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Diagnosis of iridovirus in large yellow croaker by PCR

Acta Oceanologica Sinica 2003年第4期22卷 635-641页

作者： Chen Xinhua1. Wang Xiaowen2, Wu wenzhong3 1. Third Institute of Oceanography, State Oceanic Administration, Xiamen 361.05, China 2. School of Life Science, Xiamen University, Xiamen 361.05, China 3. Fujian Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China, Fuzhou 350000, China State Ocean Adm Inst Oceanog 3 Xiamen 361005 Peoples R China Xiamen Univ Sch Life Sci Xiamen 361005 Peoples R China Fujian Entry Exit Inspect & Quarantine Bureau Fuzhou 350000 Peoples R China

A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus (LYCIV) is described, which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen. Sequencing of LYCIV ATPase gene fragment showed it shared 1.0% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus (RSIV) and sea bass iridovirus (SBIV), suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone. The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes, the expected fragment was detected from spleen DNA samples of infected fishes, whereas no fragments were amplified from healthy fish spleen DNA, white spot syndrome baculoviruses (WSBV) DNA and pseudorabies virus (PRV) DNA. Detection limit of this method was 1.(-7) ng positive plasmid DNA containing target sequence, equal to about 1.0 virions. In the infected experiment, first positive detection (1.4) appeared at Day 3 post-infection, all fish (4/4) tested positive at Day 7, however obvious symptoms were observed at Day 8, so LYCIV infection could be detected prior to the appearance of obvious symptoms. These results indicate that this PCR method could be used for early, rapid and specific detection of LYCIV infection.

关键词： large yellow croaker (Pseudosciaena crocea) iridovirus ATpase gene pCR

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Highlights of mainstream solar cell efficiencies in 2023

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Frontiers in Energy 2024年第1期18卷 8-15页

作者： wenzhong SHEN Yixin ZHAO Feng LIU Institute of Solar Energy and Key Laboratory of Artificial Structures and Quantum Control(Ministry of Education)School of Physics and AstronomyShanghai Jiao Tong UniversityShanghai 200240China School of Environmental Science and Engineering and Frontiers Science Center for Transformative MoleculesShanghai Jiao Tong UniversityShanghai 200240China School of Chemistry and Chemical Engineering and Frontiers Science Center for Transformative MoleculesShanghai Jiao Tong UniversityShanghai 200240China

This article continues our highlights last two years[1.2]on the highest independently confirmed mainstream(silicon,perovskite,and organic)solar cell efficiencies in *** world record power conversion efficiency(PCE)of the single-junction silicon and perovskite/silicon tandem solar cells has reached over 27%and close to 34%,respectively,in *** have also witnessed the rapid mass-production development of the silicon passivating contact and silicon back contact solar cells,as well as further progress with perovskite solar cells(PSCs).

关键词： perovskite mainstream fir

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Multiphysical contact of functionally graded magneto-electroelastic material with imperfect bonding interface

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Friction 2024年第4期12卷 632-654页

作者： Yijin SUI Haibo ZHANG Jieliang ZHAO wenzhong WANG School of Mechanical Engineering Beijing Institute of TechnologyBeijing 100081China

Three-dimensional(3D)frictional contact model of functionally graded magneto-electro-elastic(FGMEE)material with a conducting spherical punch under electromagnetic fields is *** types of imperfect bonding interface of layers,dislocation-like interface and force-like interface,are *** response functions(FRFs)for multilayered MEE material with imperfect interface subjected to unit mechanical,electric,and magnetic loads are *** FRFs are used with the semi-analytical method(SAM)to solve present multiphysical contact *** present model is verified by comparing with literatures and the finite element method(FEM)and used to study the contact problem of FGMEE film imperfectly bonded on homogenous MEE half-space under electromagnetic *** studies are conducted to reveal the effects of imperfect interfaces and also film properties including gradient index and thickness.

