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KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro

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International Journal of Oral Science 2022年第2期14卷 220-230页

作者： Zhuo Chen wenzhi Wu Chen Zheng Yanhua Lan Huizhi xie Zhijian xie Stomatology Hospital School of StomatologyZhejiang University School of MedicineClinical Research Center for Oral Diseases of Zhejiang ProvinceKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceCancer Center of Zhejiang UniversityHangzhouChina

Multiple signaling pathways are involved in the regulation of cell proliferation and differentiation in odontogenesis and dental tissue renewal,but the details of these mechanisms remain ***,we investigated the expression patterns of a transcription factor,Krüppel-like factor 6(KLF6),during the development of murine tooth germ and its function in odontoblastic ***6 was almost ubiquitously expressed in odontoblasts at various stages,and it was co-expressed with P21(to varying degrees)in mouse dental *** determine the function of Klf6,overexpression and knockdown experiments were performed in a mouse dental papilla cell line(iMDP-3).Klf6 functioned as a promoter of odontoblastic differentiation and inhibited the proliferation and cell cycle progression of i MDP-3 through p21 ***-luciferase reporter assay and chromatin immunoprecipitation showed that Klf6 directly activates p21 ***,the in vivo study showed that KLF6 and P21were also co-expressed in odontoblasts around the reparative *** conclusion,Klf6 regulates the transcriptional activity of p21,thus promoting the cell proliferation to odontoblastic differentiation transition in *** study provides a theoretical basis for odontoblast differentiation and the formation of reparative dentine regeneration.

关键词： KLF6 inhibited details

ISO22000《食品安全管理体系要求》在大豆膳食纤维绿茶饮料生产中的应用

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农产品加工（下） 2005年第11期 61-64页

作者：解文志曹龙奎王云华佟玉龙黑龙江八一农垦大学大庆163319 北京贯都咨询公司北京100083

将ISO22000《食品安全管理体系要求》应用于大豆膳食纤维绿茶饮料的生产加工中,对其进行前提方案、产品描述、危害分析和CCP控制等的文件策划及体系运行。

关键词： ISO22000 HACCP 食品安全饮料

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金丝猴颅骨及牙齿的比较形态研究

兽类学报 1983年第2期 131-140页

作者：陈服官闵芝兰罗时有解文治西北大学生物系陕西动物研究所

金丝猴(Rhinopithecus roxellanae M.-E.)自从1870年订名以来,对它的研究多限于分类和个体生态习性的观察描述,至于它的形态解剖方面的论述国内几无报道。国外Hooizer(1950)和Swindler(1976)对其牙齿作了简单扼要的描述,前者并在11例川... 详细信息

金丝猴(Rhinopithecus roxellanae M.-E.)自从1870年订名以来,对它的研究多限于分类和个体生态习性的观察描述,至于它的形态解剖方面的论述国内几无报道。国外Hooizer(1950)和Swindler(1976)对其牙齿作了简单扼要的描述,前者并在11例川金丝猴头骨中发现一例左侧上颌骨具有P~4。这次我们对金丝猴系统的研究过程中,在解剖方面,除了进行各器官系统解剖外,同时还与猕猴、黑叶猴相比较,并对其器官的形态变化和生态生理机能的结合上给以适当注意和解释。

关键词：金丝猴门齿金线猴仰鼻猴属黑叶猴猕猴恒河猴猕猴属颅骨牙齿形态研究

Precipitation amount and frequency affect seedling emergence and growth of Reaumuria soongarica in northwestern China

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Journal of Arid Land 2018年第4期10卷 574-587页

作者： SHAN Lishan ZHAO wenzhi LI Yi ZHANG Zhengzhong xie Tingting Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences College of Forestry Gansu Agricultural University

