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Cocktails of defined chemical compounds:sufficient to induce totipotency in embryonic stem cells

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Signal Transduction and Targeted Therapy 2022年第10期7卷 3630-3631页

作者： wilfried A.kues Dharmendra Kumar Friedrich-Loeffler-Institut Federal Research Institute for Animal HealthBiotechnologyStem Cell Physiology31535 NeustadtGermany Animal Physiology and Reproduction Division ICAR-Central Institute for Research on BuffaloesHisarHaryana 125001India

In a recent work published in Nature,Hu et al.1 report that a cocktail of three small chemicals is sufficient to induce totipotency in murine embryonic stem(ES)*** this year,two other groups identified different mixtures of chemicals,which also seem to do this job,2,3 taken together this opens a new approach to capture totipotency in vitro,and to understand the underlying pathways and their interaction.

关键词： interaction. al. induce

Potential of transposon-mediated cellular reprogramming towards cell-based therapies

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World Journal of Stem Cells 2020年第7期12卷 527-544页

作者： Dharmendra Kumar Taruna Anand Thirumala R Talluri wilfried a kues Animal Physiology and Reproduction Division ICAR-Central Institute for Research on BuffaloesHisar 125001India NCVTC ICAR-National Research Centre on EquinesHisar 125001India Equine Production Campus ICAR-National Research Centre on EquinesBikaner 334001India Friedrich-Loeffler-Institut Institute of Farm Animal GeneticsDepartment of BiotechnologyMariensee 31535Germany

Induced pluripotent stem(iPS)cells present a seminal discovery in cell biology and promise to support innovative treatments of so far incurable *** translate iPS technology into clinical trials,the safety and stability of these reprogrammed cells needs to be *** recent years,different non-viral transposon systems have been developed for the induction of cellular pluripotency,and for the directed differentiation into desired cell *** this review,we summarize the current state of the art of different transposon systems in iPS-based cell therapies.

关键词： Transposons Induced pluripotent stem cells Clinical applications Cellular reprogramming Cell-based therapy Genetic correction

Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals

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World Journal of Stem Cells 2015年第2期7卷 315-328页

作者： Dharmendra Kumar Thirumala R Talluri Taruna Anand wilfried a kues Institute of Farm Animal Genetics Friedrich-Loeffler-Institute Animal Physiology and Reproduction Division Central Institute for Research on Buffaloes National Research Centre on Equines

Pluripotent stem cells are unspecialized cells withunlimited self-renewal, and they can be triggered to differentiate into desired specialized cell types. These features provide the basis for an unlimited cell source for innovative cell therapies. Pluripotent cells also allow to study developmental pathways, and to employ them or their differentiated cell derivatives in pharmaceutical testing and biotechnological applications. Via blastocyst complementation, pluripotent cells are a favoured tool for the generation of genetically modified mice. The recently established technology to generate an induced pluripotency status by ectopic co-expression of the transcription factors Oct4, Sox2, Klf4 and c-Myc allows to extending these applications to farm animal species, for which the derivation of genuine embryonic stem cells was not successful so far. Most induced pluripotent stem(i PS) cells are generated by retroviral or lentiviral transduction of reprogramming factors. Multiple viral integrations into the genome may cause insertional mutagenesis and may increase the risk of tumour formation. Non-integration methods have been reported to overcome the safety concerns associated with retro and lentiviral-derived i PS cells, such as transient expression of the reprogramming factors using episomal plasmids, and direct delivery of reprogramming m RNAs or proteins. In this review, we focus on the mechanisms of cellular reprogramming and current methods used to induce pluripotency. We also highlight problems associated with the generation of i PS cells. An increased understanding of the fundamental mechanisms underlying pluripotency and refining the methodology of i PS cell generation will have a profound impact on future development and application in regenerative medicine and reproductive biotechnology of farm animals.

