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Heritable targeted inactivation of c-myca and c-mycb in zebrafish using ZFN and TALEN

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Heritable targeted inactivation of c-myca and c-mycb in zebr...

基于起振段波形在线判别电力系统功率振荡性质

2012全国发育生物学大会

作者： wenshuang jia,Qingshun Zhao Model Animal Research Center,MOE Key Laboratory of Model Animal for Disease Study,Nanjing University,Nanjing 210061

c-myc(myelocytomatosis oncogene),a well-known proto-oncogene,has been proved to play an important role in a variety of cell processes,including cell-cycle progression,cell proliferation,cell differentiation,survival,adhesion,and cell-size *** of c-myc function has been shown to cause hematological *** human patients with tumor,c-myc is found to be down regulated in many malignant *** results indicate that c-myc is critical for the pathogenesis of many types of mammalian tumors,such as *** c-myc(c-myca and c-mycb) genes have been identified in ***,little is known about the roles of c-myc in zebrafish early *** order to understand the roles of c-myc in zebrafish embryogensis,we generated c-myca and c-mycb knockout zebrafish by using ZFN and TALEN,*** turned out that both c-myca and c-mycb knockout zebrafish developed and grew normally. The results suggest that the two genes may have redundant *** are now generating c-myca and c-mycb double knockout zebrafish by crossing the c-myca knockout zebrafish with c-mycb knockout zebrafish to examine our hypothesis. Conclusions:We have generated c-myca and c-mycb knockout zebrafish by using ZFN and TALEN,***, none of the knockout zebrafish has defects on development and growth,suggesting the two genes may have redundant functions.

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中国电机工程学报 2013年第25期33卷 54-60,11页

作者：李莹贾文双李文锋刘增煌陶向宇王官宏中国电力科学研究院北京市海淀区100192

电力系统功率振荡的主要分为负阻尼和强迫型振荡2种。这2种振荡的表现形式很相似,但采取的应对措施差别很大。为快速有效地判别振荡性质从而采取适当的措施抑制功率振荡,提出了一种基于起振段波形的二次差分法及相应的判别准则。针对2... 详细信息

电力系统功率振荡的主要分为负阻尼和强迫型振荡2种。这2种振荡的表现形式很相似,但采取的应对措施差别很大。为快速有效地判别振荡性质从而采取适当的措施抑制功率振荡,提出了一种基于起振段波形的二次差分法及相应的判别准则。针对2类功率振荡的起因及特点,通过比较该方法计算的每个周期中功率最大值(或峰峰值)的一次差分和二次差分的符号,即可在功率振荡发生后的几个或十几个周波内快速判别振荡性质。最后,采用多个实际电网的振荡实例验证了该方法的有效性及实用性。

关键词：电力系统功率振荡负阻尼振荡强迫振荡二次差分法

Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish(Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases

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Heritable Targeted Inactivation of Myostatin Gene in Yellow ...

Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein

第十届中国实验动物科学年会

作者： Zhangji Dong jiachun Ge Kui Li Zhiqiang Xu Dong Liang Jingyun Li Junbo Li wenshuang jia Yuehua Li Xiaohua Dong Shasha Cao Xiaoxiao Wang jianlin Pan Qingshun Zhao Model Animal Research Center MOE Key Laboratory of Model Animal for Disease StudyNanjing University Freshwater Fisheries Research Institute of jiangsu Province NanjingChina College of Life Sciences Nanjing Normal University Freshwater Fisheries Research Institute of jiangsu Province

