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Silencing of Bcl-2 gene expression by siRNA transfection in- hibits the protective effect of fluvastatin against cell apoptosis in human aortic endothelial cells

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Journal of Geriatric Cardiology 2008年第1期5卷 33-38页

作者： wenwen zhong yang liu Jian Li hui tian Department of Geriatric Endocrinology Chinese PLA General HospitalBeijing 100853China Department of Geriatric EndocrinologyChinese PLA General HospitalBeijing 100853China Department of Geriatric EndocrinologyChinese PLA General HospitalBeijing 100853China Department of Geriatric EndocrinologyChinese PLA General HospitalBeijing 100853China

Objective To study the protective effect of fluvastatin,one of the HMG-CoA reductase inhibitors (statins),against oxygen radical-induced oxidative damages in human aortic endothelial cell,and the role of Bcl-2 in this *** Human aortic endothelial cells with or without Bcl-2 siRNA transfection were subjected to 1-100 nM of fluvastatin and 100 la hydrogen peroxide for 24 ***-2 mRNA and protein expression were measured by Taqman quantitative PCR and Western *** apoptosis was measured by normal and fluorescent microscopy and Cell Death Detection *** In the Bcl-2-expressed cells,fluvastatin significantly reversed hydrogen peroxide-induced microscopic apoptosis and apoptotic DNA fragmentation,which were accompanied by a markedly upregulation of Bcl-2 expression by ***,the endothelial protection by fluvastatin was completely lost in Bcl-2 siRNA transfected *** Fluvastatin protects human endothelial cells against oxygen radical-induced cell apoptosis in vitro,and this protection seemed to be mediated in a Bcl-2 dependent pathway.(J Geriatr Cardil 12008;5:33-38)

关键词： fluvastatin,Bcl-2,gene silencing,apoptosis

Isoflavone genistein protects high glucose-induced human aortic endothelial cell apoptosis through estrogen receptor-mediated pathway

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Journal of Geriatric Cardiology 2008年第2期5卷 86-90页

作者： wenwen zhong yang liu Guang yang hui tian Department of Geriatric Endocrinology Chinese PLA General Hospital Beijing100853China Department of Geriatric EndocrinologyChinese PLA General Hospital Beijing100853China Department of Geriatric EndocrinologyChinese PLA General Hospital Beijing100853China Department of Geriatric EndocrinologyChinese PLA General Hospital Beijing100853China

Objective The aim of this study was to determine if isoflavone genistien has protective effects against high glucose-induced cell apoptosis in human aortic endlthelial cells, and investigate the possible mechanism for this protection. Methods Human aortic endothelial cells subjected to normal (5mmol/L) or high glucose (25mmol/L) were treated with genistein at 0, 50, 100nmol/L. Parallel experiments were performed with 100nM 17b-estradiol, and also in the presence and absence of the pure anti-estrogen ICI-182,780 (100nmol/L). The effects on cell apoptotic DNA fragmentation were determined using cell death ELISA, and the effects on cellular proliferation were determined using tritiated thymidine incorporation assay. Estrogen receptor expression was detected by Taqman quantitative PCR. Results Genistein at 100nmol/L significantly reduced high glucose-induced DNA fragmentation, and reversed cell DNA synthesis inhibition (P <0.001) after 24 hours' incubation. The effect of genistein was completely blocked by ICI-182,780 administration. Estrogen receptor beta, but not alpha was found to be expressed in these cells. Conclusion Isoflavone genistein shows protection against high glucose-induced cell damage through estrogen receptor beta, reducing apoptotic DNA damage and protecting from the inhibition of cell proliferation.

