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The circular RNA circCPE regulates myoblast development by sponging miR-138

Journal of Animal Science and Biotechnology 2022年第1期13卷 66-81页

作者： wenxiu ru Ao Qi Xuemei Shen Binglin Yue Xiaoyan Zhang Jian Wang Hui Cao Hong Chen Key laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100ShaanxiChina Shaanxi Kingbull Livestock co. LTDYangling 712100ShaanxiChina

Background:Skeletal muscle development,a long-term and complex process,is controlled by a set of the myogenic *** RNAs(circRNAs),a class of noncoding RNA,have been shown to regulate various biological *** studies indicate circRNAs may be involved in myogenesis,but the role and regulatory mechanism of circRNAs in myogenesis is largely *** the present study,circCPE was firstly found to promote the bovine myoblast proliferation and inhibit cell apoptosis and differentiation by influencing the expression of FOXC1 in a miR138-mediated *** in vivo experiments revealed that overexpression of circCPE attenuates skeletal muscle ***:We identified a novel circular RNA circCPE by analyzing circRNAs sequencing data of bovine muscle *** verification,RNase R treatment and Actinomycin D treatment confirmed the circular nature of circCPE in bovine *** assays showed that overexpression of circCPE could inhibit bovine myoblast apoptosis and differentiation,as well as facilitate cell ***,in vivo experiments revealed that overexpression of circCPE attenuates skeletal muscle *** consideration of circRNA action as miRNAs sponge,we found that circCPE harbors miR-138 binding sites and absorbed ***,the rescue experiments showed that the overexpression of circCPE can counteract the inhibitory effect of miR-138 on the cell proliferation and the accelerated effects on the differentiation and ***,we found that circCPE sequester the inhibitory effect of miR-138 on FOXC1 so as to involve in ***:Collectively,we constructed a novel circCPE/miR-138/FOXC1 regulatory network in bovine myogenesis,which further provide stronger evidence that circRNA involved in muscle development acting as miRNA sponge.

关键词： Apoptosis Bovine CircRNA Differentiation MiR-138 Proliferation

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The Effect of the Cyp19a1 Gene Methylation Modification on Temperature-dependent Sex Determination of Reeves' Turtle(Mauremys reevesii)

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Asian Herpetological Research 2017年第3期18卷 213-220页

作者： wenxiu ru Liushuai HUA Yufeng WEI Weiye LI Dainan CAO Yan GE Hong CHEN Xianyong LAN Shiping GONG Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Animal Science and TechnologyNorthwest A&F University Guangdong Key Laboratory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and UtilizationGuangdong Institute of Applied Biological Resources Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation Institute of Animal Husbandry and Veterinary ScienceHenan Academy of Agricultural Sciences

Temperature-dependent sex determination（TSD） is a type of environmental sex determination in which the sex of the embryos depends on the ambient temperature; however,the molecular mechanisms governing this process remain ***,encoded by the cyp19a1 gene,which converts androgens into estrogens in animals,was considered to be the key gene for *** this study,the 5＇-flanking region of the cyp19a1 gene in Reeves＇ turtle（Mauremys reevesii） was cloned,and the promoter region was identified using the luciferase reporter *** the eggs of Reeves＇ turtle were incubated at different temperatures（26°C： male-biased temperature; 29°C： non-sex-biased temperature and 32°C： female-biased temperature）.During the thermosensitive period,the adrenal kidney gonad complexes（AKG） were *** methylation analysis of the AKG samples showed that the promoter region of the cyp19a1 gene was significantly de-methylated in the female-biased temperature regime（P＆lt;0.01）.Quantitative analysis of the cyp19a1 gene and estrogen by q PCR and Elisa assay showed that the expression level of the cyp19a1 gene and estrogen content were both upregulated significantly at the female-biased temperature（P＆lt;0.01）.These results indicated that the de-methylation response of the cyp19a1 gene to incubation temperature,especially at the female-biased temperature,could lead to temperature-specific expression of aromatase and increased estrogen levels,which may further determine gonadal development in Reeves＇ *** findings provide insights into the genetic mechanisms underlying TSD.

关键词： Reeves' turtle Mauremys reevesii temperature-dependent sex determination (TSD) cyp19a1 gene,methylation

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circSVIL regulates bovine myoblast development by inhibiting STAT1 phosphorylation

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Science China(Life Sciences) 2022年第2期65卷 376-386页

作者： Binglin Yue Haiyan Yang Jiyao Wu Jian Wang wenxiu ru Jie Cheng Yongzheng Huang Xianyong Lan Chuzhao Lei Hong Chen Key laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China

Circular RNAs(circRNAs),a novel class of non-coding RNAs with a loop structure,have recently been shown to participate in various pathophysiological ***,the precise role of circRNAs in myoblasts remains *** this report,circSVIL was screened and identified from our previous sequencing analysis;we then performed gain-and loss-of-function experiments on bovine myoblasts by CCK8,EdU,flow cytometry,qRT-PCR,and Western *** results indicate that circSVIL facilitates bovine myoblast proliferation and inhibits cell *** mechanism assays such as bioinformatics prediction,RNA immunoprecipitation(RIP),and cytoplasmic separation,we demonstrate that circSVIL could interact with STAT1 and inhibit STAT1 phosphorylation,thereby restraining STAT1’s nuclear translocation and affecting its downstream signal *** results may elucidate a new regulatory pathway for bovine skeletal muscle development.

