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Science China(Life Sciences) 2019年第4期62卷 489-497页

作者： marten Kellner wojciech maka?owski Institute of Bioinformatics Faculty of Medicine University of Münster

Transposable elements(TEs) are major components of the human genome constituting at least half of it. more than half a century ago, Barbara mcClintock and later Roy Britten and Eric Davidson have postulated that they might be major players in the host gene regulation. We have scanned a large amount of data produced by ENCODE project for active transcription binding sites(TFBSs) located in TE-originated parts of polymerase II promoters. In total, more than 35,000 promoters in six different tissues were analyzed and over 26,000 of them harbored TEs. moreover, these TEs usually provide one or more of TFBSs in the host promoters, which resulted in more than 6% of active TFBSs in these regions located in the TE-originated sequences. Rewiring of transcription circuits played a major role in mammalian evolution and consequently increased their functional and morphological diversity. In this large-scale analysis, we have demonstrated that TEs contributed a large fraction of human TFBSs. Interestingly,these TFBSs usually act in a tissue-specific manner. Thus, our study clearly showed that TEs played a significant role in shaping expression pattern in mammals and humans in particular. Furthermore, since several TE families are still active in our genome,they continue to influence not only our genome architecture but also gene functioning in a broader sense.

关键词： transposable elements genome evolution gene expression evolution transcription factors transcription binding sites,ENCODE project promoters

The methodology of determining optimum access routes to remote areas for the purposes of crisis management

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International Journal of Digital Earth 2022年第1期15卷 1905-1928页

作者： wojciech Dawid Krzysztof Pokonieczny marek Wyszyński Faculty of Civil Engineering and Geodesy Institute of Geospatial Engineering and GeodesyMilitary University of TechnologyWarsawPoland

The article presents a methodology to determine access routes to places with difficult access,situated outside the regular transport *** determine the optimum route,the existing road network was used,along with terrain passability maps,as well as high-resolution digital terrain models together with the traction parameters of the vehicle to enable conducting a detailed analysis of micro-relief and excluding inaccessible *** research was extended to include a comprehensive analysis of terrain accessibility for areas located in the vicinity of the capital cities of three European countries on various levels of economic development:Germany,Poland,and *** degree of development of the analysed area was determined with the use of the gross domestic product(GDP),human development index(HDI),and the road quality *** case study presented in the paper illustrates the practical application of the developed methodology that was implemented to a real crisis *** routes were determined for a mAN TGm 15.290 vehicle that went to take part in emergency action in buildings situated approximately 500 m away from paved *** developed methodology may be widely used in crisis management,where the emergency actions often require rescue units to access remote places.

关键词： Crisis management routing terrain accessibility fire departments OSm

Deadwood affects the soil organic matter fractions and enzyme activity of soils in altitude gradient of temperate forests

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Forest Ecosystems 2023年第3期10卷 316-327页

作者： Ewa Błońska wojciech Prazuch Jarosław Lasota Department of Ecology and Silviculture Faculty of ForestryUniversity of Agriculture in Krakow29 Listopada 46 Str.31-425KrakowPoland

The main objective of our study has been to determine the role of deadwood in the shaping of the amount of soil organic matter fractions in mountain forest *** this purpose,a climosequence approach comprising north(N)and south(S)exposure along the altitudinal gradient(600,800,1000 and 1200 m a.s.l.)was set *** comparing the properties of decomposing deadwood and those of the soils located directly beneath the decaying wood we drew conclusions about the role of deadwood in the shaping of soil organic matter fractions and soil carbon storage in different climate *** basic properties,enzymatic activity and fractions of soil organic matter(SOm)were determined in deadwood and affected directly by the components released from decaying *** decomposed deadwood impacts soil organic matter stabilization more strongly than the less decayed deadwood and the light fraction of SOm is more sensitive to deadwood effects than the heavy fraction regardless of the location in the altitude *** in SOm mineral-associated fraction C content is more pronounced in soils under the influence of deadwood located in lower locations of warmer *** released from decaying wood stimulate the enzymatic activity of soils that are within the range of deadwood influence.

关键词： Enzyme activity Forest soils Heavy fraction Light fraction Soil organic matter

Concept of a swirling diffuser in batch blending tanks

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Chinese Journal of Chemical Engineering 2020年第2期28卷 378-382页

作者： wojciech Artichowicz Jerzy m.Sawicki Gdańsk University of Technology Faculty of Civil and Environmental EngineeringNarutowicza 11/1280-233 GdańskPoland

The mixing of two or more components belongs to the category of the most common unit operations, both in technology and in nature. One particular version is homogenization(blending), the effectivity of which is strongly related to the blending time. Among many differentiated individual solutions of mixing systems used in blenders, one should distinguish the class of agitators with diffusers. An analysis of the character of the velocity field during a blending chamber operation leads to the conclusion that it would be of avail to outfit a straight diffuser with different setups of elbows. In consequence, the vertical direction of the inflowing fluid stream would be changed into the horizontal direction of the outflowing stream, which should intensify the blending process. The concept has been verified experimentally, making use of the tracer methodology. The obtained results confirmed the theoretical conclusion that the blending time for the swirling-diffuser was shorter than for the classical straight one.

