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基于伪无菌实验与肠菌移植技术研究气滞证慢传输型便秘模型大鼠肠道菌群对肠道动力的影响

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中医杂志 2024年第9期65卷 943-948页

作者：刘启鸿柯晓骆云丰胡露楠任彦方文怡赵培琳严锦贤福建中医药大学附属第二人民医院福建省福州市350003 福建省医疗机构中药制剂重点实验室

目的运用伪无菌实验与肠菌移植技术,探讨气滞证慢传输型便秘(STC)大鼠的肠道菌群对肠道动力的影响以及二者的关系。方法24只Wistar大鼠随机分为正常组6只、气滞证STC组6只、伪无菌组12只。气滞证STC组采用洛哌丁胺混悬液3 mg/kg灌胃联... 详细信息

目的运用伪无菌实验与肠菌移植技术,探讨气滞证慢传输型便秘(STC)大鼠的肠道菌群对肠道动力的影响以及二者的关系。方法24只Wistar大鼠随机分为正常组6只、气滞证STC组6只、伪无菌组12只。气滞证STC组采用洛哌丁胺混悬液3 mg/kg灌胃联合夹尾刺激建立气滞证STC大鼠模型,造模成功后分别采集气滞证STC组和正常组大鼠的新鲜粪便制备100 mg/ml粪菌混悬液。伪无菌组采用抗生素鸡尾酒法(给予含杆菌肽、硫酸链霉素、硫酸新霉素各20 mg/ml的混合抗生素混悬液灌胃)建立伪无菌大鼠模型,造模成功后将大鼠随机分为正常粪菌液组、气滞证STC粪菌液组各6只,再分别给予制备的相应大鼠粪菌混悬液10 ml/kg灌胃;气滞证STC组大鼠给予等体积无菌水灌胃,均每日1次、连续7天。比较气滞证STC组、正常粪菌液组、气滞证STC粪菌液组小肠推进率;ELISA法检测血清5-羟色胺(5-HT)水平;免疫组化法检测结肠组织5-羟色胺3受体(5-HT3R)、5-羟色胺4受体(5-HT4R)阳性表达水平;Western blot法检测结肠组织色氨酸羟化酶1(TPH1)、色氨酸羟化酶2(TPH2)、5-羟色胺转运体(SERT)、单胺氧化酶A(MAO-A)蛋白表达水平。结果与正常粪菌液组比较,气滞证STC组、气滞证STC粪菌液组大鼠小肠推进率、血清5-HT水平,结肠组织5-HT3R、5-HT4R阳性表达以及TPH1、TPH2、SERT、MAO-A蛋白表达均降低(P0.05)。结论气滞证STC大鼠的肠道菌群紊乱可导致肠道传输功能减慢,其机制可能是通过影响5-HT的合成、转运、代谢等途径而导致肠道动力障碍。

关键词：慢传输型便秘气滞证伪无菌实验肠菌移植肠道菌群肠道动力

不同形态氮素对春小麦苗期生理特性及氮代谢酶的影响

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作物杂志 2011年第1期 20-24页

作者：刘东军张宏纪祁倩倩孙岩郭怡梵孙光祖王广金闫文义杨淑萍马淑梅黑龙江省农业科学院作物育种研究所黑龙江哈尔滨150086

利用水培试验研究不同形态氮素对不同类型小麦苗期生理特性及氮代谢酶的影响。结果表明,混合氮对于促进小麦的生长速率、提高叶片叶绿素含量及硝酸还原酶活性的效果最好,硝态氮次之,铵态氮效果最差。硝态氮显著提高了小麦叶片中硝态氮... 详细信息

利用水培试验研究不同形态氮素对不同类型小麦苗期生理特性及氮代谢酶的影响。结果表明,混合氮对于促进小麦的生长速率、提高叶片叶绿素含量及硝酸还原酶活性的效果最好,硝态氮次之,铵态氮效果最差。硝态氮显著提高了小麦叶片中硝态氮的含量,而铵态氮则显著提高了谷氨酰胺合成酶的活性。不同形态氮素对不同类型小麦的生长速度、叶片叶绿素含量和谷氨酰胺合成酶活性的影响没有显著差异,而强筋型小麦的硝酸还原酶活性显著高于中筋高产型小麦。

关键词：春小麦硝态氮铵态氮氮素

Moisture distribution in sludges based on different testing methods

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Journal of Environmental Sciences 2011年第5期23卷 875-880页

作者： wenyi Deng Xiaodong Li Jianhua yan Fei Wang Yong Chi Kefa Cen School of Environmental Science and Engineering Donghua University Shanghai 201620 China. E-mail: dengwy@*** State Key Laboratory of Clean Energy Utilization Institute for Thermal Power Engineering Zhejiang University Hangzhou 310027 China

