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Application of Cydia pomonella expr.ssed sequence tags： Identification and expr.ssion of thr.e gener.l odor.nt binding pr.teins in codling moth

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Insect Science 2013年第5期20卷 559-574页

作者： Stephen F. Gar.zynski Br.d S. Coates Thomas r. Unr.h Scott Schaeffer.Der.ck Jiwan tyson Koepke Amit Dhingr. USDA-Ar. Yakima Agricultural Research Laboratory Wapato WA 98951 USDA-Ar. Corn Insect and Crop Genetics Research Unit Genetics Laboratory Iowa State University Ames IA 50011 Depar.ment of Hor.icultur. and Landscape Ar.hitectur. Washington State University Pullman WA 99164 USA

The codling moth, Cydia pomonella, is one of the most impor.ant pests of pome fr.its in the wor.d, yet the molecular.genetics and the physiology of this insect r.main poor.y under.tood. A combined assembly of 8？341 expr.ssed sequence tags was gener.ted fr.m r.che 454 GS-FLX sequencing of eight tissue-specific cDNA libr.r.es. Putative chemosensor. pr.teins （12） and odor.nt binding pr.teins （OBPs）（18） wer. annotated, which included thr.e putative gener.l OBP （GOBP）, one mor. than typically r.por.ed for.other.Lepidopter.. To fur.her.char.cter.ze CpomGOBPs, we cloned cDNA copies of their.tr.nscr.pts and deter.ined their.expr.ssion patter.s in var.ous tissues. Cloning and sequencing of the 698？nt tr.nscr.pt for.CpomGOBP1 r.sulted in the pr.diction of a 163 amino acid coding r.gion, and subsequent r.-PCr.indicated that the tr.nscr.pts wer. mainly expr.ssed in antennae and mouthpar.s. The 1？289 nt （160 amino acid） CpomGOBP2 and the novel 702 nt （169 amino acid） CpomGOBP3 tr.nscr.pts ar. mainly expr.ssed in antennae, mouthpar.s, and female abdomen tips. These r.sults indicate that next gener.tion sequencing is useful for.the identification of novel tr.nscr.pts of inter.st, and that codling moth expr.sses a tr.nscr.pt encoding for.a new member.of the GOBP subfamily.

关键词： codling moth EST odor.nt binding pr.teins

Der.gulation of splicing factor. and br.ast cancer.development

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分子细胞生物学报:英文版 2015年第5期7卷 388-401页

作者： Mar.o Silipo Hannah Gautr.y Alison tyson-Capper Institute of Cellular.Medicine Faculty of Medical SciencesNewcastle UniversityNewcastle upon Tyne NE24HHUK

It is well known that many genes implicated in the development and pr.gr.ssion of br.ast cancer.under.o aber.ant alter.ative splicing events to pr.duce *** changes in alter.ative splicingcan ar.se fr.mmutations or.single-nucleotide polymor.hisms(SNPs)within the DNA sequences of cancer.r.lated genes,which can str.ngly affect the activity of splicing factor. and influence the splice site ***,it is impor.ant to note that absence of mutations is not sufficient to pr.vent misleading choices in splice site *** is nowincr.asing evidence to demonstr.te that the expr.ssion pr.file of ten splicing factor.(including Sr. and hnr.Ps)and eight r.A-binding pr.teins changes in br.ast cancer.cells compar.d with nor.al *** modifications str.ngly influence the alter.ative splicing patter. of many cancer.r.lated genes despite the absence of any detr.mental mutations within their.DNA ***,a compr.hensive assessment of the splicing factor.status in br.ast cancer.is impor.ant to pr.vide insights into the mechanisms that lead to br.ast cancer.development and *** most studies focus on mutations that affect alter.ative splicing in cancer.r.lated genes,this r.view focuses on splicing factor. and r.A-binding pr.teins that ar. themselves der.gulated in br.ast cancer.and implicated in cancer.r.lated alter.ative splicing events.

关键词： alter.ative splicing splicing factor. r.A-binding pr.teins br.ast cancer

EFFECT OF r.r. EAr.H ADDITIONS ON Fr.CTUr. Pr.PEr.IES OF 2.25Cr.1Mo STEELS

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Acta Metallur.ica Sinica(English Letter.) 1989年第1期2卷 63-68页

作者： W.r.tyson B.FAUCHEr Physical Metallur.y r.sear.h Labor.tor.es CANMETOttawaCanada

