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effects of developmental plasticity on heat tolerance may be mediated by changes in cell size in Drosophila melanogaster

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Insect Science 2020年第6期27卷 1244-1256页

作者： Nadja Verspagen Felix p.Leiva Irene M.Janssen wilco c.e.p.verberk Department of Animal ecology and physiology Institute for Water and Wetland ResearchRadboud UniversityNijmegenThe Netherlands Department of Human Genetics Radboud University Medical CenterRadboud Institute for Molecular Life SciencesNijmegenThe Netherlands

There is a growing interest in the physiology underpinning heat tolerance of ectotherms and their responses to the ongoing rise in ***,there is no consensus about the underlying physiological *** to''the maintain aerobic scope and regulate oxygen supply^hypothesis,responses to warming at different organizational levels contribute to the ability to safeguard energy metabolism via aerobic *** the cellular level,a decrease in cell size increases the capacity for the uptake of resources(e.g.,food and oxygen),but the maintenance of electrochemical gradients across cellular membranes implies greater energetic costs in small *** this study,we investigated how different rearing temperatures atTected cell size and heat tolerance in the fruit fly Drosophila *** tested the hypothesis that smallcr-celled flies are more tolerant to acute,intense heat stress whereas larger-celled flies are more tolerant to chronic,mild heat *** used the thermal tolerance landscape framework,which incorporates the intensity and duration of thermal *** temperatures strongly affected both cell size and survival *** found different effects of developmental plasticity on tolerance to either chronic or acute heat ***-reared flies had both smaller cells and exhibited higher survival times under acute,intense heat stress when compared to cold-reared ***,under chronic,mild heat stress,the situation was reversed and cold-reared flies,consisting of larger cells,showed better *** differences in heat tolerance could have resulted from direct effects of rearing temperature or they may be mediated by the correlated changes in cell ***,our results are consistent with the idea that a smaller cell size may confer tolerance to acute temperatures via enhanced oxygen supply,while a larger cell may confer greater tolerance to chronic and less intense heat stress via more efTicient use of resources.

关键词： cell size heat stress rearing temperature thermal death time thermal tolerance

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Seasonal changes on two different spatial scales： response of aquatic invertebrates to water body and microhabitat

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Insect Science 2005年第4期12卷 263-280页

作者： wilco c.e.p. verberk HeIN H. VAN KLeeF MARTIJN DIJKMAN pAUL VAN HOeK peTeR SpIeReNBURG HANS eSSeLINK Bargerveen Foundation Radboud University Nijmegen Department of environmental Studies Institute for Wetland and Water Research Radboud University Nijmegen Department of Animal ecology and ecophysiology Radboud University NijmegenNijmegen the Netherlands

Knowledge about the spatial and temporal scales of both habitat use and the functional significance of different adaptations is essential for an understanding of the population dynamics of invertebrate assemblages. This fundamental knowledge is not only interesting from an academic point of view, but is sorely lacking and needed in the field of restoration ecology. Many species are threatened due to degradation. Knowing what environmental conditions are needed dtLring the life cycle of these species is important in the design of restoration measures which aim to lift existing bottlenecks for threatened species. To assess the relative importance of water type and microhabitat in structuring the invertebrate assemblage during different seasons, invertebrates were sampled in three water bodies differing in trophic level and acidity. Different parts within a water body （microhabitats） were sampled separately and each water body was sampled in all four seasons. Results show that water body is an important factor structuring the invertebrate assemblage early in the season, whereas microhabitat became more important later in the season. Structural complexity of microhabitats was related to the type of locomotion employed by invertebrates. Seasonal differences could be related to population dynamics （reproduction, mortality）. Moreover, fluctuations in resource availability were expected to differ between the water bodies, with highest fluctuations in the eutrophic water body and with fluctuations becoming less predictable later in the season. This was confirmed by the data： species synchronization to pulses in food availability was strongest in the eutrophic water body. Moreover, synchronization was strongest in summer, while in autumn waters were invaded by dispersive species. Based on these results a synthesis is presented on the functioning of the different waters during the different seasons.

关键词： dispersal fluctuations in resource availability food substrate synchronization

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Induction of desiccation tolerance in desiccation sensitive citrus limon seeds

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Journal of Integrative plant Biology 2019年第5期61卷 624-638页

作者： Alexandre Marques Harm Nijveen charles Somi wilco Ligterink Henk Hilhorst Laboratory of plant physiology Wageningen University and ResearchWageningenThe Netherlands Bioinformatics Group Wageningen University and ResearchWageningenThe Netherlands

Many economically important perennial species bear recalcitrant seeds, including tea, coffee, cocoa, mango,citrus, rubber, oil palm and coconut. Orthodox seeds can be dried almost completely without losing viability, but so-called recalcitrant seeds have a very limited storage life and die upon drying below a higher critical moisture content than orthodox seeds. As a result, the development of longterm storage methods for recalcitrant seeds is *** this critical moisture content would be very valuable since dry seed storage is the safest, most convenient and cheapest method for conserving plant genetic ***, we have attempted to induce desiccation tolerance(DT) in the desiccation sensitive seeds of citrus limon. We show that DT can be induced by paclobutrazol(an inhibitor of gibberellin biosynthesis) and we studied its associated transcriptome to delineate the molecular mechanisms underlying this induction of DT. paclobutrazol not only interfered with gibberellin related gene expression but also caused extensive changes in expression of genes involved in the biosynthesis and signaling of other hormones. paclobutrazol induced a transcriptomic switch encompassing suppression of biotic-and induction of abiotic *** hypothesize that this is the main driver of the induction of DT by paclobutrazol in c. limon seeds.

