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Human Placental Mesenchymal Stem Cells Relieve Primary Sclerosing Cholangitis via Upregulation of TGR5 in Mdr2-/-Mice and Human Intrahepatic Cholangiocyte Organoid Models

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Research 2024年第2期2023卷 355-372页

作者： Qigu Yao wenyi chen Yingduo Yu Feiqiong Gao Jiahang Zhou Jian Wu Qiaoling Pan Jinfeng Yang Lingling Zhou Jiong Yu Hongcui Cao Lanjuan Li State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases Cllaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affliated HospitalZhejang University School of Medicine79 Qingchun Rd.Hangzhou 310003China National Clinical Research Center for Infectious Diseases HangzhouChina Key Laboratory of Diagnosis and Treatment of Aging and Physic-chemical Injury Diseases of Zhejiang Province 79 Qingchun Rd.Hangzhou 310003China Jjinan Microecological Biomedicine Shandong Laboratory Jinan 250117China

Primary sclerosing cholangitis(PSC)is a biliary disease accompanied by chronic inflammation of the liver and biliary *** stem cells(MSCs)are used to treat liver diseases because of their immune regulation and regeneration-promoting *** study was performed to explore the therapeutic potential of human placental MSCs(hP-MSCs)in PSC through the Takeda G protein-coupled receptor 5(TGR5)receptor *** tissues were collected from patients with PSC and healthy donors(n=4)for RNA sequencing and intrahepatic cholangiocyte organoid ***-MSCs were injected via the tail vein into Mdr2^(-/-),bile duct ligation(BDL),and 3,5-diethoxycarbonyl-1,4-dihydrocollidine(DDC)mouse models or co-cultured with organoids to confirm their therapeutic effect on biliary *** in bile acid metabolic profile were analyzed by liquid chromatography/tandem mass spectrometry(LC-MS/MS).Compared with healthy controls,liver tissues and intrahepatic cholangiocyte organoids from PSC patients were characterized by inflammation and cholestasis,and marked downregulation of bile acid receptor TGR5 ***-MSC treatment apparently reduced the inflammation,cholestasis,and fibrosis in Mdr2^(-/-),BDL,and DDC model *** activating the phosphatidylinositol 3 kinase/extracellular signal-regulated protein kinase pathway,hP-MSC treatment promoted the proliferation of cholangiocytes,and affected the transcription of downstream nuclear factorκB through regulation of the binding of TGR5 and Pellino3,thereby affecting the cholangiocyte inflammatory phenotype.

关键词： inflammation TGR5 Human

MSC-derived exosomes attenuate hepatic fibrosis in primary sclerosing cholangitis through inhibition of Th17 differentiation

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Asian Journal of Pharmaceutical Sciences 2024年第1期19卷 119-134页

作者： wenyi chen Feiyan Lin Xudong Feng Qigu Yao Yingduo Yu Feiqiong Gao Jiahang Zhou Qiaoling Pan Jian Wu Jinfeng Yang Jiong Yu Hongcui Cao Lanjuan Li State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Medical Center for Infectious DiseasesThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China Jinan Microecological Biomedicine Shandong Laboratory Jinan 250117China Key Laboratory of Diagnosis and Treatment of Aging and Physic-chemical Injury Diseases of Zhejiang Province Hangzhou 310003China