The inclusion par.meter.,fr.ctur. sur.ace mor.hology and void gr.wth char.cter.stics of ten- sile and fr.ctur. toughness specimens of 2.25Cr.1Mo steels with and without r.r.-ear.h (r.)additions have been investigated by quantitative metaltogr.phy(QTM),scanning elec- tr.n micr.scopy(SEM)and ener.y disper.ive spectr.scopy(EDS).Ther. is a substantially higher.density of inclusions in the r.-tr.ated steel,which has lower.values of fr.ctur. pr.per. ties including cr.tical values of COD and J integr.l(δ_c and J_(IC)),fr.ctur. str.in(ε_f) and Char.y V-notch ener.y(CVN).The fr.ctur. sur.ace of the r.-tr.ated steel compr.ses equiaxed dimples of diameter. compar.ble with its inclusion spacing,wher.as for.the non-r.-tr.ated steels,a wide r.nge of dimple sizes is found with aver.ge diameter.much smaller.than the cor.esponding inclusion *** investigation indicates that the lower.values of fr.ctur. pr.per.ies for.the steel with r. at r.om temper.tur. may be ascr.bed to its lar.e content of r.-containing inclusions.

关键词： Cr.Mo steel fr.ctur. pr.per.ies inclusion par.meter. fr.ctur. mor.hology

Modeling of a long sand-pack for.heavy cr.de oil thr.ugh depletion tests utilizing methane gas

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Petr.leum 2021年第2期7卷 188-198页

作者： Bashir.Busahmin r.ma r.o Kar.i Stephen tyson Mor.eza Jami Petr.leum&Chemical Engineer.ng Universiti Teknologi BruneiBrunei Darussalam

With the enor.ous incr.ase in the demand for.cr.de oil,and decr.ase in the r.sour.es of conventional oil r.ser.oir.,ther. is a gr.at need to under.tand heavy or.foamy oil-gas dr.ve mechanism to maximize the oil and gas *** analyze the r.al movement of non-viscous heavy oil flow,the char.cter.stic featur.s of the oil-gas mixtur. has to be estimated to for.cast the futur. potential supply fr.m a heavy oil *** impor.ant question in heavy oil flow under.solution gas dr.ve is whether.the behaviour.of depletion tests can be simulated to model the heavy oil flow *** main objective of this r.sear.h is to develop a r.liable numer.cal model for.modelling heavy oil flow calibr.ted with contr.lled solution gas dr.ve exper.ments,and that makes a novelty in this *** this paper.CMG-STAr. model which is capable of simulating solution gas dr.ve tests that matched the r.sear.h *** heavy oil r.cover. model can deter.ine the r.lative per.eability cur.es for.oil and gas in the dualphase system using Cor.y’s *** a depletion r.te of 0.0418 psi/min,the maximum cumulative oil and gas pr.duction was obser.ed to be 13,000 cm^(3)and 8500 cm^(3),*** r.sults fr.m the bottom hole pr.ssur. and the block pr.ssur. simulation r.ns indicate that the fluid pr.per.ies such as sur.ace tension plays a significant r.le in the gas bubble *** r.sults ar. pr.mising,and helps to under.tand the complex behaviour.of heavy oil r.ser.oir. and thus can impr.ve heavy oil r.cover..

关键词： Depletion r.te Simulation Gas fluid dual-phase Sand-pack Heavy oil r.ser.oir Methane gas

Allometr.c r.lationships between pr.mar. size measur.s and sapwood ar.a for.six common tr.e species in snow-dependent ecosystems in the Southwest United States

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Jour.al of For.str. r.sear.h 2020年第6期31卷 2171-2180页

作者： Bhaskar.Mitr. Shir.ey A.Papuga Mr.ss Alexander.tyson Lee Swetnam Nate Abr.mson School of Natur.l r.sour.es and Envir.nment University of ArizonaTucsonAZ 85721USA Depar.ment of Geology Wayne State UniversityDetroitMI 48202USA Labor.tor. of Tr.e-r.ng r.sear.h University of ArizonaTucsonAZ 85721USA BIO5 Institute University of ArizonaTucsonAZ 85721USA Depar.ment of Hydr.logy and Water.r.sour.es University of ArizonaTucsonAZ 85721USA

