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RpUGT344J7 is involved in the reproduction switch of Rhopalosiphum padi with holocyclic life cycle

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Insect Science 2024年第4期31卷 1073-1089页

作者： Suji Wang wenjie huang Mengtian Li Ni Wang Xi Liu Maohua Chen Xiong Peng State Key Laboratory of Crop Stress Biology for Arid Areas Key Laboratory of Plant Protection Resources and Pest Management of Ministry of EducationKey Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural AffairsCollege of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi ProvinceChina

Many aphid species exhibit both cyclical parthenogenesis(CP)and the obligate parthenogenesis(OP)life history,which are genetically *** CP aphid lineages,the parthenogenetic individuals can switch from asexual to sexual reproduction quickly in response to environmental factors such as changes in photoperiod and ***,the OP aphid lineages do not undergo sexual reproduction under any *** far,mechanisms underlying the reproduction switch in CP aphids have not been fully *** padi,a serious worldwide insect pest of wheat,has both CP and OP *** diphosphate-glycosyltransferases(UGTs)are enzymes that participate in the metabolic detoxification of ***,we identified 43 RpUGT genes from *** genome and transcriptome sequences,and found that:(1)the UGT content of the CP lineage was significantly higher than that in the OP lineage at the key time points when CP lineage mainly produce virginoparae,gynoparae,and males under inducing condition,while there were no significant difference under normal conditions;(2)RpUGT344J7 gene was highly expressed during the time points when CP lineages produce gynopara and males;(3)the critical time points for CP lineages to produce virginoparaee,gynoparae,and males were affected when the CP lineages were injected with dsRpUGT344J7;(4)the knockdown of RpUGT344J7 caused a significant reduction in the total number of virginoparae,gynoparae,and males in the offspring under inducing *** findings contribute to our understanding of the molecular mechanisms underlying the quick shift from asexual to sexual reproduction in aphid species.

关键词： bird cherry-oat aphid cyclical parthenogenesis obligate parthenogenesis reproduction switch uridine diphosphate-glycosyltransferases(UGTs)

Phenolic profile， antioxidant properties， and beneficial gut microbiota associated with freeze dried Lycium ruthenicum

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Food Science and Human Wellness 2024年

作者： Jing huang Xueni Fan Xin Li Dingmei Dai wenjie huang Dong Ruan Shijuan Yan Xiaodan huang School of Public Health， Lanzhou University Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization， Agro-biological Gene Research Center， Guangdong Academy of Agricultural Sciences Institute of Animal Science， Guangdong Academy of Agricultural Sciences， Key Laboratory of Animal Nutrition and Feed Science in South China， Ministry of Agriculture and Rural Affairs， State Key Laboratory of Livestock and Poultry Breeding， Guangdong Key Laboratory of Animal Breeding and Nutrition

Black wolfberry (Lycium ruthenicum) is enriched in phytochemical metabolites which can benefit human health. However， few studies have examined the effects of different fruit drying methods on its polyphenol content， antioxidant activity， and anti-inflammatory activity. In addition， whether and how consuming dried black wolfberry affects gut microbiota has not been reported. Here， we assessed the phytochemical profile and bioactivities of black wolfberry dried through different methods， and subsequently characterized changes in human fecal microbiota associated with freeze-dried black wolfberry in vitro. The results showed that freeze-dried samples retained higher total polyphenols (49.68 ± 1.62) mg GAE/g， tannins (38.64 ± 1.35) mg GAE/g， and proanthocyanidins (3.35 ± 0.30) mg/g compared to sun drying or hot air drying (P < 0.05)， and exhibited higher antioxidant and anti-inflammatory activities. In bioreactor fermentations of human fecal inoculum， freeze-dried black wolfberry was associated with increased species richness and alpha diversity. At the genus level， fermentations treated with black wolfberry had higher abundance of Lactic Acid Bacteria including Lactococcus， Bifidobacterium， Lactobacillus， Pediococcus and Weissella， as well as butyrate-producing bacteria compared to the untreated samples， suggesting enrichment for taxa associated with a healthy gut microbiome. In addition， the black wolfberry treatment group had higher levels of short-chain fatty acids， which was consistent with PICRUSt2 inference. This study defines an optimal method for black wolfberry preservation to retain the beneficial compounds， and provides a foundation for further exploration of its potential benefits for human gut microbiota.