关键词： phytoestrogen endothelial cell high glucose estrogen receptor apoptosis

Exploring the characteristics of tourism industry by analyzing consumer review contents from social media:a case study of Bamako,Mali

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Geo-Spatial Information Science 2019年第3期22卷 214-222页

作者： Sanogo Bruno Chao yang wenwen tian zhong Xie Yuanzheng Shao School of Geography and Information Engineering China University of GeosciencesWuhanHubeiChina Agence pour le Promotion Touristique du Mali(APTM) HamdallayeBamakoMali Collaborative Innovation Center of Geospatial Technology Wuhan UniversityWuhanChina

In this Web 2.0 era,various and massive tourist experiences and reviews presented on social networks have become important information for tourism *** this paper,we apply social media to explore and study the tourism industry of Bamako,*** 2000 reviewers and their comments about Bamako’s hotels and restaurants from TripAdvisor and Facebook were ***,we integrate official tourism statistic data and field surveying data into the online review *** mining and statistic method are used to analyze the data for purpose of exploring the characteristics about tourism industry in *** we find that:(i)Most tourists are coming to Bamako for business purpose,and they incline to choose the hotels with better service and security condition;(ii)Comments on social media would greatly affect travelers’choice on hotels;(iii)Most travelers are satisfied about Bamako’s accommodation services.

关键词： Social media online reviews tourism industry study Bamako Mali

Aluminum and phosphorus codoped “superaerophobic” Co3O4 microspheres for highly efficient electrochemical water splitting and Zn-air batteries

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Journal of Energy Chemistry 2020年第11期29卷 324-331页

作者： Xian-Wei Lv Yuping liu wenwen tian Lijiao Gao zhong-Yong Yuan National Institute for Advanced Materials School of Materials Science and EngineeringNankai UniversityTianjin 300350China Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) College of ChemistryNankai UniversityTianjin 300071China

Multifunctional non-precious catalysts for hydrogen/oxygen evolution reaction(HER/OER) and oxygen reduction reaction(ORR) constitute the bottleneck in the applications in electrochemical overall water splitting(OWS) and Zn-air batteries. Herein, a trifunctional electrocatalyst of urchin-like Al,P-codoped Co3O4 microspheres supported on Ni foam(denoted as AP-CONPs/NF) was fabricated via a hydrothermal process and subsequent low-temperature annealing and phosphorization, exhibiting enhanced OER, HER and ORR activities compared with single-doped and undoped samples. Their surface self-organized microstructure and excellent "superaerophobic" feature make a high bubble repellency, which boost diffusion of reactants and electrolyte-electrode intimate contact. The codoping of Al and P elements into Co3O4 betters right the balance among surface chemical state, the increased oxygen vacancies and the promoted charge transfer. Encouraged by these synergistic advantages, the AP-CONPs/NF was further employed as excellent bifunctional electrodes for the OWS(low cell voltage of 1.57 V at 10 mA cm-2) and as air cathode for rechargeable Zn-air batteries(high power density of 89.1 mW cm-2), which demonstrates a great feasibility for practical applications.

关键词： Trifunctional electrocatalysts Co3O4 Overall water splitting Zn-air batteries "Superaerophobic"surface Anion-cation double doping

Core-shell gold-copper nanoparticles:Evolution of copper shells on gold cores at different gold/copper precursor ratios

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Chinese Journal of Structural Chemistry 2023年第11期42卷 32-39页

作者： Shaonan tian Yu Zhang Qing Zeng Junyu zhong hui liu Lin Xu Jun yang State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing100190China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China State Key Laboratory of Bio-fibers and Eco-textiles Qingdao UniversityQingdao266071China School of Chemistry and Materials Science Jiangsu Key Laboratory of New Power BatteriesJiangsu Collaborative Innovation Centre of Biomedical Functional MaterialsNanjing Normal UniversityNanjing210023China

Core-shell nanostructures usually exhibit tunable catalytic properties in comparison with their single core or shell counterpart due to electronic interaction and lattice strain between the core and shell ***,we report the intriguing evolution of copper(Cu)shells on the gold(Au)cores at different Au/Cu precursor ratios during the synthesis of core-shell Au-Cu nanoparticles at an organic medium via seed-mediated growth *** brief,at relatively low Cu ratios,quasi-spherical Au-Cu nanoparticles with conventional core-shell structures are the majority products,in which the Cu shell thickness increases with the increase of Cu precursor *** dif-ference is that at high Cu ratios,the Cu shells no longer increase their thickness,but evolve into a dendritic ***,the core-shell Au-Cu nanoparticles with dendritic Cu shells could be transformed into interesting Au-Cu cage-bell structures after a ripening process at elevated ***,through galvanic replacement reaction with Pt precursors,the thin Cu shells could be converted into CuPt alloy shells on the Au cores,which exhibit enhanced activity towards methanol oxidation reaction with satisfactory durability,in comparison with that of commercial Pt/C catalysts.