High-elevation,snow-dependent,semiar.d ecosystems acr.ss southwester. United States ar. expected to be vulner.ble to climate change,including dr.ught and fi r.,with implications for.var.ous aspects of the water.*** that end,much less is known about the dynamics of tr.nspir.tion,an impor.ant component of the water.cycle acr.ss this *** the individual-tr.e scale,tr.nspir.tion is estimated by scaling mean sap fl ux density by the hydr.active sapwood ar.a(SA).SA also r.mains a key factor.in eff ectively scaling individual tr.e water.use to stand *** acr.ss lar.e spatial scales is nor.ally established by r.lating SA of a few tr.es to pr.mar. size measur.s,e.g.,diameter.at br.ast height(DBH),tr.e height(H),or.canopy diameter.CD).Consider.ng the impor.ance of SA in scaling tr.nspir.tion,the pr.mar. objective of this study was ther.for. to establish six species-specifi c(aspen,maple,white fi r.ponder.sa pine,Douglas fi r.Englemann spr.ce)allometr.c r.lationships between SA and thr.e pr.mar. size measur.s(DBH,CD,or.H)acr.ss two high-elevation,snow-dependent,semiar.d ecosystems in New Mexico and *** on multiple statistical cr.ter.a(coeffi cient of deter.ination,index of agr.ement,Nash–Sutcliff e effi-ciency)and ease of measur.ment in the for.st,we identifi ed DBH as the pr.mar. independent var.able for.estimating SA acr.ss all *** on gr.up r.gr.ssion analysis,we found allometr.c r.lationships to be signifi cantly(pr.nt for.the same species(ponder.sa pine,Douglas-fi r.acr.ss diff er.nt ***,our.allometr.c r.lationships pr.vide a valuable database for.estimating tr.nspir.tion at diff er.nt spatial scales fr.m sap fl ow data in some of our.most vulner.ble ecosystems.

关键词： Allometr. Diameter.at br.ast height Mountain ecosystem Sapwood ar.a Southwester. USA

Liver.adipose tissue cr.sstalk in alcohol-associated liver.disease: The r.le of mTOr

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Liver.r.sear.h 2022年第4期6卷 227-237页

作者： Yssa r.dr.guez Jack Dunfield tyson r.der.que Hong-Min Ni Depar.ment of Phar.acology Toxicology and TherapeuticsUniversity of Kansas Medical CenterKansas CityKSUSA

Backgr.und:Alcohol-associated liver.disease(ALD)is a major.chr.nic liver.disease ar.und the wor.d without successful *** alcoholic hepatitis is one of the most sever. for.s of ALD with high mor.ality,which is often associated with binge *** dr.nking dysr.gulates lipid metabolism,incr.ases adipose tissue lipolysis,and induces liver.steatosis and adipose tissue *** ev-idence implicates that cr.sstalk of liver.and adipose tissue in the pathogenesis of *** tar.et of r.pamycin(mTOr.is a phosphatidylinositol 3-kinase(PI3K)-like ser.ne/thr.onine pr.tein kinase that r.gulates lipid metabolism,cell pr.lifer.tion and ***,the r.le of mTOr.in r.gulating adipose-liver.cr.sstalk in binge dr.nking-induced or.an damage r.mains ***:We gener.ted liver.specific and adipocyte-specific r.gulator.-associated pr.tein of mTOr.r.tor.knockout(r.tor.LKO and r.tor.AKO)as well as Mtor.knockout(Mtor.LKO and Mtor.AKO)mice,by cr.ssing r.tor.flox and Mtor.flox mice with albumin Cr. or.adiponectin Cr. mice,*** addition,we gener.ted liver.and adipocyte double deletion of r.tor.or.Mtor.Mtor.LAKO and r.tor.LAKO)*** knockout mice with their.matched wild-type litter.ates(r.tor.WT and Mtor.WT)wer. subjected to acute gavage of 7 g/kg ***:Mice with adipocyte deletion of r.tor.or.Mtor.developed hepatomegaly and adipose tissue *** gavage incr.ased liver.injur.,hepatic steatosis and inflammation in mouse liver. as demonstr.ted by elevated ser.m alanine aminotr.nsfer.se activities,incr.ased hepatic levels of tr.-glycer.de and incr.ased hepatic number. of CD68 positive macr.phages in mouse liver. after.alcohol *** injur. was fur.her.exacer.ated by deletion of adipocyte r.tor.or.*** adipokine ar.ay analysis r.vealed that incr.ased levels of pr.-inflammator. cytokines IL-6 and TNF a as well as chemokine MCP-1 following acute alcohol gavage in wild-type mice,which wer. fur.her.incr

关键词： Alcohol-associated liver.disease(ALD) Liver.injur. Mechanistic tar.et of r.pamycin(mTOr. Adipose atr.phy Adipokine Liver.adipose tissue cr.sstalk

Tr.nsfor.er. pr.teins pr.tect br.ast cancer.cells fr.m accumulating r.plication str.ss by ensur.ng pr.ductive splicing of checkpoint kinase 1

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Fr.ntier. of Chemical Science and Engineer.ng 2016年第2期10卷 186-195页

