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基于微流控技术的血清淀粉样蛋白A和C-反应蛋白检测试剂性能评价

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实验室检测

实验室检测 2024年第7期2卷 108-111页

作者：沃燕波阿里生物技术泰州有限公司

目的评价基于微流控技术的血清淀粉样蛋白A和C-反应蛋白检测试剂的分析性能，以判断其是否能满足临床需求。方法参照CLSI EP方案对检测试剂的精密度、准确度、线性范围和干扰试验等技术指标进行测定。结果血清淀粉样蛋白A检测试剂测... 详细信息

目的评价基于微流控技术的血清淀粉样蛋白A和C-反应蛋白检测试剂的分析性能，以判断其是否能满足临床需求。方法参照CLSI EP方案对检测试剂的精密度、准确度、线性范围和干扰试验等技术指标进行测定。结果血清淀粉样蛋白A检测试剂测定高、低浓度样本的批内变异系数分别为0.54%和1.79%，批间变异系数分别为0%和1.20%,C-反应蛋白检测试剂测定高、低浓度样本的批内变异系数分别为0.30%和2.28%，批间变异系数分别为0.17%和0%；两者方法学比对实验结果分别为Y=-0.0609+0.9871X,R2=0.9975和Y=0.3354+0.9857X,R2=0.9990，允许误差均大于预期偏差可信区间的上限，偏倚均可接受；血清淀粉样蛋白A检测试剂的线性范围为3～100.0 mg/L,C-反应蛋白检测试剂的线性范围为3～150.0 mg/L。结论基于微流控技术的血清淀粉样蛋白A和C-反应蛋白检测试剂分析性能可满足临床实验室需求。

关键词：血清淀粉样蛋白A C-反应蛋白微流控分析性能评价

民族地区教师数字素养的发展现状与提升路径——基于广西9市教师样本的实证分析

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民族教育研究 2024年第1期35卷 117-124页

作者：袁磊刘沃奇广西师范大学教育学部广西桂林541004 广西师范大学教育区块链与智能技术教育部重点实验室广西桂林541004

研究采用问卷调查与深度访谈的方法,系统评估广西壮族自治区9个市3588名基础教育阶段教师的数字素养,包括数字意识、数字技术知识与技能、数字教学应用能力、数字安全与社会责任以及数字专业发展等5个核心维度。研究结果显示,广西基础... 详细信息

研究采用问卷调查与深度访谈的方法,系统评估广西壮族自治区9个市3588名基础教育阶段教师的数字素养,包括数字意识、数字技术知识与技能、数字教学应用能力、数字安全与社会责任以及数字专业发展等5个核心维度。研究结果显示,广西基础教育教师的数字素养整体上呈现积极态势,但其发展呈现不平衡性。进一步分析发现,教师的数字素养随着教龄的增长而逐渐降低,且尽管数字时代城乡教育资源差距有所缩小,但这种不均衡依旧对教师数字素养产生显著影响。针对研究发现提出以下建议:完善教师培训体系,以适应数字化时代的需求;创新教研体系,增强教师的数字教学能力;畅通数字资源平台通道,确保教育资源的公平可及;构建文化共生的教育环境,以推动教师数字素养的均衡与全面发展。

关键词：民族地区教师教育教师数字素养教师专业发展城乡数字资源不均衡

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In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

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Nano Research 2024年第5期17卷 4379-4390页

作者： Yuzhou Chen Mo Chen Chengxuan Yu Huizhu Li Liman Sai Nguyen T.K.Thanh Yueming Wang Yan wo Jian Zhang Xing Yang Evgenii L.Guryev Andrei V.Zvyagin Hao De Min Tang Shiyi Chen Yunxia Li Yuefeng Hao Sijia Feng Jun Chen Sports Medicine Institute of Fudan University Department of Sports MedicineHuashan HospitalFudan UniversityShanghai 200040China Department of Orthopedic Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Bone and Joint Surgery Department of OrthopedicsRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Department of Physics Shanghai Normal UniversityShanghai 200234China Biophysics Group Department of Physics and AstronomyUniversity College LondonLondon WC1E 6BTUK UCL Healthcare Biomagnetic and Nanomaterials Laboratories London W1S 4BSUK Department of Anatomy and Physiology School of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Orthopedics Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou 215500China Institute of Biology and Biomedicine Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Mathematical Institute University of OxfordOxford OX26GGUK Institute of Natural Sciences School of MathematicsShanghai Jiao Tong UniversityShanghai 200240China

The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage *** this work,the second near-infrared(NIR-II)fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their *** delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)*** Keller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with *** results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.

