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A multiple-target mRNA-LNP vaccine induces protective immunity against experimental multi-serotype DENV in mice

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Virologica Sinica 2022年第5期37卷 746-757页

作者： Lihong He wenqiang sun Limin Yang Wenjun Liu Jing Li CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Institute of Infectious Diseases Shenzhen Bay LaboratoryShenzhenGuangdong518000China University of Chinese Academy of Sciences Beijing100049China Institute of Microbiology Center for Biosafety Mega-ScienceChinese Academy of SciencesBeijing100101China

Dengue virus(DENV)is a mosquito-borne virus with a rapid spread to humans,causing mild to potentially fatal illness in hundreds of millions of people each *** to the large number of serotypes of the virus,there remains an unmet need to develop protective vaccines for a broad spectrum of the ***,we constructed a modified mRNA vaccine containing envelope domain III(E-DIII)and non-structural protein 1(NS1)coated with lipid *** multi-target vaccine induced a robust antiviral immune response and increased neutralizing antibody titers that blocked all four types of DENV infection in vitro without significant antibodydependent enhancement(ADE).In addition,there was more bias for Th1 than Th2 in the exact E-DIII and NS1-specific T cell responses after a single ***,intramuscular immunization limited DENV transmission in vivo and eliminated vascular *** findings highlight that chimeric allogeneic structural and non-structural proteins can be effective targets for DENV vaccine and that they can prevent the further development of congenital DENV syndrome.

关键词： Dengue virus(DENV) mRNA vaccine Envelope domain III(E-DIII) Non-structural protein 1(NS1) Multi-serotype Immune response

Dissection of heterotic loci for grain yield using interconnected chromosome segment substitution lines in rice

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The Crop Journal 2022年第2期10卷 323-331页

作者： Chaopu Zhang Yongjian sun Dianwen Wang wenqiang sun Yuye Yu Zhongli Hu Sibin Yu National Key Laboratory of Crop Genetic Improvement College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina State Key Laboratory of Hybrid Rice College of Life ScienceWuhan UniversityWuhan 430072HubeiChina

Heterosis contributes greatly to crop production, but the genetic basis of heterosis is not fully *** identify heterotic loci(HLs) for grain yield, 12 yield traits were evaluated in four rice(Oryza sativa L.)mapping populations: one parental population of chromosome segment substitution lines derived from a cross between the japonica cultivar Nipponbare and indica cultivar 9311 and three connected test populations in either a homozygous 9311 genetic background or a heterozygous background. A total of 390 HLs were detected for the measured traits in two environments. The genetic bases of heterosis differed between the backcross and testcross populations. At least 10 HLs were confirmed in F1 hybrids between9311 and near-isogenic lines, each of which carried a heterotic locus of interest in the same 9311 background. All 10 showed overdominant or dominant effects on grain yield and yield components. Among them, three were verified as being associated with yield heterosis and colocalized in the same regions as those containing previously reported heterosis-associated genes. Five HLs were identified to be promising candidate loci that could be used to achieve more than 15% yield heterosis in several commercial rice hybrids. These findings suggest the potential of indica or japonica introgression for increasing yield in hybrid rice breeding programs.

关键词： Rice Heterotic loci Grain yield Overdominance Commercial hybrids

Influence of CNN Structure Parameters on Blind Equalization of Shortwave Channels

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Wuhan University Journal of Natural Sciences 2021年第1期26卷 102-106页

作者： LIU Qi sun wenqiang RU Guobao GAN Liangcai School of Electronic and Information Wuhan UniversityWuhan 430072HubeiChina

Aiming at the severe inter-symbol interference and high bit error rate in short-wave fast time-varying channels,this paper designs a short-wave channel blind equalizer based on Convolution Neural Network(CNN),and analyzes the influence of parameters in CNN structure on channel equalization,such as the number of convolution layers,the depth of convolution layer and the size of the convolution kernel *** simulating two typical short-wave time-varying channel,Rayleigh flat fading and frequency selective fading channels,we have the following results:1)Compared with the Recurrent Neural Network(RNN)structure equalizer,the CNN has higher accuracy during the training process,the convergence speed is faster,and the stability after convergence is higher.2)Under the condition of simulation,the CNN-based short-wave channel blind equalizer designed in this paper can effectively extract input signal when using 2×3×3 convolution kernel size and 2-layer convolutional *** characteristics of the classification layer improve the equalization performance while reducing the complexity of CNN structure.3)For the short-wave channel,the error rate of Convolution Neural Network Equalizer(CNNE)is lower than that of Recurrent Neural Network Equalizer(RNNE)under the same SNR.

关键词： channel equalization short-wave fast time-varying channel Convolution Neural Network(CNN)

Hairy Leaf 6, an AP2/ERF Transcription Factor, Interacts with OsWOX3B and Regulates Trichome Formation in Rice

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Molecular Plant 2017年第11期10卷 1417-1433页

作者： wenqiang sun Dawei Gao Yin Xiong Xinxin Tang Xiongfeng Xiao Chongrong Wang Sibin Yu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 China

Trichome formation has been extensively studied as a mechanistic model for epidermal cell differentiation and cell morphogenesis in plants. However, the genetic and molecular mechanisms underlying trichome formation （i.e., initiation and elongation） in rice remain largely unclear. Here, we report an AP2/ERF transcription factor, Hairy Leaf 6 （HL6）, which controls trichome formation in rice. Functional analyses revealed that HL6 transcriptionally regulates trichome elongation in rice, which is dependent on functional OsWOX3B, a homeodomain-containing protein that acts as a key regulator in trichome initiation. Biochemical and molecular genetic analyses demonstrated that HL6 physically interacts with OsWOX3B, and both of them regulate the expression of some auxin-related genes during trichome formation, in which OsWOX3B likely enhances the binding ability of HL6 with one of its direct target gene, OsYUCCA5. Popu- lation genetic analysis indicated that HL6 was under negative selection during rice domestication. Taken together, our findings provide new insights into the molecular regulatory network of trichome formation in rice.

关键词： rice trichome development auxin AP2/ERF transcription factor WOX transcription factor

The self-assembled nanoparticle-based trimeric RBD mRNA vaccine elicits robust and durable protective immunity against SARS-CoV-2 in mice

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Signal Transduction and Targeted Therapy 2021年第10期6卷 3074-3084页

作者： wenqiang sun Lihong He He Zhang Xiaodong Tian Zhihua Bai Lei sun Limin Yang Xiaojuan Jia Yuhai Bi Tingrong Luo Gong Cheng Wenhui Fan Wenjun Liu Jing Li CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina Institute of Infectious Diseases Shenzhen Bay LaboratoryShenzhenGuangdongChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources&Laboratory of Animal Infectious Diseases College of Animal Sciences and Veterinary MedicineGuangxi UniversityNanningGuangxiChina University of Chinese Academy of Sciences BeijingChina Tsinghua-Peking Center for Life Sciences School of MedicineTsinghua UniversityBeijingChina Institute of Microbiology Center for Biosafety Mega-ScienceChinese Academy of SciencesBeijingChina

As COVID-19 continues to spread rapidly worldwide and variants continue to emerge,the development and deployment of safe and effective vaccines are urgently ***,we developed an mRNA vaccine based on the trimeric receptor-binding domain(RBD)of the SARS-CoV-2 spike(S)protein fused to ferritin-formed nanoparticles(TF-RBD).Compared to the trimeric form of the RBD mRNA vaccine(T-RBD),TF-RBD delivered intramuscularly elicited robust and durable humoral immunity as well as a Th 1-biased cellular *** further challenge with live SARS-CoV-2,immunization with a two-shot low-dose regimen of TF-RBD provided adequate protection in hACE2-transduced *** addition,the mRNA template of TF-RBD was easily and quickly engineered into a variant vaccine to address SARS-CoV-2 *** TF-RBD multivalent vaccine produced broad-spectrum neutralizing antibodies against Alpha(B.1.1.7)and Beta(B.1.351)*** mRNA vaccine based on the encoded self-assembled nanoparticle-based trimer RBD provides a referenee for the design of mRNA vaccines targeting SARS-CoV-2.

关键词： immunity vaccine protective

Theoretical Derivation of Thermal Value Equations for Heating Furnaces

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Open Journal of Applied Sciences 2012年第2期2卷 104-109页

作者： Hainan Wu wenqiang sun Jiuju Cai Institute of Thermal and Environmental Engineering Northeastern University Shenyang China

Based on thermal value theory, the aim of this paper is to deduce the theoretical formulas for evaluating the energy effective utilization degree in technological pyrological processes exemplified by metallurgical heating furnaces. Heat transfer models for continuous heating furnaces, batch-type heating furnaces, and regenerative heating furnaces are established, respectively. By analyzing the movement path of injected infinitesimal heat attached on steel or gas, thermal value equations of continuous, batch-type, and regenerative heating furnaces are derived. Then the influences of such factors as hot charging, gas preheating and intake time of heat on energy effective utilization degree are discussed by thermal value equations. The results show that thermal value rises with hot charging and air preheating for continuous heating furnaces, with shorter intake time when heat is attached on steel or longer intake time when heat is attached on gas for batch-type heating furnaces and that with more heat supply at early heating stage or less at late stage for regenerative heating furnaces.

