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BZW1高表达促进胃癌细胞的侵袭和转移:基于调控Wnt//β-catenin通路和促进上皮间质转化

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南方医科大学学报 2024年第2期44卷 354-362页

作者：张文静张诺杨子张小凤孙奥飞王炼宋雪耿志军李静胡建国蚌埠医学院第一附属医院检验科安徽蚌埠233004 蚌埠医学院第一附属医院胃肠外科安徽蚌埠233004 蚌埠医学院第一附属医院中心实验室安徽蚌埠233004 炎症相关性疾病基础与转化研究安徽省重点实验室安徽蚌埠233004

目的明确碱性亮氨酸拉链蛋白1(BZW1)在胃癌中的表达情况,并探讨其对胃癌患者预后的影响及其可能的作用机制。方法分别采用TIMER、UALCAN和Kaplan-Meier Plotter数据库分析BZW1在胃癌组织中的表达情况,与肿瘤分级、分期之间的相关性及对... 详细信息

目的明确碱性亮氨酸拉链蛋白1(BZW1)在胃癌中的表达情况,并探讨其对胃癌患者预后的影响及其可能的作用机制。方法分别采用TIMER、UALCAN和Kaplan-Meier Plotter数据库分析BZW1在胃癌组织中的表达情况,与肿瘤分级、分期之间的相关性及对患者预后的影响。纳入2014年1月~2016年12月在我院行胃癌根治术的102例患者,分析BZW1在胃癌组织中的表达水平、对胃癌疾病进展和患者术后5年生存率的影响。体外采用慢病毒转染的方式构建上调和下调BZW1表达的胃癌细胞系(MGC803),分析BZW1对MGC803细胞迁移、侵袭及上皮-间质转化(EMT)的作用。采用KEGG富集分析预测BZW1在胃癌中的可能作用机制,并进一步采用Western blot实验进行体外验证。结果生物信息学分析结果显示,BZW1在胃癌和癌旁组织中的表达量差异有统计学意义(P<0.01),免疫组化和RT-qPCR结果显示,BZW1在胃癌组织中的蛋白和mRNA表达水平分别是癌旁组织的3.30倍和6.54倍(P<0.01);胃癌组织中BZW1的表达水平与外周血CEA和CA199水平呈正相关(P<0.01);单因素结合Cox多元回归模型分析证实BZW1高表达(P<0.05,HR=2.070,95%CI:1.021~4.196)是影响胃癌患者根治术后5年生存率的独立危险因素;以BZW1相对表达量3.61为截点值,预判术后5年死亡的敏感性为75.56%,特异性为71.93%(P<0.01)。Transwell实验显示,上调BZW1可促进MGC803细胞的迁移和侵袭(P<0.05),下调则相反(P<0.05)。Western blot实验发现,上调BZW1可促进MGC803细胞N-cadherin与Vimentin的表达(P<0.05),并抑制E-cadherin的表达(P<0.05),下调则反之(P<0.05)。富集分析显示,BZW1生物功能可能与Wnt//β-catenin信号相关。Western blot实验进一步证实,上调BZW1可促进Wnt3a、β-Catenin及C-myc的表达(P<0.05),而下调则相反(P<0.05);使用Wnt通路抑制剂XAV-939可显著削弱上调BZW1对MGC803细胞中EMT关键分子的蛋白N-cadherin、Vimentin及E-cadherin的调控作用(P<0.05)。结论BZW1在胃癌组织中高表达并影响患者预后,可能与调控Wnt/β-catenin信号促进胃癌细胞EMT进程相关。

关键词：胃癌碱性亮氨酸拉链蛋白1 预后上皮-间质转化 Wnt/β-catenin

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Reaction inside a viral protein nanocage： Mineralization on a nanoparticle seed after encapsulation via self-assembly

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Nano Research 2017年第10期10卷 3285-3294页

作者： wenjing zhang Zhi-Ping zhang Xian-En zhang Feng Li State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China University of Chinese Academy of Sciences Beijing 100049 China National Laboratory ofBiomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

Protein nanocages are ideal templates for the bio-inspired fabrication of nanomaterials due to several advantageous properties. During the mineralization of nanoparticles （NPs） inside protein nanocages, most studies have employed a common strategy： seed formation inside protein nanocages followed by seeded NP growth. However, the seed formation step is restricted to gentle reaction conditions to avoid damage to the protein nanocages, which may greatly limit the spectrum of seed materials used for NP growth. We put forward a simple route to circumvent such a limitation： encapsulation of a preformed NP as the seed via self-assembly, followed by the growth of an outer metal layer. Using such a method, we succeeded in mineralizing size-tunable Au NPs and Au@Ag core-shell NPs （〈10 nm in diameter） with narrow size distributions inside the virus-based NPs of simian virus 40. The present route enables the utilization of NPs synthesized under any conditions as the starting seeds for nanomaterial growth inside protein nanocages. Therefore, it potentially leads to novel bioinorganic chimeric nanomaterials with tailorable components and structures.

