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Molecular Epidemiology and Mechanism of Carbapenem-resistant Enterobacteriaceae in west china hospital

中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Cao He,Yi Xie,Mei Kang,Chuan-min Tao,Bo Xi e,Hong Fan,Lan-lan Wang Department of Laboratory Medicine,west china hospital,Sichuan University

Objectives To investigate the pathogenic characteristics in bloodstream infections(BSIs) in west china hospital. Methods A retrospective analysis of the microbiological results of blood culture and clinical data of BSIs from Jan 2009 to Mar 2010 was conducted at west china hospital. Results A total of 834 organisms were identified from the 826 episodes of the bloodstream infections during the study *** organisms were most

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Molecular Epidemiology and Mechanism of Carbapenem-resistant...

Effects of glucocorticoid and cysteinyl leukotriene 1 receptor antagonist on CD_(34)^+ hematopoietic cells in bone marrow of asthmatic mice

中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Ting-ting Wang,Chun-min Tao,Yi Xie,Hong-xia Ning,Dong-dong Li,Mei Kang, Liang Guo,Hui-li Chen,Hong Fan,Lan-lan Wang Department of Laboratory Medicine,west china hospital,Sichuan University

Objectives Carbapenems have a broad spectrum of activity against the drug-resistant ***,an increasing number of Enterobacteriaceae producing Klebsiella pneumoniae carbapenemases(KPC) were reported in the *** our study,we aimed to investigate the molecular epidemiology and mechanism of carbapenem-resistant Enterobacteriaceae isolates collected from west china hospital,Sichuan University.

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Chinese Medical Journal 2004年第4期117卷 592-597页

作者：毛辉殷凯生王曾礼李富宇Department of General Surgery west china hospital 张希龙刘春涛雷松Department of Tumor Biological Therapy west china hospital Department of Respiratory Medicine Department of Respiratory Medicine west china hospital

BACKGROUND: Corticosteroids remain the most effective therapy available for asthma. They have widespread effects on asthmatic airway inflammation. However, little is known about the effects of corticosteroids on the production of bone marrow inflammatory cells in asthma. This study observed the effects of glucocorticoid and cysteinyl leukotriene 1 receptor antagonist on CD34+ hematopoietic cells, so as to explore the possible effectiveness of a bone marrow-targeted anti-inflammatory strategy. METHODS: Balb/c mice were sensitized and challenged with ovalbumin (OVA) to establish an asthmatic model. For two consecutive weeks, asthmatic mice were challenged with OVA while being given either prednisone, montelukast, prednisone plus montelukast, or sterile saline solution. The mice were killed 24 hours after the last challenge with OVA, and bronchoalveolar lavage fluid (BALF), peripheral blood, and bone marrow were collected. Eosinophils in peripheral blood and BALF, and nucleated cells in BALF, peripheral blood, and bone marrow were counted. The percentages of CD34+ cells, CD4+ T lymphocytes and CD8+ T lymphocytes among nucleated cells in peripheral blood and bone marrow were counted by flow cytometry. Immunocytochemistry and in situ hybridization were employed to detect expression of CD34 and interleukin (IL)-5Ralpha mRNA (CD34+ IL-5Ralpha mRNA+ cells) among bone marrow hematopoietic cells. RESULTS: Compared with the sterile saline solution group, the number of eosinophils in BALF and peripheral blood, CD34+ cells in peripheral blood and bone marrow, and CD34+ IL-5Ralpha mRNA+ cells in bone marrow of mice from the prednisone and prednisone plus montelukast groups were significantly lower (P

关键词： Acetates Animals Antigens, CD34 Asthma Cell Count Hematopoietic Stem Cells Immunohistochemistry In Situ Hybridization Interleukin-5 Male Mice Mice, Inbred BALB C Prednisone Quinolines Research Support, Non-U.S. Gov't

Magnetic resonance imaging based volumetry:a primary approach to unravelling the brain

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Progress in Natural Science:Materials International 2007年第7期17卷 751-760页

作者： Huang Xiaoqi~(1,2) LüSu~1 Li Dongming~1 Gong Qiyong~(1,3**) (1.Huaxi MR Research Centre(HMRRC),and Department of Radiology,west china hospital/west china School of Medicine,Chengdu 610041,china 2.Department of Psychiatry,west china hospital/west china School of Medicine,Chengdu 610041,china 3.Division of Medical Imaging,Faculty of Medicine,University of Liverpool,United Kingdom) Huaxi MR Research Centre (HMRRC) and Department of Radiology West China Hospital / West China School of Medicine Chengdu 610041 China

Magnetic resonance(MR)imaging based volumetry is recognized as an important technique for studying the *** this review,two principle volumetric methods using high resolution MR images were introduced,namely the Cavalieri method and the voxel based morphometry(VBM).The Cavalieri method represents a manual technique that allows the volume of brain structures to be estimated efficiently with no systematic error or sampling bias,whereby the VBM represents an automated image analysis which involves the use of statistical parametric mapping of the MR imaging *** methods have been refined and applied extensively in recent neuro- science *** present paper aims to describe the development of methodologies and also to update the knowledge of their applica- tions in studying the normal and diseased brain.