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The UDP-glycosyltransferase OsUGT706D2 positively regulates cold and submergence stress tolerance in rice

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The Crop Journal 2024年第3期12卷 732-742页

作者： Qing Liu Lanlan Zhang Shuwei Lyu Hang Yu wenjie huang Liqun Jiang Jing Zhang Bingrui Sun Xingxue Mao Pingli Chen Junlian Xing Wenfeng Chen Zhilan Fan Shijuan Yan Chen Li Guangdong Key Laboratory of New Technology in Rice Breeding/Guangdong Rice Engineering Laboratory/Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province) Ministry of Agriculture and Rural AffairsRice Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization Agro-biological Gene Research CenterGuangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina

In a genome-wide association study,we identified a rice UDP-glycosyltransferase gene,OsUGT706D2,whose transcription was activated in response to cold and submergence stress and to exogenous abscisic acid(ABA).OsUGT706D2 positively regulated the biosynthesis of tricin-4’-O-(syringyl alcohol)ether-7-O-glucoside at both the transcriptional and metabolic ***706D2 mediated cold and submergence tolerance by modulating the expression of stress-responsive genes as well as the abscisic acid(ABA)signaling *** of function of OsUGT706D2 increased cold and submergence tolerance and loss of function of OsUGT706D2 reduced cold *** positively regulated OsUGT706D2-mediated cold tolerance but reduced submergence *** findings suggest the potential use of OsUGT706D2 for improving abiotic stress tolerance in rice.

关键词： UDP-glycosyltransferase Rice Cold tolerance Submergence tolerance ABA

The light and hypoxia induced gene ZmPORB1 determines tocopherol content in the maize kernel

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Science China(Life Sciences) 2024年第3期67卷 435-448页

作者： Nannan Liu Yuanhao Du Shijuan Yan Wei Chen Min Deng Shutu Xu Hong Wang Wei Zhan wenjie huang Yan Yin Xiaohong Yang Qiao Zhao Alisdair R.Fernie Jianbing Yan National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China Agro-biological Gene Research Center Guangdong Academy of Agricultural SciencesGuangzhou 510640China College of Agronomy Hunan Agricultural UniversityChangsha 410128China College of Agronomy Northwest A&F UniversityXi'an 710000China State Key Laboratory of North China Crop Improvement and Regulation Hebei Sub-center of National Maize Improvement Center of ChinaCollege of AgronomyHebei Agricultural UniversityBaoding 071001China College of Life Sciences South-Central Minzu UniversityWuhan 430070China Plant Science Facility of the Institute of Botany Chinese Academy of SciencesBeijing 100093China National Maize Improvement Center of China Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100093China Shenzhen Key Laboratory of Synthetic Genomics Guangdong Provincial Key Laboratory of Synthetic GenomicsShenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany

Tocopherol is an important lipid-soluble antioxidant beneficial for both human health and plant growth. Here, we fine mapped a major QTLqVE1 affecting γ-tocopherol content in maize kernel, positionally cloned and confirmed the underlying gene ZmPORB1(por1), as a protochlorophyllide oxidoreductase. A 13.7 kb insertion reduced the tocopherol and chlorophyll content, and the photosynthetic activity by repressing ZmPORB1 expression in embryos of NIL-K22, but did not affect the levels of the tocopherol precursors HGA(homogentisic acid)and PMP(phytyl monophosphate). Furthermore, ZmPORB1 is inducible by low oxygen and light, thereby involved in the hypoxia response in developing embryos. Concurrent with natural hypoxia in embryos, the redox state has been changed with NO increasing and H_(2)O_(2) decreasing, which lowered γ-tocopherol content via scavenging reactive nitrogen species. In conclusion, we proposed that the lower lightharvesting chlorophyll content weakened embryo photosynthesis, leading to fewer oxygen supplies and consequently diverse hypoxic responses including an elevated γ-tocopherol consumption. Our findings shed light on the mechanism for fine-tuning endogenous oxygen concentration in the maize embryo through a novel feedback pathway involving the light and low oxygen regulation of ZmPORB1 expression and chlorophyll content.

关键词： maize tocopherol hypoxia photosynthesis ZmPORB1

多层结构聚合物基复合电介质材料储能特性研究进展

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高电压技术 2024年第6期50卷 2297-2308页

作者：查俊伟赵文帅黄文杰肖梦雨北京科技大学化学与生物工程学院北京100083

聚合物电介质材料由于其成本低、击穿强度高、可靠性高等优点被广泛用于电力系统。但是聚合物电介质材料放电能量密度较低,难以满足新一代小型化电容器的需求,多层结构具有能够协同提升介电常数和击穿强度的优势,解决聚合物电介质介电... 详细信息

聚合物电介质材料由于其成本低、击穿强度高、可靠性高等优点被广泛用于电力系统。但是聚合物电介质材料放电能量密度较低,难以满足新一代小型化电容器的需求,多层结构具有能够协同提升介电常数和击穿强度的优势,解决聚合物电介质介电常数与击穿强度之间的矛盾问题,实现具有优异储能特性的聚合物基电介质材料。文中综述了近年来多层结构聚合物基复合材料的研究进展,分析了多层结构中的极化与击穿现象,并详细讨论了聚合物基多层结构和无机层与聚合物基层结合的多层结构设计,归纳了一些能够提升多层结构聚合物基复合材料储能的方法。例如通过增加界面、优化多层结构中的填料分布以及构建过渡层的方法来提升储能特性。最后,对近年来多层结构聚合物基复合材料的进展进行了总结,并提出了未来的发展方向以及需要解决的关键问题。

关键词：电介质介电储能小型化电容器多层结构聚合物基复合材料

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论闽粤赣中央苏区红色音乐文化的生成场域

集美大学学报（哲社版） 2024年第4期27卷 85-92页

作者：黄文杰集美大学音乐学院福建厦门361021

中央苏区时期(1927—1934)的红色音乐是在社会变革的历史场域中产生的具有政治属性和阶级斗争内涵的革命音乐。在宏观历史和时代的经纬交合点上,马克思主义文艺理论指导下的文学艺术实践与本土民间文艺、外来艺术相互碰撞、彼此交融,促... 详细信息

中央苏区时期(1927—1934)的红色音乐是在社会变革的历史场域中产生的具有政治属性和阶级斗争内涵的革命音乐。在宏观历史和时代的经纬交合点上,马克思主义文艺理论指导下的文学艺术实践与本土民间文艺、外来艺术相互碰撞、彼此交融,促成生生不息的民间音乐在革命斗争的历史语境中投射于现实,此种嬗变之下,其性质和功能也产生了转化,本质上成了革命传统与历史逻辑的相互交织、文化传统与政治逻辑的相互契合,堪称文化再生产的典型事例。在功能和影响上,这一特定阶段的红色音乐发挥了强大的斗争用途和战时功用,是中国现代社会思想文化发展的伟大实践,也是“国家在场”的预演,同时成为具有中国特色的革命音乐文化的历史坐标和学理源泉。

关键词：中央苏区红色音乐文化斗争场域

基于目标检测仔猪乳头计数及乳房形态评估方法

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农业工程学报 2024年第3期40卷 156-164页

作者：李熙雅尹令黄文杰吴珍芳蔡更元田绪红华南农业大学数学与信息学院广州510642 华南农业大学动物科学学院广州510642 国家生猪种业工程技术研究中心广州510642 猪禽种业全国重点实验室广州510640

母猪乳头数量是生猪选育中重要参考指标之一,也是生猪繁殖表型数据的组成部分。成年母猪其腹部视频较难获取,且容易受污渍干扰,乳头自动点数实现难度较大,人工计数母猪乳头数工作量大、强度高、效率低、容易产生人为误差。鉴于猪仔从出... 详细信息

