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Magnetic properties and crystallization kinetics of Zn_(0.5) Ni_(0.5) Fe_2O_4

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Rare Metals 2011年第6期30卷 621-626页

作者： WU wenwei cai Jinchao WU Xuehang LI Yongni LIAO Sen School of Chemistry and Chemical Engineering Guangxi University Nanning 530004 P.R. China

Precursor of nanocrystalline ***2O4 was obtained by grinding mixture of ZnSO4.7H2O, NiSO4.6H2O, FeSO4.7H2O, and Na2CO3.10H2O under the condition of suffactant polyethylene glycol （PEG）-400 being present at room temperature, washing the mixture with water to remove soluble inorganic salts and drying it at 373 K. The spinel Zn0.5Ni0.5Fe2O4 was obtained via calcining precursor above 773 K. The precursor and its calcined products were characterized by differential scanning calorimetry （DSC）, Fourier transform infrared （FF-IR）, X-ray diffraction （XRD）, and vibrating sample magnetometer （VSM）. The result showed that ***204 obtained at 1073 K had a saturation magnetization of 74 ***-1. Kinetics of the crystallization process of Zn0.5Ni0.5Fe2O4 was studied using DSC technique, and kinetic parameters were determined by Kissinger equation and Moynihan et al. equation. The value of the activation energy associated with the crystallization process of Zr0.5Ni0.5Fe2O4 is 220.89 kJ-mol-1. The average value of the Avrami exponent, n, is equal to 1.59±0.13, which suggests that crystallization process of Zn0.5Ni0.5Fe2O4 is the random nucleation and growth of nuclei reaction.

关键词： magnetic material Zn0.5Ni0.5Fe2O4 non-isothermal kinetics crystallization process solid-state reaction at low heat

Comprehensive Evaluation of Aluminum Tolerance in Sugarcane Varieties at the Seedling Stage and Screening of Identification Indexes

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Agricultural Biotechnology 2022年第6期11卷 64-70页

作者： Lishun PENG Zhengying CAO Benpeng YANG wenwei cai Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural SciencesHaikou 571101China

[Objectives]Aluminum toxicity is one of the important stress factors affecting sugarcane quality and yield in south China,breeding aluminum tolerant varieties is a cost-effective and practical *** order to explore the characteristics of aluminum toxicity tolerance in different sugarcane varieties and establish the comprehensive evaluation system for screening and breeding new varieties with aluminum toxicity tolerance,this study was conducted to perform comprehensive evaluation of aluminum tolerance in different sugarcane varieties and screening of identification indexes.[Methods]With 26 sugarcane varieties bred in different ecological areas as materials,through a pot experiment,nine indexes which include sugarcane plant height,root length,shoot dry weight,root dry weight,root-shoot ratio,relative chlorophyll content,leaf proline content,and shoot and root aluminum contents at the seedling stage under acid-aluminum stress and control conditions were measured,and the aluminum toxicity tolerance coefficients of various indexes of each variety were *** this basis,the aluminum tolerance of all varieties was comprehensively evaluated by principal component analysis,subordinate function,cluster analysis and stepwise regression analysis.[Results]Compared with the control,the nine indexes showed more abundant variation among the 26 varieties under acid-aluminum *** correlation analysis confirmed that the contributions of the nine measured indexes to aluminum tolerance were significantly overlapped,and the subsequent principal component analysis summarized and combined the ones with strong correlation among the nine indexes,converting them into two new independent comprehensive *** cumulative contribution rate showed that the two new indexes covered 82.448%of the information of the original nine ***,the comprehensive evaluation D values of the acid-aluminum tolerance of the 26 varieties were calculated based on the subordinate function va

关键词： Sugarcane Acid-aluminum tolerance Principal component analysis Subordinate function Comprehensive evaluation

Comparative Preliminary Evaluation of Agronomic and Quality Traits of New"Zhongtang"Superior Lines

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Asian Agricultural Research 2024年第6期16卷 48-54页

作者： Zhengying CAO Lishun PENG Yimei GAN wenwei cai Yuanli WU Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural SciencesHaikou 571101China Sanya Research Institute Chinese Academy of Tropical Agricultural SciencesSanya 572024China

