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Structural diversity of eukaryotic 18S rRNA and its impact on alignment and phylogenetic reconstruction

Protein & Cell 2011年第2期2卷 161-170页

作者： Qiang Xie Jinzhong Lin Yan Qin Jianfu Zhou wenjun bu Department of Zoology and Developmental Biology College of Life SciencesNankai UniversityTianjin 300071China National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China College of Information Technical Science Nankai University94 Weijin RoadTianjin 300071China

Ribosomal RNAs are important because they catalyze the synthesis of peptides and *** studies of the secondary structure of 18S rRNA have revealed the basic locations of its many length-conserved and lengthvariable *** recent years,many more sequences of 18S rDNA with unusual lengths have been documented in *** data make it possible to recognize the diversity of the secondary and tertiary structures of 18S rRNAs and to identify the length-conserved parts of 18S *** longest 18S rDNA sequences of almost every known eukaryotic phylum were included in this *** illustrated the bioinformatics-based structure to show that,the regions that are more length-variable,regions that are less length-variable,the splicing sites for introns,and the sites of A-minor interactions are mostly distributed in different parts of the 18S ***,this study revealed that some length-variable regions or insertion positions could be quite close to the functional part of the 18S rRNA of Foraminifera *** tertiary structure as well as the secondary structure of 18S rRNA can be more diverse than what was previously *** revealing how this interesting gene evolves,it can help to remove ambiguity from the alignment of eukaryotic 18S rDNAs and to improve the performance of 18S rDNA in phylogenetic *** nucleotides shared by Archaea and Eukaryota but rarely by Bacteria are also reported here for the first time,which might further support the supposed origin of eukaryote from archaeans.

关键词： secondary structure diversity tertiary structure diversity 18S rRNA Foraminifera Euglenida

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Notes on the genus Brachineura Rondani(Diptera: Cecidomyiidae), with description of three new species from China

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Zoological Systematics 2018年第1期43卷 103-108页

作者： Kelong Jiao Hao Wang Yuanhong Wang wenjun bu Department of Plant Protection College of Horticulture and Landscape Tianjin Agricultural University Institute of Entomology College of Life Sciences Nankai University

The generic diagnosis of Brachineura is revised to give an accurate and adequate redescription by adding detailed characters. First records of Brachineura in China, including three new species, namely B. sphaerica Jiao ＆ bu, sp. nov., B. physiphora Jiao ＆ bu, sp. *** B. prodolichata Jiao ＆ bu, sp. nov., are described and illustrated. The type specimens were deposited in the Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China（NKUM）.

关键词： Cecidomyiidae Brachineura new species new record China

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General review of the tribe Brachineurini(Diptera:Cecidomyiidae) with description of Pennaticoxita tauricornuta gen.& *** China

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Zoological Systematics 2016年第3期41卷 307-314页

作者： Kelong Jiao Peijin Han Yuanhong Wang wenjun bu Department of Plant Protection College of Horticulture and LandscapeTianjin Agricultural University Institute of Entomology College of Life SciencesNankai University

The paper provides a review of the tribe Brachineurini first with subtribal arrangement （Brachineurina subtribe, nov. and Brachyneurina subtrib, nov.） and a key to the genera. A new genus and species, Pennaticoxita tauricornuta Jiao ＆ bu, gen. ＆ sp. nov. from China is described with generic diagnosis, illustration and photograph. The type specimens are deposited in the Institute of Entomology, College of Life Sciences, Nankai University, Tianjin, China （NKUM）.

关键词： Cecidomyiidae Brachineurina Brachyneurina new genus new species key.

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Natural selection on various sites of ribosomal proteins: a cladistic view

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Zoological Systematics 2016年第1期41卷 1-47页

作者： Haoyang Wu Yang Liu Yanhui Wang Jinzhong Lin Qiang Xie wenjun bu Department of Zoology and Developmental Biology College of Life Sciences Nankai University National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences

Deducing the function of certain sites within a protein necessitates a priori recognition of the strength of selective pressure. Currently, statistical method is the only option to evaluate the degree of conservation. In the statistical framework, the types of selective pressure can be divided into classifications of negative, nearly neutral and positive. However, such quantitative methods may omit some crucial amino acid sites among the nearly neutral results. In this study, we propose that the cladistic information can be also important to evaluate the functional importance of various amino acid sites. The ribosomal proteins of 62 eukaryotic species were chosen as the case for statistical and cladistic analysis. The evolutionary changes of each site in the aligned sequences were matched on a currently well-accepted cladogram of eukaryotes. Hundreds of synapomorphic sites were discovered in various clades, in which only part of them were suggested to be potentially significant in the statistical framework. Notably, the mutation on His213 of RPL10 in human beings, which are synapomorphic in vertebrates but only be identified as being under neutral selection, is account for the disease Autism. Therefore, the cladistic information can be complementary to the statistical framework in understanding lineage-specific selection event. Additionally, the bias in the accumulation of apomorphic amino acids is significant when going from the Chordata to the Mammalia lineages. This study emphasizes the value of analyzing transcriptomic and proteomic data in a cladistic way to recognize the presence of group-specific selection on various sites in proteins.

关键词： Autapomorphy synapomorphy functional prediction.

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A new species in the genus Malcus Stål(Hemiptera:Heteroptera:Malcidae)from China based on morphological and molecular data

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Entomotaxonomia 2020年第4期42卷 252-257页

作者： Shujing WANG wenjun bu College of Life Sciences Nankai UniversityTianjin 300071China

Morphological and molecular data confirm a new species in genus Malcus from Xizang,*** zhengi *** described and *** and illustrations of the adult,critical morphological characteristics and pygophore are provided.