关键词： frictional contact magneto-electro-elastic material imperfect interface functionally graded electromagnetic fields

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Improved Subsynchronous Oscillation Parameter Identification with Synchrophasor Based on Matrix Pencil Method in Power Systems

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Journal of Modern Power Systems and Clean Energy 2024年第1期12卷 22-33页

作者： Xiaoxue Zhang Fang Zhang wenzhong Gao Jinghan He the School of Electrical Engineering Beijing Jiaotong UniversityBeijing 100044China the Department of Electrical and Computer Engineering University of DenverDenverUSA

The subsynchronous oscillations(SSOs)related to renewable generation seriously affect the stability and safety of the power *** realize the dynamic monitoring of SSOs by utilizing the high computational efficiency and noise-resilient features of the matrix pencil method(MPM),this paper propos es an improved MPM-based parameter identification with syn *** MPM is enhanced by the angular frequency fitting equations based on the characteristic polynomial coeffi cients of the matrix pencil to ensure the accuracy of the identi fied parameters,since the existing eigenvalue solution of the MPM ignores the angular frequency conjugation constraints of the two fundamental modes and two oscillation ***,the identification and recovery of bad data are proposed by uti lizing the difference in temporal continuity of the synchropha sors before and after noise *** proposed parameter identification is verified with synthetic,simulated,and actual measured phase measurement unit(PMU)*** with the existing MPM,the improved MPM achieves better accuracy for parameter identification of each component in SSOs,better real-time performance,and significantly reduces the effect of bad data.

关键词： Subsynchronous oscillations(SSOs) synchrophasor parameter identification matrix pencil method bad data

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A passive sampler for synchronously measuring inorganic and organic pollutants in sediment porewater:Configuration and field application

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Journal of Environmental Sciences 2024年第2期136卷 201-212页

作者： Pei Lei Mingying Chen Nan Rong wenzhong Tang Hong Zhang School of Environment Nanjing Normal UniversityNanjing 210023China State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China South China Institute of Environmental Sciences Ministry of Ecology and EnvironmentGuangzhou 510530China State Environmental Protection Key Laboratory of Water Environmental Simulation and Pollution Control Guangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China

In situ measurement of multiple pollutants coexisting in sediment porewater is an essential step in comprehensively assessing the bioavailability and risk of pollutants,but to date,this needs to be better *** this study,a passive sampler,consisting of an“I-shaped”supporting frame and inorganic/organic sampling units,incorporating equilibrium dialysis theory and kinetic/equilibrium sorption principle,was developed for the synchronous measurement of inorganic(e.g.,phosphorus and metal(loid)s)and organic pollutants(e.g.,parent and substituted PAHs).The equilibrium time and sampling rates were explored in laboratory tests to support in situ *** of pollutants in porewater within a vertical resolution of centimeters,i.e.,1.cm and 2 cm for inorganic and organic pollutants,respectively,were obtained by field deployment of the sampler for further estimation of diffusive fluxes across the sediment-water *** results suggested that the role of sediments for a specific pollutant may change(e.g.,from“sink”to“source”)during the sampling *** study demonstrated the feasibility of synchronous measurement of inorganic and organic pollutants in sediment porewater by the passive *** addition,it provided new insight for further investigation into the combined pollution effects of various pollutants in sediments.

关键词： Passive sampling Bioavailability Polycyclic aromatic hydrocarbons Combined pollution Diffusive flux

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An oases dynamics model and its application in the Manas oasis

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Journal of Geographical Sciences 2002年第3期12卷 348-356页

作者： CHEN Zhengjiang, SHI wenzhong, HUI Yanghe(1. Dept. of Urban and Resource, Northwest University, Xi’an 71.069, China 2. Dept. of LandSurvey and Geo-information, The Hong Kong Polytechnic University, Hong Kong, China) Dept. of Urban and Resource Northwest University Xi'an 710069 Dept. of LandSurvey and Geo-information The Hong Kong Polytechnic University 香港

