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Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions

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Molecular Plant 2015年第5期8卷 722-733页

作者： wenzhongxu wentao dai huili yan sheng li hongling shen yanshan chen hua xu yangyang sun zhenyan he mi ma Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China

In Arabidopsis, the nodulin 26-like intrinsic protein （NIP） subfamily of aquaporin proteins consists of nine members, five of which （NIP1;1, NIP1;2, NIP5;1, NIP6;1, and NIP7;1） were previously identified to be perme- able to arsenite. However, the roles of NIPs in the root-to-shoot translocation of arsenite in plants remain poorly understood. In this study, using reverse genetic strategies, Arabidopsis NIP3;1 was identified to play an important role in both the arsenic uptake and root-to-shoot distribution under arsenite stress condi- tions. The nip3;1 loss-of-function mutants displayed obvious improvements in arsenite tolerance for aboveground growth and accumulated less arsenic in shoots than those of the wild-type plants, whereas the nip3;1 nip1;1 double mutant showed strong arsenite tolerance and improved growth of both roots and shoots under arsenite stress conditions. A promoter-β-glucuronidase analysis revealed that NIP3; 1 was ex- pressed almost exclusively in roots （with the exception of the root tips）, and heterologous expression in the yeast Saccharomyces cerevisiae demonstrated that NIP3;1 was able to mediate arsenite transport. Taken together, our results suggest that NIP3;1 is involved in arsenite uptake and root-to-shoot translocation in Arabidopsis, probably as a passive and bidirectional arsenite transporter.

关键词： Arabidopsis, arsenite, NIP3 1, root-to-shoot translocation, tolerance

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Different roles of Arabidopsis NIP3;1 and NIP1;1 in root-to-shoot and root-tip distributions of arsenite

Different roles of Arabidopsis NIP3;1 and NIP1;1 in root-to-...

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从植物科学到农业发展——2012全国植物生物学大会

作者： Wenzhong xu,wentao dai,hongling shen,sheng li,yangyang sun,yanshan chen, huili yan,hua xu,zhenyan he,mi ma~* Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093.

In Arabidopsis,the roles of the NIPs in the arsenite distribution in plants remain poorly ***,we report that Arabidopsis NIP3;1 and NIP1;1 play different major roles in the shoot and root distributions of *** nip3;1 loss-of-function mutants displayed obvious improvements in high arsenite tolerance for aboveground growth,which was different from the tolerance to arsenite of the primary root growth of nip1;*** shoots of nip3;l accumulated less As than did those of the wild type under high arsenite conditions,indicating that NIP3;1 is involved in arsenite uptake and root-to-shoot *** with NIP1;1-promoter-β-glucuronidase constructs demonstrated that NIP1;1 was expressed in the whole root,particularly in the root-tip zone,and that the first exon/intron region was required for strong and specific expression of NIP1;1 in root-tip cells.A knockout mutation of NIP1;1 reduced arsenic accumulation in the root tips under arsenite conditions,suggesting that NIP1;1 plays a key role in the arsenite distribution at root-tip ***,a nip3;l nip1;1-double-mutant had a phenotype with strong arsenite tolerance that showed improved growth of both roots and shoots under arsenite *** together,we propose that NIP3;1 and NIP1;1 function as arsenite transporters for root-to-shoot translocation and root-tip distribution,respectively,in Arabidopsis.

关键词： Arabidopsis arsenite NIP1 1 NIP3 root-tip distribution root-to-shoot translocation

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