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Prepartum body conditions affect insulin signaling pathways in postpartum adipose tissues in transition dairy cows

Journal of Animal Science and Biotechnology 2019年第3期10卷 648-657页

作者： Fanjian Zhang Dan Li Qiong Wu Jian Sun wenyi guan Yinxu Hou Yaohong Zhu Jiufeng Wang Beijing Vocational College of Agriculture Beijing 102442People’s Republic of China College of Veterinary Medicine China Agricultural UniversityBeijing 100193People’s Republic of China Animal Science and Technology College Beijing University of AgricultureBeijing 102206People’s Republic of China

Background: Overconditioned dairy cows are susceptible to excessive lipolysis and increased insulin resistance during the transition *** associations among body fat reserve,insulin resistance,and lipolysis in adipose tissues(AT) remain to be ***,this study aimed to investigate whether excessive fat reserves influence the insulin signaling pathway in AT ***: Twenty multiparous dairy cows were selected and assigned to one of two groups,according to prepartum body condition score(BCS): Control group(BCS = 3.0–3.5;n = 10) and Overconditioned group(BCS ≥ 4.0;n = 10).Blood samples were collected on days-14,-7,-4,-2,-1,0,1,2,4,7,and 14 relative to *** AT were collected on day 2 following parturition for quantitative real-time polymerase chain reaction and western blot *** differences were observed between the two groups in serum glucose,non-esterified fatty acids,β-hydroxybutyric acid,tumor necrosis factor(TNF)α,insulin,or leptin concentrations during the experimental *** with the control cows,the overconditioned cows had lower serum triglyceride levels and higher adiponectin *** the AT postpartum,insulin receptor mRNA and protein levels were lower in the overconditioned cows than in the control cows,and no differences were found in glucose transporter 4 *** with the control cows,the overconditioned cows had lower mRNA levels of TNFα and higher mRNA levels of peroxisome proliferator-activated receptor gamma(PPARγ) in AT *** phosphorylated protein kinase B(AKT) content and phosphorylation rate of AKT were increased in the overconditioned cows compared with the control cows,which suggested that the downstream insulin signaling in AT was ***: In the present study,transition dairy cows with higher BCS did not show more fat *** changes of insulin signaling pathway in AT postpartum of overconditioned cows may be partly related to the ex

关键词： Adipose tissue Body condition score Insulin signaling pathway Transition dairy cow

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COVID-19: Antiviral Agents, Antibody Development and Traditional Chinese Medicine

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Virologica Sinica 2020年第6期35卷 685-698页

作者： wenyi guan Wendong Lan Jing Zhang Shan Zhao Junxian Ou Xiaowei Wu Yuqian Yan Jianguo Wu Qiwei Zhang guangdong Provincial Key Laboratory of Tropical Disease Research School of Public HealthSouthern Medical UniversityGuangzhou 510515China guangdong Provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou 510632GuangdongChina

The World Health Organization(WHO) has declared coronavirus disease 2019(COVID-19) is the first pandemic caused by coronavirus named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). Currently, there is no effective anti-SARS-CoV-2 drug approved worldwide for treatment of patients with COVID-19. Therapeutic options in response to the COVID-19 outbreak are urgently needed. To facilitate the better and faster development of therapeutic COVID-19 drugs, we present an overview of the global promising therapeutic drugs, including repurposing existing antiviral agents,network-based pharmacology research, antibody development and traditional Chinese medicine. Among all these drugs,we focus on the most promising drugs(such as favipiravir, tocilizumab, SARS-CoV-2 convalescent plasma, hydroxychloroquine, Lianhua Qingwen, interferon beta-1 a, remdesivir, etc.) that have or will enter the final stage of human testing—phase Ⅲ–Ⅳ clinical trials.