母猪乳头数量是生猪选育中重要参考指标之一,也是生猪繁殖表型数据的组成部分。成年母猪其腹部视频较难获取,且容易受污渍干扰,乳头自动点数实现难度较大,人工计数母猪乳头数工作量大、强度高、效率低、容易产生人为误差。鉴于猪仔从出生到成年乳头数量保持一致性,该研究提出了一种基于仔猪腹部视频的深度学习乳头计数及乳房形态评估方法。通过架设在仔猪初生护理平台上的相机拍摄仔猪腹部视频,根据清晰度筛选出细节清晰的帧序列图像集,经过数据预处理再使用改进Pignip-YOLOv5s目标检测网络对仔猪乳头进行自动计数。为提高计数准确率,帧序列图像集的乳头计数使用滑动窗口取众数得到最终计数结果。试验结果表明,改进的Pignip-YOLOv5s平均精度值(mean average precision, mAP)高达0.97,较YOLOv5原模型具备更高的鲁棒性。该研究方案在113段仔猪腹部视频数据集上测试得到仔猪乳头计数方法准确率达90.26%。同时该研究提出仔猪乳房形态评估参数乳头成对数、乳头间距,从而量化仔猪乳头形态表型特征,构建了母猪乳房外在形态指标,可为母猪选育和繁殖工作提供重要的参考依据。

关键词：模型深度学习视频计数 YOLOv5s 乳头计数乳房形态评估

连续时间完全-限定(K=2)两级轮询系统性能分析

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计算机工程 2024年第1期50卷 191-197页

作者：杨志军黄文洁丁洪伟云南大学信息学院云南昆明650500 云南省教育厅教学仪器装备中心云南昆明650223 云南师范大学教育部民族教育信息化重点实验室云南昆明650500

为了实现区分网络优先级、保证公平性、提高普通站点的性能和效率,在完全-限定(K=1)两级轮询控制系统模型的基础上,提出连续时间完全-限定(K=2)两级轮询控制系统模型。在该模型中,使用限定(K=2)服务和完全服务分别对普通站点和中心站点... 详细信息

为了实现区分网络优先级、保证公平性、提高普通站点的性能和效率,在完全-限定(K=1)两级轮询控制系统模型的基础上,提出连续时间完全-限定(K=2)两级轮询控制系统模型。在该模型中,使用限定(K=2)服务和完全服务分别对普通站点和中心站点进行服务。中心站点转换到普通站点进行服务时,使用捎带查询方式。在此基础上,采用马尔可夫链和概率母函数的数学方法建立该轮询系统模型,并推导平均排队队长和时延。利用MATLAB进行仿真实验,结果表明:理论值与仿真值误差较小,验证了理论分析的正确性;与门限-完全服务模型相比,该模型中心站点的队长和时延均小于门限-完全服务中心站点的队长和时延,具有更高的优先级;与一级完全服务和一级限定(K=2)服务模型相比,区分了优先级,性能分别提升11.7%和14.5%,说明两级服务远好于一级服务;与完全-限定(K=1)两级服务模型相比,增加了发送的数据,减少了等待时间,性能提升13.04%左右,进一步优化了普通站点的性能。

关键词：优先级连续时间完全-限定(K=2) 平均排队队长平均时延服务效率

基于无线通信的地铁列车在线灵活编组技术研究

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城市轨道交通研究 2024年第5期27卷 57-62页

作者：黄文杰陈志梁汝军陈美霞黄沈阅中车南京浦镇车辆有限公司南京市210031

[目的]为了响应国家“双碳”政策号召,在不降低地铁服务质量,提高列车低峰时段的满载率,减少能量的浪费,地铁业界迫切期待研制出具备快速、高效、在线动态灵活编组能力的地铁列车。[方法]针对现有灵活编组技术的不足点和成熟度,提出了... 详细信息

[目的]为了响应国家“双碳”政策号召,在不降低地铁服务质量,提高列车低峰时段的满载率,减少能量的浪费,地铁业界迫切期待研制出具备快速、高效、在线动态灵活编组能力的地铁列车。[方法]针对现有灵活编组技术的不足点和成熟度,提出了一种全新的基于无线通信的列车在线灵活编组技术。[结果及结论]详细介绍了该技术的工作原理、设备配置及其各自的功能,阐明了该技术的特点和优势,并对该技术的发展进行了展望。

关键词：地铁列车灵活编组无线通信系统方案系统组成