关键词： Batch blending chamber Swirling diffuser Blending time

Fe-N-C catalysts for oxygen electroreduction under external magnetic fields:Reduction of magnetic O_(2) to nonmagnetic H_(2)O

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Journal of Energy Chemistry 2022年第1期31卷 296-308,I0008页

作者： wojciech Kicinski Jakub P.Sek Agata Kowalczyk Sylwia Turczyniak-Surdacka Anna m.Nowicka Slawomir Dyjak Boguslaw Budner mikolaj Donten Institute of Chemistry Military University of Technology2 Kaliskiego Str.PL 00-908 WarsawPoland Faculty of Chemistry University of WarsawPasteura 1 StrPL 02-093 WarsawPoland Faculty of Chemistry Biological and Chemical Research CentreUniversity of WarsawZwirki i Wigury 101 StrPL 02-089 WarsawPoland Institute of Optoelectronics Military University of Technology.2 Kaliskiego StrPL 00-908 WarsawPoland

An extensive analysis of iron-nitrogen-carbon(Fe-N-C)electrocatalysts synthesis and activity is presented concerning synthesis conditions such as initial Fe content,pyrolysis temperature and atmosphere(inert N_(2),reducing NH_(3),oxidizing Cl_(2) and their sequential combinations)and the influence of an external magnetic field on their performance in oxygen reduction reaction(ORR).Thermosetting porous polymers doped with FeCl_(3) were utilized as the Fe-N-C catalysts *** pyrolysis temperature was varied within a 700-900℃*** temperature and atmosphere of pyrolysis strongly affect the porosity and compositi on of the resultant Fe-N-C catalysts,while the in itial amount of Fe precursor shows much weaker *** under NH_(3) yields materials similar to those pyrolyzed under an inert atmosphere(N_(2)).In contrast,pyrolysis under Cl_(2) yields carbon of peculiar character with highly disordered structure and extensive *** application of a static external magnetic field strongly enhances the ORR process(herein studied in an alkaline environment)and the enhancement correlates with the Fe content in the Fe-N-C *** Fe-N-C materials containing ferromagnetic iron phase embedded in N-doped microporous carbon constitute attractive catalysts for magnetic field-aided anion exchange membrane fuel cell technology.

关键词： Oxygen reduction reaction Fe-N-C catalyst PGm-free catalyst magnetic field Chronoamperometry Anion exchange membrane fuel cell (AEmFC)

microwave absorption properties of carbonyl iron-based paint coatings for military applications

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Defence Technology（防务技术） 2023年第4期22卷 1-9页

作者： wojciech Przybyl Adam Januszko Norbert Radek marcin Szczepaniak Krzysztof A.Bogdanowicz Ireneusz Plebankiewicz Bogusława Szczodrowska Robert mazurczuk military Institute of Engineer Technology ul.Obornicka 136Wrocław50-961Poland Kielce University of Technology Faculty of Mechatronics and Mechanical EngineeringAl.1000-lecia P.P.7Kielce25-314Poland

The paper explores the possibilities of using carbonyl iron in the form of a powder for the manufacture of radar-absorbing paints-reducing the radar signature of the objects that they *** attenuation values in the range of 4-18 GHz for various coating thicknesses,ranging from 0.5 to 2.00 mm with 0.5 mm increment,and for different absorber content-75%and 80%,as well as the use of two different binders in the form of epoxy resins with hardeners,were *** the frequency of 18 GHz and a 1.5 mm thick coating with a 75%absorber content,Epidian 112 resin and Saduramid 10/50 hardener used as a binder,and the maximum attenuation level obtained equalled 20.2 d B at 16 ***,the absorber particle size ranging from 3 to 4μm and its higher mass content resulted in achieving the reflection loss above-12 d B in the entire 8-12.5 GHz range for layers between 1-and 1.5 mm *** qualitative assessment of the tested samples in the context of camouflage in the radar range was also performed,using statistical analysis.

