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Regulation of Virus Replication and T Cell Homeostasis by N^6-Methyladenosine

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Virologica Sinica 2019年第1期34卷 22-29页

作者： Jing Yang Hong Wang wenyan zhang Institute of Virology and AIDS Research The First Hospital of Jilin University

RNA modifications are abundant in eukaryotes, bacteria, and archaea. N^6-methyladenosine(m^6A), a type of RNA modification mainly found in messenger RNA(mRNA), has significant effects on the metabolism and function of m RNAs. This modification is governed by three types of proteins, namely methyltransferases as ‘‘writers' ', demethylases as ‘‘erasers' ',and specific m^6A-binding proteins(YTHDF1-3) as ‘‘readers' '. Further, it is important for the regulation of cell fate and has a critical function in many biological processes including virus replication, stem cell differentiation, and cancer development, and exerts its effect by controlling gene expression. Herein, we summarize recent advances in research on m^6A in virus replication and T cell regulation, which is a rapidly emerging field that will facilitate the development of antiviral therapies and the study of innate immunity.

关键词： RNA modification N^6-methyladenosine (m^6A) Virus replication T cell homeostasis

Effects of dissolved oxygen and nutrients from the Kuroshio on hypoxia off the Changjiang River estuary

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Journal of Oceanology and Limnology 2022年第2期40卷 515-529页

作者： Di TIAN Feng ZHOU wenyan zhang Han zhang Xiao MA Xinyu GUO State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China Observation and Research Station of Yangtze River Delta Marine Ecosystems Ministry of Natural ResourcesZhoushan 316022China Ocean College Zhejiang UniversityZhoushan 316021China Institute of Coastal Research Helmholtz-Zentrum GeesthachtGeesthacht 21502Germany Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519082China Center for Marine Environmental Study Ehime UniversityMatsuyama 790-8577Japan

The intrusion of the Kuroshio into the East China Sea(ECS)aff ects the development of hypoxia off the Changjiang(Yangtze)River estuary;however,quantitative analysis of its impacts is *** this study,the Regional Ocean Modeling Systems(ROMS)model coupled with the Carbon,Silicate and Nitrogen Ecosystem(CoSiNE)model was used to investigate the relative importance of dissolved oxygen(DO)and diff erent nutrients(silicate,nitrate,and phosphate)in the Kuroshio on hypoxia in the *** show that changes in DO concentrations in the Kuroshio modify the distribution and intensity of hypoxia through direct onshore transport by hydrodynamic *** increase in Kuroshio DO concentration by 25%or 50%would result in a decrease of the maximum hypoxia extent(MHE)in the ECS by 76%or 86%,respectively,while a 25%decrease in Kuroshio DO would increase the MHE by up to 219%.The contribution of DO in the Taiwan Strait is almost *** contrast to Kuroshio DO,nutrients aff ect hypoxia in the ECS through onshore transport by hydrodynamic and biochemical *** in phosphate and nitrate concentrations by 25%in the Kuroshio would change the MHE by up to 30%and 18%,respectively,accompanied by apparent changes in surface chlorophyll-a *** eff ect of silicate on hypoxia is negligible because a 25%change in silicate concentrations in the Kuroshio would result in less than 1%change in the *** results reveal a hierarchical rank of importance for environmental variables in the Kuroshio(i.e.,DO>phosphate>nitrate>silicate)in modifying the development of hypoxia in the ECS.