关键词： Reprogramming Large animal models Stemness Chimera Germline transmission Inducedpluripotent stem cells Gene delivery

On-chip frequency combs and telecommunications signal processing meet quantum optics

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Frontiers of Optoelectronics 2018年第2期11卷 134-147页

作者： Christian REIMER Yanbing ZHANG Piotr ROZTOCKI Stefania SCIARA Luis Romero CORTES Mehedi ISLAM Bennet FISCHER Benjamin WETZEL Alfonso Carmelo CINO Sai Tak CHU Brent LITTLE David MOSS Lucia CASPANI Jose AZANA Michael kues Roberto MORANDOTTI Institut National de la Recherche Scientifique - Centre Energie Matrriaux et Telecommunications (INRS-EMT) 1650 Boulevard Lionel-Boulet Varennes Qurbec J3X 1S2 Canada Department of Energy Information Engineering and Mathematical Models University of Palermo Palermo Italy Department of Physics & Astronomy University of Sussex Falmer Brighton BN1 9QH UK Department of Physics and Material Science City University of Hong Kong Tat Chee Avenue Hong Kong China State Key Laboratory of Transient Optics and Photonics Xi'an Institute of Optics and Precision Mechanics Chinese Academy of Sciences Xi'an 710119 China Centre for Micro Photonics Swinburne University of Technology Hawthorn Victoria 3122 Australia Institute of Photonics Department of Physics University of Strathclyde Glasgow G1 1RD UK School of Engineering University of Glasgow Rankine Building Oakfield Avenue Glasgow G 12 8LT UK Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China Chengdu 610054 China National Research University of Information Technologies Mechanics and Optics St Petersburg 197101 Russia

Entangled optical quantum states are essential towards solving questions in fundamental physics and are at the heart of applications in quantum information science. For advancing the research and development of quantum technologies, practical access to the generation and manipulation of photon states carrying significant quantum resources is required. Recently, integrated photonics has become a leading platform for the compact and cost- efficient generation and processing of optical quantum states. Despite significant advances, most on-chip non- classical light sources are still limited to basic bi-photon systems formed by two-dimensional states （i.e., qubits）. An interesting approach beating large potential is the use of the time or frequency domain to enabled the scalable on- chip generation of complex states. In this manuscript, we review recent efforts in using on-chip optical frequency combs for quantum state generation and telecommunica- tions components for their coherent control. In particular, the generation of bi- and multi-photon entangled qubit states has been demonstrated, based on a discrete time domain approach. Moreover, the on-chip generation of high-dimensional entangled states （quDits） has recentlybeen realized, wherein the photons are created in a coherent superposition of multiple pure frequency modes. The time- and frequency-domain states formed with on-chip frequency comb sources were coherently manipulated via off-the-shelf telecommunications compo- nents. Our results suggest that microcavity-based entangled photon states and their coherent control using accessible telecommunication infrastructures can open up new venues for scalable quantum information science.

关键词： nonlinear optics quantum optics entangledphotons

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脊髓损伤女性产前、产时、产后保健管理指南

中国康复理论与实践 2020年第11期26卷 1256-1262页

作者： Sue Bertschy Markus Schmidt Kai Fiebag Ute Lange Simone kues Ines Kurze 刘根林(译) 周红俊(译) 李建军(译) 陈素文(译) 郑樱(译) 郝春霞(译) 张缨(译) 卫波(译) 王一吉(译) 康海琼(译) 逯晓蕾(译) 袁媛(译) 蒙倩茹(译) Swiss Paraplegic Research(SPF) NottwilSwitzerland Sana Clinics Duisburg DuisburgGermany Department of Neuro-urology BG Clinic HamburgHamburgGermany Department of Applied Heath Sciences University of Applied SciencesBochumGermany Department of Psychology BG Clinic HamburgHamburgGermany Department for Paraplegia and Neuro-urology Centre of Spinal Cord Injuries and DiseasesBad BerkaGermany 首都医科大学康复医学院北京市100068 中国康复研究中心北京博爱医院脊髓损伤康复科北京市100068 中国康复科学所北京市100068 首都医科大学附属北京妇产医院北京市100026

德国医学科学协会发布的女性脊髓损伤患者产前、产时、产后管理指南涉及从备孕到不同孕期及分娩等一系列问题的管理。鉴于目前针对这一复杂问题还缺少公众可及并循证的管理办法,这一新指南致力于建立脊髓损伤女性的妇科、产科和孕产期... 详细信息

德国医学科学协会发布的女性脊髓损伤患者产前、产时、产后管理指南涉及从备孕到不同孕期及分娩等一系列问题的管理。鉴于目前针对这一复杂问题还缺少公众可及并循证的管理办法,这一新指南致力于建立脊髓损伤女性的妇科、产科和孕产期保健管理标准。本指南的目标是为脊髓损伤女性产前、产时、产后的咨询和保健提供实用指导,填补该领域医疗服务的缺口,促进相关学科临床工作者的协作,激发深入的专业研究。

关键词：脊髓损伤女性怀孕分娩指南