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Chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor promotes sciatic nerve repair

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Neural Regeneration Research 2014年第14期9卷 1358-1364页

作者： Yanru Zhang Hui Zhang Kaka Katiella Wenhua Huang Institute of International Education Zhengzhou University Institute of Clinical Anatomy Southern Medical University Department of Orthopedics First Affiliated HospitalZhengzhou University Institute of Clinical Medicine Zhengzhou University

A chemically extracted acellular allogeneic nerve graft can reduce postoperative immune rejection, similar to an autologous nerve graft, and can guide neural regeneration. However, it remains poorly understood whether a chemically extracted acellular allogeneic nerve graft combined with neurotrophic factors provides a good local environment for neural regeneration. This study investigated the repair of injured rat sciatic nerve using a chemically extracted acellular allogeneic nerve graft combined with ciliary neurotrophic factor. An autologous nerve anastomosis group and a chemical acellular allogeneic nerve bridging group were prepared as controls. At 8 weeks after repair, sciatic functional index, evoked potential amplitude of the soleus muscle, triceps wet weight recovery rate, total number of myelinated nerve fibers and myelin sheath thickness were measured. For these indices, values in the three groups showed the autologous nerve anastomosis group 〉 chemically extracted acellular nerve graft ＋ ciliary neurotrophic factor group 〉 chemical acellular allogeneic nerve bridging group. These results suggest that chemically extracted acellular nerve grafts combined with ciliary neurotrophic factor can repair sciatic nerve defects, and that this repair is inferior to autologous nerve anastomosis, but superior to chemically extracted acellular allogeneic nerve bridging alone.

关键词： nerve regeneration peripheral nerve injury chemically extracted acellular allogeneic nerve defect repair transplantation ciliary neurotrophic factor autologous nerve neural regeneration

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An Efficient Quantum Key Distribution Protocol with Dense Coding on Single Photons

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Computers, Materials & Continua 2019年第8期61卷 759-775页

作者： Hao Xiao Jun Zhang Wenhua Huang Mi Zhou Wencheng Hu School of Information Engineering Huzhou UniversityHuzhou313000China School of Information Engineering Jiangsu Maritime InstituteNanjing21100China College of Science Huzhou UniversityHuzhou313000China Virginia Commonwealth University Richmond Virginia23284USA College of Science Zhongyuan University of TechnologyZhengzhou450007China

Combined with the dense coding mechanism and the bias-BB84 protocol,an efficient quantum key distribution protocol with dense coding on single photons(QDKD-SP)is proposed.Compared with the BB84 or bias-BB84 protocols based on single photons,our QDKD-SP protocol has a higher capacity without increasing the difficulty of its experiment implementation as each correlated photon can carry two bits of useful information.Compared with the quantum dense key distribution(QDKD)protocol based on entangled states,our protocol is more feasible as the preparation and the measurement of a single-photon quantum state is not difficult with current technology.In addition,our QDKD-SP protocol is theoretically proved to be secure against the intercept-resend attack.

关键词： Quantum key distribution bias-BB84 dense coding mechanism quantum dense key distribution single photons

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生物3D打印在器官再造中的前沿热点和研究进展

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器官移植 2022年第2期13卷 161-168页

作者：黄文华南方医科大学基础医学院人体解剖学国家重点学科广东省医学生物力学重点实验室广东省医学3D打印应用转化工程技术研究中心广州510515

生物3D打印是采用生物材料和生物活性成分制作人工组织器官的新兴制造技术,已在多个医学领域广泛应用,在器官再造领域也具有突出的优势。近年来,生物3D打印器官取得了一系列重要突破,但目前仍处在研发和探索阶段,存在较多瓶颈,尚还不能... 详细信息

生物3D打印是采用生物材料和生物活性成分制作人工组织器官的新兴制造技术,已在多个医学领域广泛应用,在器官再造领域也具有突出的优势。近年来,生物3D打印器官取得了一系列重要突破,但目前仍处在研发和探索阶段,存在较多瓶颈,尚还不能用于体内移植。本文主要就3D打印技术的医学应用,生物3D打印技术的特点,生物3D打印器官在仿生结构、功能重建、免疫反应等方面的研究热点和难点,以及生物3D打印的最新研究进展,阐述生物3D打印技术在器官再造领域的应用前景,为器官重建、人工器官构建的研究及其临床应用提供新思路,以推动器官移植和个性化医疗的发展。

关键词：生物3D打印器官再造器官移植器官芯片免疫反应功能重建仿生结构血管化

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Combining acellular nerve allografts with brainderived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone

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Neural Regeneration Research 2014年第20期9卷 1814-1819页

