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Hsa-miR-214-3p inhibits breast cancer cell growth and improves the tumor immune microenvironment by downregulating B7H3

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Oncology research 2025年第1期33卷 103-121页

作者： Yan Lu Kang Wang Yuanhong Peng Meng Chen Lin Zhong Luji Huang f U Cheng Xindan Sheng Xin Yang Manzhao Ouyang George A Calin Zhiwei He China-America Cancer Research Institute Guangdong Medical University Dongguan 523808 China. GCP Center Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China. Department of Gastrointestinal Surgery Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China. The Second School of Clinical Medicine Southern Medical University Guangzhou 510080 China. Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston TX 77030 USA. Department of Oncology Shunde Hospital Southern Medical University (The First People's Hospital of Shunde Foshan) Shunde 528300 China.

Background:Immune checkpoint inhibitors play an important role in the treatment of solid tumors, but the currently used immune checkpoint inhibitors targeting programmed cell death-1 (PD-1), programmed cell death ligand-1 (PD-L1), and cytotoxic T-lymphocyte antigen-4 (CTLA-4) show limited clinical efficacy in many breast cancers. B7H3 has been widely reported as an immunosuppressive molecule, but its immunological function in breast cancer patients remains unclear.Methods:We analyzed the expression of B7H3 in breast cancer samples using data from the Cancer Genome Atlas Program (TCGA) and the Gene Expression Omnibus (GEO) databases. MicroRNAs were selected using the TarBase, miRTarBase, and miRBase databases. The regulatory role of the microRNA hsa-miR-214-3p on B7H3 was investigated through dual-luciferase reporter assays, which identified the specific action sites of interaction. The expression levels of B7H3 and hsa-miR-214-3p in human breast cancer tissues and adjacent normal tissues were quantified using Western blotting and quantitative PCR (qPCR). experiments were performed to observe the effects of modulating the expression of B7H3 or hsa-miR-214-3p on breast cancer cell proliferation and apoptosis. Additionally, the regulatory impact of hsa-miR-214-3p on B7H3 was examined. Enzyme-linked immunosorbent assays (ELISA) and flow cytometry were employed to assess the effects of co-cultured breast cancer cells and normal human peripheral blood mononuclear cells (PBMCs) on immune cells and associated cytokines.Results:In breast cancer tissues, the expression level of B7H3 is inversely correlated with that of hsa-miR-214-3p, as well as with the regulatory effects on breast cancercell behavior. Hsa-miR-214-3p was found to inhibit breast cancer cell growth by downregulating B7H3. Importantly, our research identified, for the first time, two binding sites for hsa-miR-214-3p on the 3' UTR of B7H3, both of which exert similar effects independently. Co-culture experime

关键词： B7H3 Breast cancer Hsa-miR-214-3p Immunotherapy

Human T-cell immunity against the emerging and re-emerging viruses

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Science China(Life Sciences) 2017年第12期60卷 1307-1316页

作者： Min Zhao Hangjie Zhang Kefang Liu George f.Gao william J.Liu Research Network of lmmunity and Health(RN1H) Beijing Institutes of Life ScienceChinese Academy of SciencesBeijing 100101China Key Laboratory of Medical Virology and Viral Diseases Ministry of Health of People's Republic of ChinaNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China

Over the past decade, we have seen an alarming number of high-profile outbreaks of newly emerging and re-emerging *** outbreaks of avian influenza viruses, Middle East respiratory syndrome coronaviruses, Zika virus and Ebola virus present great threats to global health. Considering the pivotal role of host T-cell immunity in the alleviation of symptoms and the clearance of viruses in patients, there are three issues to be primarily concerned about T-cell immunity when a new virus emerges: first, does the population possess pre-existing T-cells against the new virus through previous infections of genetically relevant viruses; second, does a proper immune response arise in the patients to provide protection through an immunopathogenic effect; lastly, how long can the virus-specific immune memory persist. Herein, we summarize the current updates on the characteristics of human T-cell immunological responses against recently emerged or re-emerged viruses, and emphasize the necessity for timely investigation on the T-cell features of these viral diseases, which may provide beneficial recommendations for clinical diagnosis and vaccine development.

关键词： emerging viruses avian influenza H7N9 MERS-CoV Zika Ebola human immunity T-cell cross-reactivity

Variations in zonal fruit starch concentrations of apples – a developmental phenomenon or an indication of ripening?