关键词： Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) Coronavirus disease 2019(COVID-19) Updates Drug repositioning Network-based pharmacology Antibody Traditional Chinese medicine

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Construction and Characterization of a Novel Recombinant Attenuated and Replication-Deficient Candidate Human Adenovirus Type 3 Vaccine:"Adenovirus Vaccine Within an Adenovirus Vector"

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Virologica Sinica 2021年第3期36卷 354-364页

作者： Yuqian Yan Shuping Jing Liqiang Feng Jing Zhang Zhiwei Zeng Min Li Shan Zhao Junxian Ou Wendong Lan wenyi guan Xiaowei Wu Jianguo Wu Donald Seto Qiwei Zhang guangdong Provincial Key Laboratory of Tropical Disease Research School of Public HealthSouthern Medical UniversityGuangzhou 510515China Microbiological Laboratory Zhuhai Center for Disease Control and PreventionZhuhai 519000China guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China guangdong Provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou 510632China Bioinformatics and Computational Biology Program School of Systems BiologyGeorge Mason UniversityManassasVA 20110USA

Human adenoviruses(HAd Vs)are highly contagious and result in large number of acute respiratory disease(ARD)cases with severe morbidity and *** adenovirus type 3(HAd V-3)is the most common type that causes ARD outbreaks in Asia,Europe,and the ***,there is currently no vaccine approved for its general *** hexon protein contains the main neutralizing epitopes,provoking strong and lasting *** this study,a novel recombinant and attenuated adenovirus vaccine candidate against HAd V-3 was constructed based on a commercially-available replication-defective HAd V-5 gene therapy and vaccine *** entire HAd V-3 hexon gene was integrated into the E1 region of the vector by homologous recombination using a bacterial *** resultant recombinants expressing the HAd V-3 hexon protein were rescued in AD293 cells,identified and characterized by RT-PCR,Western blots,indirect immunofluorescence,and electron *** potential vaccine candidate had a similar replicative efficacy as the wild-type HAd V-3 ***,and importantly,the vaccine strain had been rendered replication-defective and was incapable of replication in A549 cells after more than twentygeneration passages in AD293 *** represents a significant safety *** mice immunized both intranasally and intramuscularly by this vaccine candidate raised significant neutralizing antibodies against HAd ***,this recombinant,attenuated,and safe adenovirus vaccine is a promising HAd V-3 vaccine *** strategy of using a clinically approved and replication-defective HAd V-5 vector provides a novel approach to develop universal adenovirus vaccine candidates against all the other types of adenoviruses causing ARDs and perhaps other adenovirus-associated diseases.

关键词： Adenovirus vaccine Human adenovirus type 3(HAdV-3) Replication-deficient adenovirus vector Immunity in BALB/c mice Recombination

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Desmoglein 2(DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia

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Virologica Sinica 2021年第6期36卷 1400-1410页

作者： Jing Zhang Kui Ma Xiangyu Wang Yinbo Jiang Shan Zhao Junxian Ou Wendong Lan wenyi guan Xiaowei Wu Heping Zheng Bin Yang Chengsong Wan Wei Zhao Jianguo Wu Qiwei Zhang guangdong Provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou510632China guangdong Provincial Key Laboratory of Tropical Disease Research School of Public HealthSouthern Medical UniversityGuangzhou510515China guangdong Provincial Dermatology Hospital Southern Medical UniversityGuangzhou510091China

Human adenovirus type 55(HAdV-B55) is a re-emergent acute respiratory disease pathogen that causes adult communityacquired pneumonia(CAP). Previous studies have shown that the receptor of HAdV-B14, which genome is highly similar with HAdV-B55, is human Desmoglein 2(DSG2). However, whether the receptor of HAdV-B55 is DSG2 is undetermined because there are three amino acid mutations in the fiber gene between HAdV-B14 and HAdV-B55. Here, firstly we found the 3T3 cells, a mouse embryo fibroblast rodent cell line which does not express human DSG2, were able to be infected by HAdV-B55 after transfected with pcDNA3.1-DSG2, while normal 3T3 cells were still unsusceptible to HAdV-B55 infection. Next, A549 cells with h DSG2 knock-down by siRNA were hard to be infected by HAdV-B3/-B14/-B55, while the control siRNA group was still able to be infected by all these types of HAdVs. Finally, immunofluorescence confocal microscopy indicated visually that Cy3-conjugated HAdV-B55 viruses entered A549 cells by binding to DSG2 ***, DSG2 is a major receptor of HAdV-B55 causing adult CAP. Our finding is important for better understanding of interactions between adenoviruses and host cells and may shed light on the development of new drugs that can interfere with these processes as well as for the development of potent prophylactic vaccines.