关键词： Thermal Value Continuous Heating Furnace Batch-Type Heating Furnace Regenerative Heating Furnace Energy Utilization Degree Heat Transfer

Influence of Steam Recovery and Consumption on Energy Consumption per Ton of Steel

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Influence of Steam Recovery and Consumption on Energy Consum...

Rice SPL10 positively regulates trichome development through expression of HL6 and auxin-related genes

2011 2nd International Conference on Advances in Energy Engineering(ICAEE)

作者： Guangyu Ma Jiuju Cai Lihong Zhang wenqiang sun Institute of Thermal and Environmental Engineering Northeastern University Technology Center of Ansteel Co. Ltd. Environmental Protection Science Research Institute of the Guangxi Zhuang Autonomous Region

Steam recovery and consumption contributes greatly to energy consumption per ton of *** is studied in this paper that the relationship between energy consumption per ton of steel and steam recovery ＆ consumption,including main processes,auxiliary processes,diffusion and other users,*** the factors attributing to energy consumption per ton of steel is *** expressions of steam consumption per ton of steel and energy consumption per steam supply are proposed in this paper.

关键词： energy consumption per ton of steel steam recovery steam consumption energy consumption per steam supply

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Journal of Integrative Plant Biology 2021年第8期63卷 1521-1536页

作者： Jinjie Li Bo Tang Yingxiu Li Chenguang Li Minjie Guo Haiyang Chen Shichen Han Jin Li Qijin Lou wenqiang sun Peng Wang Haifeng Guo Wei Ye Zhanying Zhang Hongliang Zhang Sibin Yu Long Zhang Zichao Li State Key Laboratory of Agrobiotechnology/Beijing Key Laboratory of Crop Genetic Improvement College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing100193 China National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan430070 China College of Plant Protection China Agricultural UniversityBeijing100193 China

Trichomes function in plant defenses against biotic and abiotic stresses;examination of glabrous lines,which lack trichomes,has revealed key aspects of trichome development and *** of allelism in 51 glabrous rice(Oryza sativa)accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in ***,we report that OsSPL10 acts as a transcriptional regulator controlling trichome *** and transient expression analyses revealed that variation in the approximately 700-bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar *** of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL-HL6 *** with genotype OsSPL10^(WD-17993)/HL6 generated by crossing WD-17993 with NIL-HL6 also had fewer trichomes in the *** LEAF6(HL6)encodes another transcription factor that regulates trichome initiation and elongation,and OsSPL10 directly binds to the HL6 promoter to regulate its ***,the transcript levels of auxin-related genes,such as OsYUCCA5 and OsPIN-FORMED1b,were altered in OsSPL10 overexpression and RNAi transgenic *** tests using locusts(Locusta migratoria)demonstrated that non-glandular trichomes affect feeding by this *** findings provide a molecular framework for trichome development and an ecological perspective on trichome functions.

关键词： auxin complementary genes hairy leaves Oryza sativa OsWOX3B

A recombinant receptor-binding domain in trimeric form generates protective immunity against SARSCoV-2 infection in nonhuman primates

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The Innovation 2021年第3期2卷 94-102页

作者： Limin Yang Deyu Tian Jian-bao Han Wenhui Fan Yuan Zhang Yunlong Li wenqiang sun Yanqiu Wei Xiaodong Tian Dan-dan Yu Xiao-li Feng Gong Cheng Yuhai Bi Yong-tang Zheng Wenjun Liu CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early Warning(CASCIRE)CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID)Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 101408China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Kunming National High-Level Biosafety Research Center for Nonhuman Primates Center for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunming 650107China Institute of Infectious Diseases Shenzhen Bay LaboratoryShenzhen 518000China Tsinghua-Peking Center for Life Sciences School of MedicineTsinghua UniversityBeijing 100084China

A safe and effective vaccine is critical to combat the COVID-19 ***,we developed a trimeric SARS-CoV-2 receptor-binding domain(RBD)subunit vaccine candidate that simulates the natural structure of the spike(S)trimer *** with the RBD trimer-induced robust humoral and cellular immune responses,and a high level of neutralizing antibodies was maintained for at least 4.5 ***,the antibodies that were produced in response to the vaccine effectively cross-neutralized the SARS-CoV-2501Y.V2 variant(B.1.351).Of note,when the vaccine-induced antibodies dropped to a sufficiently low level,only one boost quickly activated the anamnestic immune response,conferring full protection against a SARSCoV-2 challenge in rhesus macaques without typical histopathological changes in the lung *** results demonstrated that the SARS-CoV-2 RBD trimer vaccine candidate is highly immunogenic and safe,providing long-lasting,broad,and significant immunity protection in nonhuman primates,thereby offering an optimal vaccination strategy against COVID-19.

关键词： COVID-19 SARS-CoV-2 RBD subunit vaccine NHPs variants