关键词： MR imaging Cavalieri method voxel-based morphometry brain volumetry

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Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 356-361页

作者： Pei-Xian Chen and Lu-Nan Yan Division of Liver Transplantation,west china hospital,Sichuan University,Chengdu 610041,china Division of Liver Transplantation West China Hospital Sichuan University Chengdu 610041 China

BACKGROUND:Living donor liver transplantation (LDLT) has recently emerged as an effective therapeutic alternative for patients with end-stage liver *** the meantime,the health-related quality of life (HRQoL) of the donors is becoming better *** we aimed to review the current literature and summarize the effects of liver donation on the long-term HRQoL of living *** SOURCES:A literature search of PubMed using 'donors','living donor liver transplantation','health-related quality of life',and 'donation' was performed,and all the information was ***:The varied postoperative outcomes of liver donors are attributive to the different evaluation instruments *** the whole,donors experienced good long-term physical and mental well-being with a few complaining of compromised quality of life due to mild symptoms or psychiatric *** psychosocial dimension has received increasing attention with the vocational,interpersonal and financial impact of liver donation on donors mostly ***:Generally,donors have a good HRQoL after ***,to achieve an ideal donor outcome,further work is necessary to minimize the negative effects as well as to incorporate recent progress in regenerative medicine.

关键词： living donor liver transplantation donation health-related quality of life

The metabolic switch and its regulation in cancer cells

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Science china(Life Sciences) 2010年第8期53卷 942-958页

作者： ZHOU ShengTao, HUANG CanHua & WEI YuQuan State Key Laboratory of Biotherapy, west china hospital, Sichuan University, Chengdu 610041, china State Key Laboratory of Biotherapy West China Hospital Sichuan University

The primary features of cancer are maintained via intrinsically modified metabolic activity, which is characterized by enhanced nutrient supply, energy production, and biosynthetic activity to synthesize a variety of macromolecular components during each passage through the cell cycle. This metabolic shift in transformed cells, as compared with non-proliferating cells, in-volves aberrant activation of aerobic glycolysis, de novo lipid biosynthesis and glutamine-dependent anaplerosis to fuel robust cell growth and proliferation. Here, we discuss the unique metabolic characteristics of cancer, the constitutive regulation of metabolism through a variety of signal transduction pathways and/or enzymes involved in metabolic reprogramming in cancer cells, and their implications in cancer diagnosis and therapy.

关键词： aerobic glycolysis cancer de novo fatty acid synthesis glutaminolysis metabolism

Organ fibrosis inhibited by blocking transforming growth factor-β signaling via peroxisome proliferator-activated receptor γ agonists

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Hepatobiliary & Pancreatic Diseases International 2012年第5期11卷 467-478页

作者： Yi-Lei Deng, Xian-Ze Xiong, Nan-Sheng Cheng Department of General Surgery, west china hospital,Sichuan University, Chengdu 610041, china Department of General Surgery West China Hospital Sichuan University Chengdu 610041 Chengdu China

