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益气解毒方通过TGF-β1/SMAD3信号通路抑制鼻咽癌细胞增殖、迁移和侵袭

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中药新药与临床药理 2024年第7期35卷 935-943页

作者：郭利培张文青刘洁史红健何迎春王贤文范婧莹湖南中医药大学湖南长沙410208 中医药防治眼耳鼻咽喉疾病湖南省重点实验室湖南长沙410208

目的研究益气解毒方对鼻咽癌细胞增殖、迁移和侵袭的影响,并从转化生长因子β1(TGF-β1)/SMAD3信号通路探讨其对增殖、迁移和侵袭的作用机制。方法(1)将鼻咽癌细胞分为4组:溶剂对照组、益气解毒方0.5 mg·mL^(-1)组、益气解毒方1.0 mg·... 详细信息

目的研究益气解毒方对鼻咽癌细胞增殖、迁移和侵袭的影响,并从转化生长因子β1(TGF-β1)/SMAD3信号通路探讨其对增殖、迁移和侵袭的作用机制。方法(1)将鼻咽癌细胞分为4组:溶剂对照组、益气解毒方0.5 mg·mL^(-1)组、益气解毒方1.0 mg·mL^(-1)组、5-氟尿嘧啶(5-Fluorouracil,5-Fu)2μg·mL^(-1)组,采用实时无标记细胞功能分析仪(real time cellular analysis technology,RTCA)监测细胞增殖;创伤愈合实验检测细胞迁移;药物干预24 h后,侵袭小室法检测细胞侵袭;Western Blot法检测细胞β-catenin、E-cadherin、N-cadherin、TGF-β1、SMAD3蛋白的表达水平。(2)将鼻咽癌细胞分为5组:溶剂对照组、TGF-β110 ng·mL^(-1)组、TGF-β110 ng·mL^(-1)+益气解毒方1.0 mg·mL^(-1)组、益气解毒方1.0 mg·mL^(-1)组、LY320088210μmol·L-1组,采用实时无标记细胞功能分析仪监测细胞增殖;创伤愈合实验检测细胞迁移;药物干预24 h后,侵袭小室法检测细胞侵袭;Western Blot法检测细胞β-catenin、E-cadherin、N-cadherin、TGF-β1、SMAD3蛋白的表达水平。(3)随机将裸鼠分为模型组、益气解毒方组和5-Fu组。皮下部位注射细胞悬液制备鼻咽癌裸鼠移植瘤模型,基本成瘤后,5-Fu组每2 d腹腔注射1次,其余组每天灌胃给药1次,连续3周。每隔3 d测量瘤体体积1次;Western Blot法检测各组组织中β-catenin、E-cadherin、N-cadherin蛋白表达水平。结果与溶剂对照组比较,益气解毒方(0.5、1.0 mg·mL^(-1))组的细胞增殖曲线均下降,细胞迁移和侵袭能力均降低(P<0.05,P<0.01),且E-cadherin蛋白表达明显升高(P<0.01),β-catenin、N-cadherin、TGF-β1、SMAD3蛋白表达均明显降低(P<0.05,P<0.01)。与模型组比较,益气解毒方组的鼻咽癌移植瘤体积明显减小(P<0.05),且益气解毒方组移植瘤组织中的β-catenin和N-cadherin蛋白表达明显下调(P<0.05,P<0.01),E-cadherin蛋白表达明显上调(P<0.01)。加入TGF-β1信号通路激活剂和抑制剂后,与益气解毒方1.0 mg·mL^(-1)组比较,TGF-β110 ng·mL^(-1)+益气解毒方1.0 mg·mL^(-1)组的TGF-β1、SMAD3、β-catenin和N-cadherin蛋白表达水平均明显升高(P<0.05,P<0.01),且细胞增殖、迁移和侵袭的能力明显增强(P<0.01)。结论益气解毒方能够通过调控TGF-β1/SMAD3信号通路抑制鼻咽癌细胞的增殖、迁移和侵袭。

关键词：益气解毒方鼻咽癌 TGF-β1/SMAD3信号通路增殖迁移侵袭 BALB/c-nu裸鼠人高转移鼻咽癌5-8F细胞

高效自适应纳米乳液调驱技术在东北油田的应用

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中国石油大学胜利学院学报 2021年第4期35卷 50-57页