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Horticulture Research 2015年第1期2卷 84-92页

作者： franziska C Doerflinger william B Miller Jacqueline f Nock Christopher B Watkins Horticulture Section School of Integrative Plant ScienceCornell UniversityIthaca14853NYUSA School of Agriculture Food&WineUniversity of AdelaideWaite CampusGlen Osmond5064South AustraliaAustralia

Patterns of starch hydrolysis in stem,equatorial,and calyx zones of‘Honeycrisp’and‘Empire’apples(Malus sylvestris(L.)Mill ***(Borkh.)Mansf.)during maturation and ripening,and in‘Gala’apples in response to propylene or 1-methylcyclopropene(1-MCP)treatments after harvest,were *** in zonal starch concentrations were found for‘Empire’and‘Gala’fruits,but not for‘Honeycrisp’.During maturation and ripening of‘Empire’,the concentration of starch was highest in the calyx end and lowest in the stem region.Differences in rates of starch hydrolysis among zones were not detected.‘Honeycrisp’and‘Empire’had the highest concentration of sorbitol in the calyx region,whereas it was highest in the stem-end region in‘Gala’.The distribution differences of glucose,fructose,and sucrose were similar in all three cultivars;higher fructose and glucose concentrations in the stem region,and higher sucrose concentrations in the calyx end of the *** treatment of‘Gala’with propylene did not affect the internal ethylene concentration of the fruit but 1-MCP markedly inhibited *** concentrations were highest in the calyx end but gradients of starch among zones were not changed by postharvest *** rate of hydrolysis was slowed by 1-MCP treatment,but was unaffected by *** treatments influenced sorbitol,glucose,and fructose *** of starch concentration among the zones did not confirm differences in ripening,but reflected its uneven distribution throughout the fruit during ***,measured differences in zonal starch are most likely related to starch accumulation during fruit development,rather than differences in rates of starch degradation during ripening.

关键词： cultivar fir starch

Natural and anthropogenic sources of lead, zinc, and nickel in sediments of Lake Izabal, Guatemala

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Journal of Environmental Sciences 2020年第10期32卷 117-126页

作者： Elisandra Hernandez Jonathan Obrist-farner Mark Brenner william f.Kenney Jason H.Curtis Edward Duarte Geosciences and Geological and Petroleum Engineering Department Missouri Uniuersity of Science and Technology.RollaMO 65409USA. Department of Geological Sciences University of FloridaGainesvilleFL 32611USA Land Use and Enuironmental Change Institute Uniuersity of FloridaGainesvilleFL 32611USA Escuela de Quimica Universidad de San Carlos de Guatemala01012Guatermala

Sediments in Lake Izabal,Guatemala,contain substantial lead (Pb),zinc (Zn),and nickel (Ni).The lack of historical data for heavy metal concentrations in the sediments makes it difficult to determine the sources or evaluate whether inputs of metals to the lake have changed through *** measured the relative abundances and concentrations of Pb,Zn,and Ni by X-Ray fluorescence core scanning and by Inductively Coupled Plasma Optical Emission Spectrometry in three sediment cores to explore stratigraphic distributions of metals in the lake *** amounts of Pb and Zn in the core taken near the Polochic Delta suggest that galena and sphalerite mining increased Pb and Zn delivery to Lake Izabal between ~1945 and 1965 *** up-core Ni increase in the core taken near a different mine on the north shore of Lake Izabal suggests that recent nickel mining operations led to an increase in Ni concentrations in the local sediments,but amounts in the other cores indicate that Ni is not widely distributed throughout the *** cores from Lake Izabal are reliable recorders of heavy metal input to the lake,and were measured to establish background metal levels,which would otherwise be *** of Pb,Zn,and Ni in older,pre-20th-century Lake Izabal sediments reflect input from natural erosion of *** results provide previously unavailable estimates of background metal concentrations in Lake Izabal before the onset of *** results are necessary for future monitoring related to mining contamination of the lake ecosystem.

关键词： Lake Izabal Heavy metals Lead contamination Anthropogenic impacts Mining Guatemala

Method for rapid on-site identification of VOCs

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Journal of Environmental Sciences 2009年第7期21卷 1005-1008页

作者： Justin D fair william f Bailey Robert A felty Amy E Gifford Benjamin Shultes Leslie H Volles Department of Chemistry University of Connecticut Storrs Connecticut 06269-3060 USA. INfICON Inc. 2 Technology Place East Syracuse New York 13057 USA

Rapid on-site identification of volatile organic compounds （VOCs） in ambient air is an important first step in remediation efforts. This study describes modification of a commercially available, portable GC/MS system and development of an analysis protocol for rapid （〈 3 min） sampling and identification of VOCs typically found at contaminated sites at the low ppbv level.

关键词： field analysis gas chromatograph mass spectrometer toxic industrial chemicals thermal desorption

Development and validation of SuperDEM for non-spherical particulate systems using a superquadric particle method

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Particuology 2022年第2期20卷 74-90页

作者： Xi Gao Jia Yu Ricardo J.f.Portal Jean-françois Dietiker Mehrdad Shahnam william A.Rogers National Energy Technology Laboratory MorgantownWV 26506USA Leidos Research Support Team MorgantownWV 26506USA Centro de Investigação em Modelação e Otimização de Sistemas Multifuncionais Instituto Superior de Engenharia de LisboaPolytechnic Institute of Lisbon1959-007 LisboaPortugal Instituto de Engenharia Mecânica–Instituto Superior Técnico Universidade de Lisboa1049-001 LisboaPortugal

This article presents the development and validation of the Superquadric Discrete Element Method(SuperDEM)for non-spherical particle simulation using a superquadric particle method in open-source CfD suite MfiX.A superquadric particle-particle contact algorithm with accelerating and stabilizing strategy was developed.A superquadric particle-arbitrary wall contact algorithm was developed,which enables the simulation in complex *** solver was validated by comparing with experimental data generated in this study or available in the *** include cylinder contacting with a wall,static packing of M&M chocolate candies in a cylindrical container,static packing of cylinders in a cylindrical container,dynamic angle of repose of cylinders in a rotating drum,and discharging of chocolate candies from a ***,MPI parallelization of the solver was implemented and the parallel performance of the solver using MPI was assessed through large-scale simulations of 1 million,10 million,and 100 million particles on up to 6800 cores,which demonstrates that the SuperDEM solver has great potential for industrial-scale systems simulation.

