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Genetic variation in the reduction of attractive floral traits of an annual tarweed in response to drought and apical damage

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Journal of Plant Ecology 2016年第5期9卷 629-635页

作者： wilfredo L.Gonzáles Lorena H.Suárez Ernesto Gianoli Laboratorio de Ecología Evolutiva LIDFacultad de Ciencias y FilosofíaUniversidad Peruana Cayetano HerediaLimaPerú Departamento de Ecología P.Universidad Católica de ChileSantiagoChile Departamento de Biología Universidad de La SerenaLa SerenaChile Departamento de Botánica Universidad de ConcepciónConcepciónChile

Aims Foliar herbivory and water stress may affect floral traits attractive to *** genotypes may differ in their responses to the interplay between these factors,and evolution of phenotypic plasticity could be expected,particularly in heterogeneous *** aimed at evaluating the effects of simulated herbivory and experimental drought on floral traits attractive to pollinators in genetic families of the annual tarweed Madia sativa,which inhabits heterogeneous environments in terms of water availability,herbivore abundance and pollinator *** In a greenhouse experiment with 15 inbred lines from a *** population located in central Chile(Mediterranean-type climate),we measured the effects of apical bud damage and reduced water availability on:number of ray florets per flower head,length of ray florets,flower head diameter,number of open flower heads per plant,flowering plant height and flowering *** Findings Apical damage and water shortage reduced phenotypic expression of floral traits attractive to pollinators via additive and non-additive *** in low water showed decreased height and had fewer and shorter ray florets,and fewer and smaller flower *** plants showed delayed flowering,were less tall,and showed shorter ray florets and smaller flower *** number of ray florets was reduced by damage only in the low water *** height,flowering time and number of flower heads showed among-family *** traits also showed genetic variation for plasticity to water *** floret length,flower head size and time to flowering showed genetic variation for plastic responses to apical *** in flowering time may allow *** to adjust to the increased aridity foreseen for its *** genetic variation for plastic responses was detected,conditions are given for evolutionary responses to selective forces acting on plastic *** suggest that the evolution o

关键词： flower genetic variation herbivory phenotypic plasticity water availability

Gain of transcription factor binding sites is associated to changes in the expression signature of human brain and testis and is correlated to genes with higher expression breadth

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Science China(Life Sciences) 2019年第4期62卷 526-534页

作者： Vandeclécio Lira da Silva André Mauricio Ribeiro dos Santos wilfredo Blanco Sandro José de Souza Bioinformatics Multidisciplinary Environment (BioME) Universidade Federal do Rio Grande do Norte (UFRN) Programa de Pós-Gradua??o em Genética e Biologia Molecular (PPGBM) Universidade Federal do Pará (UFPA) Department of Computer Science Universidade Estadual do Rio Grande do Norte (UERN) Brain Institute Universidade Federal do Rio Grande do Norte (UFRN)

The gain of transcription factor binding sites(TFBS) is believed to represent one of the major causes of biological *** we used strategies based on comparative genomics to identify 21,822 TFBS specific to the human lineage(TFBS-HS),when compared to chimpanzee and gorilla genomes. More than 40%(9,206) of these TFBS-HS are in the vicinity of 1,283 genes.A comparison of the expression pattern of these genes and the corresponding orthologs in chimpanzee and gorilla identified genes differentially expressed in human tissues. These genes show a more divergent expression pattern in the human testis and brain, suggesting a role for positive selection in the fixation of TFBS gains. Genes associated with TFBS-HS were enriched in gene ontology categories related to transcriptional regulation, signaling, differentiation/development and nervous ***, genes associated with TFBS-HS present a higher expression breadth when compared to genes in general. This biased distribution is due to a preferential gain of TFBS in genes with higher expression breadth rather than a shift in the expression pattern after the gain of TFBS.

关键词： TFBS transcript factor human evolution expression breadth

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委内瑞拉非法外汇风险识别及治理研究

委内瑞拉非法外汇风险识别及治理研究

作者： FLORES RAM(?)REZ wilfredo GREGORIO 北京交通大学

学位级别：硕士

在委内瑞拉,外汇管制措施始于2003年。委内瑞拉正面临着与攻击本国货币有关,以及国家公共收入的下降以及国际储备的减少的问题。出于上述原因,委内瑞拉政府决定在本国实行一种外汇制度,以期应对这种情况。为了实现这一目标,政府还设立... 详细信息