关键词： protein nanocages mineralization gold nanoparticles gold-silver core-shellnanoparticles self-assembly

Decadal variation and trend of the upper layer salinity in the South China Sea from 1960 to 2010

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Journal of Oceanology and Limnology 2022年第4期40卷 1333-1348页

作者： wenjing zhang Weiqiang WANG Huibin XING State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China University of Chinese Academy of Sciences Beijing 100049China Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou) Guangzhou 511458China South China Sea Information Center State Oceanic AdministrationGuangzhou 510301China South China Sea Marine Prediction Center State Oceanic AdministrationGuangzhou 510310China

Ocean salinity is an essential measurable indicator of water cycle and plays a crucial role in regulating ocean and climate *** Simple Ocean Data Assimilation(SODA)reanalysis product,substantial decadal variability of the salinity in the upper layer of the South China Sea(SCS)from 1960 to 2010 was *** show that a decadal variation of the upper layer salinity is *** upper layer(100 m)waters are found to freshen from 1960,become saltier during 1975 to 1995,and freshen again from 1995 to *** strongest anomalies appear in the northeastern,northern,and northwestern regions in the three periods,*** salinity variation trends become weaker below the upper layer,except the salinifi cation trend in the northern SCS extends to at least 300 m during the salinifi cation *** of the salinity budget over the top 100 m shows that during the fi rst freshening period horizontal advection,vertical advection,and surface freshwater forcing all contribute to salinity freshening,and horizontal advection is relatively *** contribution of horizontal advection and surface freshwater forcing to the positive salinity anomaly is comparable,while the vertical advection is the secondary factor in the salinifi cation *** advection,especially zonal advection,plays a crucial role during the second freshening ***,horizontal advection is more important than that in the fi rst freshening *** addition,the contribution of horizontal advection is mainly in zonal direction controlled by Kuroshio *** analysis shows the upper-layer salinity variations have a Pacifi c Decadal Oscillation(PDO)-like signal,with freshening during the negative PDO years,and salinifi cation during the positive PDO *** mainly infl uences the upper-layer salinity changes through both atmospheric bridge and oceanic bridge.

关键词： salinity South China Sea(SCS) decadal variation trend salinity budget analysis

Environment drives the co-occurrence of bacteria and microeukaryotes in a typical subtropical bay

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Journal of Oceanology and Limnology 2023年第6期41卷 2292-2308页

作者： Yifan MA Lingfeng HUANG wenjing zhang State Key Laboratory of Marine Environmental Science Marine Biodiversity and Global Change Research CenterCollege of Ocean and Earth SciencesXiamen UniversityXiamen 361102China Xiamen Key Laboratory of Urban Sea Ecological Conservation and Restoration Xiamen UniversityXiamen 361102China Fujian Key Laboratory of Coastal Pollution Prevention and Control Xiamen UniversityXiamen 361102China Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems College of the Environment and EcologyXiamen UniversityXiamen 361102China

The co-occurrence of bacteria and microeukaryote species is a ubiquitous ecological phenomenon,but there is limited cross-domain research in aquatic *** conducted a network statistical analysis and visualization of microbial cross-domain co-occurrence patterns based on DNA sampling of a typical subtropical bay during four seasons,using high-throughput sequencing of both 18S rRNA and 16S rRNA ***,we found obvious relationships between network stability and network complexity *** example,increased cooperation and modularity were found to weaken the stability of cross-domain ***,we found that bacterial operational taxonomic units(OTUs)were the most important contributors to network complexity and stability as they occupied more nodes,constituted more keystone OTUs,built more connections,more importantly,ignoring bacteria led to greater variation in network ***,Alphaproteobacteria,Bacteroidetes,and Actinobacteria were the most ecologically important ***,we found that the environmental drivers most associated with cross-domain networks varied across seasons(in detail,the network in January was primarily constrained by temperature and salinity,the network in April was primarily constrained by depth and temperature,the network in July was mainly affected by depth,temperature,and salinity,depth was the most important factor affecting the network in October)and that environmental influence was stronger on bacteria than on microeukaryotes.

关键词： co-occurrence network cross-domain network stability network complexity subtropical bay

L-香芹酮基磺酸肟酯化合物的合成及其抑菌活性

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林产化学与工业 2024年第1期44卷 41-50页

作者：蒙玉培段文贵林桂汕张文静吴凯越广西大学化学化工学院广西南宁530004

以L-香芹酮(1)为原料,经盐酸羟胺肟化和O-磺酰化反应,合成得到23个未见文献报道的L-香芹酮基磺酸肟酯类化合物3a~3w,并通过~1H NMR、^(13)C NMR、FT-IR和HRMS对目标化合物的结构进行了表征。采用琼脂稀释法测试了目标化合物对8种植物病... 详细信息

以L-香芹酮(1)为原料,经盐酸羟胺肟化和O-磺酰化反应,合成得到23个未见文献报道的L-香芹酮基磺酸肟酯类化合物3a~3w,并通过~1H NMR、^(13)C NMR、FT-IR和HRMS对目标化合物的结构进行了表征。采用琼脂稀释法测试了目标化合物对8种植物病原菌的抑制活性。结果表明:在质量浓度50 mg/L时,目标化合物对所测试的8种植物病原菌均有一定的抑菌活性,其中化合物3b(R=o-FPh)、3c(R=m-FPh)和3h(R=p-ClPh)对苹果轮纹病菌的抑制率分别为76.7%、 75.9%和78.3%(均为B级活性水平),均优于阳性对照百菌清;化合物3h对黄瓜枯萎病菌、苹果轮纹病菌、玉米小斑病菌和西瓜炭疽病菌的抑制率分别为78.4%、 78.3%、 82.2%和78.8%(均为B级活性水平),具有一定的广谱抑菌活性;化合物3b对苹果轮纹病菌、玉米小斑病菌和西瓜炭疽病菌的抑制率分别为76.7%、 77.5%和78.6%(均为B级活性水平),也具有一定的广谱抑菌活性。

关键词： L-香芹酮磺酸肟酯抑菌活性合成

Subharmonic resonance of single-degree-of-freedom piecewise-smooth nonlinear oscillator

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Acta Mechanica Sinica 2020年第5期36卷 1109-1118,I0003页

作者： Jiangchuan Niu wenjing zhang Yongjun Shen Shaopu Yang State Key Laboratory of Mechanical Behavior and System Safety of Traffic Engineering Structures Shijiazhuang Tiedao UniversityShijiazhuang 050043China School of Mechanical Engineering Shijiazhuang Tiedao UniversityShijiazhuang 050043China

Take the single degree of freedom nonlinear oscillator with clearance under harmonic excitation as an example,the 1/3 subharmonic resonance of piecewise-smooth nonlinear oscillator is *** approximate analytical solution of 1/3 subharmonic resonance of the single-degree-of-freedom piecewise-smooth nonlinear oscillator is *** changing the solving process of Krylov-Bogoliubov-Mitropolsky(KBM)asymptotic method for subharmonic resonance of smooth nonlinear system,the classical KBM method is extended to piecewise-smooth nonlinear *** existence conditions of 1/3 subharmonic resonance steady-state solution are achieved,and the stability of the subharmonic resonance steady-statesolution is also *** is found that the clearance affects the amplitude-frequency response of subharmonic resonance in the form of equivalent negative *** a demonstration example,the accuracy of approximate analytical solution is verified by numerical solution,and they have good *** on the approximate analytical solution,the infuences of clearance on the critical frequency and amplitude-frequency response of 1/3 subharmonic resonance are analyzed in *** analysis results show that the KBM method is an effective analytical method for solving the subharmonic resonance of piecewise-smooth nonlinear *** it provides an effective reference for the study of subharmonicr esonance of other piecewise-smooth systems.

关键词： Subharmonic resonance Piecewise-smooth system Approximate analytical solution Asymptotic method

以药品监管理念为导向的基于近红外光谱与机器学习联用的艾叶真伪判别研究

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中国药学杂志 2024年第13期59卷 1238-1245页

作者：张凯笑熊婧郭涛王晓伟王海波李彦超张文静石岩河南中医药大学药学院郑州450046 河南省药品医疗器械检验院(河南省疫苗批签中心) 国家药品监督管理局中药材及饮片质量控制重点实验室郑州450008 中国食品药品检定研究院北京102629

目的以药品监管理念为导向,建立基于近红外光谱与机器学习联用的艾叶真伪品判别的方法。方法使用近红外光谱仪测定艾叶真伪样品的近红外光谱,并采用特征工程中的特征筛选、特征衍生等相关技术对实验数据进行处理。随机划分训练集和测试... 详细信息