Primary sclerosing cholangitis(PSC)is an autoimmune cholangiopathy characterized by chronic inflammation of the biliary epithelium and periductal fibrosis,with no curative treatment available,and liver transplantation is inevitable for end-stage *** placentalmesenchymal stem cell(hpMSC)-derived exosomes have demonstrated the ability to prevent fibrosis,inhibit collagen production and possess immunomodulatory properties in autoimmune liver ***,we prepared hpMSC-derived exosomes(Exo^(MSC))and further investigated the anti-fibrotic effects and detailed mechanism on PSC based on Mdr2^(−/−)mice and multicellular organoids established from PSC *** results showed that Exo^(MSC) ameliorated liver fibrosis in Mdr2^(−/−)mice with significant collagen reduction in the preductal area where Th17 differentiation was inhibited as demonstrated by RNAseq analysis,and the percentage of CD4+IL-17A+T cells was reduced both in Exo^(MSC)-treated Mdr2^(−/−)mice(Mdr2^(−/−)-Exo)in vivo and Exo^(MSC)-treated Th17 differentiation progressed in ***,Exo^(MSC) improved the hypersecretory phenotype and intercellular interactions in the hepatic Th17 microenvironment by regulating PERK/CHOP signaling as supported by multicellular ***,our data demonstrate the antifibrosis effect of Exo^(MSC) in PSC disease by inhibiting Th17 differentiation,and ameliorating the Th17-induced microenvironment,indicating the promising potential therapeutic role of Exo^(MSC) in liver fibrosis of PSC or Th17-related diseases.

关键词： Mesenchymal stem cell Exosomes Primary sclerosing cholangitis Fibrosis Organoids Th17

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水稻糊粉层蛋白酶体参与种子萌发的研究

热带作物学报 2024年第4期45卷 752-760页

作者：陈文奕陈惠萍海南大学生命科学学院海南海口570228

种子萌发是植物进入营养生长阶段的关键性环节,在水稻(Oryza sativa L.)种子萌发过程中,糊粉层会合成并分泌一系列的酸性水解酶(如α-淀粉酶、蛋白酶)用于降解胚乳中的贮藏物质,为水稻幼苗生长提供营养。水稻种子贮藏蛋白的降解离不开... 详细信息

种子萌发是植物进入营养生长阶段的关键性环节,在水稻(Oryza sativa L.)种子萌发过程中,糊粉层会合成并分泌一系列的酸性水解酶(如α-淀粉酶、蛋白酶)用于降解胚乳中的贮藏物质,为水稻幼苗生长提供营养。水稻种子贮藏蛋白的降解离不开蛋白酶体及蛋白酶的作用。为探究26S蛋白酶体、20S蛋白酶体及其亚基PBA1参与GA对萌发水稻种子糊粉层中贮藏蛋白的调节过程,本研究以水稻杂交种特优128为试验材料,通过采用酶联免疫吸附技术、实时荧光定量PCR技术等方法,检测26S蛋白酶体、20S蛋白酶体及PBA1的活性和OsPBA1转录水平,以及总蛋白含量的变化。结果表明:GA明显促进糊粉层细胞中贮藏蛋白的降解,加快水稻种子的萌发;而在GA合成受阻时,糊粉层细胞中贮藏蛋白的降解进程延缓,从而延迟水稻种子的萌发。正常培养条件下,水稻种子糊粉层中26S蛋白酶体活性和20S蛋白酶体活性的峰值分别出现在6 d和7 d,而OsPBA1表达水平及PBA1活性则在5 d和8 d均呈现峰值。进一步研究表明,GA可以诱导PBA1、20S蛋白酶体及26S蛋白酶体的活性,从而加快种子萌发的进程;而在26S蛋白酶体活性抑制剂MG132处理下,抑制了萌发水稻种子的生长,但提高了20S蛋白酶体和PBA1的活性。以上研究初步揭示了26S蛋白酶体在GA调节水稻种子糊粉层贮藏蛋白降解中的作用及其对种子萌发的影响。

关键词： GA 蛋白酶体水稻糊粉层贮藏蛋白种子萌发

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论侯孝贤电影悲剧美学的创作手法

盐城工学院学报（社会科学版） 2024年第2期37卷 60-65页

作者：陈文奕集美大学海洋文化与法律学院福建厦门361021

侯孝贤电影的底色是“苍凉”,其电影主题彰显出浓重的悲剧意识,体现了中华文化“哀而不伤”的诗教传统。侯孝贤电影的悲剧美学手法不仅是对传统电影语言的创新,也是其传达悲剧精神的有力载体。探究了侯孝贤电影的悲剧镜头语言、悲剧抒... 详细信息