[Objectives]Sugarcane is the most significant sugar cash crop in the tropical and subtropical regions of ***,it is notable for its narrow genetic basis,limited trait improvement,weak adaptability of varieties,and poor planting *** order to accelerate the process of sugarcane variety replacement and expand the basis of genetic variation,interspecific hybridization and multiple mutagenesis are the most effective methods for obtaining new sugarcane *** evaluation and identification of phenotypic traits of germplasm represents a significant analytical ***"Zhongtang"series of sugarcane varieties is distinguished by its high yield and high sugar *** sugarcane germplasms with excellent agronomic and quality traits can be identified and developed as breeding parents and new breeding lines through comprehensive evaluation of the existing germplasm.[Methods]A total of 181 new lines were selected through genetic origin and orientation,and evaluated and appraised for growth,yield,and economic *** data on 6 important agronomic and quality traits,including tillering,initial plant height,plant height,stem diameter,number of effective stems,and brix at maturity,were used to discover superior *** traits were evaluated during the two production seasons of the participant lines.[Results]A comprehensive evaluation of seedling growth traits and economic traits at maturity of the experimental lines identified 30 excellent new germplasms of *** the selected lines,1501 and 1701 exhibited superior agronomic and quality traits,rendering them suitable as parental lines for sugarcane breeding or breeding as new varieties.[Conclusions]The exemplary results obtained in this study provide a solid foundation for the improvement of sugarcane germplasm,with the goal of enhancing quality and *** findings are of great scientific and practical significance to the study of sugarcane yield and sugar-related gene

关键词： Sugarcane Germplasm evaluation Yield Agronomic trait Economic trait

Cloning and Analysis of TasA Gene in Bacillus subtilis HAS

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Agricultural Biotechnology 2017年第2期6卷 48-50,54页

作者： Guoruo XIONG Gengfeng ZHAO wenwei cai Suran WU Benpeng YANG Shuzhen ZHANG Institute of Tropical Biotechnology/Sugarcane Research Center Chinese Academy of Tropical Agricultural Sciences Department of Biochemistry Hainan Medical College

In order to clarify the mechanism for the inhibitory action of the Bacillus subtilis strain HAS on Sporisorium scitamineum （ Syd. ）, which can cause sugarcane smut, the full-length TasA gene which encodes a protein with broad-spectrum antimicrobial activity, was PCR-amplified from HAS, and cloned into pMD18-T vector. Sequence analysis indicated that the full-length TasA cloned from HAS consisted of 786 nucleotides, and shared 99% homology in nucleotide sequence with the TasA gene sequence published in Genbank （AJ871386.1）. It was predicted that the molecular weight of TasA protein was about 28 kD. Base transitions or transversions ~curred at positions 104, 164, 169,250, 399,623 and 627, at the 2nd, 2nd, 3rd , 1st , 3rd, 2nd and 3rd bases of TasA codons. The mutations in the seven bases may cause the missense mutations of the polypeptide chain. Compared with the amino acid sequences of the TasA protein encoded by Bacillus aubtilis subsp, subtilis str. 168, mutations in two amino acids at positions 150 and 209 of the protein encoded by the cloned TasA gene were found, and as a result, an ala- nine was replaced with a threonine.

关键词： Bacillus subtilis HAS mad a glutamic acid was replaced with an asparagine. Sporisorium scitamineum ( Syd. ) Antimicrobial protein TasA

Resistance Evaluation of 23 Sugarcane Varieties to Smut

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Plant Diseases and Pests 2017年第3期8卷 29-31页

作者： Xiong Guoru cai wenwei Wang Wenzhi Wu Suran Yang Benpeng Zhang Shuzhen Institute of Tropical Bioscience and Biotechnology/Sugarcane Research Center CATASKey Laboratory of Biology and Genetic Resources of Tropical Cropsthe Ministry of Agriculture

[ Objective] The paper aimed to screen sugarcane varieties and species layout suitable for Hainan sugarcane area. [ Method] Using mixed spores of Ustilago scitaminea Sydow, smut resistance of 21 major sugarcane varieties in national sugarcane regional trial as well as ROC22 and ROC16 were identified through artificial inoculation test and field natural incidence. [ Result] Yunzhe 99-596, Yuegan 42, Guitang 29 and Funong 39 reached highly resistant and resistant levels to smut in artificial inoculation test. [ Conclusion] Most of the varieties showed better resistance to smut in newly planted sugarcane and ratoon sugarcane except Yunzhe 03-103, Liucheng 03-1137 and ROC22.