BACKGROUND:Organ fibrosis has been viewed as one of the major medical problems, which can lead to progressive dysfunction of the liver, lung, kidney, skin, heart, and eventually death of patients. Fibrosis is initiated by a variety of pathological, physiological, biochemical, and physical factors. Regardless of their different etiologies, they all share a common pathogenetic process: excessive activation of the key profibrotic cytokine, transforming growth factor-β (TGF-β). Peroxisome proliferator-activated receptor γ (PPARγ), a ligand-activated transcription factor of the nuclear receptor superfamily, has received particular attention in recent years, because the activation of PPARγ by both natural and synthetic agonists could effectively inhibit TGF-β-induced profibrotic effects in many organs. DATA SOURCES: The English-language medical databases, PubMed, Elsevier and SpringerLink were searched for articles on PPARγ, TGF-β, and fibrosis, and related topics. RESULTS: TGF-β is recognized as a key profibrotic cytokine. Excessive activation of TGF-β increases synthesis of extracellular matrix proteins and decreases their degradation, associated with a gradual destruction of normal tissue architecture and function, whereas PPARγ agonists inhibit TGF-β signal transduction and are effective antifibrogenic agents in many organs including the liver, lung, kidney, skin and heart. CONCLUSIONS: The main antifibrotic activity of PPARγ agonists is to suppress the TGF-β signaling pathway by so-called PPARγ-dependent effect. In addition, PPARγ agonists, especially 15d-PGJ2, also exert potentially antifibrotic activity independent of PPARγ activation. TGF-β1/Smads signaling not only plays many essential roles in multiple developmental processes, butalso forms cross-talk networks with other signal pathways, and their inhibition by PPARγ agonists certainly affects the cytokine networks and causes non-suspected side-effects. Anti-TGF-β therapies with PPARγ agonists may have to be car

关键词： PPAR gamma transforming growth factor-β fibrosis

A Novel Missense Mutation(L296Q)in Cholesteryl Ester Transfer Protein Gene Related to Coronary Heart Disease

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 33-36页

作者： Ke-Qin ZHENG, Si-Zhong ZHANG, Li ZHANG, De-Jia HUANG, Lin-Chuan LIAO, and Yi-Ping HOU ( Department of Medical Genetics, west china hospital Division of Human Morbid Genomics, Key Laboratory ofBiotherapy of Human Diseases of Ministry of Education, Sichuan University, Chengdu 610041, china Department of Cardiology, west china hospital, Sichuan University, Chengdu 610041, china School of Basic and Forensic Medicine, Sichuan University, Chengdu 610041, china) Department of Medical Genetics West China Hospita Division of Human Morbid Genomics Key Laboratory of Biotherapy of Human Diseases of Ministry of Education Sichuan University Chengdu 610041 Chin

Cholesteryl ester transfer protein (CETP) is a key participant in the reverse transport of cholesterol from the peripheral tissues to the liver. To understand the role that CETP gene plays in the pathogenesis of coronary heart disease (CHD) , the promoter region, all 16 exons and adjacent intronic regions of CETP gene were screened for single nucleotide polymorphisms (SNPs) in 203 CHD patients and 209 controls by a combination of PCR, denaturing high performance liquid chromatography (DHPLC), molecular cloning, and DNA sequencing. A novel missense mutation in the CETP gene was identified. This mutation (L296Q) was a T-to-A conversion at codon 296 of exon 10 which replaced the codon for leucine (CTG) with the codon for glutamine (CAG). Association study revealed that L296Q mutation was associated with CHD with a significantly higher mutant allele frequency in the CHD patients than that in the controls (0. 160 vs. 0.091,x2= 9.014, P = 0.003), and that the odds ratio for the development of CHD was 1.83 for

关键词： cholesteryl ester transfer protein gene (CETP gene) coronary heart disease (CHD) missense mutation plasma lipids single nucleoti.de polymorphism (SNP)

Interventional treatments for hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2006年第4期5卷 495-500页

作者： Yong-Song Guan and Yuan Liu Department of Radiology, west china hospital, Sichuan University, Chengdu 610041, china Department of Radiology West China Hospital Sichuan University Chengdu 610041 China. YongsongGuan@***

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most frequent primary malignant tumors in the world. Hepatic resection and liver transplantation are considered optimal for potential treatment of HCC. However, only 20% of HCCs can be surgically treated. And most of surgically-noneligible patients have to receive interventional managements including local ablation and transarterial chemoembolization (TACE). In this paper, we review the interventional treatments of HCC. DATA SOURCES: A literature search of PubMed database was conducted and research articles were reviewed. RESULTS: Percutaneous ethanol injection (PEI) is usually applied to small HCC for a complete necrosis. Radiofrequency ablation, an alternative to PEI, also causes tumor necrosis and needs fewer times of ablation. Other methods such as acetic acid injection, laser, microwave, etc have enriched local ablation for HCC. High intensity focus ultrasound (HIFU) is thought to be promising. TACE, another common modality, can improve the survival rate of patients with HCC. The newly developed embolic agents and adjuvant rAd-p53 gene therapy are well reported. CONCLUSIONS: Surgically-noneligible HCC can be treated with interventional procedures. Each method has its advantages and disadvantages. However, it is still pressing to develop ablative methods as well as new embolic agents for a better prognosis of HCC.

关键词： carcinoma, hepatocellular local ablative therapy transarterial chemoembolization