关键词： DEM Particle Non-spherical Superquadric Parallelization MfiX

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Writing & freedom

Writing & freedom

Complement-dependent neuroinflammation in spinal cord injury:from pathology to therapeutic implications

作者： william f. Myers.

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Neural Regeneration Research 2025年第5期20卷 1324-1335页

作者： Hassan Saad Bachar El Baba Ali Tfaily firas Kobeissy Juanmarco Gutierrez Gonzalez Daniel Refai Gerald R.Rodts Christian Mustroph David Gimbel Jonathan Grossberg Daniel L.Barrow Matthew f.Gary Ali M.Alawieh Department of Neurosurgery Emory University School of MedicineAtlantaGAUSA Department of Neurology Yale UniversityNew HavenCTUSA Center for Neurotrauma Multiomics&Biomarkers(CNMB)Morehouse School of MedicineAtlantaGAUSA

Spinal cord injury remains a major cause of disability in young adults,and beyond acute decompression and rehabilitation,there are no pharmacological treatments to limit the progression of injury and optimize recovery in this *** the thorough investigation of the complement system in triggering and propagating cerebral neuroinflammation,a similar role for complement in spinal neuroinflammation is a focus of ongoing *** this work,we survey the current literature investigating the role of complement in spinal cord injury including the sources of complement proteins,triggers of complement activation,and role of effector functions in the *** study relevant data demonstrating the different triggers of complement activation after spinal cord injury including direct binding to cellular debris,and or activation via antibody binding to damage-associated molecular *** effector functions of complement have been implicated in spinal cord injury,and we critically evaluate recent studies on the dual role of complement anaphylatoxins in spinal cord injury while emphasizing the lack of pathophysiological understanding of the role of opsonins in spinal cord *** this pathophysiological review,we systematically review the different translational approaches used in preclinical models of spinal cord injury and discuss the challenges for future translation into human *** review emphasizes the need for future studies to dissect the roles of different complement pathways in the pathology of spinal cord injury,to evaluate the phases of involvement of opsonins and anaphylatoxins,and to study the role of complement in white matter degeneration and regeneration using translational strategies to supplement genetic models.

关键词： complement neuroinflammation neuroplasticity regeneration spinal cord injury targeted therapy

Biogenic Amines as Biomarkers for Neurotoxicity

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Biomedical and Environmental Sciences 1991年第1期4卷 207-216页

作者： SYED f.ALI LOUIS W.CHANG william SLIKKER JR Division of Reproductive and Developmental Toxicology National Center for Toxicological Research JeffersonDepartment of Pharmacology and Toxicology Department of Biochemistry and Molecular BiologyDivision of Reproductive and Developmental Toxicology National Center for Toxicological Research JeffersonDivision of Reproductive and Developmental Toxicology National Center for Toxicological Research Jefferson Arkansas 72079-9502 Department of Pharmacology and Toxicology Department of Biochemistry and Molecular BiologyDepartment of Pharmacology University of Arkansas for Medical Sciences Little Rock Arkansas 72205Arkansas 72079-9502 Department of Pharmacology and Toxicology Department of Pediatrics Universtiy of Arkansas for Medical Sciences and Arkansas Children's Hospital Little Rock Arkansas 72205 Arkansas 72079-9502 Department of Pharmacology and Toxicology Department of Pediatrics Universtiy of Arkansas for Medical Sciences and Arkansas Children's Hospital Little Rock Arkansas 72205

There has been a surge of interest over the past several years in the use of neurochemical endpoints to contribute to our understanding of the mechanism of action of neurotoxicants. In our present presentation, two biogenic amine systems were selected as examples of biomarkers for neurotoxicity. To investigate these neurochemical endpoints, two prototype neurotoxicants were evaluated in experimental animals. One agent, reserpine, was used to assess developmental neurotoxicity and administered prenatally, while the other, MDMA, was used in the adult animal. The neurochemical biomarkers measured were dopamine, serotonin, and their metabolite (DOPAC and 5-HIAA) concentrations by HPLC/EC and dopamine receptor binding by radioligand receptor techniques. A review of the background, experimental design, and results are presented in this article. Our findings indicate that components of the biogenic amine systems can be used as sensitive neurochemical biomarkers of neurotoxicity. These neurochemical biomarkers can be correlated with neuropathological and behavioral biomarkers to aid in the understanding of mechanisms of neurotoxicity.

关键词： MDMA Biogenic Amines as Biomarkers for Neurotoxicity oral DOPAC