Climate change is shifting the amount and frequency of precipitation in many regions, which is expected to affect seedling recruitment across ecosystems. However, the combined effects of precipitation amount and frequency on seedling recruitment remain largely unknown. An understanding of the effects of precipitation amount and frequency and their interaction on seedling emergence and growth of typical desert plants is vital for managing populations of desert plants. We conducted two experiments to study the effects of variation in precipitation on Reaumuria soongarica （Pall.） Maxim. First, greenhouse experiments were conducted to examine the effects of three precipitation amount treatments （ambient, ＋30%, and -30%） and two precipitation frequency treatments （ambient and -50%） on seedling emergence. Second, the morphological responses of R. soongarica to changes in precipitation amount and frequency were tested in a controlled field experiment. Stage-specific changes in growth were monitored by sampling in different growth seasons. Our results showed that precipitation amount significantly affected germination, seedling emergence, and growth of R. soongarica, and there was a larger effect with decreased precipitation frequency compared with ambient. Germination and seedling emergence increased as precipitation increased under the same frequency of precipitation. The highest emergence percentage was obtained with a 30% increase in precipitation amount and a 50% reduction in precipitation frequency. Compared with ambient precipitation, a 30% increase in precipitation amount increased above- and below-ground biomass accumulation of R. soongarica during the growth season. A decrease of 30% in precipitation amount also resulted in an increase in below-ground biomass and root/shoot ratio in the early stages of the growth season, however, above- and below-ground biomass showed the opposite results at the end of the growth season, with larger effects on above-ground than be

关键词： precipitation patterns seedling emergence biomass root/shoot ratio seedling recruitment

秦岭地区金丝猴(Rhinopithecus roxellanae)的群体行为与生态习性的观察

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兽类学报 1983年第2期 141-146页

作者：陈服官闵芝兰罗时有解文治西北大学生物系陕西动物研究所

金丝猴(Rhinopithecus roxellanae M.-E)这一我国的珍贵动物,自1870年法国人Milne-Edwards订名以来,直到解放前均只有分类上的描述。解放后,对其生活习性及地理分布(刘诗峰,1959;宋志明、罗志腾,1959;郑永烈,1959;赵肯堂,1959;郑荣梁,19... 详细信息

金丝猴(Rhinopithecus roxellanae M.-E)这一我国的珍贵动物,自1870年法国人Milne-Edwards订名以来,直到解放前均只有分类上的描述。解放后,对其生活习性及地理分布(刘诗峰,1959;宋志明、罗志腾,1959;郑永烈,1959;赵肯堂,1959;郑荣梁,1960)以及种群结构(胡锦矗,1980)等方面均作过不少很有价值的观察报道。本文是在前人工作的基础上,对金丝猴几个行为生态在观察研究的同时,并对其形成这些行为的原因作一初步分析。

关键词：金丝猴群体结构性成熟仔猴金线猴仰鼻猴属群体组成 Rhinopithecus roxellanae 幼猴

Spatiotemporal change patterns of urban lakes in China’s major cities between 1990 and 2015

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International Journal of Digital Earth 2018年第11期11卷 1085-1102页

作者： Cong xie Xin Huang Leiguang Wang Xing Fang wenzhi Liao State Key Laboratory of Information Engineering in Surveying Mapping and Remote Sensing(LIESMARS)Wuhan UniversityWuhanPeople’s Republic of China School of Remote Sensing and Information Engineering Wuhan UniversityWuhanPeople’s Republic of China School of Forestry Southwest Forestry UniversityKunmingPeople’s Republic of China School of Geodesy and Geomatics Wuhan UniversityWuhanPeople’s Republic of China Department of Telecommunications and Information Processing Ghent UniversityGhentBelgium State Key Laboratory of Remote Sensing Science Institute of Remote Sensing and Digital EarthChinese Academy of SciencesBeijingPeople’s Republic of China

China has experienced unprecedented urbanization in the past decades,resulting in dramatic changes in the physical,limnological,and hydrological characteristics of lakes in urban ***,the spatiotemporal dynamics in distribution and abundance of urban lakes in China remain poorly ***,we characterized the spatiotemporal change patterns of urban lakes in China’s major cities between 1990 and 2015 using remote-sensing data and landscape *** results showed that the urban lake landscape patterns have experienced drastic changes over the past 25 *** total surface area of the urban lakes has decreased by 17,620.02 ha,a decrease of 24.22%,with a significant increase in the landscape fragmentation and a reduction in shape *** defined three lake-shrinkage types and found that vanishment was the most common lake-shrinkage pattern,followed by edge-shrinkage and tunneling in terms of lake ***,we also found that urban sprawl was the dominant driver of the lake shrinkage,accounting for 67.89%of the total area loss,and the transition from lakes to cropland was also an important factor(19.86%).This study has potential for providing critical baseline information for government decision-making in lake resources management and urban landscape design.