Yellow catfish(Pelteobagrus fulvidraco) is one of the most important freshwater aquaculture species in ***,its small size and lower meat yield limit its edible ***(MSTN) is a negative regulator of mammalian muscle ***,the function of Mstn in fish remains *** explore roles of mstn gene in fish growth and create a strain of yellow catfish with high amount of muscle mass,we performed targeted disruption of mstn in yellow catfish using engineered zinc-finger nucleases (ZFNs).Employing zebrafish embryos as a screening system to identify ZFN activity,we obtained one pair of ZFNs that can edit mstn in yellow catfish *** the ZFNs,we successfully obtained two founders(Founder July29-7 and Founder July29-8) carrying mutated mstn gene in their germ *** mutated mstn allele inherited from Founder July29-7 was a null allele (mstn) containing a 4 bp insertion,predicted to encode function null *** mutated mstn inherited from Founder July29- 8 was a complex type of mutation(mstn),predicted to encode a protein lacking two amino acids in the N-terminal secretory signal of ***,we obtained 6 mstn and 14 mstn yellow *** our best knowledge,this is the first endogenous gene knockout in aquaculture *** result will help in understanding the roles of mstn gene in fish.

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Isolation of yellow catfish β-actin promoter and generation...

Influence of Higher—Order Dispersions and Raman Delayed Response on Modulation Instability in Microstructured Fibres

第十届中国实验动物科学年会

作者： jiachun Ge Zhangji Dong Jingyun Li Zhiqiang Xu Wei Song Jie Bao Dong Liang Junbo Li Kui Li wenshuang jia Muzi Zhao Yongxiang Cai jiaxin Yang jianlin Pan Qingshun Zhao College of Life Sciences Nanjing Normal University Freshwater Fisheries Research Institute of jiangsu Province MOE Key Laboratory of Model Animal for Disease Study Model Animal Research CenterNanjing University Zhejiang Provincial Key Lab for Technology & Application of Model Organisms School of Life SciencesWenzhou Medical College

Yellow catfish(Pelteobagrus fulvidraco Richardson) is one of the most important freshwater farmed species in ***,its small size and slow growth rate limit its commercial *** genetic engineering has been a powerful tool to develop and improve fish traits for aquaculture,we performed transgenic research on yellow catfish in order to increase its size and growth *** PCR with degenerate primers,we cloned a genomic fragment comprising 5’-flanking sequence upstream of the initiation codon ofβ-actin gene in yellow catfish. The sequence is 1,017 bp long,containing the core sequence of proximal promoter including CAAT box, CArG motif and TATA *** the transgene construct Tg(beta-actin:eYFP) of the proximal promoter fused to enhanced yellow fluorescent protein(eYFP) reporter gene into zebrafish and yellow catfish embryos,we found the promoter could drive the reporter to express transiently in both embryos at early *** the offspring of five transgenic zebrafish founders developed from the embryos microinjected with Tg(ycbeta-actin:mCherry) or 19 yellow catfish founders developed from the embryos microinjected with Tg(beta-actin:eYFP),we obtained three lines of transgenic zebrafish and one transgenic yellow catfish,*** the expression patterns of the reporter genes in transgenic zebrafish(Tg(ycbeta-actin:mCherry)nju8/+) and transgenic yellow catfish(Tg{beta-actin:eYFP)nju 11/ +),we found the reporters were broadly expressed in both *** summary,we have established a platform to make transgenic yellow catfish using the proximal promoter of its ownβ-actin *** results will help us to create transgenic yellow catfish using "all yellow catfish" transgene constructs.

关键词： β-actin promoter Transgene Transgenic yellow catfish Transgenic zebrafish

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Chinese Physics Letters 2003年第6期20卷 852-854页

作者： wenshuang-Chun SUWen-Hua ZHANGHua FUXi-Quan QIANLie-jia FANDian-Yuan DepartmentofOpticalScienceandEngineering SchoolofInformationScienceandEngineeringFudanUniversityShanghai200433 DepartmentofCommunicationEngineering SchoolofComputerandCommunicationHunanUniversityChangsha410082

Modulation instability (MI) in microstructured photonic crystal fibres is studied by using an extended nonlinear Schrodinger equation. The roles of arbitrary higher-order dispersions and Raman delayed response in MI are identified. It is shown that all the odd-order dispersions contribute nothing to MI, whereas all the even-order dispersions not only affect the conventional instability regions but also may lead to the appearance of new MI regions. Raman delayed response makes MI occur in both the normal and anomalous dispersive regimes and exerts different influences on the Stokes and anti-Stokes components to some extent.

