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基于RIL和CSSL群体定位大豆脂肪酸组分QTL

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中国农业科学 2021年第15期54卷 3168-3182页

作者：渠可心韩露谢建国潘文婧张泽鑫辛大伟刘春燕陈庆山齐照明东北农业大学农学院大豆遗传改良实验室

【目的】大豆(Glycine max)原产于中国,高品质的大豆在食品、饲料、纺织品等多种加工业中广泛应用,因此,选育高品质大豆已成为育种者和生产者的聚焦问题。通过对大豆脂肪酸各组分进行QTL定位及候选基因的筛选,为大豆品质改良奠定分子基... 详细信息

【目的】大豆(Glycine max)原产于中国,高品质的大豆在食品、饲料、纺织品等多种加工业中广泛应用,因此,选育高品质大豆已成为育种者和生产者的聚焦问题。通过对大豆脂肪酸各组分进行QTL定位及候选基因的筛选,为大豆品质改良奠定分子基础。【方法】以美国大豆品种Charleston和东农594为亲本构建重组自交系(RILs)、以栽培大豆绥农14与野生大豆ZYD00006为亲本构建染色体片段代换系(CSSLs)为试验材料。利用气相色谱法测定2个群体的脂肪酸含量,根据东北农业大学农学院大豆遗传改良实验室已构建的遗传图谱,通过Windows QTL Cartographer 2.5和ICIMapping软件对2017—2018年RIL群体与CSSL群体中的大豆脂肪酸组分进行QTL定位研究,并对所获得的QTL置信区间进行候选基因的挖掘。【结果】2017—2018年,RIL群体和CSSL群体分别定位到34和20个与脂肪酸组分相关的QTL,分布在除B2、C1、G、H、J、M和O以外的13个连锁群上。比较2个群体的QTL定位结果,发现在2个群体中重复检测到10对QTL,其中,分布在A1、C2、D1a、F、K和N连锁群上的QTL与多种脂肪酸含量相关,在A1连锁群上检测到亚油酸和油分含量重叠的QTL;在C2连锁群上检测到硬脂酸和油分含量重叠的QTL;在D1a连锁群上检测到硬脂酸和油分含量重叠的QTL;在F连锁群上检测到棕榈酸、硬脂酸和油分含量重叠的QTL;在K连锁群上检测到亚油酸和亚麻酸含量重叠的QTL;在N连锁群上检测到棕榈酸和油分含量重叠的QTL、油酸和亚油酸含量重叠的QTL。对QTL定位获得的所有置信区间进行候选基因的挖掘,从基因注释数据集中共筛选出485个候选基因,其中,271个候选基因具有GO注释,进一步进行GO富集数据分析,共有15个候选基因与脂肪酸相关。主要通过编码植物酰基-酰基载体蛋白(ACP)硫酯酶、脂肪酸去饱和酶、磷脂酶D1、脂肪酸-羟化酶、丙酮酸激酶和参与酰基辅酶A生物合成、调节脂肪酸链的延伸,从而影响脂肪酸的合成。【结论】共检测到54个与大豆脂肪酸各组分相关的QTL,在2个群体中重复检测到10对QTL,对QTL定位获得的置信区间进行候选基因的筛选,共有15个候选基因与脂肪酸相关。这些稳定的脂肪酸相关的QTL和脂肪酸相关的候选基因可用于大豆脂肪酸改良的分子标记辅助选择。

关键词：大豆导入系气相色谱法 QTL定位基因挖掘富集分析

Defect Engineering:Can it Mitigate Strong Coulomb Effect of Mg^(2+)in Cathode Materials for Rechargeable Magnesium Batteries?

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Nano-Micro Letters 2025年第1期17卷 135-159页

作者： Zhengqing Fan Ruimin Li Xin Zhang Wanyu Zhao Zhenghui pan Xiaowei Yang School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai 200240People’s Republic of China School of Chemistry and Chemical Engineering Taiyuan University of TechnologyTaiyuan 030024People’s Republic of China School of Materials Science and Engineering Tongji UniversityShanghai 201804People’s Republic of China

Rechargeable magnesium batteries(RMBs)have been considered a promising“post lithium-ion battery”system to meet the rapidly increasing demand of the emerging electric vehicle and grid energy storage ***,the sluggish diffusion kinetics of bivalent Mg^(2+)in the host material,related to the strong Coulomb effect between Mg^(2+)and host anion lattices,hinders their further development toward practical *** engineering,regarded as an effective strategy to break through the slow migration puzzle,has been validated in various cathode materials for *** this review,we first thoroughly understand the intrinsic mechanism of Mg^(2+)diffusion in cathode materials,from which the key factors affecting ion diffusion are further ***,the positive effects of purposely introduced defects,including vacancy and doping,and the corresponding strategies for introducing various defects are *** applications of defect engineering in cathode materials for RMBs with advanced electrochemical properties are also ***,the existing challenges and future perspectives of defect engineering in cathode materials for the overall high-performance RMBs are described.