关键词： Urban expansion lake changes landscape pattern human activities

Differential regulation of H3S10 phosphorylation, mitosis progression and cell fate by Aurora Kinase B and C in mouse preimplantation embryos

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Protein & Cell 2017年第9期8卷 662-674页

作者： wenzhi Li Peizhe Wang Bingjie Zhang Jing Zhang Jia Ming Wei xie Jie Na Center for Stem Cell Biology and Regenerative Medicine School of Medicine Tsinghua University Beijing 100084 China Center for Stem Cell Biology and Regenerative Medicine MOE Key Laboratory of Bioinformatics THU-PKU Center for LifeSciences School of Life Sciences Tsinghua University Beijing 100084 China

Coordination of cell division and cell fate is crucial for the successful development of mammalian early embryos. Aurora kinases are evolutionarily conserved serine/threonine kinases and key regulators of mitosis. Aurora kinase B （AurkB） is ubiquitously expressed while Aurora kinase C （AurkC） is specifically expressed in gametes and preimplantation embryos. We found that increasing AurkC level in one blastomere of the 2-cell embryo accelerated cell division and decreasing AurkC level slowed down mitosis. Changing AurkB level had the opposite effect. The kinase domains of AurkB and AurkC were responsible for their different ability to phosphorylate Histone H3 Serine 10 （H3S10P） and regulate metaphase timing. Using an Oct4-photoactivat- able GFP fusion protein （Oct4-paGFP） and fluorescence decay after photoactivation assay, we found that AurkB overexpression reduced Oct4 retention in the nucleus. Finally, we show that blastomeres with higher AurkC level elevated pluripotency gene expression, which were inclined to enter the inner cell mass lineage and subsequently contributed to the embryo proper. Collectively, our results are the first demonstration that the activity of mitotic kinases can influence cell fate decisions in mammalian preimplantation embryos and have important implications to assisted reproduction.

关键词： Aurora kinase mouse preimplantation embryo cell fate development mitosis

Polydopamine nanoparticle-mediated mild photothermal therapy for inhibiting atherosclerotic plaque progression by regulating lipid metabolism of foam cells

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Regenerative Biomaterials 2023年第1期10卷 830-838页

作者： Shuangshuang Tu wenzhi Ren Jinru Han Haijing Cui Ting Dai Haoxuan Lu Yanqing xie Wenming He Aiguo Wu Department of Cardiology The First Affiliated Hospital of Ningbo UniversityNingboZhejiang Province 315020China Cixi Institute of Biomedical Engineering International Cooperation Base of Biomedical Materials Technology and ApplicationChinese Academy of Science(CAS)Key Laboratory of Magnetic Materials and Devices&Zhejiang Engineering Research Center for Biomedical MaterialsNingbo Institute of Materials Technology and Engineering CASNingbo 315201China Advanced Energy Science and Technology Guangdong Laboratory Huizhou 516000China University of Chinese Academy of Sciences Beijing 101408China

Since apoptosis of foam,cells can induce plaque instability,reducing intracellular lipid content while protecting foam cells from apoptosis is beneficial for the safe and efficient therapy of *** this study,osteopontin-coupled polydopamine(PDA-OPN)nanoparticles were synthesized and applied to target mild photothermal therapy(PTT)of *** results from laser confocal microscopy indicate that PDA-OPN nanoparticles can be specially recognized and absorbed by foam *** near-infrared laser irradiation,the mild photothermal generated by PDA-OPN decreases intracellular lipid accumulation but does not induce cell *** vivo treatments demonstrate that mild PTT can substantially reduce plaque area and improve plaque stability by upregulating the expression of plaque fibrosis in ApoE^(−/−)*** findings reinforce that the PDA-OPN nanoparticle-mediated mild PTT can inhibit atherosclerotic progression,which provides new insights for developing safe and effective treatment methods for atherosclerosis.

关键词： polydopamine nanoparticles photothermal therapy atherosclerosis lipid metabolism

The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (Linum usitatissimum L.)

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Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei xie Jing Li Wan Li Lijun Sun Zhigang Dai wenzhi Zhou Jianguang Su Jian Sun School of Life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu Biotechnology Co.Ltd. Nantong 226001China

Flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction