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Effects of drip and flood irrigation on carbon dioxide exchange and crop growth in the maize ecosystem in the Hetao Irrigation District,China

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Journal of Arid Land 2024年第2期16卷 282-297页

作者： LI Chaoqun han wenting PENG Manman College of Mechanical and Electrical Engineering Heze UniversityHeze 274015China College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100China Institute of Soil and Water Conservation Northwest A&F UniversityYangling 712100China

Drip irrigation and flood irrigation are major irrigation methods for maize crops in the Hetao Irrigation District,Inner Mongolia Autonomous Region,China.This research delves into the effects of these irrigation methods on carbon dioxide(CO_(2))exchange and crop growth in this region.The experimental site was divided into drip and flood irrigation zones.The irrigation schedules of this study aligned with the local commonly used irrigation schedule.We employed a developed chamber system to measure the diurnal CO_(2)exchange of maize plants during various growth stages under both drip and flood irrigation methods.From May to September in 2020 and 2021,two sets of repeated experiments were conducted.In each experiment,a total of nine measurements of CO_(2)exchange were performed to obtain carbon exchange data at different growth stages of maize crop.During each CO_(2)exchange measurement event,CO_(2)flux data were collected every two hours over a day-long period to capture the diurnal variations in CO_(2)exchange.During each CO_(2)exchange measurement event,the biological parameters(aboveground biomass and crop growth rate)of maize and environmental parameters(including air humidity,air temperature,precipitation,soil water content,and photosynthetically active radiation)were measured.The results indicated a V-shaped trend in net ecosystem CO_(2)exchange in daytime,reducing slowly at night,while the net assimilation rate(net primary productivity)exhibited a contrasting trend.Notably,compared with flood irrigation,drip irrigation demonstrated significantly higher average daily soil CO_(2)emission and greater average daily CO_(2)absorption by maize plants.Consequently,within the maize ecosystem,drip irrigation appeared more conducive to absorbing atmospheric CO_(2).Furthermore,drip irrigation demonstrated a faster crop growth rate and increased aboveground biomass compared with flood irrigation.A strong linear relationship existed between leaf area index and light utilization efficiency,irrespective of the irrigation m

关键词： carbon dioxide exchange maize growth drip irrigation harvest index net primary productivity Hetao Irrigation District

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Antitumor effects of a novel photosensitizer-mediated photodynamic therapy and its influence on the cell transcriptome

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Oncology Research 2024年第5期32卷 911-923页

作者： JINGJING CHEN DAN WANG ZEQUN WANG MENGYUAN han HOUQING YIN wenting ZHOU RIBAI YAN YAN PAN Department of Pharmacology School of PharmacyChangzhi Medical CollegeChangzhiChina2 Department of PharmacologySchool of Basic Medical SciencesHealth Science CenterPeking UniversityBeijingChina3 Beijing Key Laboratory of Tumor Systems BiologyPeking UniversityBeijingChina4 Department of PharmacologyXinjiang Medical UniversityUrumqiChina5 School of Pharmaceutical SciencesPeking UniversityBeijingChina