关键词： circSVIL STAT1 skeletal muscle

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ru-doped functional porous materials for electrocatalytic water splitting

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Nano Research 2024年第3期17卷 982-1002页

作者： Chongao Tian rui Liu Yu Zhang wenxiu Yang Bo Wang Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials Advanced Technology Research Institute(Jinan)Advanced Research Institute of Multidisciplinary ScienceSchool of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Library Beijing Institute of TechnologyBeijing 100081China

Electrolytic water splitting(EWS)is an attractive and promising technique for the production of hydrogen ***,the sluggish kinetic rate of hydrogen/oxygen evolution reactions leads to a high overpotential and low energy *** to date,Pt/Ir-based nanocatalysts have become the state-of-the-art EWS catalysts,but disadvantages such as high cost and low earth abundance greatly limit their applications in EWS *** an attractive candidate for the Pt/Ir catalysts,series of ru-based nanomaterials have aroused much attention for their low price,Pt-like hydrogen bond strength,and high EWS *** particular,ru-doped functional porous materials have been becoming one of the most representative EWS catalysts,which can not only achieve the dispersion and adjustment for active ru sites,but also simultaneously solve the problems of mass transfer and catalytic conversion in *** this review,the design and preparation strategies of ru-doped functional porous materials toward EWS in recent years are summarized,including ru-doped metal organic frameworks(MOFs),ru-doped porous organic polymers(POPs),and their ***,detailed structure–activity relationships induced by the tuned geometric/electronic structures of ru-doped functional porous materials are further depicted in this *** but not least,the challenges and perspectives of ru-doped functional porous materials catalysts are reasonably proposed to provide fresh ideas for the design of ru-based EWS catalysts.

关键词： ru-based catalyst functional porous materials electrolytic water splitting oxygen evolution reaction hydrogen evolution reaction

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Synergizing high valence metal sites and amorphous/crystalline interfaces in electrochemical reconstructed CoFeOOH heterostructure enables efficient oxygen evolution reaction

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Nano Research 2022年第10期15卷 8857-8864页

作者： Xiangjian Liu rui Liu Jinming Wang Yarong Liu Liuhua Li wenxiu Yang Xiao Feng Bo Wang Key Laboratory of Cluster Science Ministry of Education Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion MaterialsAdvanced Technology Research Institute(Jinan)Advanced Research Institute of Multidisciplinary ScienceSchool of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081China

Cobalt hydroxide nanosheet is among the most popular oxygen evolution reaction(OER)catalyst yet still suffers from sluggish catalytic kinetics,limited activity,and poor ***,an efficient in situ electrochemical reconstructed CoFe-hydroxides derived OER electrocatalyst was *** introduction of Fe promoted the transformation of Co^(2+)into Co^(3+)in CoFehydroxides nanosheet,along with the formation of abundant amorphous/crystalline *** for the retained nanosheet microstructure,high valence Co^(3+)and Fe^(3+)species,and the amorphous/crystalline heterostructure interfaces,the as-designed electrochemical reconstructed CoFeOOH nanosheet/Ni foam(CoFeOOHNS/NF)electrode delivers 100 mA·cm^(−2) in alkaline at an overpotential of 275 mV and can stably electrocatalyze water oxidation for at least 35 h at 100 mA·cm^(−2).Meanwhile,the alkaline full water splitting electrolyzer achieves a current density of 10 mA·cm^(−2) only at 1.522 V for CoFeOOHNS/NF‖Pt/C/NF,which is much lower than that of ru/C/NF‖Pt/C/NF(1.655 V@10 mA·cm^(−2)).This work paves the way for in-situ synergetic modification engineering of electrochemical active components.

