检索结果-南通市图书馆

Biomedical and Environmental Sciences 2015年第9期28卷 660-665页

作者： Faridah Yusof Atheer Awad Mehde wesen adel mehdi Raha Ahmed Raus Hamid Ghazali Azlina Abd Rahman Department of Biotechnology Engineering College of EngineeringInternational Islamic University Malaysia Department of Medical Analysis Health and Medical Technical College Department of Chemistry College of Sciences for WomenUniversity of Baghdad Department of Urology Hospital Tengku Ampuan Afzan Department of Pathology Hospital Tengku Ampuan Afzan

Objective Nephrolithiasis is one of the most common disorders of the urinary tract. The aim of this study was to examine a possible relationship between DNase Ⅰ/Ⅱ activity and E3 SUMO-protein ligase NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating kidney damage. Methods Sixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study. Their blood urea, creatinine, protein levels and DNase Ⅰ/Ⅱ activity levels were measured by spectrometry. Serum NSMCE2 levels were measured by ELISA. Blood was collected from patients of the government health clinics in Kuantan-Pahang and fulfilled the inclusion criteria. Results The result indicated that mean levels of sera NSMCE2 have a significantly increase（P〈0.01） in patients compared to control group. Compared with control subjects, activities and specific activities of serum DNase Ⅰ and Ⅱ were significantly elevated in nephrolithiasis patients（P〈0.01）. Conclusion This study suggests that an increase in serum concentrations of DNase Ⅰ/Ⅱ and E3 SUMO-protein ligase NSE2 level can be used as indicators for the diagnosis of kidney injury in patients with nephrolithiasis.

关键词： Nephrolithiasis DNase Ⅰ DNase Ⅱ E3 SUMO-protein ligase NSE2 NSMCE2

Levels of antioxidant enzymes and alkaline protease from pulp and peel of sunflower

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2017年第6期7卷 533-537页

作者： wesen adel mehdi Faridah Yusof Layla O.Farhan Atheer Awad Mehde Raha Ahmed Raus Department of Chemistry College of Sciences for WomenUniversity of Baghdad Department of Biotechnology Engineering College of EngineeringInternational Islamic University Malaysia Department of Technical Medical Analysis College of Health and Medical TechnologyMiddle Technical University

Objective:The activity of enzymes participating in the systems of antioxidant protection was assayed in the peel and pulp of *** essential roles of proteases in food stimulate research to find other sources of the enzyme especially from non-conventional *** the present work,we study several biochemical parameters in the pulp and peel of ***:Pulp and peel of sunflower was extracted,antioxidant enzymes and nonenzymatic antioxidant were *** protease was measured and purified from pulp in ***:High carbohydrate concentration,beta-carotene,catalase and ascorbate peroxidase activities,free radical scavenging capacity and free flavonoid content were observed in the peel of ***,MDA and ceruloplasmin activities were high in the pulp of ***:The present study concluded that peel in sunflower are strong radical scavengers and can be considered as good sources of natural antioxidants for medicinal and commercial *** analysis showed that protease activity was a significantly high in the pulp compared to the peel.

关键词： Pulp sunflower Antioxidant enzymes Free radical scavenging capacity Free flavonoid content Alkaline protease

The effect of renal stones on serum adenosine aminohydrolase and AMP-aminohydrolase in Malaysia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2015年第6期5卷 476-482页

作者： Faridah Yusof Atheer Awad Mehde wesen adel mehdi Hamid Ghazali Azlina Abd Rahman Department of Biotechnology Engineering College of Engineering International Islamic University Malaysia Department of Medical analysis Health and Medical Technical College Department of Chemistry College of Sciences for Women University of Baghdad Department of Urology Hospital Tengku Ampuan Afzan Department of Pathology Hospital Tengku Ampuan Afzan

Objective: To verify possible associations between adenosine aminohydrolase(ADA) and AMP-aminohydrolase(AMPDA) to E3 SUMO-protein ligase NSE2(NSMCE2) in patients with renal stones. And to isolate, purify and characterize ADA in patients with renal stones and healthy ***: A total of 60 renal stones patients and 50 control were enrolled in a case-control study. The blood urea, creatinine, uric acid, protein, albumin, ADA and AMPDA were measured by colorimetric tests. The serum NSMCE2 was measured by ***: Serum ADA, AMPDA and specii c activity of enzymes showed signii cant decrease(P < 0.05) in patients with renal stones compared to control group, mean levels of sera NSMCE2 and uric acid had a signii cant increase(P < 0.01 and P < 0.05, respectively) in patients compared to control ***: The present study suggests that ADA, AMP deaminase and NSMCE2 can be used as a indicator to monitor the DNA damage and inl ammation disorders in the patients with kidney stones.

关键词： Renal stone Adenosine aminohydrolase AMP-aminohydrolase Uric acid

Study the effect of kidney stones on serum xanthine oxidase, ecto-5'-nucleotidase activity and E3 SUMO-protein ligase NSE2(NSMCE2) in Malaysian individuals

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2015年第8期5卷 665-668页

Objective: To verify possible relations between 5'-nucleotidase, xanthine oxidase to E3 small ubiquitin-like modifier-protein ligase non structural maintenance of chromosomes elements 2 in sera patients with kidney stones and to evaluate the possibility of a new biomarker for the evaluation of kidney damage. Methods: A sixty patients with known kidney stones who appeared the government health clinics in Kuantan–Pahang and fifty apparently healthy were taken as control group. The 5'-nucleotidase,xanthine oxidase and other biochemical parameters were measured by colorimetric tests. The serum NSMCE2 were measured by enzyme linked immunosorbent ***: The mean serum xanthine oxidase [(39.98±19.70) IU/L] and ecto-5'-nucleotidase activity(40.03±9.53 IU/L) were significantly higher than the controls' levels of(18.04 ±6.26) and(16.06 ±4.61) IU/L respectively. There were 85.00% and 83.33%, of patients with kidney stones who had abnormal ecto-5'-nucleotidase activity and uric acid respectively while xanthine oxidase activity was less sensitive 58.33%.Conclusions: The present study suggests that the increase in serum of xanthine oxidase,ecto-5'-nucleotidase activities E3 small ubiquitin-like modifier-protein ligase NSE2 concentration can be used as biomarkers for diagnosis of kidney damage in patients with kidney stone,also in developments of change DNA damage and inflammation disorders in these patients.

关键词： Kidney stones Xanthine oxidase Ecto-5’-nucleotidase E3 SUMO-protein ligase NSE2

Serum E3 SUMO-protein ligase NSE2 level and peroxynitrite related to oxidative stress in nephrolithiasis patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 249-252页

作者： Atheer Awad Mehde Faridah Yusof wesen adel mehdi Raha Ahmed Raus Layla Othman Farhan Jwan Abdulmohsin Zainulabdeen Zaima Azira Zainal Abidin Hamid Ghazali Azlina Abd Rahman Department of Biotechnology Engineering College of EngineeringInternational Islamic University MalaysiaKuala Lumpur50728Malaysia Department of Technical Medical Analysis College of Health and Medical TechnologyMiddle Technical UniversityBaghdad10047Iraq Department of Chemistry College of Sciences for WomenUniversity of BaghdadBaghdad10071Iraq Department of Chemistry College of SciencesUniversity of BaghdadBaghdad10071Iraq College of Science International Islamic University MalaysiaKuantan25200Malaysia Department of Urology Hospital Tengku Ampuan AfzanKuantan25200Malaysia Department of Pathology Hospital Tengku Ampuan AfzanKuantan25200Malaysia

Objective: To prove probable relations between serum E3 SUMO-protein ligase NSE2(NSMCE2) concentration, peroxynitrite related to oxidative stress in nephrolithiasis ***: A total of 60 patients with nephrolithiasis and 50 healthy volunteers were involved in this study. Colorimetric method was used to detect blood urea, creatinine, uric acid, protein, albumin, total antioxidant status, total oxidant status, peroxynitrite, nitric oxide and oxidative stress index. Glutathione, NSMCE2 and superoxide dismutase were measured by ***: A significant increase in level of peroxynitrite, total oxidant status, NSMCE2 and oxidative stress index in patients was observed, while total antioxidant status and glutathione were significantly ***: The study concluded that serum NSMCE2 significantly correlated with peroxynitrite and oxidative stress in patients with nephrolithiasis.

关键词： Nephrolithiasis Peroxynitrite Nitric oxide E3 SUMO-protein ligase NSE2 Total oxidant status