关键词： macrophage cell therapy NIR-II in vivo imaging delayed therapy tendon healing

《民法典》背景下个人信息保护的司法考察与制度完善

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征信 2024年第5期42卷 31-42页

作者：沃耘乔鹏飞天津商业大学法学院天津市300134

大数据时代,为充分保障个人的信息安全,我国相继施行的《民法典》《个人信息保护法》对此作出了较为全面的规定。考察相关规则的司法适用,发现我国个人信息保护存在个人信息与隐私权界定不清、损害赔偿威慑作用不足、公益诉讼制度效用... 详细信息

大数据时代,为充分保障个人的信息安全,我国相继施行的《民法典》《个人信息保护法》对此作出了较为全面的规定。考察相关规则的司法适用,发现我国个人信息保护存在个人信息与隐私权界定不清、损害赔偿威慑作用不足、公益诉讼制度效用未充分发挥、“知情—同意”标准不一且流于形式等问题。为解决上述问题,可通过构建可识别的场景化模式界定个人信息,引入情境脉络完整性理论判定隐私,审慎引入惩罚性赔偿,明晰个人信息保护公益诉讼的适用条件,构建“国家—行业—平台”三层规则体系实现实质同意,全面保护个人信息。

关键词：个人信息保护隐私权惩罚性赔偿 “知情—同意” 《民法典》

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Evaluation of Agricultural Contamination Level by Heavy Metals and Pesticides in Sediments of Rivers and Water Bodies for Shrimp Fishing in the Lower Ouémé: Case of the Aguigadji, Ahlan and Sele Stations in Benin

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Advances in Materials Physics and Chemistry 2024年第2期14卷 25-46页

作者： Souradjou Orou Goura Fadéby Modeste Gouissi Armelle Sabine Yélignan Hounkpatin Wakili Bolatito Yessoufou Tayéwo Sylvain Biaou Nonvignon Martial Fassinou Laboratory of Ecology Health and Animal Production (LESPA) Faculty of Agronomy (FA) University of Parakou (UP) Parakou Bénin Pluridisciplanary Research Laboratory for Technical Education (LARPET) University of Sciences Technologies Engineering and Mathematics of Abomey (UNSTIM) Lokossa Bénin

This study assessed sediment contamination by heavy metals and pesticide active ingredients linked to chemical inputs used in agricultural activities in the lower Ouémé. Pesticide residues from the organochlorine, pyrethroid and organophosphorus families were investigated by gas chromatography, and heavy metals (Cd, Pb, As, Ni, Zn, Fe, Mg, Cr and Hg) by atomic absorption spectrophotometry. The metallic pollution indices, the contamination factor (CF) and the ecological risk index were calculated. The results revealed 8 active ingredients in the rainy season and 9 in the dry season. Glyphosate was the active ingredient with the highest concentration at all stations, 9.65 ± 0.84 mg/kg recorded in the dry season at the Aguigadji station. All glyphosate values were above the EQS. DDT, Atrazine and Endosulfan also showed high concentrations in the dry and rainy seasons. Emamectin, Abamectin and Lambda Cyhalothrin also showed high concentrations in the dry season at Aguigadji, Ahlan and Sele. Only glyphosate was recorded at the control station (Toho), but in very low concentrations. Lead showed the highest concentrations at all the stations, 265.96 ± 21.02 mg/Kg in the rainy season and 255.38 ± 79.09 mg/Kg in the dry season, all detected at the Aguigadji station and above the EQS. Zn, Ni, Fe, Cu and Cr were all representative in both the dry and rainy seasons at the contaminated stations. Manganese showed high concentrations in the rainy season. Pb showed very high contamination (FC ≥ 6) at the Aguigadji and Ahlan stations and significant contamination (3 ≤ FC 6) at the Sele station in both the rainy and dry seasons. Ni, Hg and Cd, showed either moderate or significant contamination at the contaminated stations. The risk values showed a considerable ecological Ri (190 ≤ Ri < 380) in the rainy season and a moderate ecological Ri (95 ≤ Ri < 190) in the dry season at these contaminated stations.