Moisture distributions in municipal sewage sludge, printing and dyeing sludge and paper mill sludge were experimentally studied based on four different methods, i.e., drying test, thermogravimetric-differential thermal analysis （TG-DTA） test, thermogravimetric- differential scanning calorimetry （TG-DSC） test and water activity test. The results indicated that the moistures in the mechanically dewatered sludges were interstitial water, surface water and bound water. The interstitial water accounted for more than 50% wet basis （wb） of the total moisture content. The bond strength of sludge moisture increased with decreasing moisture content, especially when the moisture content was lower than 50% wb. Furthermore, the comparison among the four different testing methods was presented. The drying test was advantaged by its ability to quantify free water, interstitial water, surface water and bound water; while TG-DSC test, TG-DTA test and water activity test were capable of determining the bond strength of moisture in sludge. It was found that the results from TG-DSC and TG-DTA test are more persuasive than water activity test.

关键词： moisture distribution sludge bond strength

航天诱变与杂交相结合选育小麦新品种龙辐麦19

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作物杂志 2011年第4期 125-126,133页

作者：孙岩王广金张宏纪刘录祥闫文义刘东军赵林姝郭怡璠杨淑萍黑龙江省农业科学院作物育种研究所黑龙江哈尔滨150086 中国农业科学院作物科学研究所北京100081

龙辐麦19是黑龙江省农业科学院作物育种研究所用"九三3u90×九三少"杂交后代纯系材料经航天诱变的SP4代为母本,以优质品种龙麦26为父本杂交,按系谱法选育而成的高产、优质、抗病的小麦新品种。2011年2月通过黑龙江省农作物品种审定委员... 详细信息

龙辐麦19是黑龙江省农业科学院作物育种研究所用"九三3u90×九三少"杂交后代纯系材料经航天诱变的SP4代为母本,以优质品种龙麦26为父本杂交,按系谱法选育而成的高产、优质、抗病的小麦新品种。2011年2月通过黑龙江省农作物品种审定委员会审定命名推广。

关键词：春小麦航天诱变龙辐麦19 高产

小麦抗赤霉病突变体的选育及RAPD分子验证

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核农学报 1999年第4期13卷 202-205页

作者：孙光祖李忠杰李希臣王广金唐凤兰张月学阎文义孙德全孙岩黑龙江省农业科学院作物育种研究所

利用辐射诱变、组织培养和细胞筛选相结合的方法选育出了抗赤霉病突变体９２ｋ８０９。该突变体不仅抗赤霉病能力强，且抗性稳定，同时具有优良的农艺性状，比原亲本增产４９％。经ＲＡＰＤ技术检测，所用的１２８个引物中有１个引物... 详细信息

利用辐射诱变、组织培养和细胞筛选相结合的方法选育出了抗赤霉病突变体９２ｋ８０９。该突变体不仅抗赤霉病能力强，且抗性稳定，同时具有优良的农艺性状，比原亲本增产４９％。经ＲＡＰＤ技术检测，所用的１２８个引物中有１个引物（ＯＰＹ１５）在抗病突变体９２ｋ８０９和赤霉病抗原苏麦３号中得到了相同的扩增产物。初步认为该片段与赤霉病的抗性有关。

关键词：小麦抗赤霉病突变体选育 RAPD技术辐射诱变

Amine-functionalized SiO2 nanodot-coated layered double hydroxide nanocomposites for enhanced gene delivery

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Nano Research 2015年第2期8卷 682-694页

作者： Li Li wenyi Gu Jian Liu Shiyu yan Zhi Ping Xu Australian Institute for Bioengineering and Nanotechnology The University of Queensland Brisbane QLD 4072 Australia Department of Chemical Engineering Curtin University Perth WA 6845 Australia

In this work a novel strategy has been developed to prepare well-dispersed amine-functionalized SiO2 nanodot-coated layered double hydroxide nano- composite （NH2-SiO2@LDH） via electrostatic interactions and condensation of （3-aminopropyl）triethoxysilane （APTES）. This nanocomposite system is well dispersed in culture media and phosphate buffered saline, and exhibits low cytotoxicity and good biocompatibility. The fluorescence microscopy images and flow cytometry data indicate that such an NH2-SiO2@LDH nanocomposite is able to efficiently deliver small interfering RNA （siRNA） into the U2OS cell line to inhibit cell proliferation. Thus, NH2-SiOR@LDH nanocomposite has a great potential as a nanocarrier for efficient gene delivery.