关键词： hypoxia Kuroshio Changjiang River estuary East China Sea dissolved oxygen nutrient

六味地黄丸调节肠道微生物组平衡抑制卵巢早衰小鼠的氧化应激

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中国组织工程研究 2025年第11期29卷 2285-2293页

作者：仲佳雯姜波张文燕李晓荣秦岭高婷宁夏医科大学中医学院宁夏回族自治区银川市750004 宁夏医科大学生育力保持重点实验室宁夏回族自治区银川市750004 宁夏医科大学少数民族医药现代化教育部重点实验室宁夏回族自治区银川市750004 宁夏医科大学总医院宁夏回族自治区银川市750000

背景:研究表明,卵巢早衰患者肠道菌群结构发生变化,肠道菌群失调可能是卵巢早衰发生的重要机制之一。目的:探讨六味地黄丸对环磷酰胺诱导卵巢早衰小鼠氧化应激和肠道微生物群的影响。方法:45只雌性ICR小鼠随机分为3组:空白组(正常小鼠)... 详细信息

背景:研究表明,卵巢早衰患者肠道菌群结构发生变化,肠道菌群失调可能是卵巢早衰发生的重要机制之一。目的:探讨六味地黄丸对环磷酰胺诱导卵巢早衰小鼠氧化应激和肠道微生物群的影响。方法:45只雌性ICR小鼠随机分为3组:空白组(正常小鼠)、模型组(卵巢早衰小鼠)、六味地黄丸组,后2组小鼠一次性腹腔注射环磷酰胺(120 mg/kg)制备卵巢早衰小鼠模型,造模成功后六味地黄丸组小鼠连续灌胃六味地黄丸(1.56 g/kg)28 d,其余2组灌胃等量生理盐水28 d,灌胃结束后取材。每周记录小鼠体质量,计算卵巢指数,苏木精-伊红染色观察小鼠卵泡的发育情况,ELISA法检测血清抗苗勒管激素、雌二醇、促卵泡激素、超氧化物歧化酶、谷胱甘肽过氧化物酶和丙二醛水平,并通过16S rDNA测序检测所有小鼠的肠道微生物组。结果与结论:(1)模型组小鼠的毛发疏松,活力及抓力下降,体质量几乎无增长,卵巢指数下降;六味地黄丸治疗后小鼠的体质量高于模型组,卵巢指数也随之升高(P<0.05)。(2)模型组小鼠动情周期紊乱;六味地黄丸能恢复卵巢早衰小鼠的发情周期,减少闭锁卵泡数量。(3)模型组小鼠血清中促卵泡激素、丙二醛水平显著升高(P<0.01),血清雌二醇、抗苗勒管激素、超氧化物歧化酶和谷胱甘肽过氧化物酶水平显著下降(P<0.01);六味地黄丸组小鼠血清促卵泡激素、丙二醛水平显著降低(P<0.01),雌二醇、抗苗勒管激素、超氧化物歧化酶、谷胱甘肽过氧化物酶水平升高。(4)根据16S rDNA测序结果,六味地黄丸可调控肠道微生物群的丰度和多样性,可增加有益菌的相对丰度,KEGG通路分析显示,肠道微生物群与代谢途径、次生代谢产物的生物合成、不同环境中的微生物代谢、氨基酸的生物合成等通路受六味地黄丸的调控。(5)结论:肠道微生物组的改变可能是六味地黄丸治疗卵巢早衰的潜在机制之一。六味地黄丸能调节肠道微生物组结构,增加有益菌数量,减少有害菌数量,从而改善肠道微生态平衡。这种调节作用有助于降低氧化应激水平,进一步抑制卵巢早衰小鼠卵巢氧化应激。

关键词：六味地黄丸卵巢早衰肠道微生物组氧化应激

IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathway

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Virologica Sinica 2022年第5期37卷 676-684页

作者： Baisong Zheng Xiaolei Zhou Li Tian Jian Wang wenyan zhang Center for Pathogen Biology and Infectious Diseases Institute of Virology and AIDS ResearchKey Laboratory of Organ Regeneration and Transplantation of the Ministry of EducationThe First Hospital of Jilin UniversityChangchun130021China