在委内瑞拉,外汇管制措施始于2003年。委内瑞拉正面临着与攻击本国货币有关,以及国家公共收入的下降以及国际储备的减少的问题。出于上述原因,委内瑞拉政府决定在本国实行一种外汇制度,以期应对这种情况。为了实现这一目标,政府还设立了名为国家对外贸易中心的机构,这机构负责指导国家的外汇政策。然而,委内瑞拉实施外汇管制引发民众从事非法外汇活动,这使得2003年实施外汇管制制度的目标难以实现。为了继续应对这种削弱委内瑞拉外汇管制制度的局面,2015年,国家对外贸易中心颁布了一项名为《第011号裁决》的新法规,这法规意指限制和调控用于支付海外消费的外汇。此外,委内瑞拉银行也参与了这一法规的实施过程,通过非法外汇活动系统检测客户行为,限制交易异常客户的外汇使用权。因此,通过本文会能够识别和防范外汇风险,从而衡量委内瑞拉银行对于控制和制止非法外汇活动的效力。同时,本文认为此项研究对委国意义重大,以期提供了一种有关如何减轻与变更控制系统相关的风险的新思考方式。

关键词：委内瑞拉风险及治理委内瑞拉银行非法外汇活动

SOC Turnover and Lime-CO<sub>2</sub>Evolution during Liming of an Acid Andisol and Ultisol

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Open Journal of Soil Science 2011年第2期1卷 49-53页

作者： wilfredo A. Dumale Jr. Tsuyoshi Miyazaki Kenta Hirai Taku Nishimura Department of Plant Science Nueva Vizcaya State University Bayombong Nueva Vizcaya Philippines Department of Biological and Environmental Engineering University of Tokyo Tokyo Japan.

Agricultural liming contributes significantly to atmospheric CO2 emission from soils but data on magnitude of lime- contributed CO2 in a wide range of acid soils are still few. Data on lime-contributed CO2 and SOC turnover for global acid soils are needed to estimate the potential contribution of agricultural liming to atmospheric CO2. Using Ca13CO3 (13C 99%) as lime and tracer, here we separated lime-contributed and SOC-originated CO2 evolution in an acidic Kuroboku Andisol from Tanashi, Tokyo Prefecture (35°44′ N, 139°32′ E) and Kunigami Mahji Ultisol of Nakijin, Okinawa Prefecture, Japan (26°38′ N, 127°58′ E). On the average, lime-CO2 was 76.84% (Kuroboku Andisol) and 66.36% (Kunigami Mahji Ultisol) of overall CO2 emission after 36 days. There was increased SOC turnover in all limed soils, confirming priming effect (PE) of liming. The calculated PE of lime (Kuroboku Andisol, 51.97% - 114.95%;Kunigami Mahji Ultisol, 10.13% - 35.61%) was entirely 12C turnover of stable soil organic carbon (SOC) since SMBC, a labile SOC pool, was suppressed by liming in our experiment. Our results confirmed that mineralization of lime-carbonates is the major source of CO2 emission from acid soils during agricultural liming. Liming can influence the size of CO2 evolution from agricultural ecosystems considering global extent of acid soils and current volume of lime utilization. We propose the inclusion of liming in simulating carbon dynamics in agricultural ecosystems.

关键词： Agricultural Liming Soil Organic Carbon SOC Turnover Andisol Ultisol

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气举喷射泵在马拉开波湖近海油田的应用

石油石化节能 2000年第3期 44-45页

作者： Juan Faustinelli wilfredo Briceno 徐春虹姚群良张军闯不详吉林油田钻采院吉林油田研究院

气举喷射泵系统在马拉开波湖近海油田试验获得了成功。本文着重介绍了该系统的工作原理、系统安装、主要优缺点等 ,并且对应用效果进行了重点分析。得出结论 ,该系统在气举采油方面是一个很好的增产新系统。

关键词：连续气举喷射泵高压气体能量转换增产

Inflammation, oxidative stress and renin angiotensin system in atherosclerosis

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World Journal of Biological Chemistry 2015年第3期6卷 209-217页

作者： Kazim Husain wilfredo hernandez Rais A Ansari Leon Ferder Department of Physiology Pharmacology and Toxicology Ponce School of Medicine Department of Biochemistry Ponce School of Medicine Department of Pharmaceutical Sciences College of Pharmacy Nova Southeastern University