关键词： Rechargeable magnesium battery Sluggish diffusion kinetic Defect engineering Cathode materials Ion migration

饥饿循环处理对黄河鲤新品系幼鱼补偿生长性能的影响

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上海海洋大学学报 2017年第5期26卷 699-705页

作者：苏胜彦盘文静张希昭黄才姬张成锋唐丹徐跑南京农业大学无锡渔业学院江苏无锡214081 中国水产科学研究院淡水渔业研究中心农业部淡水渔业与种质资源利用重点实验室江苏无锡214081

为探究黄河鲤新品系幼鱼在饥饿-饱食循环状态下的生长补偿特点,选用初始均质量(23.44±3.21)g的黄河鲤幼鱼360尾,随机分成4个实验组,每个实验组3个重复,每个重复30尾鱼,分别按处理组A组:饥饿5 d(S5)饱食20 d(F20);B组:S7-F28;C组:S15-F... 详细信息

为探究黄河鲤新品系幼鱼在饥饿-饱食循环状态下的生长补偿特点,选用初始均质量(23.44±3.21)g的黄河鲤幼鱼360尾,随机分成4个实验组,每个实验组3个重复,每个重复30尾鱼,分别按处理组A组:饥饿5 d(S5)饱食20 d(F20);B组:S7-F28;C组:S15-F60;对照组Con:F75的投喂方案饲养,养殖实验为75 d。研究表明:(1)经过不同时长的饥饿,在恢复饱食投喂阶段,A组的特定生长率(SGR)为1.70%,显著高于B组的1.08%、C组的0.87%以及对照组Con的0.60%;(2)在饥饿阶段,3个处理组A、B、C的体长特定生长率(SGR_L)显著低于对照组Con;当恢复投喂后,A组、B组的SGR_L则显著高于C组和对照组Con;(3)在饥饿阶段,A组的肥满度特定生长率(SGR_K)显著低于B组、C组和对照组Con,但在恢复投喂阶段,却显著高于B组、C组和对照组Con。(4)处理组A节省饲料率为8.7%,较另外两个处理组高。由试验结果可以看出,饥饿5 d饱食20 d循环3次的投喂方式能激发幼鱼产生部分补偿生长效应,且饲料投喂量最少。

关键词：特定生长率补偿生长饥饿黄河鲤新品系幼鱼

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某市中医复用诊疗器具委托消毒灭菌现况调查

当代医学 2024年第7期30卷 90-93页

作者：石果李瑞红潘文静夏培培郑州市中医院感染控制科河南郑州450007 郑州中医骨伤病医院感染控制科河南郑州450016

目的了解某市二级以下中医医疗机构可复用诊疗器具委托消毒灭菌现状,为市级中医医疗机构医院感染管理质控中心进行质控管理策略研究提供依据。方法回顾性分析2018至2019年度某市中医医疗机构双核心检查资料,选取某市开展中医侵入性诊疗... 详细信息

目的了解某市二级以下中医医疗机构可复用诊疗器具委托消毒灭菌现状,为市级中医医疗机构医院感染管理质控中心进行质控管理策略研究提供依据。方法回顾性分析2018至2019年度某市中医医疗机构双核心检查资料,选取某市开展中医侵入性诊疗技术且开展委托消毒灭菌的16所中医院作为研究对象,依据WS310.1-2016、WS310.2-2016、WS310.3-2016设计调查表进行现场调查。结果无污染器械收集暂存间、无污染器械清洗池、职业防护用品不齐全医院均为8所(53.33%),无温湿度设备医院9所(60.00%),温湿度不符合要求医院13所(86.67%);签订委托协议书医院15所(100.00%),无诊疗包配置清单、无消毒供应上岗证、相关法律法规文件不完善医院各8所(53.33%),人员岗位职责不明确、制度流程不完善医院各11所(73.33%);未建立追溯机制医院14所(93.33%),院内回收发放交接等文件记录不完善、无院外交接记录和无费用结算凭证医院各12所(80.00%)。结论某市一、二级中医医疗机构委托消毒灭菌工作处于起步阶段,缺乏规范、有效的质控管理,存在安全隐患,需通过科学的质控管理模式,提高中医复用诊疗器具处理质量,推动委托消毒灭菌工作持续改进。