Enterovirus 71(EV71) caused hand, foot and mouth disease(HFMD) is a serious threat to the health of young children. Although type I interferon(IFN-I) has been proven to control EV71 replication, the key downstream IFNstimulated gene(ISG) remains to be clarified and investigated. Recently, we found that 2’-5’-oligoadenylate synthetases 3(OAS3), as one of ISG of IFN-β1b, was antagonized by EV71 3C protein. Here, we confirm that OAS3is the major determinant of IFN-β1b-mediated EV71 inhibition, which depends on the downstream constitutive RNase L activation. 2’-5’-oligoadenylate(2-5A) synthesis activity deficient mutations of OAS3 D816A, D818A,D888A, and K950A lost resistance to EV71 because they could not activate downstream RNase L. Further investigation proved that EV71 infection induced OAS3 but not RNase L expression by IFN pathway. Mechanically, EV71 or IFN-β1b-induced phosphorylation of STAT1, but not STAT3, initiated the transcription of OAS3 by directly binding to the OAS3 promoter. Our works elucidate the immune regulatory mechanism of the host OAS3/RNase L system against EV71 replication.

关键词： Enterovirus 71(EV71) 20-50-oligoadenylate synthetases 3(OAS3) RNase L IFN-β1b JAK/STAT

EV713C protease cleaves host anti-viral factor OAS3 and enhances virus replication

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Virologica Sinica 2022年第3期37卷 418-426页

作者： Xiaolei Zhou Li Tian Jian Wang Baisong Zheng wenyan zhang Center for Infectious Diseases and Pathogen Biology Institute of Virology and AIDS ResearchKey Laboratory of Organ Regeneration and Transplantation of the Ministry of EducationThe First Hospital of Jilin UniversityJilin130021China

The global spread of enteroviruses(EVs)has become more frequent,severe and ***(IFN)I has been proved to control EVs by regulating IFN-stimulated genes(ISG)expression.20-50-oligoadenylate synthetases 3(OAS3)is an important ISG in the OAS/RNase L antiviral *** relationship between OAS3 and EVs is still ***,we reveal that OAS3,superior to OAS1 and OAS2,significantly inhibited EV71 replication in ***,EV71 utilized autologous 3C protease(3C^(pro))to cleave intracellular OAS3 and enhance viral ***,a human rhinovirus 3C protease inhibitor,completely abolished the cleavage of EV713C^(pro)on *** the proteolytically deficient mutants H40G,E71A,and C147G of EV713C^(pro)also lost the ability of OAS3 ***,the Q982-G983 motif in C-terminal of OAS3 was identified as a crucial 3C^(pro)cutting *** investigation indicated that OAS3 inhibited not only EV71 but also Coxsackievirus B3(CVB3),Coxsackievirus A16(CA16),Enterovirus D68(EVD68),and Coxsackievirus A6(CA6)***,unlike other four subtypes,CA163C^(pro)could not cleave *** key amino acids variation Ile36 and Val86 in CA163C^(pro)might result in weak and delayed virus replication of CA16 because of failure of OAS and 3AB *** works elucidate the broad anti-EVs function of OAS3,and illuminate a novel mechanism by which EV71 use 3C^(pro)to escape the antiviral effect of *** findings can be an important entry point for developing novel therapeutic strategies for multiple EVs infection.

关键词： Enteroviruses EV71 3C protease Cleavage Anti-viral factor OAS3

Effects of Panax notoginseng saponins in a rat model of Alzheimer's disease

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Neural Regeneration Research 2008年第1期3卷 37-40页

作者： Zhenguo Zhong Zeqiang Qu Yunping Bao Naiping Wang Fengfen zhang wenyan zhang Center of Research ＆ Development of New Drugs Guangxi Traditional Chinese Medical University Nanning 530001 Guangxi Zhuang Autonomous Region China Department of Pharmacology Guangxi Traditional Chinese Medical University Nanning 530001 Guangxi Zhuang Autonomous Region China