作者：王金铸龚雪峰刘发根张龙龙郭文庆王静静中国石油大学(华东)科学技术研究院山东东营257061

油田开发过程中高含水一直制约着油田高效开发,无效注水、高含水采出液水处理成本的增加大幅度提升吨油成本。由于油田高含水,为降低油田开发成本实施大面积关停,引进膨胀纳米乳液调驱新技术实施降水增油,基于分散架桥二次聚并机理,强... 详细信息

油田开发过程中高含水一直制约着油田高效开发,无效注水、高含水采出液水处理成本的增加大幅度提升吨油成本。由于油田高含水,为降低油田开发成本实施大面积关停,引进膨胀纳米乳液调驱新技术实施降水增油,基于分散架桥二次聚并机理,强化注水、调剖调驱,结合调驱技术室内实验及矿场实验,对实施技术进行优化,实现有效封堵水窜大孔道,并取得较好的降水增油效果,实现油田的成功复产和高效开发。

关键词：膨胀纳米乳液调驱东北油田

A systematic review of metabolomic profiling of gastric cancer and esophageal cancer

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Cancer Biology & Medicine 2020年第1期17卷 181-198页

作者： Sha Huang Yang guo Zhexuan Li Yang Zhang Tong Zhou Weicheng You Kaifeng Pan wenqing Li Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing) Department of Cancer EpidemiologyPeking University Cancer Hospital&InstituteBeijing 100142China Joint International Research Center of Translational and Clinical Research Beijing 100142China

Objective:Upper gastrointestinal(UGI)cancers,predominantly gastric cancer(GC)and esophageal cancer(EC),are malignant tumor types with high morbidity and mortality *** studies have focused on metabolomic profiling of UGI cancers in recent *** this systematic review,we have provided a collective summary of previous findings on metabolites and metabolomic profiling associated with GC and ***:A systematic search of three databases(Embase,PubMed,and Web of Science)for molecular epidemiologic studies on the metabolomic profiles of GC and EC was *** Newcastle–Ottawa Scale(NOS)was used to assess the quality of the included ***:A total of 52 original studies were included for review.A number of metabolites were differentially distributed between GC and EC cases and non-cases,including those involved in glycolysis,anaerobic respiration,tricarboxylic acid cycle,and protein and lipid *** acid,glucose,citrate,and fumaric acid were among the most frequently reported metabolites of cellular respiration while glutamine,glutamate,and valine were among the most commonly reported amino *** lipid metabolites identified previously included saturated and unsaturated free fatty acids,aldehydes,and ***,the key findings across studies to date have been inconsistent,potentially due to limited sample sizes and the majority being hospital-based case-control analyses lacking an independent replication ***:Studies on metabolomics have thus far provided insights into etiological factors and biomarkers for UGI cancers,supporting the potential of applying metabolomic profiling in cancer prevention and management efforts.

关键词： Gastric cancer esophageal cancer metabolomics Warburg effect biomarkers

宫角妊娠子宫破裂致呼吸心跳骤停成功抢救1例

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中国计划生育和妇产科 2024年第9期16卷 15-17页

作者：李文清郭红霞陆瑞仲艳娜南京中医药大学沭阳附属医院妇产科江苏沭阳223600

宫角妊娠曾被定义为罕见的异位妊娠[1],由于宫角血供丰富,一旦发生子宫破裂常致大量出血,可危及产妇生命。本文报道我院成功抢救宫角妊娠子宫破裂后导致呼吸心跳骤停患者1例。该患者系胚胎移植术后双胎妊娠,子宫破裂5天前有下腹痛症状,... 详细信息

宫角妊娠曾被定义为罕见的异位妊娠[1],由于宫角血供丰富,一旦发生子宫破裂常致大量出血,可危及产妇生命。本文报道我院成功抢救宫角妊娠子宫破裂后导致呼吸心跳骤停患者1例。该患者系胚胎移植术后双胎妊娠,子宫破裂5天前有下腹痛症状,多次来院就诊,检查后告知其有宫角妊娠可能,建议住院治疗,但患者及家属拒绝,最后子宫破裂、失血性休克、呼吸心跳骤停,病例罕见且危急。

