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Food Science and Human Wellness 2024年第1期13卷 472-481页

作者： Wenjun Xue wenzhu zhao Sijia Wu Zhipeng Yu School of Food Science and Engineering Hainan UniversityHaikou 570228China College of Food Science and Engineering Bohai UniversityJinzhou 121013China Lab of Nutrition and Functional Food Jilin UniversityChangchun 130062China

The angiotensin-converting enzyme(ACE)inhibitory peptide NCW derived from Mizuhopecten yessoensis has been demonstrated to have significant in vivo anti-hypertensive effects,however,its anti-hypertensive mechanism is still not fully clarified.This study established a UPLC-Q-TRAP-MS/MS-based widely targeted kidney metabolomics approach to explore the changes of kidney metabolic profiles and to clarify the antihypertensive mechanism of peptide NCW in spontaneously hypertensive rats(SHRs).Multivariate statistical analysis indicated that the kidney metabolic profiles were clearly separated between the SHR-NCW and SHRUntreated groups.A total of 85 metabolites were differentially regulated,and 16 metabolites were identified as potential kidney biomarkers,e.g.,3-hydroxybutyrate,malonic acid,deoxycytidine,and L-aspartic acid.The peptide NCW might regulate kidney metabolic disorder of SHRs to alleviate hypertension by suppressing inflammation and improving nitric oxide production under the regulation of linoleic acid metabolism,folate related pathways,synthesis and degradation of ketone bodies,pyrimidine metabolism,β-alanine metabolism,and retinal metabolism.

关键词： ACE inhibitory peptide Kidney Mechanism Metabolomics Spontaneously hypertensive rats

Virtual screening,molecular docking and identification of umami peptides derived from Oncorhynchus mykiss

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Food Science and Human Wellness 2023年第1期12卷 89-93页

作者： wenzhu zhao Lijun Su Shitong Huo Zhipeng Yu Jianrong Li Jingbo Liu School of Food Science and Engineering Hainan UniversityHaikou 570228China College of Food Science and Engineering Bohai UniversityJinzhou 121013China Lab of Nutrition and Functional Food Jilin UniversityChangchun 130062China

Oncorhynchus mykiss is delicious and contains abundant flavor substances.However,few studies focused on umami peptides of O.mykiss.In the current work,umami peptides derived from O.mykiss were identified using virtual screening,molecular docking,and electronic tongue analysis.First,the O.mykiss protein was hydrolyzed using the PeptideCutter online enzymolysis program.Subsequently,water-soluble and toxicity screening were performed by Innovagen and ToxinPred software,respectively.The potential peptides were docked with umami receptor T1R1/T1R3.Furthermore,taste properties of potential peptides were validated by electronic tongue.Docking results suggested that the three tetrapeptide EANK,EEAK,and EMQK could enter the binding pocket in the T1R1 cavity,wherein Arg151,Asp147,Gln52,and Arg277 may play key roles in the production of umami taste.Electronic tongue results showed that the umami value of EANK,EEAK,and EMQK were stronger than monosodium glutamate.This work provides a new insight for the screening of umami peptides in O.mykiss.

关键词： Oncorhynchus mykiss Umami peptides Electronic tongue Molecular docking

Identifi cation of egg protein-derived peptides as xanthine oxidase inhibitors:virtual hydrolysis,molecular docking,and in vitro activity evaluation

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Food Science and Human Wellness 2022年第6期11卷 1591-1597页

作者： Zhipeng Yu Yaxin Cao Ruotong Kan Huizhuo Ji wenzhu zhao Sijia Wu Jingbo Liu David Shiuan School of Food Science and Engineering Hainan UniversityHaikou 570228China College of Food Science and Engineering Bohai UniversityJinzhou 121013China Lab of Nutrition and Functional Food Jilin UniversityChangchun 130062China Institute of Drug Discovery Technology Ningbo UniversityNingbo 315211China

The purpose of this study was to screen the xanthine oxidase(XO)inhibitory peptides from egg white proteins through virtual hydrolysis,in vitro activity validation,and molecular docking.The results demonstrated that tripeptide EEK from ovalbumin exhibited potent XO inhibitory activity with an IC50 value of 141μmol/L.The molecular docking results showed that tripeptide EEK bound with the active center of XO via 3 carbon hydrogen bond interactions,2 salt bridges,5 conventional hydrogen bond interactions,and 4 attractive charge interactions.The residues Glu802,Phe1009,and Arg880 may play key roles in the XO catalytic reaction.Especially,the key intermolecular forces of inhibiting XO activity may be special type of hydrogen bonds including carbon hydrogen bond interactions and attraction charge interactions.The novel tripeptide EEK is potential candidates for controlling hyperuricemia.

