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Frontiers of Environmental Science & Engineering 2020年第1期14卷 23-34页

作者： Kun Wan wenfang lin Shuai Zhu Shenghua Zhang Xin Yu Key Laboratory of Urban Environment and Health.Institute of Urban Environment Chinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China Beijing Guohuan Tsinghua Environmental Engineering Design&Research Institute Co. Ltd.Beijing 100084China

The bacterial antibiotic resistome(BAR)is one of the most serious contemporary medical *** BAR problem in drinking water is receiving growing *** this study,we focused on the distribution,changes,and health risks of the BAR throughout the drinking water treatment *** extracted the antibiotic resistance gene(ARG)data from recent publications and analyzed ARG profiles based on diversity,absolute abundance,and relative *** absolute abundance of ARG was found to decrease with water treatment processes and was positively correlated with the abundance of 16S rRNA(r=0.963,/?ling the diversity of ARG,while biofiltration,especially granular activated carbon filtration,increased the diversity of *** biofiltration and chlorination altered the structure of the resistome by affecting relative ARG *** addition,we analyzed the mechanism behind the impact of biofiltration and chlorination on the bacterial antibiotic *** intercepting influent ARG-carrying bacteria,biofilters can enrich various ARGs and maintain ARGs in *** further selects bacteria co-resistant to chlorine and ***,we proposed the BAR health risks caused by biofiltration and chlorination in water *** reduce potential BAR risk in drinking water,membrane filtration technology and water boiling are recommended at the point of use.

关键词： Drinking water treatment Antibiotic resistance gene Biofiltration Chlorination

Low nutrient levels as drinking water conditions can reduce the fitness cost of efflux pump-mediated ciprofloxacin resistance in Pseudomonas aeruginosa

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Journal of Environmental Sciences 2019年第9期31卷 123-132页

作者： wenfang lin Kun Wan Jie Zeng Jingjing Li Xi Li Xin Yu Key Lab of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China University of Chinese Academy of Sciences Beijing 100049 China

The long-term persistence of antibiotic resistance in the environment, especially in drinking water, is a public health concern. Expression of an efflux pump, an important mechanism of resistance to antibiotics, usually confers a fitness cost in bacteria. In this study, we aimed to determine why antibiotic resistance conferred by overexpression of an efflux pump persisted in low-nutrient environments(TOC < 10 mg/L) such as drinking and source water in which antibiotic selective pressure might be very low or even *** experiments between wild-type Pseudomonas aeruginosa and ciprofloxacinresistant mutants revealed that the fitness cost of ciprofloxacin resistance significantly decreased(p < 0.05) under low-nutrient(0.5 mg/L total organic carbon(TOC)) relative to high-nutrient(500 mg/L TOC) conditions. Mechanisms underlying this fitness cost were analyzed. The mexD gene expression in resistant bacteria(cip3 strain) was significantly lower(p < 0.05) in low-nutrient conditions, with 10 mg/L TOC((8.01 ± 0.82)-fold), than in high-nutrient conditions, with 500 mg/L TOC((48.89 ± 4.16)-fold). Moreover, rpoS gene expression in resistant bacteria((1.36 ± 0.13)-fold) was significantly lower(p < 0.05) than that in the wild-type strain((2.78 ± 0.29)-fold) under low-nutrient conditions(10 mg/L TOC),suggesting a growth advantage. Furthermore, the difference in metabolic activity between the two competing strains was significantly smaller(p < 0.05) in low-nutrient conditions(5 and 0.5 mg/L TOC). These results suggest that nutrient levels are a key factor in determining the persistence of antibiotic resistance conferred by efflux pumps in the natural environment with trace amounts or no antibiotics.