关键词： economically important recalcitrant seeds paclobutrazol in c.limon seeds

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难加工材料的铣削和钻削加工

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航空制造技术 2013年第14期56卷 61-63页

作者： Teun Van Asten wilco van den Boogaard pr Nordberg 山高刀具公司

当前，减轻飞机重量这一目标正在促使航空航天工业采用越来越多的特种材料，如复合材料、钛合金和铬镍铁合金。相比普通材料，此类材料重量更轻、强度更高，但在加工方面，特别是铣削和钻削方面，它们各自拥有不同的加工难题。

关键词：难加工材料钻削加工铣削航空航天工业飞机重量特种材料复合材料镍铁合金

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Indications, Interpretation and clinical consequences of Tuberculin Skin Tests in Resource Limited Settings

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Journal of Tuberculosis Research 2021年第3期9卷 172-183页

作者： Fitzgerald A. Gopie Aabidien Hassankhan wilco c. W. R. Zijlmans Stephen G. S. Vreden Faculty of Medical Sciences Anton de Kom University of Suriname Paramaribo Suriname Academic Hospital paramaribo Paramaribo Suriname MoleMann Mental Health Suriname Paramaribo Suriname Department of paediatrics Diakonessenhuis Hospital Paramaribo Suriname Department of Internal Medicine Academic Hospital Paramaribo Paramaribo Suriname

Objective: to evaluate the policy of TST testing in Suriname. As there is no gold standard to diagnose latent tuberculosis infection (LTBI), the tuberculin skin test (TST) is used to diagnose LTBI. However, internationally, the cut-off values of the TST are not uniform and depend on local tuberculosis (TB) epidemiology and guidelines for test initiation. In Suriname, where currently several indications exist for TSTs, cut-off values are set at 5 mm or 10 mm, depending on the age and/or medical history of the patient. LTBI classification is performed by pulmonologists primarily based on the American Thoracic Society targeted TB testing guidelines. Method: retrospective analysis of outpatient TST data between 2011 and 2019 from Suriname’s sole pulmonary medicine clinic. Result: 1373 patients were evaluated. 590 patients were from the screening group of whom 253 had a positive TST result, 46 of whom were classified as LTBI. In the contact tracing group of 649 patients, 616 had a positive TST, 352 of whom were classified as LTBI. In the medical condition group of 134 patients, 96 had a positive TST, 38 of whom were classified as LTBI. Eventually, positive TST results were found for 965 tested patients: 436 patients were classified as LTBI and 529 non-LTBI patients were not prescribed chemoprophylaxis. None of the non-LTBI TST-positive patients were diagnosed with active TB, including 174 patients with a TST result of 15 mm or greater and in need of IPT, but not prescribed by jude="font-family:Verdana;">e="font-family:Verdana;">e="font-f

关键词： Tuberculin Skin Test cut-Off Values Guidelines Revision

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难加工材料的铣削和钻削加工

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工具技术 2014年第6期48卷 I0002-I0004页

作者： Teun Van Asten wilco van den Boogaard pr Nordberg Seco公司

为减轻飞机重量．航空航天工业越来越多地采用特种材料，例如复合材料、钛合金和铬镍铁合金。相比普通材料，这类材料重量更轻，强度更高。但在加工方面，特别是铣削和钻削时产生了不同的加工难题。在铣削和钻削加工中，航空航天制造商... 详细信息

为减轻飞机重量．航空航天工业越来越多地采用特种材料，例如复合材料、钛合金和铬镍铁合金。相比普通材料，这类材料重量更轻，强度更高。但在加工方面，特别是铣削和钻削时产生了不同的加工难题。在铣削和钻削加工中，航空航天制造商通常使用整体硬质合金刀具和（或）整体高速钢刀具，

关键词：难加工材料钻削加工铣削航空航天工业硬质合金刀具飞机重量高速钢刀具特种材料

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Neurologic Outcome of Surgical and conservative Treatment of Rheumatoid cervical Spine Subluxation:A Systematic Review and The Delphi Trial-I(RcT)2

Neurologic Outcome of Surgical and Conservative Treatment of...

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第六届国际脑血管病高峰论坛

作者： Jasper F.c.Wolfs Margreet Kloppenburg Michael G.Fehlings Maurits W.Van Tulder Marrten Boers wilco c.peul

Objective:Rheumatoid arthritis commonly involves the upper cervical spine and can cause significant neurologic morbidity and ***,there is no consensus on the optimal timing for surgical intervention:whether surgery should be performed prophylactically or once neurologic deficits have become ***:A systematic review of the literature

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