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用改进的差别显示法克隆R6大鼠细胞的癌相关基因

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实验生物学报 2000年第3期33卷 199-206页

作者：陈晶郑坚瑜 YAMJ. HSIAOWl. 陈家童陈瑞阳南开大学分子生物学研究所天津300071 香港科技大学生物系

为了识别参与细胞癌变的基因,本文选择p53^(135-val)-过表达正常细胞系R6#13-8及其自发转化癌变细胞系T2作为研究材料,在克隆差异表达基因的同时,建立了一种简单、快速的差异表达基因分离的方法,并已克隆到2个与细胞癌变相关的候选新基... 详细信息

为了识别参与细胞癌变的基因,本文选择p53^(135-val)-过表达正常细胞系R6#13-8及其自发转化癌变细胞系T2作为研究材料,在克隆差异表达基因的同时,建立了一种简单、快速的差异表达基因分离的方法,并已克隆到2个与细胞癌变相关的候选新基因。本文所得的实验结果表明:这种方法实验步骤简单,避免使用同位素,RT-PCP扩增带的重复性好,能克隆到大于500bp的差异表达cDNA片段,差异表达的PCR cDNA片段假阳性率低,并可广泛适用于在两个或两个以上相对应真核细胞RNA群体中分离和克隆特异表达基因。研究结果还提示:在R6#13-8自发转化为T2的过程中涉及多个基因的激活与失活,这两个细胞系之间存在明显的基因表达差异。

关键词：差别显示法癌症 R6大鼠细胞 P53基因

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Adaptive evolution to the natural and anthropogenic environment in a global invasive crop pest,the cotton bollworm

Open Journal of Preventive Medicine（预防医学期刊） 2012年第3期

作者： wendy l. Ward Judith l. Weber Jeff Gossett Pippa Simpson Margaret l. Bogle James M. Robbins

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The Innovation 2023年第4期4卷 91-101页

作者： Minghui Jin Henry l.North Yan Peng Hangwei liu Bo liu Ruiqing Pan Yan Zhou Weigang Zheng Kaiyu liu Bo Yang lei Zhang Qi Xu Samia Elfekih wendy A.Valencia-Montoya Tom Walsh Peng Cui Yongfeng Zhou Kenneth Wilson Chris Jiggins Kongming Wu Yutao Xiao Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Gene Editing Technologies(Hainan)Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518116China The State Key laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesWest Yuanmingyuan RoadBeijing 100193China Department of Zoology University of CambridgeCambridge CB21SZUK College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China lancaster Environment Centre Lancaster UniversityLancaster LA14YWUK Berry Genomics Corporation Beijing 102200China Institute of Entomology School of Life SciencesCentral China Normal UniversityWuhan 430079China Australian Centre for Disease Preparedness(ACDP) CSIRO Health&BiosecurityEast GeelongVIC 3169Australia Bio21 Institute and the School of Biosciences The University of MelbourneMelbourneVIC 3010Australia Department of Organismic and Evolutionary Biology and Museum of Comparative Zoology Harvard UniversityCambridgeMA 02138USA CSIRO land and Water Black Mountain LaboratoriesCanberraACT 2601Australia

The cotton bollworm,Helicoverpa armigera,is set to become the most economically devastating crop pest in the world,threatening food security and biosafety as its range expands across the *** to understanding the eco-evolutionary dynamics of ***,and thus its management,is an understanding of population connectivity and the adaptations that allow the pest to establish in unique *** assembled a chromosome-scale reference genome and re-sequenced 503 individuals spanning the species range to delineate global patterns of connectivity,uncovering a previously cryptic population structure.