关键词： electrochemical reconstruction high valance transition metal amorphous heterostructure interfaces electrochemical oxygen evolution reaction

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Amorphous ru nanoclusters onto Co‐doped 1D carbon nanocagesenables efficient hydrogen evolution catalysis

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Chinese Journal of Catalysis 2022年第1期43卷 110-116页

作者： wenxiu Yang Weiyu Zhang rui Liu Fan Lv Yuguang Chao Zichen Wang Shaojun Guo School of Materials Science & Engineering Peking University Beijing 100871 China Advanced Research Institute of Multidisciplinary Science Advanced Technology Research Institute (Jinan) Key Laboratory of Cluster Science School of Chemistry and Chemical Engineering Beijing Institute of Technology Beijing 100081 China

析氢反应是电解水产制氢的关键反应之一.在碱性条件下,由于催化剂表面与反应过程中产生的氧物种、氢物种与催化剂的吸附未处于最佳状态,析氢反应动力学往往比较缓慢,比在酸性条件下慢2–3个数量级.目前,铂基纳米催化剂被认为是最优的析... 详细信息

析氢反应是电解水产制氢的关键反应之一.在碱性条件下,由于催化剂表面与反应过程中产生的氧物种、氢物种与催化剂的吸附未处于最佳状态,析氢反应动力学往往比较缓慢,比在酸性条件下慢2–3个数量级.目前,铂基纳米催化剂被认为是最优的析氢催化剂,但因价格昂贵、稳定性较差,限制了其在电解水器件上的大规模应用.因此,设计一种价格较为低廉、活性高和稳定性好的碱性析氢催化剂尤为必要.钌作为铂族金属之一,其价格约为铂的三分之一,但其与氢的结合能却与铂类似.因此,钌基催化剂被认为是有望替代铂作为析氢催化剂.本文结合模板法、静电纺丝和碳化热解策略构筑了一种负载无定型钌纳米簇的原子级钴掺杂一维碳纳米笼催化剂,有效提升了碱性条件下析氢反应性能.该催化剂具有较高的比表面积,丰富的缺陷结构,原子级分散的金属Co掺杂以及无定型钌纳米簇结构,并在碱性条件下催化析氢反应时,表现出了低起始过电位,低Tafel斜率(62 mV dec–1)和高稳定性.X射线衍射(XRD)结果表明,该催化剂中钌和钴以无定型形式存在.选区电子衍射结果表明了无定型结构的存在,并且钌以纳米簇形式存在,其平均粒径为1.48 nm.X射线光电子能谱结果表明该催化剂含有较高的氮掺杂,拉曼光谱证明了其存在碳缺陷结构.进一步通过X射线近边吸收谱和扩展边精细结构谱研究了钌和钴的配位环境,结果发现,钌带轻微正电荷,并且存在ru–ru键;钴带正电荷,价态介于0与+2价之间,其主要与N配位.在1 mol/L氢氧化钾电解液中,该杂化催化剂的电催化活性明显高于没有钴掺杂的催化剂以及商业化铂/碳催化剂.在电流密度为10 mAcm–2时,过电位仅为40 mV,在过电位为100 mV时,该催化剂的质量催化活性达到了3.77 mAμgru–1,远超商业化铂/碳(2.01 mAμgru–1)和钌/碳(1.66 mAμgru–1).该催化剂也表现出了优异的析氢催化稳定性, 5000圈循环后, 10 mAcm–2下的过电位仅增加了2 mV,在恒电流测试中, 10 h后电位仅下降了95 mV.

关键词：无定型钌纳米簇单原子富缺陷碳析氢反应电催化

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基于COSMO-RS方法筛选离子液体分离乙酸乙酯-乙腈共沸物

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化工学报 2019年第1期70卷 146-153页

作者：张志刚张德彪张亲亲张弢杨茹李文秀沈阳化工大学化学工程学院辽宁沈阳110142

采用COSMO-RS方法探究了不同离子液体在分离乙酸乙酯-乙腈共沸物系中的应用;通过预测值与实验值的对比,验证了COSMO-therm X预测离子液体分离效果的准确性。离子液体的选择性和不同离子液体对乙酸乙酯-乙腈近沸点处相对挥发度的影响为指... 详细信息

采用COSMO-RS方法探究了不同离子液体在分离乙酸乙酯-乙腈共沸物系中的应用;通过预测值与实验值的对比,验证了COSMO-therm X预测离子液体分离效果的准确性。离子液体的选择性和不同离子液体对乙酸乙酯-乙腈近沸点处相对挥发度的影响为指标,计算和分析了17种阳离子和13种阴离子组成的221种离子液体对乙酸乙酯-乙腈共沸物系的分离效果的影响规律。结果表明,含有[OAc]-和[Cl]-的离子液体对于乙酸乙酯-乙腈混合物的分离具有更好的促进效果,而阳离子的变化对于乙酸乙酯-乙腈混合物分离的促进效果差异较小。应用表面电荷密度分布(σ-profile)进一步研究了阴离子[OAc]-和[Cl]-对共沸物的影响,研究发现,阴离子[OAc]-和[Cl]-对乙酸乙酯-乙腈的分离效果影响顺序为[OAc]->[Cl]-;综合筛选结果,得出离子液体[BMIM][OAc]有望在乙酸乙酯-乙腈共沸混合物分离过程中成为一种高效的萃取剂。