关键词： Efflux pumps Antibiotic resistance Fitness cost Low nutrient nfxB mutation Ciprofloxacin resistance

Molecular analysis of long-term biofilm formation on PVC and cast iron surfaces in drinking water distribution system

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Journal of Environmental Sciences 2014年第4期26卷 865-874页

作者： Ruyin Liu Junge Zhu Zhisheng Yu DevRaj Joshi Hongxun Zhang wenfang lin Min Yang College of Environmental and Resource Sciences University of Chinese Academy of Sciences Institute of Microbiology Chinese Academy of Sciences Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

To understand the impacts of different plumbing materials on long-term biofilm formation in water supply system, we analyzed microbial community compositions in the bulk water and biofilms on faucets with two different materials-polyvinyl chloride （PVC） and cast iron, which have been frequently used for more thanlO years. Pyrosequencing was employed to describe both bacterial and eukaryotic microbial compositions. Bacterial communities in the bulk water and biofilm samples were significantly different from each other. Specific bacterial populations colonized on the surface of different materials. Hyphomicrobia and corrosion associated bacteria, such as Acidithiobacillus spp., Aquabacterium spp., Limnobacter thiooxidans, and Thiocapsa spp., were the most dominant bacteria identified in the PVC and cast iron biofilms, respectively, suggesting that bacterial colonization on the material surfaces was selective. Mycobacteria and Legionella spp. were common potential pathogenic bacteria occurred in the biofilm samples, but their abundance was different in the two biofilm bacterial communities. In contrast, the biofilm samples showed more similar eukaryotic communities than the bulk water. Notably, potential pathogenic fungi, i.e., Aspergillus spp. and Candida parapsilosis, occurred in similar abundance in both biofilms. These results indicated that microbial community, especially bacterial composition was remarkably affected by the different pipe materials （PVC and cast iron）.

关键词： drinking waterbiofilm surface material pyrosequencing microbial community

Microbiological quality of roof tank water in an urban village in southeastern China

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Journal of Environmental Sciences 2023年第3期125卷 148-159页

作者： Dong Hu Jie Zeng Jing Chen wenfang lin Xinyan Xiao Mingbao Feng Xin Yu Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China Shitang Community Health Service Center Xiamen 361026China College of the Environment and Ecology Xiamen UniversityXiamen 361005China

Urban villages are unique residential neighborhoods in urban areas in China. Roof tanks are their main form of water supply, and water quality deterioration might occur in this system because of poor hygienic conditions and maintenance. In this study, water samples were seasonally collected from an urban village to investigate the influence of roof tanks as an additional water storage device on the variation in the microbial community structure and pathogenic gene markers. Water stagnation in the roof tank induced significant decreases in chlorine(p ling microbiological health risks in roof tanks in urban villages.

关键词： Urban village Water storage tank Microbiological water quality Propidium monoazide(PMA)-qPCR Illumina sequencing

WHIRLY1 recruits the histone deacetylase HDA15 repressing leaf senescence and flowering in Arabidopsis

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Journal of Integrative Plant Biology 2022年第7期64卷 1411-1429页

作者： Dongmei Huang Wei Lan Weibo Ma Rulin Huang wenfang lin Mengsi Li Chia-Yang Chen Keqiang Wu Ying Miao Fujian Provincial Key Laboratory of Plant Functional Biology College of Life SciencesFujian Agriculture and Forestry UniversityFuzhou 350002China Institute of Botany College of Life SciencesTaiwan UniversityTaibei 106China

Leaf senescence is controlled by a complex regulatory network in which robustness is ensured by the activity of transcription factors and epigenetic ***,how these coordinate the process of leaf senescence remains poorly *** found that WHIRLY1 interacts with Histone Deacetylase(HDA)15,a Reduced Potassium Dependence3(RPD3)/HDA1-type HDA,by using green fluorescent protein-nanotrap-mass spectrum *** development-dependent interaction between WHIRLY1 and HDA15 was further confirmed by bimolecular fluorescence complementation assays and co-immunoprecipitation assays in ***-omics genome-wide transcriptome and H3K9 acetylome enrichment analysis showed that HDA15 delays leaf senescence and flowering by repressing the expression of the positive regulators of leaf senescence and flowering,such as LOX2 and LARP1 C,and reducing H3K9 ac levels at these loci;WHIRLY1 and HDA15 co-target to the region near the transcription start site of a subset of nutrient recycling-related genes(e.g.,Glutathione S-transferases 10,non-coding RNA,and photosystem II protein D1 synthesizer attenuator PDIL1-2),as well as WRKY53 and ELF4,and co-repress their expression by removing H3K9 *** study revealed a key transcription regulatory node of nutrient recycling and senescence-associated genes involved in leaf senescence and flowering via the recruitment of HDA15 by the single-stranded DNA/RNA-binding protein WHIRLY1.