关键词：中医医疗机构复用诊疗器具委托消毒灭菌

渤海海域新近纪湖盆萎缩期古水深恢复——以渤东低凸起南端为例

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海洋地质前沿 2019年第4期35卷 18-25页

作者：潘文静刘士磊田德瑞郭龙龙王晓玲王立红中海石油(中国)有限公司天津分公司天津300459 中海油能源发展股份有限公司天津300459

探井揭示渤海海域东南部地区新近系发育高频河湖交互相沉积体系,砂体预测难度大,其中古湖盆水体深度是影响该区砂体展布的重要因素之一。通过元素地球化学、地层频谱属性趋势分析技术、古生物等多种技术方法对新近纪古湖盆水体特征进行... 详细信息

探井揭示渤海海域东南部地区新近系发育高频河湖交互相沉积体系,砂体预测难度大,其中古湖盆水体深度是影响该区砂体展布的重要因素之一。通过元素地球化学、地层频谱属性趋势分析技术、古生物等多种技术方法对新近纪古湖盆水体特征进行恢复,结合古生物的古水深指示意义完成了新近纪湖泊萎缩期古湖盆水体深度的定量研究。研究结果表明:蓬莱7地区新近系发育缓坡沉积的淡水湖泊湖平面波动频繁,馆陶组沉积早期古水深较浅约3m左右,馆陶组中后期古水深开始增加,并且呈现出从南向北逐渐加深的趋势。明化镇组下段中上部沉积时期古水深达最大,但明化镇组下段沉积时期水体深度主要在4～6m范围内波动。

关键词：渤海海域新近纪古生物古湖盆古水深

Wear behavior of copper material removal during fluid jet polishing:A comparative study between experiment and simulation

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Friction 2024年第8期12卷 1680-1695页

作者： wenjing ZHANG Xin ZHANG Tiancheng AI Dan GUO Guoshun pan State Key Laboratory of Tribology in Advanced Equipment Tsinghua UniversityBeijing 100084China Guangdong Provincial Key Laboratory of Optomechatronics Shenzhen 518057China

As a crucial part in micro-electromechanical manufacture,local ultra-precision processing of highly ductile copper is expected to be realized by fluid jet polishing(FJP),which widely utilized in optical *** copper exhibits different wear behavior from stiff and brittle material,there is currently no abrasive wear prediction model applicable for copper to investigate the polishing *** research reveals that the copper material removal is dominated by deformation wear rather than cutting wear through abrasive jet impact experiments and localized wear scars analysis.A three-dimensional gas-liquid-particle triphasic wear model for copper in FJP is developed by considering impact energy and wear mechanism ***,validation assessments at various working pressures and impingement angles achieve the goodness-of-fit up to 0.92–0.97 in quantitative comparison between simulations and experimental measurements,which demonstrate the wear prediction ability of the proposed *** investigation facilitates a better understanding of copper wear mechanism and provides theoretical guidance for FJP process optimization.

关键词： fluid jet polishing(FJP) wear mechanism computational fluid mechanics wear model material removal

Imperative for long-term management and surveillance in Kawasaki disease

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World Journal of Clinical Cases 2025年第4期13卷 61-63页

作者： Yan pan Fu-Yong Jiao Department of Pediatrics The First Affiliated Hospital of Yangtze UniversityJingzhou 434000Hubei ProvinceChina Shaanxi Kawasaki Disease Diagnosis and Treatment Center Children’s HospitalShaanxi Provincial People’s Hospital of Xi’an Jiaotong UniversityXi’an 710000Shaanxi ProvinceChina

Kawasaki disease(KD)is a significant pediatric vasculitis known for its potential to cause severe coronary artery *** the effectiveness of initial treatments,such as intravenous immunoglobulin,KD patients can experience long-term cardiovascular issues,as evidenced by a recent case report of an adult who suffered a ST-segment elevation myocardial infarction due to previous KD in the World Journal of Clinical *** editorial emphasizes the critical need for long-term management and regular surveillance to prevent such *** drawing on recent research and case studies,we advocate for a structured approach to follow-up care that includes routine cardiac evaluations and preventive measures.

