects,including transparency,dispute settlement,and plurilateral *** draw on findings of a recent research project on WTO reform and use responses to an expert survey to assess the prospects for actions that all three trade powers might *** premise is that reforming WTO is a necessary condition for the organization to be a more salient forum for the three large economies to address trade tensions,and that agreement among these three trade powers^in turn,is necessary to resolve the problems of the WTO.

关键词： China plurilateral cooperation trade agreements trade conflict WTO

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of aqueous extracts of Irvingia gabonensis seeds on the hormonal parameters of male guinea pigs

引用

Asian Pacific Journal of Tropical Medicine 2010年第3期3卷 200-204页

作者： Obianime Acubowyedia wolfe Uche Fidelia Ijeoma Department of Pharmacology University of Port Harcourt Nigeria Department of Pharmacognosy and Phytotherapy University of Port Harcourt Nigeria

Objective:To investigate the effects of the aqueous extracts of Irvingia gabonensis(Irvingaceae) seeds on the hormonal parameters of male guinea ***:The hormonal effects of Irvingia gabonensis on hormonal parameters of male guinea pigs were investigated and compared with that of proviron using enzyme immuno assay method,which was done by reaction of antibody with serum testosterone and testosterone label,magnetic solid phase separation and colour development *** phytochemical screening of Irvingia gabonensis seeds was also carried out using standard ***:The aqueous extract of the Irvingia gabonensis seeds(50-400 mg/kg) caused a statistically significance increase(Prone in male guinea pigs,from (2.70±0.26) ng/mL to(3.10±0.42) ng/mL on the 7th day and to(3.30±0.48) ng/mL on the 28th day of the administration of the *** highest increase was(3.30±0.48) ng/mL,being obtained after 28 days of *** effects were similar to that of proviron,which was (2.80±0.28) ng/mL and(3.00±0.41) ng/mL on the 7th and 28th day of treatment respectively. The phytochemical screening of Irvingia gabonensis seeds revealed the presence of flavonoids. tannin,alkaloids,carbohydrate,volatile oils,terpenoids,saponins and cardiac glycosides. Conclusions:This study supports the claims on the use of the seeds of Irvingia gabonensis by traditional medicine practitioners as a fertility ***,further studies need to be done to isolate and characterize the active principle(s) responsible for this activity in this plant.

关键词： Irvingia gabonensis Hormonal parameters Male guinea pigs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：