作者： Yanru Zhang Hui Zhang Gechen Zhang Ka Ka Wenhua Huang School of International Education Zhengzhou University Institute of Clinical Anatomy Southern Medical University Department of Orthopedic Surgery First Affiliated Hospital of Zhengzhou University

In this study, we chemically extracted acellular nerve allografts from bilateral sciatic nerves, and repaired 10-mm sciatic nerve defects in rats using these grafts and brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells. Experiments were performed in three groups： the acellular nerve allograft bridging group, acellular nerve allograft ＋ bone marrow mesenchymal stem cells group, and the acellular nerve allograft ＋ brain-derived neurotrophic factor transfected bone marrow mesenchyrnal stem cells group. Results showed that at 8 weeks after bridging, sciatic functional index, triceps wet weight recovery rate, myelin thickness, and number of myelinated nerve fibers were significantly changed in the three groups. Variations were the largest in the acellular nerve allograft ＋ brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells group compared with the other two groups. Experimental findings suggest that chemically extracted acellular nerve allograft combined nerve factor and mesenchymal stem cells can promote the restoration of sciatic nerve defects. The repair effect seen is better than the single application of acellular nerve allograft or acellular nerve allograft combined mesenchymal stem cell transplantation.

关键词： nerve regeneration peripheral nerve regeneration peripheral nerve injury chemicallyextracted acellular nerve brain-derived neurotrophic factor bone marrow mesenchymal stem cells nerve tissue engineering neural regeneration

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FAM89A and IFI44L for distinguishing between viral and bacterial infections in children with febrile illness

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Pediatric Investigation 2021年第3期5卷 195-202页

作者： Shufeng Tian Jikui Deng Wenhua Huang Linlin Liu Yunsheng Chen Yongqiang Jiang Gang Liu Key Laboratory of Major Diseases in Children Ministry of EducationResearch Unit of Critical Infection in ChildrenChinese Academy of Medical Sciences2019RU016Department of Infectious DiseasesBeijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina Department of Infectious Diseases Shenzhen Children’s HospitalShenzhenGuangdongChina State Key Laboratory of Pathogens and Biosecurity Institute of Microbiology and EpidemiologyBeijingChina Department of Clinical Laboratory Shenzhen Children’s HospitalShenzhenGuangdongChina

Importance: The current lack of reliable rapid tests for distinguishing between bacterial and viral infections has contributed to antibiotic misuse.Objective: This study aimed to develop a novel biomarker assay that integratesFAM89A andIFI44L measurements to assist in differentiating between bacterial and viral infections.Methods: This prospective study recruited children with febrile illness from two hospitals between July 1, 2018, and June 30, 2019. A panel of three experienced pediatricians performed reference standard diagnoses of all patients (i.e., bacterial or viral infection) using available clinical and laboratory data, including a 28-day follow-up assessment. Assay operators were blinded to the reference standard diagnoses. The expression levels ofFAM89A andIFI44L were determined by quantitative real-time polymerase chain reaction assessment.Results: Of 133 potentially eligible patients with suspected bacterial or viral infection, 35 were excluded after the application of exclusion criteria. The resulting cohort included 98 patients: 59 with viral diagnoses and 39 with bacterial diagnoses. The areas under the curve (AUCs) of diagnoses usingFAM89A andIFI44L were 0.694 [95% confidence interval (CI): 0.583-0.804] and 0.751 (95%CI: 0.651-0.851), respectively. The disease risk score (DRS) [log2(FAM89A expression) - log2(IFI44L expression)] signature achieved an improved area under the receiver operating characteristic curve (AUC, 0.825;95%CI: 0.735-0.915), compared with the AUC generated from individual host RNA. A combination of the DRS and the C-reactive protein (CRP) level achieved an AUC of 0.896 (95%CI: 0.825-0.966). Optimal cutoffs for the DRS and CRP level were -3.18 and 19.80 mg/L, respectively.Interpretation: The DRS was significantly more accurate than the CRP level in distinguishing between bacterial and viral infections;the combination of these two parameters exhibited greater sensitivity and specificity. This study provides information that could be useful for the clinical application ofFAM89A a

关键词： FAM89A IFI44L Febrile children Bacterial infection Viral infection

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Plasma protein corona forming upon fullerene nanocomplex:Impact on both counterparts

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Particuology 2023年第2期73卷 26-36页

作者： Linjing Wu Fangqin Fu Wenhao Wang wenhua Wang Zhengwei huang Ying huang Xin Pan Chuanbin Wu College of Pharmacy Jinan UniversityGuangzhou510632China School of Pharmaceutical Sciences Sun Yat-Sen UniversityGuangzhou510006China

Protein corona refers to the structure composed of biomolecules adsorbed on the surface of nanomaterials.The study on the effect of the interaction between protein and nanoparticles can provide an important guide for the application of nanodrug delivery.To provide a reference for the research on fullerene(C60)nanocomplex drug delivery systems,this work studied the interaction between C60 nanocomplex and a variety of plasma proteins.Research showed that the protein binding with C60 nanocomplex did not change the charge properties of protein.The proteins induced the aggregation of C60 nanocomplex.The circular dichroism spectra showed that the secondary structure of the proteins changed after binding to C60 nanocomplex.The ultraviolet-visible spectra showed that the effect of C60 nanocomplex on proteins was concentration-dependent.The fluorescence spectra showed that C60 nanocomplex could intrinsic fluorescence alteration of proteins.The adsorption capacity of C60 nanocomplex to proteins was changed at 0 h and 4 h.The interaction between nanocomplex and proteins might affect the morphological characteristics of nanocomplex and the conformation of proteins.This work could provide a reference for the research and development of C60 nanocomplex and other carbon-based nanocomplex as nanoparticulate drug delivery systems.

关键词： Fullerene Nanocomplex Interaction Protein corona

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Post-marketing safety monitoring of Shenqifuzheng injection: a solution made of Dangshen(Radix Codonopsis)and huangqi(Radix Astragali Mongolici)

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Journal of Traditional Chinese Medicine 2014年第4期34卷 498-503页

作者： Qinghua Ai Wen Zhang Yanming Xie Wenhua Huang Hong Liang Hui Cao Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical Sciences National Engineering Research Center For Modernization of TCM Livzon (group) Limin Pharmaceutical Factory National Engineering Research Center for Modernization of TCM

OBJECTIVE:Toidentify the potential risk factors associated with Shenqifuzheng injection(SFI), a solution made of Dangshen(Radix Codonopsis) and huangqi(Radix Astragali Mongolici), for the timely provision of information to regulatory authorities.METHODS: A comprehensive analysis of the production process, quality standards, pharmacology,post-marketing clinical studies, and safety evaluation using the primary literature of adverse reactions(ADR), case analyses, and systematic reviews,intensive hospital safety monitoring of post-marketing drugs, and data provided by the hospital in-formationsystem(HIS).RESULTS: Sub-acute toxicity tests suggesting that a dose of 15 mL/kg(concentrated solution) had specific biological effects, whereas a smaller dose engendered no observable effects. Long-term toxicity testing in domestic rabbits showed that after SFI was administered for 90 days, the animals in each dosing group showed no chronic toxic reactions. Among 20 100 cases observed, the incidence of an ADR was 1.85‰. From March to November 2013,of the leading institutions and 22 sub-centers involved in the post-marketing clinical safety intensive hospital monitoring, 21 units completed 8484 cases of monitoring, and reported 23 cases of adverse reactions. No damage to renal function was found using SFI ata dosageanda treatment course larger and longer than that recommended for the adjuvant treatment of tumors. This could reduce the mortality rate of admitted patients based on the analysis of the data provided by the HIS. A total of 16 clinical case reports of adverse reactions related to SFI in 1999-2012 were obtained through literature retrieval. These reports contained information concerning 17 cases, with adverse reaction symptoms including thrombocytopenia, rash,chills,feeling cold,palpitation,dyspnea,edemaofa lower extremity, palpebral edema, and superficial vein in flammation,among others.CONCLUSION: This study introduces "get full access" to the flow of information on medicines regarding their ADR incidence rate and ch

关键词：安全性评价注射液党参上市黄芪监测扶正药物不良反应

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Influence of the structure and composition of Fe–Mn binary oxides on rGO on As(Ⅲ) removal from aquifers

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Journal of Environmental Sciences 2020年第2期32卷 133-144页

作者： Tao Sha wenhua Hu Jun Dong Zifang Chi Yongsheng Zhao Huazheng huang Key Lab of Groundwater Resources and Environment Ministry of EducationJilin UniversityChangchun 130021China