关键词： Radar Attenuation Carbonyl iron Coating Camouflage

Polarization effect in tip-enhanced infrared nanospect- roscopy studies of the selective Y5 receptor antagonist Lu AA33810

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Nano Research 2018年第8期11卷 4401-4411页

作者： Natalia Piergies Ewa Pinta Czestawa Paluszkiewicz Helena Domin wojciech m.Kwiatek Institute of Nuclear Physics Polish Academy of Sciences PL-31342 Krakow Poland Institute of Pharmacology Polish Academy of Sciences Department of Neurobiology 31-343 Krakow Smetna Street 12 Poland Natalia Piergies and Ewa Pieta contributed equally to this work.

A novel approach of combining conventional infrared spectroscopy （IR） and atomic force microscopy （AFm） is presented to better understand the behavior of a drug adsorbed on a metal substrate at the nanoscale level. Tip-enhanced infrared nanospectroscopy （TEIRA） was used for the first time to investigate Lu AA33810, a selective brain-penetrating Y5 receptor antagonist, after immobilization on gold nanopartides （GNPs）. Here, a gold coated AFm tip and gold substrate were used to obtain the near-field electromagnetic field trapping effect. Because of the huge signal enhancement, it was possible to obtain the spectral information regarding the self-assembled monolayer of the investigated molecule. The effect of two orthogonal polarizations （p- and s-polarization modulations） of the excitation laser beam on the spectral patterns is also discussed. The results show that there is a strong relationship between the state of polarization of the incident radiation and the relative infrared band intensities. Another factor affecting the observed spectral differences is the topology of the metal substrate, which may result in the induction of a cross-polarization effect. The performed analysis indicates that the C--C bond from the cyclohexyl group is oriented almost parallel to the metal surface. Conversely, the p- and s-polarized spectral variations suggest that the O=S---O angle is high enough to enable the simultaneous interaction of both oxygen atoms with the GNPs.

关键词： tip-enhanced infrared nanospectroscopy polarization modulation Y5 receptor antagonist gold nanoparticles adsorption

Analysis of the State of the Fine-Dispersive Precipitations in the Structure of High Strength Steel Weldox 1300 by means of Electron Diffraction

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Journal of Iron and Steel Research(International) 2011年第S1期18卷 135-142页

作者： Ozgowicz wojciech Labisz Krzysztof Division of Constructional and special materials Division of materials Processing Technology Management and Computer Techniques in Materials ScienceInstitute of Engineering Materials and BiomaterialsSilesian University of TechnologyKonarskiego St.18A44-100 GliwicePoland

The paper deals with investigations concerning the structure and state of dispersive precipitations of the phases consolidating in the high-strength steel Weldox 1300 after its high temperature thermomechanical *** use of transmission technique of electron microscopy (TEm) mainly the microstructure and morphology of martensite was analysed,as well as the state of dispersive precipitations of carbides and nitrides resulting from microadditives of such alloys as Nb,V,Ti or B,introduced into the *** revealed both a fine lathed and twin structure of the matrix of martensite in the investigated *** means of the method of electron diffraction particles of secondary strengthening phases of the m(C,N) type of a nanocrystalline size could be identified,and also non-metallic oxide inclusions,affecting the mechanism of strengthening of the investigated steel.

关键词： weldox 1300 HSLA steel thermomechanical treatment martensitic steel structural steel

Complete Oxidation of methane over Palladium Supported on Alumina modified with Calcium,Lanthanum,and Cerium Ions

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Journal of Natural Gas Chemistry 2007年第4期16卷 342-348页

作者： Beata Stasinska wojciech Gac Theophilos Ioannides Andrzej machocki University of maria Curie-Sklodowska Faculty of Chemistry Department of Chemical Technology3 Maria Curie-Sklodowska Square20-031 LublinPoland Foundation for Research & Technology-Hellas Institute of Chemical Engineering and High Temperature Chemical Processes(FORTH/ICE-HT) P.O.Box 1414PatrasGR-26500Greece

The activity and thermal stability of Pd/Al2O3 and Pd/（Al2O3＋mOx）（m=Ca, La, Ce） palladium catalysts in the reaction of complete oxidation of methane are presented in this study. The catalyst supports were prepared by sol-gel method and they were dried either conventionally or with supercritical carbon dioxide. Then they were impregnated with palladium nitrate solution. The catalysts with unmodified alumina had a high surface area. The activity and thermal stability of the aluminasupported catalyst was also very high. The introduction of calcium, lanthanum, or cerium oxide into alumina support caused a decrease of the surface area in the way dependent on the support precursor drying method. These modifiers decreased the activity of palladium catalysts, and they required higher temperatures for the complete oxidation of methane than unmodified Pd/Al2O3. The improvement of the palladium activity by lanthanum and cerium support modifier was observed only at low temperatures of the reaction.

关键词： catalytic oxidation of methane palladium catalysts alumina support modification lan- thanum modifier cerium modifier calcium modifier