关键词： flowering HDA15 H3K9 acetylation leaf senescence multi-omics analysis WHY1

Localization shifting of WHIRLY1 leads to the change of JA,SA or ETH and Arabidopsis senescence and cell death

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Localization shifting of WHIRLY1 leads to the change of JA,S...

2013全国植物生物学大会

作者： lin wenfang REN Yujun MIAO Ying Center of Molecular Cell and Systems Biology College of Life ScienceFujian Agriculture and Forestry University

Plant endogenous hormones,growth environment and nutrition control plant development, formation and senescence *** more and more research were concentrated on the interaction or crosstalk of the several signaling pathways,however,their molecular mechanism are still less *** this study,a dual-localized protein WHIRLY1 shifting their localization between on plastid and the nucleus by developmental cues were investigated. Based on the dual localization of WHIRLY1 protein in the plastids and the nucleus,the partial mutants that harboured either a HA-tagged plastid-located isoform or a HA-tagged nucleus-located isoform were *** the changing of the localization of WHIRLY1 protein,the level of SA,JA and ETH were measured and the crosstalk network among SA,JA and ETH signal transduction pathways were summarized based on the ratio of SA and JA and ETH,and on the onset and processing of plant senescence and cell *** results of HPLC measurement of SA,JA and ETH showed that the level of endogenous SA and ETH decreased in overexpressing nuclear WHIRLY1 and increased in overexpressing plastid WHIRLY1 *** the level of endogenous JA shows an antagonistic *** overexpressing nuclear WHIRLY1 line shows stay-green phenotype,however,overexpressing plastid WHIRLY1 line enhances the senescent *** transcript level of WRKY53 and SAG12 declined in the overexpressing nuclear WHIRLY1 line but increased in the overexpressing plastid WHIRLY1 line, while the transcript level of plastid genes ndhF and psaB increased in overexpressing plastid WHIRLY1 line and decreased in knock out whyl *** will hypotheses that dual-located WHIRLY protein acting as an upstream regulator mediates the endogenous plant hormone and their corsstalk to regulate the nuclear events related to plant cell senescence and cell death.

关键词： Arabidopsis senescence dual-located protein WHIRLY1 JA SA ETH

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区域性偏好与城乡居民消费差异

统计研究 2009年第11期26卷 87-92页

作者：林文芳华中科技大学经济学院福建省统计局

本文利用2007年省域消费数据对城乡居民的消费偏好进行分析,结果表明省域、城乡居民各类消费存在着显著的差异和变化,城乡居民对各类商品消费具有明显的区域性偏好。城镇居民对食品、衣着、居住、医疗保健、交通和通信、文教娱乐消费存... 详细信息

本文利用2007年省域消费数据对城乡居民的消费偏好进行分析,结果表明省域、城乡居民各类消费存在着显著的差异和变化,城乡居民对各类商品消费具有明显的区域性偏好。城镇居民对食品、衣着、居住、医疗保健、交通和通信、文教娱乐消费存在省域间显著的相互影响,农村居民对食品、衣着、家庭设备用品及服务、医疗保健、交通和通信、文教娱乐的消费在省域间也具有明显的相互影响。同时,一些省份还形成局部的对分类商品消费具有区域性偏好的群体。