Photodynamic therapy(PDT)is a promising cancer treatment.This study investigated the antitumor effects and mechanisms of a novel photosensitizer meso-5-[ρ-diethylene triamine pentaacetic acid-aminophenyl]−10,15,20-triphenyl-porphyrin(DTP)mediated PDT(DTP-PDT).Cell viability,reactive oxygen species(ROS),and apoptosis were measured with a Cell Counting Kit-8 assay,DCFH-DA fluorescent probe,and Hoechst staining,respectively.Cell apoptosis-and autophagy-related proteins were examined using western blotting.RNA sequencing was used to screen differentially expressed mRNAs(DERs),and bioinformatic analysis was performed to identify the major biological events after DTP-PDT.Our results show that DTP-PDT inhibited cell growth and induced ROS generation in MCF-7 and SGC7901 cells.The ROS scavenger N-acetyl-L-cysteine(NAC)and the P38 MAPK inhibitor SB203580 alleviated DTP-PDT-induced cytotoxicity.DTP-PDT induced cell apoptosis together with upregulated Bax and downregulated Bcl-2,which could also be inhibited by NAC or SB203580.The level of LC3B-Ⅱ,a marker of autophagy,was increased by DTP-PDT.A total of 3496 DERs were obtained after DTP-PDT.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that DERs included those involved in cytosolic ribosomes,the nuclear lumen,protein binding,cell cycle,protein targeting to the endoplasmic reticulum,and ribosomal DNA replication.Disease Ontology and Reactome enrichment analyses indicated that DERs were associated with a variety of cancers and cell cycle checkpoints.Protein-protein interaction results demonstrated that cdk1 and rps27a ranked in the top 10 interacting genes.Therefore,DTP-PDT could inhibit cell growth and induce cell apoptosis and autophagy,partly through ROS and the P38 MAPK signaling pathway.Genes associated with the cell cycle,ribosomes,DNA replication,and protein binding may be the key changes in DTP-PDT-mediated cytotoxicity.

关键词： Photodynamic therapy ROS Apoptosis Autophagy Bioinformatic analysis

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Lignocellulosic fraction-induced niche differentiation within dissimilatory iron reducing bacterial groups in a paddy soil

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Soil Ecology Letters 2024年第1期6卷 77-88页

作者： Yunbin Jiang wenting Hu Kailou Liu Shangshu Huang Fengwu Zhou Cheng han Huan Deng Wenhui Zhong Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control School of GeographyNanjing Normal UniversityNanjing 210023China Jiangsu Centerfor Collaborative Innovation in Geographical Information Resource Development and Application Nanjing 210023China National Engineering and Technology Research Center for Red Soil Improvement Jiangxi Institute of Red Soil and Germplasm ResourcesNanchang 331717China Schoolof Environment Nanjing Normal UniversityNanjing 210023China

Responses of soil DIRB to lignocellulosic fractions during a 6-week microcosm incubation were investigated.Anaeromyxobacter,Bacillus,and Clostridium maintained their domi-nance throughout the incubation.Distinct DIRB groups proliferated under specific lignocellulosic fraction amendments.Limits of insufficient ferric iron on the proliferation varied by DIRB group.Dissimilatory iron reducing bacteria(DIRB)are phylogenetically and physi-ologically diverse in paddy soils,where iron reduction closely couples with the oxidation of rice straw-derived carbon in the straw returning scenarios.However,few studies have addressed the niche differentiation within DIRB groups during the degradation of lignocellulosic fractions of rice straw.This study conducted a 6-week microcosm incubation experiment to reveal the distinct responses of DIRB groups under specific lignocellulosic fraction amendments with and without ferrihydrite(Fh)addition in a flooded paddy Ultisol.Results showed that the total absolute abundance of the 19 detected DIRB groups did not vary significantly during the incubation.Anaeromyxobacter,Bacillus,and Clostridium were the dominant DIRB groups for all lignocellulosic treatments whereas Thermincola was dominant but only under xylan amendment with Fh addition.DIRB-nodes in the co-occurrence networks of bacterial community mainly belonged to Anaeromyxobacter and Bacillus.Clostridium and Thermincola,Alkaliphilus and Anaeromyxobacter,and Alicyclobacillus,Desulfobulbus,and Desulfosporosinus were specifically proliferated under xylan,cellulose,and lignin amendments,respectively.Whether the proliferation was limited by insufficient ferric iron varied by bacterial group.These findings suggested the lignocellulosic fraction-induced niche differentiation within DIRB groups,which advanced our understanding of the ecology of DIRB in paddy soils under straw returning.