侯孝贤电影的底色是“苍凉”,其电影主题彰显出浓重的悲剧意识,体现了中华文化“哀而不伤”的诗教传统。侯孝贤电影的悲剧美学手法不仅是对传统电影语言的创新,也是其传达悲剧精神的有力载体。探究了侯孝贤电影的悲剧镜头语言、悲剧抒情方式和悲剧意境,凸显侯孝贤电影作为一种悲剧审美形态的价值,不仅是对美与真实的坚守,更是对生命伦理的一种传承。

关键词：侯孝贤电影悲剧美学悲剧意识

基于DeepLabv3+算法的城市街景语义分割算法研究

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无线互联科技 2024年第9期21卷 103-105页

作者：陈文艺苗宗成西京学院电子信息学院陕西西安710123

在自动驾驶领域,城市街道场景的语义分割对于提升系统的安全性和效率至关重要。针对传统语义分割模型参数过多、泛化性能较差以及分割效果有限等问题,文章提出了一种基于改进DeepLabv3+的解决方案。此改进模型融合了轻量级MobileNetv2... 详细信息

在自动驾驶领域,城市街道场景的语义分割对于提升系统的安全性和效率至关重要。针对传统语义分割模型参数过多、泛化性能较差以及分割效果有限等问题,文章提出了一种基于改进DeepLabv3+的解决方案。此改进模型融合了轻量级MobileNetv2主干网络和SE注意力机制,优化了空洞金字塔池化(Atrous Spatial Pyramid Pooling,ASPP)模块,将其从并行结构改进成串行结构,采用深度可分离卷积结构。在Cityscapes数据集上,文章的方法取得了75.90%的平均交并比(Mean Intersection over Union,MIoU),显著提升了分割精度与计算效率。

关键词：深度学习语义分割 ASPP

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算法时代我国主流意识形态安全研究评述

佛山科学技术学院学报（社会科学版） 2024年第1期42卷 5-13页

作者：陈文祎岳爱武江苏省习近平新时代中国特色社会主义思想研究中心南京信息工程大学基地江苏南京210044 上海大学马克思主义学院上海宝山200444

伴随信息化技术的迅猛发展以及大数据的全面普及应用,原本只存在于人工智能领域的算法被推上“前台”,凭借其技术逻辑与技术特性,逐渐僭越其技术中立性,日益同各类意识形态共生共融共进。学界对算法时代我国主流意识形态安全的研究聚焦... 详细信息

伴随信息化技术的迅猛发展以及大数据的全面普及应用,原本只存在于人工智能领域的算法被推上“前台”,凭借其技术逻辑与技术特性,逐渐僭越其技术中立性,日益同各类意识形态共生共融共进。学界对算法时代我国主流意识形态安全的研究聚焦在如下层面,即我国主流意识形态安全面临资本逻辑渗透、“受众本位”重塑、“茧房效应”遮蔽、算法“黑箱”操弄等挑战,呈现出方法隐蔽、手段多样、传播面广、破坏性强等新特点,对此应从价值、技术、平台、主体与法治等维度进行有效防治。就研究现状而论,学界深化了研究层次、创新了研究视角、丰富了研究内容与方式,但也存在研究前瞻性欠缺、研究针对性薄弱、研究内容重叠、基础理论研究逊色等不足。今后,我们需要注重经典文本、增强问题意识、补齐理论短板、加强观点创新、增强前瞻意识以及拓展研究思路,为不断深化研究做出不懈努力。

关键词：算法主流意识形态意识形态安全

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大学生共同富裕政策认同量表的设计与检验

宁波教育学院学报 2024年第3期26卷 74-78页

作者：陈文祎王智腾宁波大学外国语学院浙江宁波315211 宁波大学机械工程与力学学院浙江宁波315211

大学生对共同富裕的政策认同情况直接影响着他们参与共同富裕实践的行为。基于政策认同理论构建大学生共同富裕政策认同概念,编制“大学生共同富裕政策认同量表”。通过分析浙江省26所高校的调查数据发现:问卷开发过程严谨,信效度良好;... 详细信息