关键词： Photonic crystal fibers

云南省瑞丽市18例感染登革病毒妊娠期妇女临床特征回顾性分析

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中华地方病学杂志 2021年第9期40卷 752-755页

作者：贾文爽李曼沈加员杨忠平毛家荣尹小雄刘永华李杨思琪周红宁大理大学病原与媒介生物研究所(普洱分部) 云南普洱665000 云南省虫媒传染病防控研究重点实验室云南公共卫生与疾病防控协同创新中心云南省寄生虫病防治所普洱665000 瑞丽市人民医院感染科云南瑞丽678600 瑞丽市疾病预防控制中心传染病控制科云南瑞丽678400 昆明医科大学公共卫生学院 650500

目的了解云南省瑞丽市感染登革病毒(DENV)妊娠期妇女临床特征,为当地制定有效的孕妇登革热诊疗方案提供依据。方法选择2017、2018年瑞丽市人民医院收治住院的18例感染DENV妊娠期妇女作为观察组,根据观察组年龄范围选择18例感染DENV非妊... 详细信息

目的了解云南省瑞丽市感染登革病毒(DENV)妊娠期妇女临床特征,为当地制定有效的孕妇登革热诊疗方案提供依据。方法选择2017、2018年瑞丽市人民医院收治住院的18例感染DENV妊娠期妇女作为观察组,根据观察组年龄范围选择18例感染DENV非妊娠期妇女作为对照组,回顾性收集两组患者流行病学及临床资料。对两组流行病学特征、临床症状、实验室生化指标进行比较分析。结果观察组和对照组年龄[(27.9±5.3)比(27.9±5.1)岁]、住院天数[(6.8±1.6)比(6.6±2.0)d]比较,差异均无统计学意义(t=-0.032、0.495,P均>0.05),观察组中1例妊娠期妇女孕早期流产。两组发热均为18例(100.0%),头痛、全身肌肉酸痛、畏寒均为14例(77.8%),纳差均为15例(83.3%),恶心呕吐均为5例(27.8%),乏力分别为14(77.8%)、16例(88.9%);观察组皮疹1例(5.6%),对照组无皮疹患者。两组上述临床症状分布情况比较,差异均无统计学意义(P均>0.05)。入院第1天,观察组红细胞降低[61.1%(11/18)比5.6%(1/18)]、血红蛋白降低[50.0%(9/18)比16.7%(3/18)]、红细胞压积降低比例[61.1%(11/18)比16.7%(3/18)]均显著高于对照组(P均<0.05);入院第5天,观察组血红蛋白降低[33.3%(6/18)比5.6%(1/18)]、红细胞压积降低比例[33.3%(6/18)比5.6%(1/18)]均显著高于对照组(P均<0.05)。结论感染DENV妊娠期妇女红细胞、血红蛋白、红细胞压积降低均较明显,孕早期会出现流产的风险。提示相关部门应加强医护人员培训,对感染DENV妊娠期妇女早诊断、早治疗。

关键词：登革病毒妊娠期临床特征回顾性分析

甘草次酸金配合物通过HMGB1抑制肌腱干细胞氧化应激抗肌腱病作用研究

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中华中医药杂志 2024年第5期39卷 2160-2165页

作者：高媛媛孟嘉李福卫费文颖王君睿田浩源孙文爽邵江娟郑仕中南京中医药大学南京210023 东部战区总医院南京210002

目的:探讨甘草次酸金配合物(GA-Au)通过抑制高迁移率族蛋白B1(HMGB1)的核质易位调控自噬抑制肌腱干细胞(TSCs)氧化应激发挥抗肌腱病的作用。方法:MTT法筛选造模及给药浓度;Western Blot检测HMGB1、P62、BECN1的蛋白表达;还原型谷胱甘肽(... 详细信息