BACKGROUND： Modem pharmacological studies have demonstrated that Panax notoginseng saponins （PNS） can ameliorate and protect from neuropathological impairment. Whether PNS can improve the abnormality in memory and behavior of rats with Alzheimer＇s disease （AD） remains unclear. OBJECTIVE： Based on a Morris water maze test, this study aimed to measure improvements of spatial learning and memory by PNS in a rat model of AD, and to compare effects with huperzine A. DESIGN： A completely randomized grouping design, controlled animal experiment. SETTING： Center of Research ＆ Development of New Drugs, Guangxi Traditional Chinese Medical University. MATERIALS： Ninety healthy Wistar rats of both genders, 15-month-old （n =75） and 3-month-old rats as young controls （n =15）, were used for this study. The study was performed in accordance with animal ethics guidelines for the use and care of animals. PNS was provided by Weihe Pharmaceutical Co., Ltd （permission No. Z53021485, Yuxi, Yunan Province, China）. Morris water maze equipment was provided by the Institute of Physiology, Chinese Academy of Science. METHODS： This study was performed at the Center of Research ＆ Development of New Drugs, Guangxi Traditional Chinese Medical University from June 2003 to April 2005. Of the included rats, 15 healthy aged rats were randomly chosen as aged controls, and the remaining 60 aged rats were randomly divided into 4 groups with 15 rats in each： model group, PNS high- and low-dose groups, and an huperzine A group. Rats in the model group and the 3 treated groups were treated with intraperitoneal infusion of 9.6 g/L D-galactose （5 mL/kg） every day for 6 weeks successively to induce a subacute aging model. During week 7, animals received 1 μ L ibotenic acid （5 g/L） bilaterally into the nucleus basalis of Meynert to create a rat model of AD. The young and old rat controls received, in parallel, a corresponding volume of saline. Two weeks later, rats in the PNS high- and low-dose groups were gav

关键词： Alzheimer's disease Panax notoginseng saponins animal model Morris water maze learning memory

Depositional Characteristics and Formation Mechanisms of Deep-Water Canyon Systems along the Northern South China Sea Margin

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Journal of Earth Science 2020年第4期31卷 808-819页

作者： Hui Chen Xinong Xie Kainan Mao Yunlong He Ming Su wenyan zhang School of Marine Sciences Sun Yat-sen UniversityZhuhai 519082China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519082China Hubei Key Laboratory of Marine Geological Resources China University of GeosciencesWuhan 430074China School of Resources and Environmental Engineering Guizhou Institute of TechnologyGuiyang 550003China Institute of Coastal Research Helmholtz-Zentrum GeesthachtGeesthacht 21502Germany

Submarine canyon systems are sites for coarser clastic sediment accumulations in the deep-water domains, having the most potential for hydrocarbon reservoirs. Based on the interpretation of high resolution 2 D/3 D seismic and drilling data, depositional characteristics of three large deep-water canyon systems on the South China Sea northern margin have been analyzed. The Central Canyon System has a deep incision geomorphology extending from east to west, featured by distinct canyon segmentations, multi-provenance sediment supplies and multi-stage canyon fillings. The Pearl River Canyon System’s formation is closely related to the development of Pearl River Delta. Its vertical stacking and migrating canyon patterns have changed over time. The depositional architectures and evolution of the recent Penghu-Gaoping Canyon System respond to tectonic movements along the Taiwan-Luzon convergent continental margin. The main controlling factors of the formation and evolution of these three canyon systems include the tectonic setting, sediment supply, sea level change and paleo-geomorphology, among which the former two are dominant. The Penghu-Gaoping Canyon System formed along the subduction structural zone, directly indicating a typical tectonic origin. Numerous seismic data show that the Central Canyon and Pearl River Canyon systems are obviously affected by tectonics, associated local topography and sediment supply.