关键词：城乡居民消费消费差异区域性偏好

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县域城乡居民消费结构与收入关系分析

统计研究 2011年第4期28卷 49-56页

作者：林文芳华中科技大学福建省统计局

本文利用福建省县域居民消费样本调查数据,基于空间相关性和扩展线性支出系统,对我国县域居民消费结构与收入关系进行实证分析。结果表明:可支配(纯)收入对居民的各类消费具有显著的影响,而且对八类消费的作用强度不同;旨在提高居民收... 详细信息

本文利用福建省县域居民消费样本调查数据,基于空间相关性和扩展线性支出系统,对我国县域居民消费结构与收入关系进行实证分析。结果表明:可支配(纯)收入对居民的各类消费具有显著的影响,而且对八类消费的作用强度不同;旨在提高居民收入的收入分配制度改革能够促进消费需求,针对不同边际消费倾向的政策可以更有效地引导居民消费。本文的分析不同于以往对居民消费结构的研究在于:一是直接使用居民生活状况调查的微观数据进行ELES模型的参数估计,改变了仅使用七类收入群体数据的局限;二是在ELES模型中引入空间因素,考虑了因地理相邻对居民消费结构的影响,分析更为客观合理。

关键词：居民收入消费结构 ELES模型空间回归分析

H2O2 as a feedback signal on dual-localized WHIRLY1 affects leaf senescence in Arabidopsis

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H2O2 as a feedback signal on dual-localized WHIRLY1 affects ...

第三届全国植物开花·衰老与采后生物学大会

作者： wenfang lin Dongmei Huang Ban Deng Yujun Ren Ying Miao Fujian Provincial Key Laboratory of Plant Functional Biology College of Life Sciences Fujian Agriculture and Forestry University

Leaf senescence, either as a nature stage of development or an induced process underunfavorite conditions, incorporates multiple intricate signaling pathways. At the cellularlevel, retrograde signals have been considered as important players during the initiation and progression of senescence in both animals and plans. The plant-specific single-strand DNA-binding protein WHIRLY1(WHY1), a repressor of leaf nature senescence,is dually located in both nucleus and plastids. Despite many years of studies, however,the myth about its dual localizations and the underlying functional implication remainselusive. Here we provide further evidences in Arabidopsis that altering WHY1 allocation between the nucleus and chloroplast causes perturbation in HO homeostasis, resulting in adverse plant senescence phenotypes. Knockout of WHY1 increased H2 contents at 37 days post germination leading to an early leaf senescence phenotype, which can be rescued by expression of the nuclear isoform(nWHY1), but not by the plastid isoform(pWHY1). Targeted regulation on downstream genes, esp. two of the peroxidase genes PRX39 and PRX33, by the nucleus isoform nWHY1 is necessary for balancing the homeostasis of HO in the cell. On the other hand, exogenous HO treatment induced a substantial plastid accumulation of WHY1 proteins and at the same time reduced the nucleus isoforms. Loss of nucleus WHY1 isoform by HO treatment also accompanied with a global enhancement of both enrichments of H3 K9 ac and recruitment of RNAP II, particularly in the promoter of the senescence gene WRKY53, leading to an activated WRKY53 transcription and producing a senescence phenotype. Thus, distribution of WHY1 organelle isoforms and the feedback of HO intervene in a circularly integrated regulatory network during plant senescence in Arabidopsis.

关键词： WHIRLY1(WHY1) H2O2 plant senescence Arabidopsis thaliana

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连铸板坯热装炉对板材性能的影响

钢铁 1998年第11期33卷 19-22页

作者：肖寄光林文芳广东韶关钢铁集团有限公司

分析比较了Ｑ２３５－Ａ碳结钢及Ｂ级一般强度船板钢连铸板坯红坯“裸送”热装炉与冷装炉轧制成材的板材工艺与力学性能，热装炉工艺成材的冷弯及冲击性能明显低于后者。

关键词：连铸板坯热装炉轧制板材