大学生对共同富裕的政策认同情况直接影响着他们参与共同富裕实践的行为。基于政策认同理论构建大学生共同富裕政策认同概念,编制“大学生共同富裕政策认同量表”。通过分析浙江省26所高校的调查数据发现:问卷开发过程严谨,信效度良好;“四因子模型”具有良好的信效度;对后续开展大学生共同富裕政策认同研究具有启发意义。

关键词：大学生共同富裕政策认同量表开发

Complex coacervation microcapsules by tannic acid crosslinking prolong the antifungal activity of cinnamaldehyde against Aspergillus brasiliensis

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Food Bioscience 2022年第3期47卷 776-784页

作者： wenyi chen Shuqin Xia Chaogeng Xiao Institute of Food Science Zhejiang Academy of Agricultural SciencesHangzhou310021China State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China School of Food Science and Technology Collaborative Innovation Center of Food Safety and Quality ControlJiangnan University1800 Lihu RoadWuxiJiangsu214122China

The antifungal activity and encapsulation properties of cinnamaldehyde-loaded microcapsules by tannic acid were *** results showed that cinnamaldehyde exhibited a high in vitro antifungal effect against Aspergillus brasiliensis through altering the morphology of the hyphae,and its inhibition zone diameter(IZD)exceeded 15 mm with a minimum inhibitory concentration of 1.0 mg/***-loaded microcapsules crosslinked with tannic acid have a high encapsulation efficiency(92.53±5.20%)and antifungal activity(37.82±0.63 mm),and the retention rate was 2.5 times of that of unencapsulated cinnamaldehyde after 30-d storage at 37℃.Furthermore,the antifungal stability was enhanced(IZD 25.37 mm)after 60-d storage at 37℃ as compared with that of cinnamaldehyde(IZD 22.07 mm).The results of cell membrane integrity and the extracellular protein content also indicated that the microencapsulation could effectively protect cinnamaldehyde,delay the decrease of antifungal effect and improve its long-term antifungal activity against *** by increasing the retention rate of cinnamaldehyde after storage.

关键词： Cinnamaldehyde Microcapsules Tannic acid Antifungal stability Antifungal mechanisms

Experimental detection of bubble-wall interactions in a vertical gas–liquid flow

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Chinese Journal of Chemical Engineering 2017年第7期25卷 838-847页

作者： Xing Wang Jiao Sun Jie Zhao wenyi chen Department of Process Equipment and Control Engineering Hehei University oI TechnologyTianjin 300130China Research Center of Engineering Fluid and Process Enhancement Hehei University of TechnologyTianiin 300130China Department of Mechanics School of Mechanical Engineering Tianjin UniversityTianjin 300350China State Key Laboratory of Coal-based Low Carbon Energy ENN Sdence&Development Co.Ltd.Langfang 065001China

Bubble motions and bubble-wall interactions in stagnant liquid were experimentally investigated by high-speed CCD and PIV technique with the main feature parameters such as E?tv?s numbers Eo = 0.98–1.10, Morton number Mo = 3.21 × 10^(-9)and Reynolds numbers Re = 180 ~ 190. The effect of bubble injecting frequency and the distance S between the gas injection nozzle and the wall on the statistical trajectory of bubbles, average velocity distribution of flow field and Reynolds shear stress were studied in detail. It was shown that the combination of bubble injecting frequency and the distance S caused different bubble motion forms and hydrodynamic *** the normalized initial distance was very little, like S*≈ 1.2(here S*= 2S/d_e, and deis the bubble equivalent diameter), bubbles ascended in a zigzag trajectory with alternant structure of high and low speed flow field around the bubbles, and the distribution of positive and negative Reynolds shear stress looked like a blob. With the increase of distance S*, bubbles' trajectory would tend to be smooth and straight from the zigzag curve. Meanwhile, with the increase of bubble injecting frequency, the camber of bubble trajectory at 20

关键词：气泡运动相互作用垂直面气液两相流实验雷诺兹数剪切应力流场结构