关键词： connectivity cotton evolution

Contrasting Historical and Recent Breakup Styles on the Meade River of Arctic Alaska in the Context of a Warming Climate

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American Journal of Climate Change 2013年第2期2卷 165-172页

作者： Richard A. Beck Kenneth M. Hinkel wendy R. Eisner Douglas Whiteman Christopher D. Arp Richard Machida Chris Cuomo Hongxing liu Changjoo Kim Andrew J. Rettig Chantal Ivenso Bo Yang Qiusheng Wu Haibin Su Shujie Wang Karen Frey John D. lenters Brittany l. Potter Atmospheric Radiation Measurement Program Atqasuk USA Department of Geography Clark University Worcester USA Department of Geography University of Cincinnati Cincinnati USA Department of GeogSchool of Natural Resources University of Nebraska at Lincoln Lincoln USA raphy Clark University Worcester USA Department of Philosophy University of Georgia Athens USA Department of Physics and Geosciences Texas A&M University-Kingsville Kingsville USA Information Technology University of Alaska at Fairbanks Fairbanks USA School of Natural Resources University of Nebraska at Lincoln Lincoln USA Water and Environmental Research Center University of Alaska Fairbanks USA

Although data for temporal spring river ice breakup are available for a number of Arctic rivers, there is a paucity of information related to the type of breakup. The Arctic Climate Impact Assessment (ACIA) of 2005 predicted a transition from mechanical to thermal spring breakup of ice cover on arctic rivers, with this shift being greatest in exclusively Arctic watersheds where observed warming is most pronounced. We describe a rare instance of an entirely Arctic river with limited but well documented historical and recent data regarding the type of breakup. Time-series ground imagery of spring breakup from 1966, 1975, 1978, 2009, 2010 and 2012, incombination with interviews of local inhabitants, documents a shift from predominantly mechanical to predominantly thermal breakup after spring 1978 and by spring 2009 within the context of a locally and regionally warming Arctic. The resultant shift from predominantly mechanical to predominantly thermal breakup is predicted to result in significant changes to water, sediment, nutrient and organic carbon fluxes, as well as riparian ecology and human activities.

关键词： Arctic Alaska Climate Change Meade River Thermal Breakup Mechanical Breakup Ice Snow

Diversity and host assemblage of avian haemosporidians in different terrestrial ecoregions of Peru

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Current Zoology 2022年第1期68卷 27-40页

作者： luz GARCIA-lONGORIA Jaime MURIEl Sergio MAGAllANES Zaira Hellen VIllA-GAlARCE leonila RICOPA Wilson Giancarlo INGA-DIAZ Esteban FONG Daniel VECCO Cesar GUERRA-SAlDANA Teresa SAlAS-RENGIFO wendy FlORES-SAAVEDRA Kathya ESPINOZA Carlos MENDOZA Blanca SAlDANA Manuel GONZAlEZ-BlAZQUEZ Henry GONZAlES-PINEDO Charlene lUJAN-VEGA Carlos Alberto Del AGUIlA Yessica VIlCA-HERRERA Carlos Alberto PINEDA Carmen REATEGUI Jorge Manuel CARDENAS-CAllIRGOS JoseAlberto IANNACONE Jorge luis MENDOZA Ravinder NMSEHGAl Alfonso MARZAl Departamento de Anatomia Biologia Celular y ZoologiaUniversidad de ExtremaduraBadajoz E-506071Spain Molecular Ecology and Evolution lab Department of BiologyLund UniversitySolvegatan 37SE-22362 LundSweden Instituto Pirenaico de Ecologıa-IPE(CSIC) Avda.Nuestra Senora de la Victoria 16Jaca 22700Spain DIRESA Direccion Regional de SaludLoreto 16001Peru Departamento Academico de Microbiologia y Parasitologia.Facultad de Ciencias Biologicas Universidad Nacional de la Amazonia PeruanaIquitos 16001Peru Facultad de Ciencias Agropecuarias Universidad Alas PeruanasLima 15823Peru EverGreen Institute-San Rafael Distrito de IndianaLoreto 16200Peru Observatorio de Aves loreto(lBO) Distrito de San JuanLoreto 16008Peru Centro Urku de Estudios Amazonicos Tarapoto 22200Peru Servicio Nacional de Areas Naturales Protegidas por el Estado 15036Peru Sanidad Animal-Facultad de Zootecnia Universidad Nacional Agraria La MolinaLima 15012Peru laboratorio de Microbiologia Facultad de Medicina Veterinaria y ZootecniaUniversidad CientUfica del SurLima 15067Peru laboratorio de Analisis Clinico Moraleslab SAC MoralesSan Martin 22201Peru Asociacion Ecosistemas Andinos(ECOAN) Cuzco 08002Peru Pharmacology and Toxicology Graduate Group University of CaliforniaDavisDA 95616USA Global Health Initiative Wabash CollegeCrawfordsvilleIN 47933USA Facultad de Ciencias Biologicas Universidad Nacional Mayor de San MarcosLima 15081Peru Facultad de Medicina Veterinaria Universidad Nacional Hermilio ValdizanHuanuco10160Peru Asociacion Peruana de Helmintologia e Invertebrados Afines Lima 15054Peru laboratorio de Ecologia y Biodiversidad Animal Universidad Nacional Federico VillarrealEl AgustinoLima 15007Peru laboratorio de Invertebrados Universidad Ricardo Palma-Santiago de SurcoLima 15537Peru Department of Biology San Francisco State UniversitySan FranciscoCA 94132USA