目的:探讨甘草次酸金配合物(GA-Au)通过抑制高迁移率族蛋白B1(HMGB1)的核质易位调控自噬抑制肌腱干细胞(TSCs)氧化应激发挥抗肌腱病的作用。方法:MTT法筛选造模及给药浓度;Western Blot检测HMGB1、P62、BECN1的蛋白表达;还原型谷胱甘肽(GSH)和丙二醛(MDA)试剂盒检测细胞内GSH、MDA水平;免疫荧光检测HMGB1的表达情况;构建HMGB1过表达质粒,验证GA-Au的作用;HE和Masson染色观察大鼠肌腱组织胶原排列。结果:与造模组比较,GA-Au给药缓解了氧化应激状态下,TSCs内GSH的耗竭和MDA的积聚。体内实验表明,给药组的大鼠肌腱组织较造模组胶原排列较为紧密,未出现明显的空泡化以及肌腱断裂的情况。结论:GA-Au通过抑制HMGB1核质易位调控自噬,抑制TSCs氧化应激从而发挥抗肌腱病作用。

关键词：甘草次酸金配合物肌腱病氧化应激自噬高迁移率族蛋白B1

关节镜下肩峰成形术联合富血小板血浆与关节镜下肩峰成形术治疗肩峰下撞击综合征的疗效比较

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中华创伤杂志 2023年第9期39卷 793-800页

作者：程强骆虎孙文爽孟嘉中国人民解放军东部战区总医院骨科南京210002

目的比较关节镜下肩峰成形术联合富血小板血浆(PRP)与关节镜下肩峰成形术治疗肩峰下撞击综合征(SAIS)的临床疗效。方法采用回顾性队列研究分析2020年1月至2021年12月东部战区总医院收治的30例SAIS患者的临床资料,其中男9例,女21例;年龄2... 详细信息

目的比较关节镜下肩峰成形术联合富血小板血浆(PRP)与关节镜下肩峰成形术治疗肩峰下撞击综合征(SAIS)的临床疗效。方法采用回顾性队列研究分析2020年1月至2021年12月东部战区总医院收治的30例SAIS患者的临床资料,其中男9例,女21例;年龄24~48岁[(34.3±9.8)岁]。左肩19例,右肩11例。15例仅行关节镜下肩峰成形术(单纯肩峰成形组);15例行关节镜下肩峰成形术,术后向肩峰下间隙注入4 ml PRP(肩峰成形联合PRP组)。比较两组术前及术后6周、3个月及末次随访时视觉模拟评分(VAS)、Constant⁃Murley评分和肩关节主动前屈、外展、内旋及外旋活动度。观察术后并发症发生情况。结果患者均获随访3~12个月[(9.0±2.6)个月]。术前、术后3个月及末次随访时两组VAS、Constant⁃Murley评分比较,差异无统计学意义(P均>0.05);术后6周肩峰成形联合PRP组VAS为(1.5±0.5)分,Constant⁃Murley评分为(80.3±6.2)分,低于或高于单纯肩峰成形组的(2.5±0.7)分、(58.1±7.6)分(P0.05);术后6周肩峰成形联合PRP组肩关节主动前屈为(158.1±26.3)°,外展为(146.2±27.4)°,内旋为(54.2±11.8)°,大于单纯肩峰成形组的(128.8±29.6)°、(111.0±28.6)°、(35.7±12.3)°(P0.05)。肩峰成形联合PRP组无切口感染、肩峰骨折等并发症发生;单纯肩峰成形组1例出现切口延期愈合,经换药处理后痊愈。结论与关节镜下肩峰成形术相比,关节镜下肩峰成形术联合PRP治疗SAIS可早期减轻疼痛、促进肩关节功能恢复、改善关节主动活动度、减少并发症。

关键词：肩撞击综合征富血小板血浆关节镜检查肩峰成形术

Brusselator反应扩散模型共存态的定性分析

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计算机工程与应用 2015年第23期51卷 65-67,77页

作者：索文爽贾云锋陕西师范大学数学与信息科学学院西安710062

运用Poincaré不等式、Schwarz不等式以及Young不等式,通过积分方法分析了一类化学反应扩散模型——Brusselator模型的共存态解,给出了模型共存态的一些性质。

关键词： Brusselator反应扩散模型共存态定性分析