作者： Andr.w Best Kather.ne James Ger.ld Hysenaj Alison tyson-Capper.David J. Elliott Institute of Genetic Medicine Newcastle University Newcastle NE1 3BZ UK Inter.isciplinar. Computing and Complex BioSystems r.sear.h Gr.up and Centr. for.Bacter.al Cell Biology Newcastle University Newcastle NE1 3BZ UK Institute for.Cellular.Medicine Newcastle University Newcastle NE2 4HH UK

Incr.ased expr.ssion levels of the r.A splicing r.gulator.Tr.nsfor.er.fl （abbr.viated Tr.2fl） have been r.por.ed in sever.l types of cancer. r.cent wor. has r.vealed an intimate cr.ss-r.gulation between Tr.2fl and the highly similar.Tr.2a pr.tein in human br.ast cancer.cells, though these two pr.teins ar. encoded by separ.te genes cr.ated by a gene duplication that occur.ed over.500 million year. ago. This cr.ss-r.gulation involves splicing contr.l of a special class of exons, called poison exons. Down-r.gulation of Tr.2fl r.duces splicing inclu- sion of a poison exon in the mr.A encoding Tr.2a, ther.by up-r.gulating Tr.2a pr.tein expr.ssion. This buffer. any splicing changes that might be caused by individual depletion of Tr.2fl alone. Discover. of this cr.ss-r.gulation pathway, and its by-pass by joint deple- tion of both human Tr.2 pr.teins, r.vealed Tr.2 pr.teins ar. essential for.br.ast cancer.cell viability, and led to the identification of impor.ant tar.ets for.splicing contr.l. These exons include a cr.tical exon within the checkpoint kinase 1 （CHK1） gene that plays a cr.cial function in the pr.tection of cancer.cells fr.m r.plication str.ss. Br.ast cancer.cells depleted for.Tr.2 pr.teins have r.duced CHK1 pr.tein levels and accumulate DNA damage. These data suggest Tr.2 pr.teins and/or.their.splicing tar.ets as possible cancer.dr.g tar.ets.

关键词： r.A splicing gene expr.ssion br.ast cancer.DNA damage CHK1

稀土元素对2.25Cr.1Mo钢断裂性能的影响

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金属学报 1988年第4期24卷 282-287页

作者：洪友士 W.r.tyson B.FAUCHEr 北京中关村中国科学院力学研究所加拿大CANMET物理冶金实验室

本文用定量金相仪(QTM)和扫描电镜(SEM)测定含与不含稀土元素(r.)的2.25Cr.1Mo钢的夹杂物尺寸及分布;观察断口形态并测定延伸区宽度和韧窝尺度及分布;测定拉伸样品纵剖面上孔洞面密度与真应变的关系.研究表明,添加r.的试验钢中夹杂物密... 详细信息

本文用定量金相仪(QTM)和扫描电镜(SEM)测定含与不含稀土元素(r.)的2.25Cr.1Mo钢的夹杂物尺寸及分布;观察断口形态并测定延伸区宽度和韧窝尺度及分布;测定拉伸样品纵剖面上孔洞面密度与真应变的关系.研究表明,添加r.的试验钢中夹杂物密度较高,在较低的应变下沿夹杂物界面发生孔洞形核、长大和汇合,导致含r.的2.25Cr.1Mo钢较低的室温断裂性能.

关键词： 2.25Cr.1Mo钢断裂性能夹杂物

Per.or.ance of a quasi-steady model for.hover.ng hummingbir.s

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Theor.tical & Applied Mechanics Letter. 2015年第1期5卷 50-53页

作者： Jialei Song Haoxiang Luo tyson L.Hedr.ck Depar.ment of Mechanical Engineer.ng Vanderbilt University Depar.ment of Biology University North Carolina at Chapel Hill

A quasi-steady model descr.bing aer.dynamics of hover.ng r.by-thr.ated hummingbir.s is pr.sented to study extent of the low-or.er.model in r.pr.senting the flow physics of the bir. and also to separ.tely quantify the for.es fr.m the tr.nslational, r.tational, and acceler.tion effects. r.alistic wing kinematics ar. adopted and the model is calibr.ted against computational fluid dynamics （CFD） simulations of a cor. r.sponding r.volving-wing model. The r.sults show that the quasi-steady model is able to pr.dict over.ll lift pr.duction r.asonably well but fails to captur. detailed for.e oscillations. The downstr.ke-upstr.ke asymmetr. is consistent with that in the pr.vious CFD study. Fur.her.analysis shows that significant r.- tational for.e is nr.duced dur.ng mid-str.ke r.ther.than win~ r.ver.al.

关键词： Animal flightHummingbir. aer.dynamicsQuasi-steady model