Characterizing the diversity and structure of host–parasite communities is crucial to understandingtheir eco-evolutionary *** and related haemosporidian parasites are responsible forfitness loss and mortality in bird species ***,despite exhibiting the greatest ornithologicalbiodiversity,avian haemosporidians from Neotropical regions are quite ***,we analyze the genetic diversity of bird haemosporidian parasites(Plasmodium andHaemoproteus)in 1,336 individuals belonging to 206 bird species to explore for differences in diversityof parasite lineages and bird species across 5 well-differentiated Peruvian *** 70 different haemosporidian lineages infecting 74 bird *** showed that 25 out ofthe 70 haplotypes had not been previously ***,we also identified 81 new host–parasite interactions representing new host records for these haemosporidian *** outcomesrevealed that the effective diversity(as well as the richness,abundance,and Shannon–Weaver index)for both birds and parasite lineages was higher in Amazon basin ***,we also showed that ecoregions with greater diversity of bird species also had highparasite richness,hence suggesting that host community is crucial in explaining parasite *** parasites were found in ecoregions with lower bird diversity,implying that the abundanceand richness of hosts may shape the exploitation strategy followed by haemosporidian *** outcomes reveal that Neotropical region is a major reservoir of unidentified *** studies analyzing host distribution and specificity of these parasites inthe tropics will provide important knowledge about phylogenetic relationships,phylogeography,and patterns of evolution and distribution of haemosporidian parasites.

关键词： Amazonia avian malaria generalist parasite habitat specificity Haemoproteus Plasmodium specialist parasite

Defining Stress among Corrections Professionals

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Dietary Cucumis melo Reduces Markers of Muscle and Articular Inflammation Following High-intensity Exercise in Horses

Open Journal of Preventive Medicine（预防医学期刊） 2021年第6期

作者： Jessica Ballin Meike Niederhausen Kerry S. Kuehl Diane l. Elliot wendy McGinnis Carol De Francesco

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Veterinary Science Research 2020年第1期2卷 24-34页

作者： Jennifer l.MacNicol Michael I.lindinger Anna K.Shoveller John P.Cant wendy Pearson Department of Animal Biosciences University of GuelphGuelphOntarioN1G 2W1Canada The Nutraceutical Alliance BurlingtonONCanada

We evaluated the antioxidative and anti-inflammatory potential of daily oral supplementation with a proprietary powdered Cucumis melo pulp(CMP)on exercise-induced markers of articular and muscular oxidative stress and inflammation in 12 *** performed a high-intensity exercise test immediately prior to,and then following,3 weeks of daily supplementation of 1 g powdered CMP(CMP;n=8).Controls(Co;n=8)underwent the same exercise and sampling regime but were not *** and synovial fluid(SF)samples were taken 24 h prior to exercise(Bl),and at 1 and 24 h following *** and SF were analysed for prostaglandin E2(PGE2),total antioxidant status(TAS),nitrite and superoxide dismutase(SOD)*** was analysed for glycosaminoglycans(GAG),and plasma was analysed for thiobarbituric acid reactive substances(TBARS).Comparisons were made using repeated measures with the initial exercise test as a *** was an increase in SF SOD activity in the CMP *** to Co at 1 h,CMP reduced nitrite and GAG in SF,as well as maintained plasma TAS and lymphocyte *** 24 h,plasma PGE2 and creatine kinase were lower in horses receiving *** weeks of supplementation with CMP reduced markers of articular and skeletal muscle oxidative stress and inflammation in response to high-intensity exercise in *** antioxidants may provide a useful adjunct to the daily nutrition plan of horses undergoing regular exercise training and competition.