关键词：乙酸乙酯乙腈离子液体 COSMO-RS模型选择性

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广义Pochhammer-Chree方程的显式精确孤波解

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应用数学和力学 1999年第6期20卷 625-632页

作者：张卫国马文秀长沙铁道学院数理力学系复旦大学数学系

首先对广义Ｐｏｃｈｈａｍｍｅｒ＿Ｃｈｒｅ方程（ＰＣ方程）ｕｔ－ｕｔｘｘ＋ｒｕｘｘｔ－（ａ１ｕ＋ａ２ｕ２＋ａ３ｕ３）ｘｘ＝０（ｒ≠０）（Ⅰ）的孤波解ｕ（ξ）建立了公式∫＋∞－∞［ｕ′（ξ）］２ｄξ＝１１２ｒｖ（Ｃ＋－Ｃ... 详细信息

首先对广义Ｐｏｃｈｈａｍｍｅｒ＿Ｃｈｒｅ方程（ＰＣ方程）ｕｔ－ｕｔｘｘ＋ｒｕｘｘｔ－（ａ１ｕ＋ａ２ｕ２＋ａ３ｕ３）ｘｘ＝０（ｒ≠０）（Ⅰ）的孤波解ｕ（ξ）建立了公式∫＋∞－∞［ｕ′（ξ）］２ｄξ＝１１２ｒｖ（Ｃ＋－Ｃ－）３［３ａ３（Ｃ＋＋Ｃ－）＋２ａ２］·由此推知：广义ＰＣ方程（Ⅰ）不可能有钟状孤波解，只可能有扭状孤波解；而广义ＰＣ方程ｕｔ－ｕｔｘｘ－（ａ１ｕ＋ａ２ｕ２＋ａ３ｕ３）ｘｘ＝０（Ⅱ）可能既有钟状孤波解又有渐近值满足３ａ３（Ｃ＋＋Ｃ－）＋２ａ２＝０的扭状孤波解·进一步求出了广义ＰＣ方程（Ⅰ）的扭状孤波解。

关键词：非线性发展方程广义孤波解精确解 P-C方程

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乙酸乙酯-乙腈-1-丁基-3-甲基咪唑磷酸二丁酯盐物系等压气液相平衡数据的测定及关联

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石油化工 2018年第9期47卷 951-955页

作者：张志刚张德彪李文秀杨茹杨树元张亲亲沈阳化工大学化学工程学院辽宁沈阳110142 内蒙古科右前旗乌兰河自然保护区管委会内蒙古科右前旗137400

在101.3 kPa下,对乙酸乙酯-乙腈-1-丁基-3-甲基咪唑磷酸二丁酯盐([BMIM][DBP])三元物系的等压气液相平衡数据进行测定,并与Aspen软件中NRTL模型进行关联,探讨了[BMIM][DBP]等多种离子液体对乙酸乙酯-乙腈二元物系的气液相平衡的影响。... 详细信息

在101.3 kPa下,对乙酸乙酯-乙腈-1-丁基-3-甲基咪唑磷酸二丁酯盐([BMIM][DBP])三元物系的等压气液相平衡数据进行测定,并与Aspen软件中NRTL模型进行关联,探讨了[BMIM][DBP]等多种离子液体对乙酸乙酯-乙腈二元物系的气液相平衡的影响。实验结果表明,[BMIM][DBP]的加入会产生显著的盐析效应,[BMIM][DBP]含量越高效果越明显,当[BMIM][DBP]摩尔分数达到0.05时可消除乙酸乙酯-乙腈的共沸现象;NRTL模型对气液相平衡数据进行关联的效果良好;[BMIM][DBP]更适于作为分离乙酸乙酯-乙腈的夹带剂。

关键词：气液相平衡离子液体乙酸乙酯乙腈 NRTL模型

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《入彭蠡湖口》“骤”释义补正

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现代语文 2021年第5期 21-25页

作者：刘文秀福建师范大学文学院福建福州350007

关于谢灵运《入彭蠡湖口》诗“洲岛骤回合”中“骤”的解释,学界有不同的看法。总括起来,主要有“急遽”义、“屡次”义两种观点。“屡次”义为频数层面的意义,“急遽”义为速度层面的意义,二者具有一定的逻辑联系。通过词义系统及文献... 详细信息

关于谢灵运《入彭蠡湖口》诗“洲岛骤回合”中“骤”的解释,学界有不同的看法。总括起来,主要有“急遽”义、“屡次”义两种观点。“屡次”义为频数层面的意义,“急遽”义为速度层面的意义,二者具有一定的逻辑联系。通过词义系统及文献材料,对两种语义的合理性进行探讨。同时,结合聚合关系、对文形式、具体语境等进行言语推义,这一诗句中的“骤”应为“屡次”义。

关键词： “骤” “屡次” “急遽”

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