关键词：宫角妊娠胚胎移植术后子宫破裂失血性休克呼吸心跳骤停

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马尾松天然次生林近自然经营研究

湖南林业科技 2018年第3期45卷 27-34,43页

作者：黄帆唐效蓉许忠坤郭文清杨骏张翼汪昌国陈清新湖南省林业科学院湖南长沙410004 江华县国有林场湖南江华425500 城步县林业科学研究所湖南邵阳422503

对湖南慈利县乔木层22年生马尾松天然次生林进行近自然经营,对目标树周围干扰树采用不同强度的处理,6年生长数据分析和根据NPV及现金流量表分析发现:(1)不同强度伐除干扰树对整体林分和目标树的树高生长增量影响极低,但对胸径生长增量... 详细信息

对湖南慈利县乔木层22年生马尾松天然次生林进行近自然经营,对目标树周围干扰树采用不同强度的处理,6年生长数据分析和根据NPV及现金流量表分析发现:(1)不同强度伐除干扰树对整体林分和目标树的树高生长增量影响极低,但对胸径生长增量影响较大。高强度伐除干扰树能提高整体林分的平均胸径生长增量,6年可达到3.6 cm;(2)经过人为干扰的目标树生长增量远超于未做干扰的其它树,一代和二代目标树平均胸径生长增量均高于其它树,达到3.0 cm和2.6 cm,尤其是阔叶树种人为干扰后生长迅速,最快的是樟树,6年平均胸径生长增量达到6.0 cm;(3)高强度的伐除干扰树对目标树经营效果最好,目标树生长增量远超其它处理强度,尤其是二代目标树6年平均胸径生长增量达到了4.1 cm,一代目标树和其它树也分别达到3.5 cm和2.9 cm;(4)以1 hm^2为计算单位,在100年限内人工林经营3次周期累计净现值为26 459元,马尾松目标树经营为37 158元,目标树经营超出人工林经营10 699元。随着经营年限增加目标树经营将进一步拉大与人工林经营的经济收益。

关键词：马尾松天然次生林近自然经营目标树

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14个不同产地藜麦种质染色体核型分析

作物杂志 2024年第3期 82-89页

作者：刘建霞王文庆薛乃雯郭绪虎马赛雅朱国芳温日宇山西大同大学山西大同037009 山西大同大学设施农业技术研发中心山西大同037009 山西农业大学玉米研究所山西忻州034000

为明确不同产地藜麦的染色体数目、核型特征及亲缘与进化关系,采用常规压片法制片比较分析了14个藜麦种质染色体核型特征,对其进行近似数聚类。结果表明,忻藜6的染色体数为2n=54,其余13个种质的染色体数为2n=36;染色体核型共有1A、1B、1... 详细信息

为明确不同产地藜麦的染色体数目、核型特征及亲缘与进化关系,采用常规压片法制片比较分析了14个藜麦种质染色体核型特征,对其进行近似数聚类。结果表明,忻藜6的染色体数为2n=54,其余13个种质的染色体数为2n=36;染色体核型共有1A、1B、1C和2B 4种,总体较为对称;核不对称系数范围为52.06%~59.76%。核型聚类分析结果显示,14个藜麦种质总体聚为两大类,藜麦1605单独为一类,其余13个藜麦种质为一类。来自同一地区的藜麦品种在亲缘关系和进化程度上较为相似,不同产地的藜麦品种因起源或在进化过程中受到外界环境干预程度不同,导致亲缘关系较远。该研究获得14个不同藜麦种质的核型信息,填充了有关藜麦染色体核型的数据库以及在细胞遗传学方面的研究。

关键词：藜麦染色体核型分析

Investigation of Heterogeneous Ice Nucleation on the Micro-Cubic Structure Superhydrophobic Surface for Enhancing Icing-Delay Performance

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Journal of Renewable Materials 2020年第12期8卷 1617-1631页

作者： Senyun Liu Qinglin Liu Xian Yi Yizhou Shen Long guo wenqing Hou Haifeng Chen Zhen Wang State Key Laboratory of Aerodynamics China Aerodynamics Research and Development CenterMianyang621000China Key Laboratory of Icing and Anti/De-icing China Aerodynamics Research and Development CenterMianyang621000China Department of Materials Chemistry Qiuzhen SchoolHuzhou UniversityHuzhou313000China College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing210016China