关键词： cellulose hemicellulose lignin bacterial community diversity co-occurrence networks

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Estimation of transpiration coefficient and aboveground biomass in maize using time-series UAV multispectral imagery

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The Crop Journal 2022年第5期10卷 1376-1385页

作者： Guomin Shao wenting han Huihui Zhang Yi Wang Liyuan Zhang Yaxiao Niu Yu Zhang Pei Cao College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100ShaanxiChina Key Laboratory of Agricultural Internet of Things Ministry of AgricultureYangling 712100ShaanxiChina Institute of Water-Saving Agriculture in Arid Areas of China Northwest A&F UniversityYangling 712100ShaanxiChina Water Management and Systems Research Unit USDA-ARS2150 Centre AvenueBldg.D.Fort CollinsCO 80526USA College of Information Xi’an University of Finance and EconomicsXi’an 710100ShaanxiChina Institute of Soil and Water Conservation Northwest A&F UniversityYangling 712100ShaanxiChina University of Chinese Academy of Sciences Beijing 100049China

Estimating spatial variation in crop transpiration coefficients(CTc) and aboveground biomass(AGB)rapidly and accurately by remote sensing can facilitate precision irrigation management in semiarid regions. This study developed and assessed a novel machine learning(ML) method for estimating CTc and AGB using time-series unmanned aerial vehicle(UAV)-based multispectral vegetation indices(VIs)of maize under several irrigation treatments at the field scale. Four ML regression methods: multiple linear regression(MLR), support vector regression(SVR), random forest regression(RFR), and adaptive boosting regression(ABR), were used to address the complex relationship between CTcand VIs. AGB was then estimated using exponential, logistic, sigmoid, and linear equations because of their clear mathematical formulations based on the optimal CTcestimation model. The UAV VIs-derived CTcusing the RFR estimation model yielded the highest accuracy(R^(2)= 0.91, RMSE = 0.0526, and n RMSE = 9.07%). The normalized difference red-edge index, transformed chlorophyll absorption in reflectance index, and simple ratio contributed significantly to the RFR-based CTcmodel. The accuracy of AGB estimation using nonlinear methods was higher than that using the linear method. The exponential method yielded the highest accuracy(R^(2)= 0.76, RMSE = 282.8 g m, and n RMSE = 39.24%) in both the 2018 and 2019 growing seasons. The study confirms that AGB estimation models based on cumulative CTcperformed well under several irrigation treatments using high-resolution time-series UAV multispectral VIs and can support irrigation management with high spatial precision at a field scale.

关键词： Crop transpiration Normalized difference red-edge index Unmanned aerial vehicles Random forest regression Biomass

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基于礼物交换博弈实验的互惠机制对医生利他行为激励的启示

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中国卫生经济 2024年第1期43卷 1-3页

作者：刘文婷韩优莉首都医科大学公共卫生学院北京100069

目的:从维护利他性角度为构建符合医疗行业特点的激励制度提供思路。方法:将经济学领域的互惠利他理论和礼物交换博弈实验引入医生利他行为激励。结果:提出医疗领域医、患、管三方委托代理关系的间接互惠利他理论和注重健康价值衡量的... 详细信息

目的:从维护利他性角度为构建符合医疗行业特点的激励制度提供思路。方法:将经济学领域的互惠利他理论和礼物交换博弈实验引入医生利他行为激励。结果:提出医疗领域医、患、管三方委托代理关系的间接互惠利他理论和注重健康价值衡量的薪酬激励设想,构建基于三方礼物交换博弈实验的医生利他行为激励机制模型。结论:采用价值医疗理念对医生利他行为进行激励,构建符合医疗行业特点的激励制度,可促进医疗领域高质量发展。