Fe–Mn binary oxide(FMBO) possesses high efficiency for As(Ⅲ) abatement based on the good adsorption affinity of iron oxide and the oxidizing capacity of Mn(Ⅳ), and the composition and structure of FMBO play important roles in this process.To compare the removal performance and determine the optimum formula for FMBO, magnetic graphene oxide(MRGO)–FMBO and MRGO–MnO2 were synthesized with MRGO as a carrier to improve the dispersity of the adsorbents in aquifers and achieve magnetic recycling.Results indicated that MRGO–FMBO had higher As(Ⅲ) removal than that of MRGO–MnO2,although the ratios of Fe and Mn were similar, because the binary oxide of Fe and Mn facilitated electron transfer from Mn(Ⅳ) to As(Ⅲ), while the separation of Mn and Fe on MRGO–MnO2 restricted the process.The optimal stoichiometry x for MRGO–FMBO(MnxFe3-xO4) was 0.46, and an extraordinary adsorption capacity of 24.38 mg/g for As(Ⅲ) was achieved.MRGO–FMBO showed stable dispersive properties in aquifers, and exhibited excellent practicability and reusability, with a saturation magnetization of 7.6 emu/g and high conservation of magnetic properties after 5 cycles of regeneration and reuse.In addition, the presence of coexisting ions would not restrict the practical application of MRGO–FMBO in groundwater remediation.The redox reactions of As(Ⅲ) and Mn(Ⅳ) on MRGO–FMBO were also described.The deprotonated aqueous As(Ⅲ) on the surface of MRGO–FMBO transferred electrons to Mn(Ⅳ), and the formed As(Ⅴ) oxyanions were bound to ferric oxide as inner-sphere complexes by coordinating their "–OH" groups with Mn(Ⅳ)oxides at the surface of MRGO–FMBO.This work could provide new insights into highperformance removal of As(Ⅲ) in aquifers.

关键词： MRGO–MnO2 MRGO–FMBO Optimum formula As(Ⅲ) abatement Adsorption–oxidation-collection

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Ginsenoside Rb1 attenuates activated microglia-induced neuronal damage

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Neural Regeneration Research 2014年第3期9卷 252-259页

作者： Lining Ke Wei Guo Jianwen Xu Guodong Zhang Wei Wang Wenhua Huang Department of Human Anatomy Histology and Embryology Southern Medical University Department of Human Anatomy Histology and Embryology Fujian Medical University

The microglia-mediated inflammatory reaction promotes neuronal damage under cerebral isch- emia/hypoxia conditions. We therefore speculated that inhibition of hypoxia-induced microglial activation may alleviate neuronal damage. To test this hypothesis, we co-cultured ginsenoside Rb 1, an active component of ginseng, and cortical neurons. Ginsenoside Rb l protected neuronal morphology and structure in a single hypoxic culture system and in a hypoxic co-culture system with microglia, and reduced neuronal apoptosis and caspase-3 production. The protective effect was observable prior to placing in co-culture. Additionally, ginsenoside Rbl inhibited levels of tumor necrosis factor-a in a co-culture system containing activated N9 microglial cells. Ginse-noside Rbl also significantly decreased nitric oxide and superoxide production induced by N9 microglia. Our findings indicate that ginsenoside Rbl attenuates damage to cerebral cortex neu-rons by downregulation of nitric oxide, superoxide, and tumor necrosis factor-a expression in hypoxia-activated microglia.

关键词： nerve regeneration traditional Chinese medicine ischemia/hypoxia microglia neurons apoptosis ginsenoside Rb l nerve inflammation factor NSFC grant neural regeneration

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The effect of organic ligand modification on protein corona formation of nanoscale metal organic frameworks

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Chinese Chemical Letters 2022年第9期33卷 4185-4190页

作者： Wenhao Wang Huihui Liu Zhengwei huang Fangqin Fu wenhua Wang Linjing Wu Ying huang Chuanbin Wu Xin Pan School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China College of Pharmacy Jinan UniversityGuangzhou 511443China

Nanoscale metal organic frameworks(NMOFs)have been widely reported in biomedical field for their unique porous structure and tunable multifunctionality.However,when administrated in vivo,the protein corona will be formed on the surface of NMOFs,significantly affecting their biodistribution,pharmacokinetics and drug release.Few studies paid attention to the protein corona formation process and its influencing factors of NMOFs.As a well-established strategy for altering structure features of NMOFs,the organic ligand modification may have effect on the protein corona formation process,which is to be investigated.In this study,the zirconium(Zr)-based UIO66 was chosen as model NMOFs,the organic ligand of which was modified with amino group(-NH_(2))or carboxyl group(-COOH)to synthesize UIO66-NH_(2)and UIO66-2COOH,respectively.Bovine serum albumin(BSA)was chosen as model protein to investigate the protein corona formation process of NMOFs.The current results showed that the-COOH modification remarkably enhanced the BSA adsorption on NMOFs while-NH_(2)slightly decreased the protein binding affinity.These differences may be ascribed to the two different dominate protein corona formation modes,i.e.,surface coating mode and porous embedded mode.The protein corona formation did not affect the crystal phase of NMOFs but increased the content ofα-helix of BSA.Ultimately,upon protein corona formation,the cellular uptake of NMOFs was significantly affected.We believe our study will provide a new research paradigm to the design and applications of NMOFs.

关键词： Protein corona NMOFs UIO66 Organic ligand modification BSA

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