关键词： Human adenovirus type 55(HAdV-B55) Severe community-acquired pneumonia Adenovirus receptor Desmoglein 2(DSG2)

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参照动物福利国际标准,规范兽医临床实践教学

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畜牧与兽医 2019年第3期51卷 126-128页

作者：关文怡张凡建蔡泽川北京农业职业学院北京房山102442

兽医临床实验实训教学在兽医临床课程体系中占有重要地位,而实验动物在教学中起到重要的支撑作用,要在保证实验实训教学质量和学生实践能力培养的基础上,同时兼顾实验动物福利。本文阐述了兽医临床实验实训教学的特点、动物福利的内涵... 详细信息

兽医临床实验实训教学在兽医临床课程体系中占有重要地位,而实验动物在教学中起到重要的支撑作用,要在保证实验实训教学质量和学生实践能力培养的基础上,同时兼顾实验动物福利。本文阐述了兽医临床实验实训教学的特点、动物福利的内涵与应用意义及动物福利在教学中的应用,为能在保证教学要求的同时更合理使用实验动物提供参考。

关键词：动物福利兽医临床实验实训实验动物

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葡萄籽粕替代玉米对种鸡产蛋高峰后期生产性能、鸡蛋品质及新城疫抗体水平的影响

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中国畜牧兽医 2019年第12期46卷 3538-3546页

作者：乔利敏关文怡马建民北京农业职业学院

试验旨在研究葡萄籽粕对种鸡产蛋高峰后期生产性能、鸡蛋品质及新城疫抗体水平的影响。选取产蛋率、体重相近,健康状态良好的64周龄罗曼灰父母代种鸡810只,随机分为6组,每组5个重复,每个重复27只鸡。对照组饲喂基础日粮,试验组分别饲喂... 详细信息

试验旨在研究葡萄籽粕对种鸡产蛋高峰后期生产性能、鸡蛋品质及新城疫抗体水平的影响。选取产蛋率、体重相近,健康状态良好的64周龄罗曼灰父母代种鸡810只,随机分为6组,每组5个重复,每个重复27只鸡。对照组饲喂基础日粮,试验组分别饲喂葡萄籽粕替代基础日粮中3%、4.5%、6%、7.5%、9%玉米的试验日粮(分别为试验Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ组)。预试期7 d,正试期30 d。探讨日粮中添加不同水平的葡萄籽粕对种鸡产蛋高峰后期生产性能、鸡蛋品质及新城疫抗体水平的影响及其作用机理。结果表明:葡萄籽粕能够明显抑制种鸡产蛋高峰后期生产性能降低,对于日均产蛋量,试验Ⅲ组极显著高于对照组(P0.05);葡萄籽粕处理组鸡蛋的哈氏单位、蛋黄重及蛋壳强度均高于对照组,但随葡萄籽粕添加量的增加没有表现出规律性变化;葡萄籽粕能够降低鸡蛋胆固醇含量(P0.05)。综上,在种鸡产蛋高峰后期日粮中以葡萄籽粕替代玉米,可提高种鸡生产性能和新城疫抗体效价,改善鸡蛋品质,降低鸡蛋中胆固醇含量,且以替代6%的玉米效果较好。

关键词：葡萄籽粕种鸡生产性能鸡蛋品质新城疫抗体水平

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添加秸秆堆肥处理厨余垃圾过程中蛋白类物质转化及微生物群落研究

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华中科技大学学报（自然科学版） 2022年第10期50卷 83-96页

作者：欧蓓薛映肖可可杨家宽陈海滨关文义程谦勋华中科技大学环境科学与工程学院湖北武汉430074 安徽国祯环卫科技有限公司安徽合肥230000

将玉米、水稻、油菜和小麦四种农业秸秆作为填充剂与厨余垃圾进行堆肥,分析堆体理化性质,利用不同表征方式分析堆体成熟度、稳定度变化情况,并采用高通量测序技术研究堆肥不同阶段的微生物菌群特性.结果表明:依照堆肥成熟要求,经35 d堆... 详细信息