关键词：互惠利他礼物交换医生激励

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设施生菜光合和蒸腾速率影响因素分析与预测模型构建

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农业机械学报 2024年第1期55卷 339-349页

作者：张增林杨杰郭常江韩文霆杨振超西北农林科技大学机械与电子工程学院陕西杨凌712100 西北农林科技大学园艺学院陕西杨凌712100

光合速率及蒸腾速率是植物的2个重要生理指标。在全人工环境下,选取意大利生菜作为对象,设计并开展多环境变量对生菜光合速率及蒸腾速率影响的嵌套实验,得到环境因子对生菜光合速率及蒸腾速率的影响规律,应用神经网络构建生菜幼苗期光... 详细信息

光合速率及蒸腾速率是植物的2个重要生理指标。在全人工环境下,选取意大利生菜作为对象,设计并开展多环境变量对生菜光合速率及蒸腾速率影响的嵌套实验,得到环境因子对生菜光合速率及蒸腾速率的影响规律,应用神经网络构建生菜幼苗期光合速率及蒸腾速率预测模型。针对幼苗期生菜,选择温度、相对湿度、光子通量密度(Photosynthetic photon flux density, PPFD)及CO_(2)浓度共4个环境影响因素,采用随机森林方法对数据进行相关性分析。结果表明,与蒸腾速率相关性由大到小的因素依次为CO_(2)浓度、温度、相对湿度、PPFD,与光合速率相关性由大到小的因素依次为CO_(2)浓度、PPFD、温度、相对湿度;采用枚举法确定隐藏层节点数和训练函数,通过遗传算法优化BP神经网络的初始权值和阈值,构建GA-BP神经网络生理指标预测模型。应用测试数据对模型进行验证,光合速率及蒸腾速率预测值与实测值的决定系数分别为0.962 12、0.979 44,均方根误差(RMSE)分别为2.983 2μmol/(m^(2)·s)、0.001 435 8 mol/(m^(2)·s),表明GA-BP神经网络在模型精度和迭代次数方面性能显著提高。研究结果可为设施生菜生产环境调控提供有效依据。

关键词：设施生菜光合速率蒸腾速率预测模型相关性分析 GA-BP神经网络

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考虑绿色研发结果不确定性的供应链渠道模式选择研究

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运筹与管理 2024年第5期33卷 84-90页

作者：陈楠蔡建峰韩文婷马嫣然西北工业大学管理学院陕西西安710129 西安外国语大学经济金融学院陕西西安710119

本文研究了绿色技术研发结果不确定时制造商与零售商在传统线上平台以及区块链平台进行销售时的渠道模式选择问题。同时考虑服务水平差异性和营销努力水平,运用斯塔克伯格博弈和卡特尔模型,分析了研发结果不确定时基于传统线上平台和区... 详细信息

本文研究了绿色技术研发结果不确定时制造商与零售商在传统线上平台以及区块链平台进行销售时的渠道模式选择问题。同时考虑服务水平差异性和营销努力水平,运用斯塔克伯格博弈和卡特尔模型,分析了研发结果不确定时基于传统线上平台和区块链平台四种不同决策模式下的最优绿色技术研发水平、营销水平、服务水平、定价与利润,并对四种情形进行了比较分析。研究结果表明:(1)不论是传统线上平台还是区块链平台,绿色技术研发成功时的制造商与零售商的利润均高于研发失败时的利润。(2)制造商与零售商选择何种渠道模式主要取决于区块链平台带来的间接收益水平。(3)零售商的利润随着区块链平台带来的间接收益的下降而下降,当间接收益下降到一定程度时,零售商倾向于采取传统线上平台。

关键词：绿色技术研发不确定性区块链卡特尔模型渠道模式选择

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Highly Sensitive Fluorometric Assay Method for Acetylcholine- sterase Inhibitor Based on Nile Red-Adsorbed Gold Nanoparticles

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Chinese Journal of Chemistry 2013年第8期31卷 1072-1078页

作者： wenting han Shuzhen Liao Chonghua Zhang Huazhi Ding Zhaoyang Wu[*] Guoli Shen Ruqin Yu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha Hunan 410082 China Email: Zhaoyang Wu (zywu@***)[*]State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha Hunan 410082 China Tel. 0086-0731-88821989