The aim of this study is to explore the heterogeneous ice nucleation behavior based on controllable micro-cubic array structure surfaces from the statistic *** this end,we firstly constructed a group of micro-cubic array structures on silicon substrates by a selective plasma etching *** grafting low-free-energy substance,the as-constructed micro-cubic array structure surfaces exhibited higher non-wettability with the water contact angle being up to 150°.On this basis,500 cycles of freezing and melting processes were accurately recorded to analyze the instantaneous ice nucleation behavior according to the statistical results of freezing *** a consequence,the statistical freezing temperature of the sample with micro-spacing distance of 40μm is as low as−17.13°*** microstructure configuration(conforming to Cassie-Baxter wetting regime)not only could entrap more air pockets,but also achieved lower solid-liquid contact area,resulting in lower ice nucleation rate(~2–3 orders of magnitude less than that on the flat substrate).Furthermore,the gradually increasing micro-spacing distance to 60μm would induce the transition from CassieBaxter to Wenzel wetting state,leading to higher freezing probability and ice nucleation *** complete understanding on microstructure configuration improving the ice nucleation will lay the foundation stone for the microstructure design of ice-repellent materials.

关键词： Microstructure configuration superhydrophobic ice nucleation antiicing/icephobic material

Active-layer evolution and （CH3NH3）3Bi2l9-based solar substrate efficiency improvement of cell on TiO2-deposited ITO

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Nano Research 2016年第10期9卷 2921-2930页

作者： Xinqian Zhang Gang Wu Zhuowei Gu Bing guo wenqing Liu Shida Yang Tao Ye Chen Chen Weiwei Tu Hongzheng Chen State Key Laboratory of Silicon Materials MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and Engineering Zhejiang University Hangzhou 310027 China Department of Chemical Engineering Xinjiang Agriculture University Urumqi 830052 China

We systematically investigated the development of film morphology and crystallinity of methyl-ammonium bismuth （Ⅲ） iodide （MA3Bi2I9） through one- step spin-coating on TiO2-deposited indium tin oxide （ITO）/glass. The precursor solution concentration and substrate structure have been demonstrated to be critically important in the active-layer evolution of the MA3Bi2I9-based solar cell. This work successfully improved the cell efficiency to 0.42% （average： 0.38%） with the mesoscopic architecture of ITO/compact-TiOdmesoscopic-TiO2 （meso-TiO2）/ MA3Bi2I9/2,2＇,7,7＇-tetrakis（N,N-di-4-methoxyphenylamino）-9,9＇spiro-bifluorene （spiro-MeOTAD）/MoO3/Ag under a precursor concentration of 0.45 M, which provided the probability of further improving the efficiency of the BiB＋-based lead-free organic-inorganic hybrid solar cells.

关键词： methyl-ammonium bismuth (Ⅲ) iodide organic-inorganic hybridsolar cell TiO2-depostied indium tinoxide (FRO) substrate morphology and crystallinity precursor solution concentration

Spin transport in epitaxial Fe3O4/GaAs lateral structured devices

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Chinese Physics B 2022年第6期31卷 736-740页

作者： Zhaocong Huang wenqing Liu Jian Liang Qingjie guo Ya Zhai Yongbing Xu School of Physics Southeast UniversityNanjing 211189China Spintronics and Nanodevice Laboratory Department of ElectronicsUniversity of YorkYork YO105DDUnited Kingdom York-Nanjing Joint Center in Spintronics School of Electronic Science and EngineeringNanjing UniversityNanjing 210023China Department of Electronic Engineering Royal Holloway University of LondonEghamSurrey TW200EXUnited Kingdom

Research in the spintronics community has been intensively stimulated by the proposal of the spin field-effect transistor(SFET),which has the potential for combining the data storage and process in a single *** we report the spin dependent transport on a Fe_(3)O_(4)/GaAs based lateral structured *** and antiparallel states of two Fe_(3)O_(4) electrodes are achieved.A clear MR loop shows the perfect butterfly shape at room temperature,of which the intensity decreases with the reducing current,showing the strong bias *** the spin-dependent transport properties in this architecture has strong implication in further development of the spintronic devices for room-temperature SFETs.

关键词： spin field-effect transistor spin injection and detection half metal magnetoresistance