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Association of adiponectin gene polymorphisms with knee osteoarthritis

World Journal of Orthopedics 2017年第9期8卷 719-725页

作者： Dong Zhan Suthimon Thumtecho Aree Tanavalee Pongsak Yuktanandana wilai anomasiri Sittisak Honsawek Department of Biochemistry Faculty of Medicine Chulalongkorn University King Chulalongkorn Memorial Hospital Thai Red Cross Society Faculty of Medicine Chulalongkorn University King Chulalongkorn Memorial Hospital Thai Red Cross Society Vinai Parkpian Orthopaedic Research Center Department of Orthopaedics Faculty of Medicine Chulalongkorn University King Chulalongkorn Memorial Hospital Thai Red Cross Society

AIM To investigate the possible relationship of adiponectin(ADIPOQ) gene polymorphisms, plasma adiponectin, and the risk of knee osteoarthritis(OA).METHODS A total of 398 subjects, 202 knee OA patients and 196 healthy individuals, were enrolled in the case-control study. Genotyping at +45T/G(rs2241766) and +276G/T(rs1501299) loci was performed using polymerase chain reaction-restriction fragment length polymorphism. Plasma adiponectin levels were assessed using enzymelinked immunosorbent assay. OA severity was determined using the Kellgren-Lawrence(KL) grading *** No significant associations were observed in the genotype distributions and allele frequencies at two loci of +45T/G and +276G/T polymorphisms in the ADIPOQ betweenknee OA patients and control subjects. There was a significant association between genotype distribution of +276G/T polymorphism and KL grade 2, 3 or 4(P = 0.037, P = 0.046, P = 0.016, respectively). At +45T/G locus, the percentage of GG genotype was notably greater in control subjects(13.40%) compared with OA subjects(1.70%)(P = 0.023). Plasma adiponectin was markedly decreased in OA subjects compared with control subjects(P = 0.03). Likewise, circulating adiponectin in OA subjects was notably lesser than that in control subjects in GG genotype of +45T/G(P = 0.029) and +276G/T polymorphisms(P = 0.012).CONCLUSION Polymorphisms +45T/G and +276G/T of the ADIPOQ gene might not be responsible for OA susceptibility among Thais.

关键词： Adiponectin ADIPOQ Polymorphism Knee osteoarthritis Plasma

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Occurrence of antibiotics in typical pig farming and its wastewater treatment in Thailand

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Emerging Contaminants 2022年第1期8卷 21-29页

作者： Rathborey Chan Chart Chiemchaisri wilai Chiemchaisri Alongkot Boonsoongnern Phitsanu Tulayakul Department of Environmental Engineering Faculty of EngineeringKasetsart UniversityBangkok10900Thailand Department of Farm Resources and Production Medicine Faculty of Veterinary MedicineKasetsart UniversityNakhon Pathom73140Thailand Department of Veterinary Public Health Faculty of Veterinary MedicineKasetsart UniversityNakhon Pathom73140Thailand

This study investigated antibiotics in pig feed and drinking water and their excretion together with manure and wastewater at a typical pig farm in *** the 21 antibiotics analyzed,tetracyclines,macrolides,b-lactams,aminoglycosides,quinolones,sulfonamides,pleuromutilin,and florfenicol classes,were found as active ingredients in feed and drinking water and excreted together with pig *** them,doxycycline,tilmicosin,and amoxicillin were detected higher concentrations in wastewater(12.9e459.1 mg L^(-1)),sediment(0.074e0.62 mg kg^(-1)),and pig manure(0.45e4.1 mg kg^(-1)).During wastewater treatment,those major antibiotics were remaining in pond water and settled sludge up to 79.5 mg L-1 and 24.2 mg kg^(-1),*** normalized excretion mass of total antibiotics was determined as 11.2 mg head-1 day-1,contributed mainly from doxycycline(5.9 mg head-1 day-1),tilmicosin(2.8 mg head-1 day-1),and amoxicillin(2.2 mg head-1 day-1).Based on total pig production in Thailand,the excreted mass of antibiotics was estimated at 79.3 tons year-1.

关键词： Antibiotics Material flow analysis Pig farming Pond treatment Thailand

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Field investigation of antibiotic removal efficacies in different hospital wastewater treatment processes in Thailand

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Emerging Contaminants 2022年第1期8卷 329-339页

作者： wilai Chiemchaisri Chart Chiemchaisri Nutta Sangnarin Hamjinda Chisanupong Jeensalute Preeyanuch Buranapakdee Visanu Thamlikitkul Department of Environmental Engineering Faculty of EngineeringKasetsart UniversityPhaholyothin RdBangkok10900Thailand Department of Environmental Science Faculty of Science and TechnologyThammasat UniversityPathumthani12121Thailand Bureau of Environmental Health Department of HealthMinistry of Public HealthNonthaburi11000Thailand Division of Infectious Diseases and Tropical Medicine Department of MedicineFaculty of Medicine Siriraj HospitalMahidol UniversityBangkok10700Thailand

The removal efficacies of 19 antibiotics in different hospital wastewater treatment processes(WWTP)were investigated at various sizes of 60 hospitals across *** results showed slight differences in the total antibiotic removals(79e84%)among WWTP *** antibiotics loading to all WWTP were amoxicillin,ciprofloxacin,tetracycline ampicillin,and norfloxacin,*** and ampicillin were eliminated highly(90e99%)in every size of WWTP,while various efficacies showed for other antibiotics(0e93%).According to process comparison,activated sludge(CAS),oxidation ditch(OD),and sequencing batch reactor(SBR)systems showed similar removals of overall antibiotics(P>0.05).The attached growth systems such as submerged aerated fixed-film(SAFF)demonstrated relatively lower antibiotic *** natural processes such as aerated lagoon(AL),facultative pond(FP),and constructed wetland(CW)presented substantial biodegradation of some recalcitrant antibiotics such as fluoroquinolones,sulfamethoxazole,and ***-chlorination provided additional elimination of some antibiotics such as amoxicillin and ***,the top-five of antibiotics released into the water environment via discharged effluent are amoxicillin,tetracycline,ampicillin,ciprofloxacin,and imipenem,respectively,which mainly from the suspended growth systems.