将玉米、水稻、油菜和小麦四种农业秸秆作为填充剂与厨余垃圾进行堆肥,分析堆体理化性质,利用不同表征方式分析堆体成熟度、稳定度变化情况,并采用高通量测序技术研究堆肥不同阶段的微生物菌群特性.结果表明:依照堆肥成熟要求,经35 d堆肥后,仅未添加填充剂的堆肥处理组不满足堆肥成熟要求,而四组不同填充剂的堆肥产品均满足堆肥成熟要求;添加填充剂可以促进降解纤维素的变形菌门和拟杆菌门的富集;当堆肥过程结束时,4个秸秆组存在纤维弧菌和糖单孢菌等具有分解纤维素等难溶性有机质能力的菌属;与堆肥初期相比,添加玉米秸秆后,腐熟期氨基酸代谢序列的相对丰度增加了8.9%,且脯氨酸含量增加量最高,腐熟期时增加至129.69 mmol/kg.相较而言,玉米秸秆是较优的填充剂.

关键词：厨余垃圾好氧堆肥秸秆填充剂蛋白质氨基酸微生物群落

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丁酸梭菌对断奶仔猪生长性能及肠道菌群结构的影响

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黑龙江畜牧兽医 2019年第12期 103-105页

作者：孙健乔立东雷莉辉关文怡北京农业职业学院畜牧兽医系

为了进一步研究丁酸梭菌对断奶仔猪生长性能及肠道菌群结构的影响,试验选择健康的杜长大三元杂交断奶仔猪72头,随机分为2组,对照组饲喂基础日粮,试验组在基础日粮中添加丁酸梭菌1×108cfu/kg,每组6个重复,每个重复6头猪,试验期为28 d,... 详细信息

为了进一步研究丁酸梭菌对断奶仔猪生长性能及肠道菌群结构的影响,试验选择健康的杜长大三元杂交断奶仔猪72头,随机分为2组,对照组饲喂基础日粮,试验组在基础日粮中添加丁酸梭菌1×108cfu/kg,每组6个重复,每个重复6头猪,试验期为28 d,观察日粮中添加丁酸梭菌对仔猪日采食量、日增重、料重比、腹泻率等指标的影响,并于断奶后28天采集新鲜粪便进行16S rRNA微生物组学分析。结果表明:丁酸梭菌可显著降低料重比(P0. 05)。说明丁酸梭菌可有效改善仔猪肠道菌群结构,从而提高饲料转化效率。

关键词：丁酸梭菌肠道断奶仔猪生长性能菌群 16S rRNA

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奶牛热应激及其营养调控研究进展

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中国畜牧杂志 2021年第6期57卷 73-77页

作者：张凡建关文怡蔡泽川孙健侯引绪北京农业职业学院畜牧兽医系北京102442

随着全球性气候变暖,奶牛生产规模化程度不断提高、高产奶牛不断选育,热应激已成为全球乳制品行业面临的重要挑战。热应激程度与外界环境因素和奶牛自身因素有关。夏季热应激会导致奶牛产奶量降低、乳成分改变和繁殖性能下降,还会给奶... 详细信息

随着全球性气候变暖,奶牛生产规模化程度不断提高、高产奶牛不断选育,热应激已成为全球乳制品行业面临的重要挑战。热应激程度与外界环境因素和奶牛自身因素有关。夏季热应激会导致奶牛产奶量降低、乳成分改变和繁殖性能下降,还会给奶牛健康、免疫和福利带来负面影响。营养调控措施在缓解奶牛热应激方面起到重要作用。本文概述了奶牛热应激的产生及其对生产、繁殖、健康与福利的影响,总结了通过营养调控策略缓解奶牛热应激的研究进展,展望了奶牛热应激的未来研究方向。

关键词：热应激奶牛生产健康营养调控

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安徽省美好乡村生活垃圾分类处理模式研究

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环境与发展 2019年第4期31卷 31-32页

作者：关文义王宁汪小会安徽国祯环卫科技有限公司

通过选取安徽省凤阳县小岗村为垃圾分类试点,对试点生活垃圾产生量、物理组分、处理情况等的现状调研,建立了安徽省美好乡村生活垃圾分类模式和分类收运处理体系,并对美好乡村生活垃圾分类试点工作的长期稳定开展提出建议。

关键词：美好乡村生活垃圾生活垃圾分类分类模式

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