关键词： Egg protein-derived peptides Hyperuricemia Inhibitor mechanism Molecular docking Xanthine oxidase

Identification and molecular mechanism of angiotensin-converting enzyme inhibitory peptides from Larimichthys crocea titin

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Food Science and Human Wellness 2020年第3期9卷 257-263页

作者： Yue Fan Zhipeng Yu wenzhu zhao Long Ding Fuping Zheng Jianrong Li Jingbo Liu College of Food Science and Engineering Bohai UniversityJinzhou121013China Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Technology and Business University(BTBU)Beijing102488China College of Food Science and Engineering Northwest A&F UniversityYangling712100China Lab of Nutrition and Functional Food Jilin UniversityChangchun130062China

This study aimed to identify novel ACEI peptides from Larimichthys crocea titin using in silico approaches and to clarify the molecular interaction mechanism.The hydrolyzed peptides of titin were compared with known ACEI peptides in the AHTPDB and BIOPEP-UWM database.Furthermore,peptides were evaluated for their solubility,ADMET properties,ΔG(kcal/mol)values,and in vitro ACEI activity.Molecular mechanism of ACE-peptide was performed by molecular interactions and binding orientation study.The results revealed that IC50 values of Trp-Ala-Arg(WAR)and Trp-Gln-Arg(WQR)were(31.2±0.8)and(231.33±0.02)mol/L,respectively.The docking interactions result suggested that ACE-WAR and ACEWQR complexes have same binding site,including the residues LYS511,TYR520,TYR523,HIS353,and HIS513.Molecular docking of two tripeptides WAR and WQR with ACE studies predicted their binding site and clarified the interaction between ACE and its inhibitors.The molecular docking data are consistent with the ACE inhibitory activity of the studied peptides.The results showed that Larimichthys crocea titin may be a valuable source for developing nutraceutical food.

关键词： ACE peptide Identification In silico approaches ADMET Molecular docking

Biological evaluation and interaction mechanism of beta-site APP cleaving enzyme 1 inhibitory pentapeptide from egg albumin

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Food Science and Human Wellness 2020年第2期9卷 162-167页

作者： Zhipeng Yu Sijia Wu wenzhu zhao Long Ding David Shiuan Fuping Zheng Jianrong Li Jingbo Liu College of Food Science and Engineering National&Local Joint Engineering Research Center of StorageProcessing and Safety Control Technology for Fresh Agricultural and Aquatic ProductsBohai UniversityJinzhou121013PR China Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Technology and Business University(BTBU)Beijing102488PR China College of Food Science and Engineering Northwest A&F UniversityYangling712100PR China Lab of Nutrition and Functional Food Jilin UniversityChangchun130062PR China

Inhibition of beta-site APP cleaving enzyme1(BACE1)is one of the most promising therapeutic approaches for Alzheimer’s disease.To find natural products for the treatment of Alzheimer’s disease,absorption,distribution,metabolism,excretion and toxicity(ADMET)properties and in vitro BACE1 inhibitory activity of the peptides isolated from egg albumin were evaluated.Then,molecular docking and molecular dynamics simulation were used to explain the molecular mechanism of the interactions between BACE1 and peptides.The IC50 value of peptide KLPGF,with satisfactory ADMET properties,against BACE1 was(8.30±0.56)mmol/L.Molecular docking revealed that KLPGF contacted with the residues of BACE1’s active sites through twelve hydrogen bonds interactions,two hydrophobic interactions,one electrostatic interaction,and two Pi-cation interactions.The 5 ns molecular dynamics simulations confirmed that the structure of KLPGF with BACE1 was stable.Peptide KLPGF contacted the residues Lys321,Asp228,and Asn233 with stable hydrogen bonds.KLPGF may be a potential anti-BACE1 candidate.