关键词： Oxidative stress Inflammation Skeletal muscle Synovial fluid Antiinflammatory

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俯首于双神:结构弹性如何维持组织的混杂性

清华社会学评论 2021年第2期 182-234页

作者：温迪·史密斯玛丽亚·贝沙罗夫张政(译) 特拉华大学商业与经济学院康奈尔大学劳工关系学院南京大学社会学院

组织日益致力于应对混杂性,即身份、形式、逻辑或其他按照常规无法相配的核心要素的结合。本文利用柬埔寨一家成功的社会企业(数字鸿沟数据公司)前10年的纵向数据,归纳出一个通过结构弹性长期维持混杂性的基于实证的模型:稳定的组织特... 详细信息

组织日益致力于应对混杂性,即身份、形式、逻辑或其他按照常规无法相配的核心要素的结合。本文利用柬埔寨一家成功的社会企业(数字鸿沟数据公司)前10年的纵向数据,归纳出一个通过结构弹性长期维持混杂性的基于实证的模型:稳定的组织特征与适应性实施过程的相互作用。本文发现了两个稳定特征——悖论框架和护栏,前者涉及领导者对于混合型组织一体两面既相互矛盾又相互依赖的认知理解,后者由正式结构、领导才能和与混合体两面相联系的利益相关者关系组成。以上特征共同促进了混合体双重要素的意义和实践的持续适应,并维持其长期发展。本文的结构弹性模型将研究的重点从维持混杂性的稳定或适应路径转向理解二者间的相互作用,从而对于混杂性、二元性和适应性等方面的研究产生了更为广泛的影响。

关键词：组织身份悖论社会企业混合型组织

应用澳大利亚新西兰食品标准的营养素度量体系决定面向儿童“健康”食品营销的广告

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营养健康新观察 2014年第1期 39-40页

作者：温迪.沃森 Aimee Johnston Clare Hughes Kathy Chapman 刘轶群

目的探讨澳大利亚新西兰食品标准的营养素度量体系的潜在性,通过与其他既定的标准比较以支持限制面向儿童的"非健康食品"广告。方法将两周内悉尼三个电视频道中每日早6点至晚9点的食品广告分为通过和未通过营养素度量标准。关于能量、... 详细信息

目的探讨澳大利亚新西兰食品标准的营养素度量体系的潜在性,通过与其他既定的标准比较以支持限制面向儿童的"非健康食品"广告。方法将两周内悉尼三个电视频道中每日早6点至晚9点的食品广告分为通过和未通过营养素度量标准。关于能量、饱和脂肪、钠、糖、蛋白质、膳食纤维和水果,蔬菜,坚果和豆制品含量的营养素信息来源于食品标签、公司网站和制造商。分析结果与基于鉴定核心和非核心食品组标准和面向儿童自发食品行业规范的营销标准进行比较。结果与标准的基础确定和设置在自愿行业行为守则为向儿童推销公司的标准进行比较。结果在116个唯一的食品广告中,61%的未通过营养素度量标准的广告被确认为非健康食品。而在通过营养素度量标准的广告中,64%被认为是促进了核心食物,然而未通过营养素度量标准广告中的93%被认为促进了非核心的食物。在食品杂货的生产商公司行动计划范围内的63%符合营养素度量标准的广告通过了营养素度量法。只有一个快餐广告通过了快餐公司标准和营养素度量法。结论澳大利亚新西兰食品标准的营养素度量体系为产品的全面健康提供了一个独立的评估,并且在规范澳大利亚面向儿童的食品营销形成营养标准具有潜在可行性。目前的自主自我监管准则对于覆盖所有食品广告是宽松和失败的。

关键词：广告儿童食品营销营养素度量法规范