关键词： Pesticides Heavy Metals Sediments Shrimp Fishing Area Lower Ouémé

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“网络安全”概念的嬗变及其检视与重构

江苏警官学院学报 2024年第3期39卷 98-106页

作者：孟卧杰江苏警官学院现代警务研究中心

“网络安全”的核心内涵经历了网络系统安全时代、网络空间安全时代和网络信息安全时代的嬗变,实现了科学技术、互联网和网络信息三次视阈转换,相关研究也从信息科学技术领域延展到哲学及人文社科等领域。由此,在使用“网络安全”概念时... 详细信息

“网络安全”的核心内涵经历了网络系统安全时代、网络空间安全时代和网络信息安全时代的嬗变,实现了科学技术、互联网和网络信息三次视阈转换,相关研究也从信息科学技术领域延展到哲学及人文社科等领域。由此,在使用“网络安全”概念时,应重视稳定性与灵活性的统一,明确不同学科研究边界,根据不同范畴设定相应的法律原则、管理对象及调控方式。为实现“网络安全”目标,应按照总体国家安全观与法治要求,构建涵盖多主体行为的法律规范体系和网络安全保护制度:对于系统安全,强化技术防范与刑罚震慑;对于空间安全,设计科学有效的制度,重视公民基本权利保障,提高行政执法效率;对于信息安全,科学处理网络信息安全时代的民事、刑事和行政法律关系。

关键词：网络安全嬗变检视重构

Impact of Dewatering on the Spatio-Temporal Distribution of Benthic Macroinvertebrate Communities in the Okpara River, Benin

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Journal of Geoscience and Environment Protection 2024年第2期12卷 30-43页

作者： Sylvain Tayéwo Biaou Fadéby Modeste Gouissi Armelle Sabine Yélignan Hounkpatin Zoulkanerou Orou Piami Wakili Bolatito Yessoufou Souradjou Orou Goura Nonvignon Martial Fassinou Laboratory of Ecology Health and Animal Production (LESPA) Faculty of Agronomy (FA) University of Parakou (UP) Parakou Benin Pluridisciplanary Research Laboratory for Technical Education (LARPET) University of Sciences Technologies Engineering and Mathematics of Abomey (UNSTIM) Lokossa Benin

Surface watercourses are areas of very high ecological and heritage value. Macroinvertebrates are bioindicators of the health of aquatic ecosystems. The aim of this study was to assess the effects of dewatering and re-watering cycles on benthic macroinvertebrate (BMI) communities. Two data collections were carried out at two stations (Okpara 1 and Okpara 2) on the Okpara river before and after dewatering. Thus, 8 samples of benthic macroinvertebrates and 12 physico-chemical parameters (T°C, pH, Transparency, Depth, Conductivity, Dissolved Oxygen that were measured in situ, and BOD5, COD, NH4+, NO3-, NO2- and PO43-) were assayed in the laboratory. Canonical Correspondence Analysis (CCA) was used to match physico-chemical data to MIB families. Shannon and Piélou diversity indices were used to determine the effects of dewatering on MIBs. The increase in temperature values of pH, BOD5, COD, NH4+, NO3-, NO2- and PO43-, after re-watering indicates the effect of dewatering on the quality of Okpara aquatic ecosystems. The benthic macrofauna collected consisted of 62.42% insects, 0.65% crustaceans, 6.48% molluscs, 0.72% worms and 0.14% arachnids. Whereas after re-watering, 21.67% insects, 0% crustaceans, 0.22% molluscs, 7.56% worms and 0.29% arachnids were recorded. Insects, crustaceans and molluscs were more abundant before dewatering than after. This was revealed by low abundances and taxonomic richness, as well as low Shannon index values of samples collected after re-watering.