关键词： Alzheimer’s disease BACE1 ADMET prediction Molecular docking Molecular dynamics KLPGF

Interaction mechanism of egg white-derived ACE inhibitory peptide TNGIIR with ACE and its effect on the expression of ACE and AT1 receptor

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Food Science and Human Wellness 2020年第1期9卷 52-57页

作者： Zhipeng Yu Hui Guo David Shiuan Chensi Xia wenzhu zhao Long Ding Fuping Zheng Jingbo Liu College of Food Science and Engineering Bohai UniversityJinzhou 121013PR China Beijing Advanced Innovation Center for Food Nutrition and Human Health Beijing Technology and Business University(BTBU)Beijing 102488PR China Lab of Nutrition and Functional Food Jilin UniversityChangchun 130062PR China

The egg white-derived hexapeptide TNGIIR inhibits angiotensin-converting enzyme(ACE)activity in vitro.In this work,molecular docking revealed that TNGIIR established hydrogen bonds with the S1(Ala 354),S2(Gln 281,His 513,Tyr 520 and Lys 511)and S1(Glu 162)pockets of ACE.In addition,the potential antihypertensive effect of the oral administration of TNGIIR in spontaneously hypertensive rats(SHR)was investigated,as was the effect of this peptide on the mRNA expression of ACE and angiotensin type 1(AT1)and type 2(AT2)receptors in renal tissue.The oral administration of TNGIIR(2,10 and 50 mg/kg)for up to four weeks did not reduce the blood pressure of SHR,in contrast to captopril(10 mg/kg,orally),but attenuated the mRNA expression of ACE and AT1 receptor(as did captopril).In contrast,both TNGIIR and captopril enhanced the expression of AT2 receptor mRNA.There was no change in the circulating concentration of angiotensin I,but a slight decrease(about 10%)was seen in the concentration of circulating angiotensin II with TNGIIR and captopril.

关键词： Egg white proteins ACE inhibitory peptide Gene expression Antihypertensive effect Molecular docking

滨海地区SMA-13沥青混合料低温抗裂性能

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洛阳理工学院学报（自然科学版） 2024年第1期34卷 27-34页

作者：胡琰琰吴金荣金情芬赵文竹安徽理工大学土木建筑学院安徽淮南232001 矿山地下工程教育部工程研究中心安徽淮南232001

选用聚酯纤维作为外掺剂,干法制备聚酯纤维掺量为0%、0.3%、0.35%、0.4%、0.45%、0.5%的SMA-13半圆试件,分别开展0、2、4、6、8次硫酸钠-干湿循环下半圆弯曲(SCB)试验,并以清水-干湿循环作为平行对照组。随着干湿循环次数的增加,盐-干... 详细信息

选用聚酯纤维作为外掺剂,干法制备聚酯纤维掺量为0%、0.3%、0.35%、0.4%、0.45%、0.5%的SMA-13半圆试件,分别开展0、2、4、6、8次硫酸钠-干湿循环下半圆弯曲(SCB)试验,并以清水-干湿循环作为平行对照组。随着干湿循环次数的增加,盐-干湿循环试件组和平行对照组试件的低温性能均降低;盐-干湿循环对试件的影响>清水-干湿循环对试件的影响,且清水-干湿循环后试件的低温性能劣化幅值较小;聚酯纤维掺量为0.4%的试件宏观评价指标断裂韧性KIC在各溶液干湿循环条件下均有较好表现,且实测数据均高于其他掺量纤维。结合SEM扫描电镜试验可知,掺量为0.4%的聚酯纤维能形成较好的纤维网状搭接结构,有利于试件整体性的提升和荷载的均匀传递。

关键词：沥青混合料低温抗裂性能 SCB试验 SEM扫描电镜试验

Commercial genetically modified corn and soybean are poised following pilot planting in China

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Molecular Plant 2024年第4期17卷 519-521页