目的以药品监管理念为导向,建立基于近红外光谱与机器学习联用的艾叶真伪品判别的方法。方法使用近红外光谱仪测定艾叶真伪样品的近红外光谱,并采用特征工程中的特征筛选、特征衍生等相关技术对实验数据进行处理。随机划分训练集和测试集,使用训练集样品数据对机器学习领域经典的逻辑回归模型进行2分类模式训练,测试集样品进行模型的评估。结果使用逻辑回归模型对测试集样品的判别准确率为97%,其他各项评价指标也均在92%以上。同时,对于正品与伪品混合样品的判别也较准确。相较于传统化学计量学方法,判别准确率更高。结论本研究所建立的逻辑回归模型可以实现鉴别艾叶的真伪,对药品监管工作具有技术支撑作用。

关键词：艾叶近红外光谱机器学习逻辑回归特征工程

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不同形态梗丝中烟碱含量波动分析

甘肃科技 2024年第8期40卷 78-80页

作者：张雯晶朱先约甘肃烟草工业有限责任公司技术中心甘肃兰州730050

研究探明片状梗、丝状梗、颗粒梗、再造梗中烟碱含量及波动差异情况,进而为掺梗烟支质量稳定性提升提供技术支撑。搭建了一种低样品需求质量情况下梗丝中烟碱的快速准确测定方法,测定了不同形态梗丝中烟碱的含量,结合方差分析等统计学... 详细信息

研究探明片状梗、丝状梗、颗粒梗、再造梗中烟碱含量及波动差异情况,进而为掺梗烟支质量稳定性提升提供技术支撑。搭建了一种低样品需求质量情况下梗丝中烟碱的快速准确测定方法,测定了不同形态梗丝中烟碱的含量,结合方差分析等统计学方法定量分析了梗丝形态对烟碱一致性及波动的影响情况。结果表明:(1)片状梗、丝状梗、颗粒梗、再造梗F值均小于F临界值,在0.05显著性水平时,各形态梗丝样品中的烟碱是均匀的。(2)片状梗、丝状梗、颗粒梗3种形态梗丝烟碱含量接近,再造梗烟碱含量显著高于其他梗丝,传统梗丝制造工艺对梗丝中烟碱含量无显著影响。(3)片状梗10次烟碱测定标准差为0.29~0.45 mg/g,丝状梗为0.09~0.18 mg/g,颗粒梗为0.05~0.09 mg/g,再造梗为0.11~0.19 mg/g。研究结论:相较于片状梗,将烟梗制备成丝状梗、颗粒梗、再造梗可显著降低梗丝中烟碱的波动,从而提升掺梗烟支产品质量的稳定性。

关键词：梗丝烟碱波动分析

年龄联合白蛋白-胆红素评分对经颈静脉肝内门体分流术后显性肝性脑病的预测价值

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实用临床医药杂志 2024年第5期28卷 53-58页

作者：张文静甘建和苏州大学附属第一医院感染科江苏苏州215000

目的探讨年龄联合白蛋白-胆红素(ALBI)评分对肝硬化伴食管胃底静脉曲张破裂出血(EGVB)患者经颈静脉肝内门体分流术(TIPS)治疗后显性肝性脑病(OHE)的预测价值。方法回顾性分析87例接受TIPS治疗的肝硬化伴EGVB患者的临床资料,术后随访6个... 详细信息

目的探讨年龄联合白蛋白-胆红素(ALBI)评分对肝硬化伴食管胃底静脉曲张破裂出血(EGVB)患者经颈静脉肝内门体分流术(TIPS)治疗后显性肝性脑病(OHE)的预测价值。方法回顾性分析87例接受TIPS治疗的肝硬化伴EGVB患者的临床资料,术后随访6个月,根据是否发生OHE将患者分为OHE组27例和非OHE组60例。通过单因素分析筛选出2组间差异有统计学意义的指标,对其进行二元Logistic回归分析,明确OHE发生的独立影响因素。绘制受试者工作特征(ROC)曲线,评估各独立影响因素单独及联合预测OHE的效能,应用MedCalc软件比较不同曲线下面积(AUC)的差异。结果87例患者中,27例患者术后6个月内发生OHE,发病率为31.03%。单因素分析结果显示,OHE组年龄、白蛋白、血钠、ALBI评分与非OHE组比较,差异有统计学意义(P0.05);年龄联合ALBI评分预测的AUC大于年龄、ALBI评分单独预测的AUC,差异有统计学意义(P<0.05)。结论年龄、ALBI评分为肝硬化伴EGVB患者TIPS治疗后发生OHE的独立影响因素,两者联用对OHE具有较高的预测效能。

关键词：年龄白蛋白-胆红素评分肝硬化经颈静脉肝内门体分流术显性肝性脑病