关键词： Dewatering Benthic Macroinvertebrates Impact Physico-Chemical Parameters Okpara Rivers Nord-Benin

长期预防性治疗结合按需治疗控制遗传性血管性水肿——2021版WAO/EAACI国际遗传性血管性水肿管理指南解读

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中华预防医学杂志 2024年第5期58卷 698-705页

作者：汪慧英郭胤仕姚我程雷浙江大学医学院附属第二医院变态反应科杭州310009 上海交通大学医学院附属仁济医院变态反应(过敏)科上海200001 南京医科大学第一附属医院江苏省人民医院耳鼻咽喉科/过敏诊疗中心南京210029

2021版世界过敏组织(WAO)/欧洲过敏与临床免疫学会(EAACI)国际遗传性血管性水肿(hereditary angioedema,HAE)管理指南主要以高质量的随机对照试验为依据,对HAE进行临床分型,就HAE的按需治疗、短期预防性治疗和长期预防性治疗药物给予分... 详细信息

2021版世界过敏组织(WAO)/欧洲过敏与临床免疫学会(EAACI)国际遗传性血管性水肿(hereditary angioedema,HAE)管理指南主要以高质量的随机对照试验为依据,对HAE进行临床分型,就HAE的按需治疗、短期预防性治疗和长期预防性治疗药物给予分级推荐,并针对不同HAE患病人群提供管理策略。本文主要对HAE的短期、长期预防性治疗和按需治疗部分进行解读,并补充最新临床证据,以期为HAE的长期管理提供参考。

关键词：遗传性血管性水肿按需治疗长期预防性治疗指南

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35岁以上肝豆状核变性患者的临床特征分析

临床肝胆病杂志 2024年第1期40卷 116-120页

作者：沃洪云康成为詹蕾蒲小兵四川大学华西公共卫生学院/华西第四医院老年保健与姑息医学系成都610041 四川大学华西公共卫生学院/华西第四医院骨科成都610041

目的总结年龄>35岁发病的肝豆状核变性(HLD)患者的临床特征。方法回顾性收集2018年4月—2023年4月四川大学华西公共卫生学院诊治的年龄>35岁发病的HLD患者的临床资料,根据HLD患者临床症状,分为混合型(n=13)、肝型(n=12)和脑型(n=5)。收... 详细信息

目的总结年龄>35岁发病的肝豆状核变性(HLD)患者的临床特征。方法回顾性收集2018年4月—2023年4月四川大学华西公共卫生学院诊治的年龄>35岁发病的HLD患者的临床资料,根据HLD患者临床症状,分为混合型(n=13)、肝型(n=12)和脑型(n=5)。收集病例的性别、临床表现、确诊年龄、确诊距首发症状时间、家族史等信息;实验室检查,血常规、肝肾功能、血清铜、血清铜蓝蛋白、尿铜、凝血功能等指标以及影像学检查等情况。符合正态分布的计量资料多组间比较采用单因素方差分析;不符合正态分布的计量资料多组间比较采用Kruskal-Wallis H检验。计数资料组间比较采用Fisher确切概率法。结果30例HLD患者中,男女比例3∶1,平均年龄(46.13±5.88)岁,角膜K-F环阳性者占43.33%,肝硬化患者高达66.67%。三组间球蛋白、白球比、ALT、凝血酶原时间、国际标准化比率、部分活化凝血活酶时间差异均有统计学意义(F值分别为5.893、4.513、4.424、5.029、5.248、4.942,P值均<0.05)。结论35岁以后确诊HLD的患者男性居多,以混合型和肝型为主,肝脏和凝血功能情况较差,对于该年龄段不明原因的肝功能异常及肝硬化,应尽早筛查血清铜蓝蛋白、尿铜等指标,并进行肝肾功能和凝血功能的监测。

关键词：肝豆状核变性诊断成年人