作者： Mei Sun Suzhen Li wenzhu Yang Bowen zhao Youhua Wang Xiaoqing Liu Biotechnology Research Institute Chinese Academy of Agricultural ScienceBeijing 100081China Farmers'Daily Beijing 100025China

"Who will feed China?"This was a question raised by Lester Brown,the director of the World Watch Institute in the United States,in 1994.He predicted that China's grain production would decrease from 340 million tons in 1990 to 272 million tons by 2030,a 20%decrease,while China's population would reach 1.6 billion(Brown,1995).If the dietary structure was not improved,China would need to import 200-369 million tons of grain,equivalent to the global grain trade at the time.He projected the high possibility of a food crisis in China,which might further trigger a global food crisis.However,with the joint efforts of national agricultural researchers,represented by Yuan Longping,and the Chinese government,China's grain production has achieved 18 consecutive increases from 2004 to 2021.In 2022,grain production in China reached 633 million tons(NBS and Statistics,2022),while the population remained stable at 1.4 billion.As a result,China has achieved complete self-sufficiency in its staple food supply and has demonstrated to the international community through practical actions that the Chinese people can feed themselves.

关键词： planting soybean raised

Gene-expression analysis of single cells-nested polymerase chain reaction after laser microdissection

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World Journal of Gastroenterology 2003年第6期9卷 1337-1341页

作者： zhao-wenzhu BrunoSchmied Wen-HaoTang ArthurZimmermann MarkusBuechler HelmutFriess XinShi JoergKleeff DepartmentofGeneralSurgery UniversityofHeidelbergImNeuenheimerFeld110D-69120HeidelbergGermany DepartmentofGeneralSurgery ZhongdaHospitalSoutheastUniversityNanjing210009JiangsuProvinceP.R.China InstituteofPathology UniversityofBernInselspitalCH-3010BernSwitzerland

AIM: The structural and functional characteristics of cells are dependent on the specific gene expression profile. The ability to study and compare gene expression at the cellular level will therefore provide valuable insights into cell physiology and pathophysiology.METHODS: Individual cells were isolated from frozen colon tissue sections using laser microdissection. DNA as well as RNA were extracted, and total RNA was reversely transcribed to complementary DNA (cDNA). Both DNA and cDNA were analyzed by nested polymerase chain reaction (PCR). The quality of isolated DNA and RNA was satisfactory.RESULTS: Single cells were successfully microdissected using an ultraviolet laser micromanipulator. Nested PCR amplification products of DNA and cDNA of single cells could clearly be visualized by agarose gel electrophoresis.CONCLUSION: The combined use of laser microdissection and nested-PCR provides an opportunity to analyze gene expression in single cells. This method allows the analysis and identification of specific genes which are in volved inphysiological and pathophysiological processes in a complex of variable cell phenotypes.

关键词：基因表达聚合酶链反应细胞生理学病理生理学

食源性生物活性肽的胃肠道吸收障碍及跨膜转运研究进展

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中国食品学报 2022年第11期22卷 400-409页

作者：于志鹏薛文君武思佳赵文竹励建荣渤海大学食品科学与工程学院辽宁锦州121013 海南大学食品科学与工程学院海口570228 吉林大学食品科学与工程学院长春130062

食源性生物活性肽的低生物利用度在很大程度上阻碍了它们在保健食品开发中的应用。其中,胃肠道的消化降解及肠上皮的低渗透性是制约活性肽生物利用度的关键。构建安全、绿色、有效的策略对提升活性肽生物利用度意义重大,是活性肽领域亟... 详细信息

食源性生物活性肽的低生物利用度在很大程度上阻碍了它们在保健食品开发中的应用。其中,胃肠道的消化降解及肠上皮的低渗透性是制约活性肽生物利用度的关键。构建安全、绿色、有效的策略对提升活性肽生物利用度意义重大,是活性肽领域亟需克服的共性关键问题。本文综述生物活性肽的胃肠道吸收障碍、跨膜转运机制及提升活性肽生物利用度的最新策略,旨在为活性肽的深入研究和应用提供思路。

关键词：生物利用度稳定性黏液层肠上皮细胞递送系统