关键词： Sugarcane Ustilago scitaminea Sydow Resistance identification

Cloning and Analysis of Chitinase Gene in Bacillus subtilis HAS

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Plant Diseases and Pests 2019年第2期10卷 4-6页

作者： Xiong Guoru Zhao Gengfeng cai wenwei Wang Jungang Yang Benpeng Zhang Shuzhen Institute of Tropical Biotechnology/Sugarcane Research Center CATAS Department of Biochemistry Hainan Medical College

[Objective] The paper was to study the possible mechanism of chitinase gene. [Method] The chitinase gene with inhibitory effect against a variety of pathogenic fungi was cloned and analyzed in this study. [Result] The genome of HAS strain contained an ORF of 834 bp. The homology of coding protein corresponding to ORF with chitosanase [Bacillus subtilis subsp. subtilis str. 168](ref|NP_390566.1) was the highest of 98%(272/277).[Conclusion]Through the analysis of amino acid sequence and secondary structure of the protease, tertiary structure prediction and analysis, HAS strain may play a pivotal role in controlling Sporisorium scitaminea Syd.

关键词： Bacillus subtilis HAS Sporisorium scitaminea Syd. Antifungal protein Chitinase gene

Screening and Identification of Antagonistic Strain HAS against Sporisorium scitaminea Sydow

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Plant Diseases and Pests 2014年第3期5卷 20-23页

作者： Xiong Guoru Zhao Gengfeng Wu Suran Feng Cuilian cai wenwei Zhang Shuzhen Institute of Tropical Biotechnology /Sugarcane Research Center CATAS Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of Agriculture

The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showed that 16 S rDNA gene fragment with the size of 1 533 bp was obtained,and its sequence had the homology of 99% with the sequence of Bacillus subtilis. The morphological characteristics of the strain were as follows： the mycelium of strain HAS was short-rod shape,and could move; Gram staining was positive; 3% KOH solubility test was negative; flagellum was grown around the cells; spores were grown in the middle,elliptic,and cysts were slightly swelled. The strain was preliminarily identified as B. subtilis. Dual culture with pathogenic fungi showed that the strain had good inhibitory effect against various pathogenic fungi causing diseases on sugarcane and other crops. Therefore,strain HAS isolated in the study was identified to be ***,which had good control effect against S. scitaminea.

关键词： Bacillus subtilis HAS Sporisorium scitaminea Sydow Biological control

Motor vehicle crash patients:An international comparison China *** US

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Motor vehicle crash patients:An international comparison Chi...

Effects of Space Radiation Mutation on Germination and Growth of Sugarcane Hybrid Seeds

中华医学会急诊医学分会第十六次全国急诊医学学术年会

作者： Jianfeng Tu wenwei cai Xianghong Yang Yue Yang Ko Paul Department of Emergency Medcine Zhejiang Provincial People’s Hospital Zhejiang Medical College Department of Emergency Medicine SUNY Upstate Medical University

Objectives To identify similarities and differences in ED patients involved in motor vehicle incidents in China and jn theUS. Methods Zheijiang Provincial People’s Hospital,one of the largest public hospitals in Hangzhou,China,is designated a grade 3(highest level in China)hospital, with 130.000 annual ED *** Upstate Medical

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Agricultural Biotechnology 2019年第6期8卷 15-18页

作者： Yimei GAN Yuanli WU Jun ZENG wenwei cai Zhengying CAO Lishun PENG Benpeng YANG Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of AgricultureSugarcane Research Center of Chinese Academy of Tropical Agricultural SciencesInstitute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences

[Objectives] This study was conducted to investigate the mutagenic effects of space radiation mutagenesis on sugarcane hybrid seeds. [Methods] Sugarcane hybrid spikes were subjected to space radiation mutagenesis treatment to identify the effects on the germination, seedling growth and plant growth and development of sugarcane hybrid seeds. [Results] The space radiation treatment had a great impact on the germination and growth of sugarcane hybrid seeds, especially on the germination of sugarcane seeds. The germination number per gram of seeds increased significantly, and the survival rate of seedlings increased significantly. The effect on the growth status was relatively small. The average number of tillers decreased slightly, the percentage of available stems increased, and the plants became higher, which resulted in a greater effect on sugarcane yield. Sugarcane sucrose was increased. The seed germination and late growth status indicated that space radiation promoted the germination and growth of sugarcane hybrid seeds. [Conclusions] This study provides a basis for the space radiation mutagenesis breeding of sugarcane hybrid seeds and the promotion of sugarcane breeding process.

关键词： Space radiation Sugarcane Mutation breeding Seed germination