关键词： Antibiotic Hospital Wastewater Treatment Process

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Environmental risk quotient of the antibiotic,phenotypic,and genotypic profiles for antibiotic resistance of Escherichia coli collected from manure and wastewater at swine farms in Prachinburi Province,Thailand

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Emerging Contaminants 2022年第1期8卷 340-350页

作者： Chalalai Rueanghiran Atchara Dawanpa Nayika Pinneum Arsooth Sanguankiat Chart Chiemchaisri wilai Chiemchaisri Watcharapong Sritumpawa Olarn Kijpreedaborisuthi Byeonghwa Jeon Phitsanu Tulayakul Department of Veterinary Public Health Faculty of Veterinary MedicineKasetsart UniversityKamphaeng Saen Campus73140Thailand Department of Environmental Engineering Faculty of EngineeringKasetsart University10900Thailand Bureau of Livestock Standards and Certification Department of Livestock Development10400Thailand Faculty of Veterinary Medicine Kasetsart University10900Thailand Environmental Health Science School of Public HealthUniversity of MinnesotaUSA55108 Prachinburi Provincial Livestock Office Department of Livestock Development25000Thailand

Currently,antimicrobial drug resistance is a problem that deserves thoughtful *** in the swine production industry,many pig farms tend to release wastewater into natural water sources,which villagers use for their *** study aimed to demonstrate the occurrence of phenotypic and genotypic resistance characteristics of ***,as well as antibiotic residues in manure and wastewater on swine farms in Prachinburi Province,*** were collected in 2020 and 2021 during the rainy and summer *** results of a questionnaire showed that farmers used antibiotics for disease treatment and prevention at a ratio of 2/4(50%).The most common antibiotic used was amoxicillin(4/4,100%),followed by Enrofloxacin(75%)and colistin(25%).The analysis of antibiotic residue in wastewater by Liquid Chromatography-Mass Spectrometry(LCMS,LC-MS)during the rainy season revealed that amoxicillin(54%)was the highest,followed by florfenicol(14%)and tiamulin(13%).While oxytetracycline(59%),amoxicillin(20%),and florfenicol(11%)were the highest in the *** study of Risk Quotient(RQ)indicated that water resources present a risk of antibiotic contamination by sulfonamides,trimethoprim,b-lactam,quinolones,and tetracycline at high levels(SRQ>1)during both *** minimal inhibitory concentrations(MICs)were determined by an antibiotic susceptibility test(AST),analyzed by microdilution technique,and VITEK®2 *** test result indicated that a total of 143 isolates were found in manure(45/143),pre-treatment(51/143),and final effluent(posttreatment,47/143).ESBL-positive resistance was detected at 13%.However,an immensely high percentage of antimicrobial resistance was found for ampicillin(90%),whereas imipenem did not show any drug resistance(0%).The average MIC value towards colistin in all samples was 2.71 mg/ml,while mcr1 was not found in any ***,MDR was expressed in *** at as high as 76.22%(109/143).The highest MDR pattern det

关键词： Escherichia coli Multidrug resistance Antibiotic residue LC-MS/MS Swine farm Pulse field gel electrophoresis(PFGE)

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家蚕雌蛹高温密闭对非着色死卵诱导效应

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国外农学（蚕业） 1992年第2期 42-43页

作者： San wilai 梁振中

以前我们曾报道将发育末期的家蚕雌蛹置于高温密闭条件下,羽化后与正常雄蛾交配,产下的卵基本不着色死亡,而置于高温和空气充足条件下,不发生此现象(Sugai等,1975)。这些现象对于生理学基础研究和家蚕卵的生产是重要的。本报告进一步研... 详细信息

以前我们曾报道将发育末期的家蚕雌蛹置于高温密闭条件下,羽化后与正常雄蛾交配,产下的卵基本不着色死亡,而置于高温和空气充足条件下,不发生此现象(Sugai等,1975)。这些现象对于生理学基础研究和家蚕卵的生产是重要的。本报告进一步研究了高温密闭环境对非着色死卵诱导效应,其重点为:空气容量与处理时间的关系,处理蛹的着色卵孵化率;非着色卵致死的细胞学佐证。

关键词：家蚕雌蛹高温死卵诱导

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无脚家蚕的不育性

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国外农学（蚕业） 1991年第4期 57-58页

作者： SAN wilai 梁振中

从欧洲种16号得到无脚蚕(ap)隐性突变体。基因纯合(ap/ap)时,胸脚完全退化,杂合型(f/ap)表现正常。无脚雌与正常蛾交配,或不产卵或产不受精卵,无脚雄表现正常生殖力(Hirobe,1944)。可是,有关资料不足以解释不育性原因。现发表为探测导... 详细信息

从欧洲种16号得到无脚蚕(ap)隐性突变体。基因纯合(ap/ap)时,胸脚完全退化,杂合型(f/ap)表现正常。无脚雌与正常蛾交配,或不产卵或产不受精卵,无脚雄表现正常生殖力(Hirobe,1944)。可是,有关资料不足以解释不育性原因。现发表为探测导致无脚蚕不育因素所进行的实验结果。

关键词：家蚕不育性无脚家蚕

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