关键词： deep-water canyon system turbidite channel deep-water reservoir northern margin of South China Sea

Bacterial and archaeal community structures in the Arctic deepsea sediment

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Acta Oceanologica Sinica 2015年第2期34卷 93-113页

作者： LI Yan LIU Qun LI Chaolun DONG Yi zhang wenyan zhang Wuchang XIAO Tian Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Center for Transport Phenomena Energy Research Institute Shandong Academy of Sciences State Field Research Station of Jiaozhou Bay Marine Ecology Institute of Oceanology Chinese Academy of Sciences

Microbial community structures in the Arctic deep-sea sedimentary ecosystem are determined by organic matter input, energy availability, and other environmental factors. However, global warming and earlier ice-cover melting are affecting the microbial diversity. To characterize the Arctic deep-sea sediment microbial diversity and its relationship with environmental factors, we applied Roche 454 sequencing of 16 S r DNA amplicons from Arctic deep-sea sediment sample. Both bacterial and archaeal communities＇ richness, compositions and structures as well as taxonomic and phylogenetic affiliations of identified clades were characterized. Phylotypes relating to sulfur reduction and chemoorganotrophic lifestyle are major groups in the bacterial groups; while the archaeal community is dominated by phylotypes most closely related to the ammonia-oxidizing Thaumarchaeota（96.66%） and methanogenic Euryarchaeota（3.21%）. This study describes the microbial diversity in the Arctic deep marine sediment（〉3 500 m） near the North Pole and would lay foundation for future functional analysis on microbial metabolic processes and pathways predictions in similar environments.

关键词： Arctic deep-sea sediment microbial community structure pyrosequencing

Effect of Panax notoginseng saponins on the expression of beta-amyloid protein in the cortex of the parietal lobe and hippocampus, and spatial learning and memory in a mouse model of senile dementia

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Neural Regeneration Research 2008年第12期3卷 1297-1303页

作者： Zhenguo Zhong Dengpan Wu Liang Lu Jinsheng Wang wenyan zhang Zeqiang Qu Center of Research ＆ Development of New Drugs Guangxi College of Traditional Chinese Medicine Nanning 530001 Guangxi ZhuangAutonomous Region China

BACKGROUND： The pharmacological actions of Panax notoginseng saponins （PNS） lie in removing free radicals, anti-inflammation and anti-oxygenation. It can also improve memory and behavior in rat models of Alzheimer＇s disease. OBJECTIVE： Using the Morris water maze, immunohistochemistry, real-time PCR and RT-PCR, this study aimed to measure improvement in spatial learning, memory, expression of amyloid precursor protein （App） and β -amyloid （A β ）, to investigate the mechanism of action of PNS in the treatment of AD in the senescence accelerated mouse-prone 8 （SAMP8） and compare the effects with huperzine A. DESIGN, TIME AND SETTING： A completely randomized grouping design, controlled animal experiment was performed in the Center for Research ＆ Development of New Drugs, Guangxi Traditional Chinese Medical University from July 2005 to April 2007. MATERIALS： Sixty male SAMP8 mice, aged 3 months, purchased from Tianjin Chinese Traditional Medical University of China, were divided into four groups： PNS high-dosage group, PNS low-dosage group, huperzine A group and control group. PNS was provided by Weihe Pharmaceutical Co., Ltd. （batch No.： Z53021485, Yuxi, Yunan Province, China）. Huperzine A was provided by Zhenyuan Pharmaceutical Co., Ltd. （batch No.： 20040801, Zhejiang, China）. METHODS： The high-dosage group and low-dosage group were treated with 93.50 and 23.38 mg/kg PNS respectively per day and the huperzine A group was treated with 0.038 6 mg/kg huperzine A per day, all by intragastric administration, for 8 consecutive weeks. The same volume of double distilled water was given to the control group. MAIN OUTCOME MEASURES： After drug administration, learning and memory abilities were assessed by place navigation and spatial probe tests. The recording indices consisted of escape latency （time-to-platform）, and the percentage of swimming time spent in each quadrant. The number of A β 1-40, A β 1-42 and App immunopositive neurons in the brains of SAMP8 mice was ana

关键词： Alzheimer's disease Panax notoginseng saponins learning and memory β -amyloid precursor protein 1-40 β -amyloid precursor protein 1-42 amyloid β -peptide synaptophysin senescence accelerated mouse-prone 8