关键词： Core-shell nanostructure Seed-mediated growth Electronic interaction Lattice strain Methanol oxidation reaction

A 7-year follow-up study of the features and transformations of elderly male patients with OGTT-1h hyperglycemia

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Frontiers of Medicine 2008年第4期2卷 396-399页

作者： hui tian Changyu PAN Juming LU Chunlin LI wenwen zhong Xiutang CAO Department of Elderly Endocrinology General Hospital of PLABeijing 100853China Department of Endocrinology General Hospital of PLABeijing 100853China Department of Medical Management General Hospital of PLABeijing 100853China

The aim of this paper is to investigate the clin-ical features and transformation of elderly male patients with normal blood glucose levels at fasting and 2 hours after glucose intake but with hyperglycemia(>11.1 mmol/L)1 hour after oral glucose tolerance test(OGTT-1h HG).Seven years of follow-up visits were per-formed on 189 elderly male outpatients with OGTT-1h HG and data was recorded on their body mass index(BMI),blood pressure,serum cholesterol and triglyceride test results and on their glucose tolerance changes every 1–2 years after taking OGTT;their possible causes were ***-up visits revealed that 19 patients with OGTT-1h HG were diagnosed with diabetes(10.1%),78 patients with impaired glucose tolerance(IGT,41.3%),2 patients transformed to normal glucose tolerance(NGT,1.1%)and the remaining 90 patients(47.6%)remained *** comparison with IGT patients showed that the ratio of OGTT-1h HG patients turning to diabetes was lower than that of IGT patients(21.1%,x2513.05,P,0.01),and the ratio of OGTT-1h HG patients transforming to NGT was slightly higher(0.4%,x252.46,P.0.05).The prevalence of complications of hypertension,coronary heart disease,cerebral vascular diseases and dyslipidemia in patients with OGTT-1h HG were higher than those with NGT(P,0.05)and were similar to that of IGT *** a special phenotype of OGTT and as part of an abnormal glucose tolerance con-formation,patients with OGTT-1h HG warrant special attention,since about half of them were found to have developed diabetes or IGT,and their risk of suffering from vascular diseases were also increased.

关键词： glucose tolerance test clinical features trans-formation

Anodal transcranial direct current stimulation alleviates cognitive impairment in an APP/PS1 model of Alzheimer’s disease in the preclinical stage

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Neural Regeneration Research 2022年第10期17卷 2278-2285页

作者： Yin-Pei Luo Zhi liu Cong Wang Xiu-Fang yang Xiao-Ying Wu Xue-Long tian hui-zhong Wen Chongqing Key Laboratory of Neurobiology Department of NeurobiologySchool of Basic MedicineArmy Medical UniversityChongqingChina Chongqing Medical Electronics Engineering Technology Research Center Laboratory of Neural Regulation and Rehabilitation TechnologyCollege of BioengineeringChongqing UniversityChongqingChina Department of Histology and Embryology School of Basic MedicineArmy Medical UniversityChongqingChina

Anodal transcranial direct current stimulation(AtDCS)has been shown to alleviate cognitive impairment in an APP/PS1 model of Alzheimer’s disease in the preclinical ***,this enhancement was only observed immediately after AtDCS,and the long-term effect of AtDCS remains *** this study,we treated 26-week-old mouse models of Alzheimer’s disease in the preclinical stage with 10 AtDCS sessions or sham *** Morris water maze,novel object recognition task,and novel object location test were implemented to evaluate spatial learning memory and recognition memory of *** blotting was used to detect the relevant protein *** changes were observed using immunohistochemistry and immunofluorescence *** weeks after treatment,the mice subjected to AtDCS sessions had a shorter escape latency,a shorter path length,more platform area crossings,and spent more time in the target quadrant than sham-stimulated *** mice subjected to AtDCS sessions also performed better in the novel object recognition and novel object location tests than sham-stimulated ***,AtDCS reduced the levels of amyloid-β42 and glial fibrillary acidic protein,a marker of astrocyte activation,and increased the level of neuronal marker NeuN in hippocampal *** findings suggest that AtDCS can improve the spatial learning and memory abilities and pathological state of an APP/PS1 mouse model of Alzheimer’s disease in the preclinical stage,with improvements that last for at least 6 weeks.