Atherosclerosis is a chronic inflammatory disease associated with cardiovascular dysfunction including myocardial infarction, unstable angina, sudden cardiac death, stroke and peripheral thromboses. It has been predicted that atherosclerosis will be the primary cause of death in the world by 2020. Atherogenesis is initiated by endothelial injury due to oxidative stress associated with cardiovascular risk factors including diabetes mellitus, hypertension, cigarette smoking, dyslipidemia, obesity, and metabolic syndrome. The impairment of the endothelium associated with cardiovascular risk factors creates an imbalance between vasodilating and vasoconstricting factors, in particular, an increase in angiotensin Ⅱ(Ang Ⅱ) and a decrease in nitric oxide. The renin-angiotensin system(RAS), and its primary mediator Ang Ⅱ, also have a direct influence on the progression of the atherosclerotic process via effects on endothelial function, inflammation, fibrinolytic balance, and plaque stability. Anti-inflammatory agents [statins, secretory phospholipase A2 inhibitor, lipoprotein-associated phospholipase A2 inhibitor, 5-lipoxygenase activating protein, chemokine motif ligand-2, C-C chemokine motif receptor 2 pathway inhibitors, methotrexate, IL-1 pathway inhibitor and RAS inhibitors(angiotensin-converting enzyme inhibitors)], Ang Ⅱ receptor blockers and ranin inhibitors may slow inflammatory processes and disease progression. Several studies in human using anti-inflammatory agents and RAS inhibitors revealed vascular benefits and reduced progression of coronary atherosclerosis in patients with stable angina pectoris; decreased vascular inflammatory markers, improved common carotid intima-media thickness and plaque volume in patients with diagnosed atherosclerosis. Recent preclinical studies have demonstrated therapeutic efficacy of vitamin D analogs paricalcitol in Apo E-deficient atherosclerotic mice.

关键词： Atherosclerosis Renin-angiotensin system Inflammation Oxidants/antioxidants imbalance Antiinflammatory drugs Renin-angiotensin system blockers

Effect of paricalcitol and enalapril on renal inflammation/oxidative stress in atherosclerosis

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World Journal of Biological Chemistry 2015年第3期6卷 240-248页

作者： Kazim Husain Edu Suarez Angel Isidro wilfredo hernandez Leon Ferder Department of Physiology Pharmacology and Toxicology Ponce School of Medicine and Health Sciences Department of Biochemistry Ponce School of Medicine and Health Sciences

AIM: To investigate the protective effect of paricalcitol and enalapril on renal inflammation and oxidative stress in Apo E-knock out mice. METHODS: Animals treated for 4 mo as group(1) Apo E-knock out plus vehicle, group(2) Apo E-knock out plus paricalcitol(200 ng thrice a week),(3) Apo Eknock out plus enalapril(30 mg/L),(4) Apo E-knock out plus paricalcitol plus enalapril and(5) normal. Blood pressure(BP) was recorded using tail cuff method. The kidneys were isolated for biochemical assays using spectrophotometer and Western blot analyses. RESULTS: Apo E-deficient mice developed high BP(127 ± 3 mm Hg) and it was ameliorated by enalapril and enalapril plus paricalcitol treatments but not with paricalcitol alone. Renal malondialdehyde concentrations, p22 phox, manganese-superoxide dismutase, inducible nitric oxide synthase(NOS), monocyte chemoattractant protein-1, tumor necrosis factor-alpha and transforming growth factor-β1 levels significantly elevated but reduced glutathione, Cu Zn-SOD and e NOS levels significantly depleted in Apo E-knock out animals compared to normal. Administration of paricalcitol, enalapril and combined together ameliorated the renal inflammation and oxidative stress in Apo E-knock out animals. CONCLUSION: Paricalcitol and enalapril combo treatment ameliorates renal inflammation as well as oxidative stress in atherosclerotic animals.

关键词： Atherosclerosis Enalapril Paricalcitol Renal inflammation Oxidative stress

中国—东盟减贫合作:数字经济与社区主导型发展减贫的最佳实践

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中国-东盟研究 2020年第4期 84-97页

作者： [菲律宾]维弗雷多·帕·艾文达陈园园(译) 菲律宾大学迪里曼分校广西大学国际学院《中国-东盟研究》编辑部

本文试图探索如何将不断发展的数字经济与贫困社区的基层扶贫行动相结合,围绕菲律宾国情深入分析减贫、社区主导型发展和数字经济三大概念,探讨三者间的相互作用机制,以及如何将它们纳入政策指导的发展框架中。案例研究表明,菲律宾仍然... 详细信息

本文试图探索如何将不断发展的数字经济与贫困社区的基层扶贫行动相结合,围绕菲律宾国情深入分析减贫、社区主导型发展和数字经济三大概念,探讨三者间的相互作用机制,以及如何将它们纳入政策指导的发展框架中。案例研究表明,菲律宾仍然存在严重的数字鸿沟,道路不通、供电不稳、高新技术设备费用高昂以及缺乏使用和维护知识等问题限制贫困人口参与数字经济的能力,使其难以借助数字经济红利摆脱贫困,有碍于贫困人口的经济和社会地位提升。本文建议加强对贫困社区的基础设施支持,同时遵循渐进性及以人为本的基本原则进行国家发展政策制定。东盟国家需要加强协调,支持成员国采取具体行动,通过协作机制弥合数字鸿沟,推动区域经济和社会进一步发展。

关键词：数字经济社区主导型发展减贫社区发展以人为本的发展