A new sensitive fluorometric assay method for acetylcholinesterase （ACHE） and its inhibitor was developed us- ing a fluorescent dye, nile red （NR）. Due to the fluorescence resonance energy transfer between the NR and the gold nanoparticle （AuNPs）, the fluorescence was quenched. AChE can break down acetylthiocholine to produce a thiol-bearing compound, thiocholine. In the presence of thiocholine, the nile red is replaced from the AuNPs sur- faces and simultaneously transformed to a derivative of nile red. The fluorescence intensity of the derivative is much stronger than that of the native nile red with the same concentration and its maximum emission wavelength has a blue shift so that the sensor achieves a good signal-to-background ratio. In addition, when organophosphate pesticide （OPs） exists, the activity of AChE can be inhibited, the generation of thiocholine will be prevented and no fluorescence enhancement occurs. The results show that the method is sensitive to AChE and paraoxon with the de- tection limits of 0.2 mU/mL and 0.05 ng/mL, respectively.

关键词： nile red gold nanoparticle fluorescence acetylcholinesterase inhibitors

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融合裂缝形态信息的电阻率测井成像图空白条带填充方法

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石油地球物理勘探 2024年第3期59卷 533-542页

作者：夏文鹤韩玉娇郭全兴官文婷杨燚恺陈向东西南石油大学电气信息学院四川成都610500 中国石化石油工程技术研究院北京102200 中国石油西南油气田公司工程技术研究院四川成都610050 西南交通大学信息科学与技术学院四川成都610031

电阻率测井成像图中存在宽度和位置不定的空白条带,而目前常用的空白条带反距离加权插值填充法仅依靠像素点距离参数计算插值,往往导致填充后区域与实测区域纹理不连贯,纹理易呈现直线状,与实际状况不符。为此,将裂缝区域形态与分布信... 详细信息

电阻率测井成像图中存在宽度和位置不定的空白条带,而目前常用的空白条带反距离加权插值填充法仅依靠像素点距离参数计算插值,往往导致填充后区域与实测区域纹理不连贯,纹理易呈现直线状,与实际状况不符。为此,将裂缝区域形态与分布信息作为参量,构建插值计算模型,形成融合裂缝形态分布信息的空白条带填充法。该填充算法能根据插值点与裂缝形态拟合线的相对位置关系,动态调整参考点对插值点插值的权重系数,填充后的裂缝区域纹理连贯平滑,更符合裂缝实际形态和分布的规律。实际测井数据应用表明,相较于传统填充方法,采用该方法填充后的裂缝分布拟合线与霍夫变换后曲线相似度提升25%以上,不连贯填充点数量下降88%以上,性能提升明显。该方法的应用成果可为后续测井图像中各种裂缝的智能识别和精确分割提供更好的数据支撑。

关键词：电测井成像图空白条带填充井壁裂缝反距离迭代插值法

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基于“岗课赛证”融通的“车辆空调装置检修”课程教学改革研究与实践

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制冷与空调（四川） 2024年第3期38卷 433-436,442页

作者：李文婷韩威陕西铁路工程职业技术学院渭南714000

对“车辆空调装置”课程教学现状进行了分析,指出了在“岗课赛证”融通方面教学内容存在的弊病,基于此从教学内容重构、高质量教师团队建设和成绩替代三个方面提出了改革措施,构建了理实一体化课程教学模式,改革成效明显,为持续深化教... 详细信息

对“车辆空调装置”课程教学现状进行了分析,指出了在“岗课赛证”融通方面教学内容存在的弊病,基于此从教学内容重构、高质量教师团队建设和成绩替代三个方面提出了改革措施,构建了理实一体化课程教学模式,改革成效明显,为持续深化教育教学改革、全面提高教学质量的进一步研究提供了有效参考。

关键词： “岗课赛证”融通理实一体化教学改革车辆空调

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