关键词： Alzheimer’s disease amyloid-β anodal transcranial direct current stimulation cognitive function inflammation long-term effect neuron preclinical stage

Molecular core–shell structure design:Facilitating delayed fluorescence in aggregates toward highly efficient solution-processed OLEDs

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Aggregate

Aggregate 2022年第2期3卷 132-142页

作者： Dan liu Meng Zhang wenwen tian Wei Jiang Yueming Sun Zheng Zhao Ben zhong Tang Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and DeviceSchool of Chemistry and Chemical EngineeringSoutheast UniversityNanjingP.R.China Shenzhen Institute of Aggregate Science and Technology School of Science and EngineeringThe Chinese University of Hong KongGuangdongChina

Light has been sought and explored by human since ancient *** the most important form of light,fluorescence is significant to applications in bioimaging and optoelectronic ***,fluorescence quenching problem constitutes a serious bottleneck in materials *** from the core–shell structure in nature,we report an effective strategy to overcome this long-standing problem by utilizing a molecular core–shell *** an emissive core and multifunctional shell fragments,these compounds show aggregation-induced delayed fluorescence(AIDF)properties by restricting singlet oxygen(^(1)O_(2))generation and suppressing the triplet–triplet annihilation(TTA).Protected by the functional shell,the aggregation-induced emission luminogens(AIEgens)exhibit strong emission with high photoluminescent quantum yield and exciton ***,because the shell materials can form exciplex with electron-transport materials,the fully solution-processed organic light-emitting diodes(OLEDs)based on these core–shell materials show low turnon voltages,excellent device performance with current efficiency of 61.4 cd A–1 and power efficiency of 42.8 lm W–1,which is a record-breaking efficiency based on all-solution processed organic multilayer systems among the AIE-OLEDs so *** simple visualization strategy based on molecular core–shell structure provides a promising platform for AIEgens used in the fully wet-processed optoelectronic field.

关键词： aggregation-induced emission(AIE) molecular core-shell structure organic light-emitting diodes(OLEDs) triplet-triplet annihilation(TTA)

Hybrid Modeling for Soft Sensing of Molten Steel Temperature in LF

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Journal of Iron and Steel Research International 2009年第4期16卷 1-6页

作者： tian hui-xin MAO Zhi-zhong WANG An-na School of Electrical and Automation Engineering Tianjin Polytechnic University Tianjin 300160 China School of Information Science and Engineering Northeastern University Shenyang 110004 Liaoning China Key Laboratory of Integrated Automation of Process Industry of Ministry of Education NortheasternUniversity Shenyang 110004 Liaoning China

Aiming at the limitations of traditional thermal model and intelligent model, a new hybrid model is established for soft sensing of the molten steel temperature in LF. Firstly, a thermal model based on energy conservation is described; and then, an improved intelligent model based on process data is presented by ensemble ELM （extreme learning machine） for predicting the molten steel temperature in LF. Secondly, the self-adaptive data fusion is pro- posed as a hybrid modeling method to combine the thermal model with the intelligent model. The new hybrid model could complement mutual advantage of two models by combination. It can overcome the shortcoming of parameters obtained on-line hardly in a thermal model and the disadvantage of lacking the analysis of ladle furnace metallurgical process in an intelligent model. The new hybrid model is applied to a 300 t LF in Baoshan Iron and Steel Co Ltd for predicting the molten steel temperature. The experiments demonstrate that the hybrid model has good generalization performance and high accuracy.

关键词： ladle furnace hybrid modeling soft sensing thermal model data fusion