关键词： Lygaeoidea morphology molecular data taxonomy

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基于模糊控制的超静定、多变量耦合空气弹簧隔振系统姿态控制

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振动与冲击 2007年第11期26卷 174-177页

作者：卜文俊单树军胡宗成海军工程大学振动与噪声研究所武汉430033 海军驻431厂军代表室辽宁葫芦岛125004

针对超静定、多变量耦合空气弹簧隔振系统开展其姿态控制研究。先将超静定问题转化,经过分析控制对象模型,提出一种新的基于耦合特性识别的控制方法,并将该控制方法融入模糊控制器的设计,以实现多变量耦合系统姿态控制。最后,通过试验... 详细信息

针对超静定、多变量耦合空气弹簧隔振系统开展其姿态控制研究。先将超静定问题转化,经过分析控制对象模型,提出一种新的基于耦合特性识别的控制方法,并将该控制方法融入模糊控制器的设计,以实现多变量耦合系统姿态控制。最后,通过试验表明控制方法可行,并且所设计的控制系统具有较好静态、动态性能。

关键词：超静定多变量耦合空气弹簧姿态模糊控制

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A new species of Phamartes Bresseel & Constant(Phasmatodea: Diapheromeridae: Necrosciinae) from Vietnam

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Entomotaxonomia 2019年第3期41卷 188-191页

作者： Yanfei LI wenjun bu College of Life Sciences Nankai UniversityTianjing 300071China

This article describes one new species,Phamartes elongatus *** Cát Tiên National Park,*** of the adult and genitalia are provided and a key to the two know species is provided based on the female.

关键词： stick insects taxonomy key

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Biased heteroplasmy within the mitogenomic sequences of Gigantometra gigas revealed by sanger and high-throughput methods

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Zoological Systematics 2018年第4期43卷 356-386页

作者： Xiaoya Sun Yanhui Wang Pingping Chen Hesheng Wang Lixiang Lu Zhen Ye Yanzhuo Wu Teng Li wenjun bu Qiang Xie Institute of Entomology College of Life Sciences Nankai University Tianjin 300071 China Department of Ecology and Evolution College of Life Sciences Sun Yat-sen University Guangzhou 5102 75 Guangdong China State Key Laboratory of Biocontrol Sun Yat-sen University Guangzhou 510275 Guangdong China Netherlands Biodiversity Centre Naturalis 2300 RA Leiden The Netherlands Authority of Bangxi Nature Reserve Baisha 572821 Hainan China Authority of Jianfengling National Nature Reserve Ledong 572542 Hainan China College of Life Sciences Lanzhou University Lanzhou 730000 China

Few studies have explored the differences between Sanger and HTS methods in the results of mitogenome sequencing. We used a single individual of insect to study the differences between the sequences given by Sanger and PCR-free HTS methods. Here we provided evidence for biased results of sequencing due to different methods in the mitochondrial genes of atp6, atp8, cox1, cox2, cox3, Cytb, nad2, nad3, nad4, nad5, rrn S, rrnL, trnH, trn I, and control region at various degrees. Especially, in cox1, the differently sequenced nucleotides account for 2.6% of the complete length. Furthermore, the highest value of the intraspecific genetic distance based on K2 P accounts for 2.5% using a barcode fragment size of cox1（651 bp, Sanger）, while the maximum distance of the corresponding cox1 fragment obtained by the two sequencing methods was 5.0%. We revealed that the methods of Sanger and HTS may give different sequencing results of mitochondrial genes, which may reflect the heteroplasmy of mitogenomes within an insect individual. Therefore, researchers should be very cautious in using the mixed data of a gene given by different methods of sequencing.

关键词： Sanger and high-throughput sequencing mitogenome coxl CO1 heteroplasmy,giant water strider

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南京某办公园区雨水回收利用系统设计

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给水排水 2022年第S1期48卷 387-390页

作者：卜文浚中交第三航务工程勘察设计有限公司上海200032

以南京某办公园区项目的雨水回收利用系统设计为依托,针对园区总调蓄能力不足的问题,采用设置收集池的方法,以满足海绵城市建设规划控制目标的要求,并介绍本园区雨水回收利用的处理工艺及用水安全保障措施。

关键词：海绵城市雨水回收利用办公园区

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基于工况聚类优化的船舶辐射噪声评估方法

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华中科技大学学报（自然科学版） 2020年第10期48卷 63-68,103页

作者：李瑞彪何琳卜文俊海军工程大学振动与噪声研究所湖北武汉430033 海军工程大学船舶振动噪声重点实验室湖北武汉430033

针对现有船舶工况数据输入不能满足工况传递路径分析(OTPA)辐射噪声评估方法线性系统条件,从而导致部分频段辐射噪声评估误差较大的问题,提出基于聚类优化的船舶辐射噪声评估方法.该方法通过改进的双向二维主成分分析(2DD-PCA)法提取工... 详细信息

针对现有船舶工况数据输入不能满足工况传递路径分析(OTPA)辐射噪声评估方法线性系统条件,从而导致部分频段辐射噪声评估误差较大的问题,提出基于聚类优化的船舶辐射噪声评估方法.该方法通过改进的双向二维主成分分析(2DD-PCA)法提取工况聚类特征,并利用K-means进行工况聚类,以聚类后的工况作为OTPA输入并评估辐射噪声.试验结果表明:基于聚类优化的船舶辐射噪声评估方法评估辐射噪声,1/3倍频程评估误差最大为2.1 dB,绝大部分频带小于1 dB,较OTPA方法下降约6.8 dB,验证了该方法的有效性.

关键词：辐射噪声工况传递路径分析工况聚类主成分分析 K-means

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