关键词： layered double hydroxide(LDH) functionalization self-assembl3~gene delivery

A Large-Scale Cross-Sectional Study on Mental Health Status Among Children and Adolescents - Jiangsu Province, China, 2022

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China CDC weekly 2023年第32期5卷 710-714页

作者： Xin Wang yan Wang Xiyan Zhang wenyi yang Jie yang Department of Child and Adolescent Health Promotion Jiangsu Provincial Center for Disease Control and PreventionNanjing CityJiangsu ProvinceChina

What is already known about this topic?The prevalence of mental health disorders among children and youth in China stands at 17.5%,a figure that has been gradually escalating over recent *** surge has led to substantial psychological distress and financial strain for both families and the wider *** is added by this report?This study boasts an expansive geographical scope by covering the Jiangsu Province extensively and utilizing a substantial sample *** investigation was conducted on the prevalence and risk factors,which included family type and health behaviors,of depressive,anxiety,and stress *** are the implications for public health practice?The mental health status of children and adolescents should be of great concern to the ***,it is important that public health measures are implemented to mitigate the development of depressive *** measures may include regular screening procedures and the implementation of proven interventions.

关键词： measures utilizing Jiangsu

Shared Gene Regulation during Human Somatic Cell Reprogramming

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Journal of Genetics and Genomics 2012年第12期39卷 613-623页

作者： Xiang Wang Xuesong Chen Huijun Zhang wenyi Qin yan Xue Fanyi Zeng Laboratory of Developmental Biology Institute of Medical ScienceSchool of MedicineShanghai Jiao Tong University Shanghai Institute of Medical Genetics Shanghai Children's HospitalShanghai Jiao Tong University

Human induced pluripotent stem （iPS） cells have the ability to differentiate into all somatic cells and to maintain unlimited self- renewal. Therefore, they have great potential in both basic research and clinical therapy for many diseases. To identify potentially universal mechanisms of human somatic cell reprogramming, we studied gene expression changes in three types of cells undergoing reprogramming. The set of 570 genes commonly regulated during induction of iPS cells includes known embryonic stem （ES） cell markers and pluripotency related genes. We also identified novel genes and biological categories which may be related to somatic cell reprogramming. For example, some of the down-regulated genes are predicted targets of the pluripotency microRNA cluster miR302/367, and the proteins from these putative target genes interact with the stem cell pluripotency factor POU5F1 according to our network analysis. Our results identified candidate gene sets to guide research on the mechanisms operating during somatic cell reprogramming.

关键词： Somatic cell reprogramming Human iPS cells Gene expression profile Network analysis

大兴安岭南段乌苏图如地区锡多金属矿地质特征及找矿潜力

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金属矿山 2024年第8期 124-132页

作者：闫红圃李瑞强刘文毅孙国峰张得恩张沁妍河南省地质研究院河南郑州450016 河南省金属矿产成矿地质过程与资源利用重点实验室河南郑州450016 河南省金属矿产深部控矿与成矿机制院士工作站河南郑州450016

大兴安岭地区是我国重要的锡成矿带之一,乌苏图如地区位于大兴安岭南段,西伯利亚板块和华北板块之间的增生造山带上,属突泉—林西华力西、燕山期铁(锡)、铜、铅、锌、银、铌钽三级成矿带。为查明该区成矿规律及找矿潜力,通过综合开展1∶... 详细信息

大兴安岭地区是我国重要的锡成矿带之一,乌苏图如地区位于大兴安岭南段,西伯利亚板块和华北板块之间的增生造山带上,属突泉—林西华力西、燕山期铁(锡)、铜、铅、锌、银、铌钽三级成矿带。为查明该区成矿规律及找矿潜力,通过综合开展1∶5万矿产地质调查、1∶5万水系沉积物测量、1∶5万高精度磁测及1∶1万地质草测、1∶2.5万土壤测量、1∶1万地化剖面测量、1∶1万激电中梯剖面测量、激电测深、槽探等工作,对区域成矿地质特征及成矿潜力进行了研究。结果表明:①区内以锡、铅、锌、铜、银等内生金属矿为主,新发现矿(化)点21处;②1∶5万水系沉积物测量圈出化探单元素异常438个,综合异常57个;③1∶5万高精度磁测圈定规模较大的磁异常34个,区内矿(化)点多处于正磁异常区与负磁异常区之间的梯度带上,是寻找多金属矿的有利部位;④矿床成因类型为热液型和接触交代型,主要成矿期为华力西成矿期和燕山成矿期,燕山期为锡多金属矿的主要成矿期;⑤区内矿产分布严格受控于地质构造、岩体以及地层,在横向分布上呈NE向带状展布,由北向南共5个矿带;⑥提交各类找矿靶区16处,其中以锡为主要矿种或伴生矿种的找矿靶区7个。

关键词：锡多金属矿地球物理地球化学成矿规律找矿潜力