This study applies theories of systems and system dynamics to ecological economic systems in the oasis environment, with the intention of finding the basic characteristics of the oasis environment and its feedback structures. This study explores the inner mechanisms of economic development in the oasis environment and its relationship to policy variables. This model is applied to economic development in the Manas oasis of the Xinjiang Uygur Autonomous Region of China. System dynamics method starts from studying feedback structure within the system study and then searches for the best solution through identifying the right “policy point” and through model simulation in the computer. It embodies the cream of “systems cybernetics theory”. This study is justified because the nature of the oasis system and the simulation results are scientifically sound and practically feasible.

关键词： oasis dynamic modeling Manas oasis

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Sn-fused perylene diimides: Synthesis, mechanism, and properties

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Chinese Chemical Letters 2024年第10期35卷 244-248页

作者： wenzhong Zhang Zirui Yan Lingcheng Chen Yi Xiao State Key Laboratory of Fine Chemicals Dalian University of TechnologyDalian 116024China

The first example of metal Sn-fused perylene diimides(PDI)derivative(Sn-PDI)was designed,synthesized,and *** obtain this type compound,a simple one-pot synthesis,named stannylative cycloaddition reaction,has been successfully developed via a palladium-based catalyst *** novel mechanism exhibits that the reaction experiences oxidative addition,Pd-cyclization,stannylation,Pd-Sn-cyclization,and reductive elimination processes *** stannylative cycloaddition does realize uniqueσ-πhyperconjugation effect and therefore significantly influencing on the photophysical,electrochemical and excited state *** with those of PDI,both of the absorption and fluorescence spectra of Sn-PDI display large red-shifts over 20 *** electron energy levels of Sn-PDI have changed with an uncommon *** Sn-PDI gives a considerably raised highest occupied molecular orbital(HOMO)level of-6.00 eV More importantly,the singlet excitons of Sn-PDI could efficiently intersystem cross(ISC)into triplet state with a long lifetime of 1..8μs,which is far longer than that(4.4 ns)of PDI.

关键词： Functional dyes Perylene diimides Organometallic heterocyclics Hyperconjugation Triplet state

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A robust triaxial localization method of AE source using refraction path

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International Journal of Mining Science and Technology 2024年第4期34卷 521-530页

作者： Yichao Rui Jie Chen Jiongkun Chen Jiadong Qiu Zilong Zhou wenzhong Wang Jinyang Fan State Key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing 400044China School of Resources and Safety Engineering Chongqing UniversityChongqing 400044China School of Resources Environment and Safety Engineering University of South ChinaHengyang 421001China School of Resources and Safety Engineering Central South UniversityChangsha 410083China Coal Geological Exploration Institute of Gansu Lanzhou 730030China

Acoustic emission(AE)localization algorithms based on homogeneous media or single-velocity are less accurate when applied to the triaxial localization *** the end,a robust triaxial localization method of AE source using refraction path is ***,the control equation of the refraction path is established according to the sensor coordinates and arrival ***,considering the influence of time-difference-of-arrival(TDOA)errors,the residual of the governing equation is calculated to estimate the equation ***,the refraction points in different directions are solved using Snell’s law and orthogonal ***,the source coordinates are iteratively solved by weighted correction *** feasibility and accuracy of the proposed method are verified by pencil-lead breaking *** simulation results show that the new method is almost unaffected by the refraction ratio,and always holds more stable and accurate positioning performance than the traditional method under different ratios and scales of TDOA outliers.