关键词： Kawasaki disease Long-term management Coronary artery aneurysm Surveillance Preventive care

护联体内血液透析通路标准护理流程的建立及实践

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中华护理杂志 2022年第7期57卷 791-798页

作者：潘文静何文霞刘同强唐羽裳徐宇红杭琤韩惠芳丁钟琴陈成江苏省常州市第二人民医院血液净化中心 213000 江苏省常州市第二人民医院护理部 213000

目的探讨护联体内血液透析通路标准护理流程对维持性血液透析(maintained hemodialysis,MHD)患者动静脉内瘘的实施效果。方法2020年5月—2021年5月,以江苏省某三级甲等医院护联体内10所基层血液透析中心为试点,采用随机数字表法选取5所... 详细信息

目的探讨护联体内血液透析通路标准护理流程对维持性血液透析(maintained hemodialysis,MHD)患者动静脉内瘘的实施效果。方法2020年5月—2021年5月,以江苏省某三级甲等医院护联体内10所基层血液透析中心为试点,采用随机数字表法选取5所血液透析中心作为试验组,其余5所作为对照组。试验组实施构建的血液透析通路标准护理流程,对动静脉内瘘进行建立、评估、监测、维护等一体化的规范护理。对照组实施常规动静脉内瘘护理。比较实施前和实施后3、6、12个月,两组患者透析充分性、动静脉内瘘并发症发生情况和自体动静脉内瘘自我管理水平等指标。结果试验组143例,对照组155例完成研究。实施后的3、6、12个月,试验组的尿素清除指数高于对照组(P<0.05);实施后的6、12个月,试验组的血流量、尿素下降率和自体动静脉内瘘自我管理水平高于对照组(P<0.05);实施后的12个月,试验组的血肿和狭窄发生率低于对照组(P<0.05)。结论护联体内血液透析通路标准护理流程的建立,有效提高了MHD患者的透析充分性,提升了患者动静脉内瘘自我管理水平,并减少了动静脉内瘘并发症发生情况。

关键词：肾功能衰竭慢性血液透析动静脉内瘘血管通路护理护联体

Confinement and antenna effect for ultrasmall Y_(2)O_(3):Eu^(3+) nanocrystals supported by MOF with enhanced near-UV light absorption thereby enhanced luminescence and excellently multifunctional applications

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Nano Research 2021年第3期14卷 720-729页

作者： Hongyi Xu wenjing Yu Kai pan Guofeng Wang Peifen Zhu Key Laboratory of Functional Inorganic Material Chemistry Ministry of EducationSchool of Chemistry and Materials ScienceHeilongjiang UniversityHarbin150080China Department of Physics and Engineering Physics The University of TulsaTulsaOK74104USA

A novel host-guest luminous system with enhanced near-UV light absorption thereby enhanced luminescence are designed based on the synergism of quantum confinement,spatial confinement,and antenna effect,where ultrasmall Y_(2)O_(3):Eu^(3+)nanocrystals are fixed inside MOF(Eu/Y-BTC)as supporting *** Eu/Y-BTC not only limits the size and leads to lattice distortion of Y_(2)O_(3):Eu^(3+)nanocrystals and controls the distance between nanocrystals,but also promotes the light absorption and *** significantly red-shifted and broadened charge transfer band of Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)leads to the excellent applications of Y_(2)O_(3):Eu^(3+)in white light-emitting diodes(LEDs).Our results show that white light with superior color quality(CRI>90)and extremely high luminous efficacy(an LER of 335 lm/W)could be achieved using Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)as red *** Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)also improves the photoelectric performance of dye-sensitized solar cells(DSSCs),not only because Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)has a large specific surface area and the adsorption amount of the dye is increased,but also because the valence band position of Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)is 2.41 eV,which can provide an additional energy level between the TiO2 and dye,promoting electron *** these advantageous features,the multifunctional Y_(2)O_(3):Eu^(3+)/(Eu/Y-BTC)composite product will open new avenues in white LEDs and DSSCs.

关键词： confinement effect antenna effect enhanced luminescence ultrasmall Y_(2)O_(3):Eu^(3+)nanocrystals MOFs multifunctional applications