关键词： Acoustic emission(AE) Source localization Robust method Refraction paths Weight estimations

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Source apportionment and specific-source-site risk of quinolone antibiotics for effluent-receiving urban rivers and groundwater in a city, China

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Journal of Environmental Sciences 2024年第10期144卷 185-198页

作者： Yu Zhao Yuanmeng Song Lulu Zhang Bo Zhao Mengqi Lu Jiansheng Cui wenzhong Tang State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of ScienceBeijing 100085China College of Environment Science and Engineering Hebei University of Science and TechnologyShijiazhuang 050000China University of Chinese Academy of Sciences Beijing 100049China

There is a large surface-groundwater exchange downstream ofwastewater treatment plants(WWTPs),and antibiotics upstream may influence sites downstream of ***,samples from 9 effluent-receiving urban rivers(ERURs)and 1. groundwater sites were collected in Shijiazhuang City in December 2020 and April *** ERURs,8 out of 1. target quinolone antibiotics(QNs)were detected,and the total concentration of QNs in December and April were 1.0.6-4,398 ng/L and 8.02–2,476 ng/L,*** groundwater,all target QNs were detected,and the total QNs concentration was 1.09–23.03 ng/L for December and 4.54–1.0.3 ng/L for *** distribution of QNs was dissimilar between ERURs and *** QN concentrations were weakly correlated with land use types in the *** results of a positive matrix factorization model(PMF)indicated four potential sources of QNs in both ERURs and groundwater,and WWTP effluents were the main source of *** December to April,the contribution of WWTP effluents and agricultural emissions increased,while livestock activities *** value decomposition(SVD)results showed that the spatial variation of most QNs was mainly contributed by sites downstream(7.09%-88.86%)of ***,a new method that combined the results of SVD and PMF was developed for a specific-source-site risk quotient(SRQ),and the SRQ for QNs was at high level,especially for the sites downstream of *** temporal variation,the SRQ for WWTP effluents,aquaculture,and agricultural emissions ***,in order to control the antibiotic pollution,more attention should be paid to WWTP effluents,aquaculture,and agricultural emission sources for the benefit of sites downstream of WWTPs.

关键词： Source apportionment Singular value decomposition Positive matrix factorization model Quinolone antibiotics(QNs) Specific-source-site risk quotient(SRQ) WWTP effluents

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Continuous Fe^(3+)releasing enabled complete reconstruction of Ni-based MOF for promoted water oxidation in industrial alkali

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Science China Chemistry 2024年第9期67卷 2949-2957页

作者： Zhaojian Wang Wei Zhang wenzhong Huang Ruohan Yu Changliang Chen Liqiang Mai Liang Zhou State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Sanya Science and Education Innovation Park Wuhan University of TechnologySanya 572000China Hubei Longzhong Laboratory Wuhan University of Technology(Xiangyang Demonstration Zone)Xiangyang 441000China

Most oxygen evolution reaction(OER)electrocatalysts show poor stability under industrial alkaline conditions(20–30 wt.%KOH).Therefore,it is essential to develop stable,efficient,and low-cost OER catalysts for industrial water ***,we present a straightforward approach for the complete electrochemical reconstruction of Ni-BDC,a Ni-based metal-organic framework,for *** method involves the continuous release of Fe^(3+)from Fe foam counter electrode in a high-concentration(6.0 M,25 wt.%)KOH *** continuously Fe^(3+)releasing not only realizes in situ Fe^(3+)doping,but also introduces abundant defects in the obtained catalyst during cyclic voltammetry activation,thereby accelerating the electrochemical *** reconstructed OER catalyst(Fe-doped nickel hydroxide/oxyhydroxide nanosheets supported on Ni foam,Fe-NiO_(x)(OH)y/NF)manifests a low overpotential of 21. mV at 1. mA cm^(-2)and 263 m V at 1.0 m A cm^(-2)in 1.0 M ***,the Fe-NiO_(x)(OH)_(y)/NF also demonstrates high stability in 30 wt.%*** strategy of regulating the electrochemical reconstruction process sheds light on the construction of stable and efficient OER catalysts for industrial water electrolysis.

关键词： oxygen evolution reaction industrial alkaline conditions metal-organic framework complete reconstruction

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