检索结果-南通市图书馆

Association between Polymorphisms of Glutathione S-Transferase and Progression to Cervical Cancer in Women from Burkina Faso and Mali

引用

Journal of Biosciences and Medicines 2020年第4期8卷 12-25页

作者： Teega-Wendé Clarisse Ouedraogo florencia wendkuuni djigma Théodora Mahoukèdè Zohoncon Boureima Idani Abdoul Karim Ouattara Pegdwendé Abel Sorgho Dorcas Obiri-Yeboah Prosper Bado Mah Alima Esther Traore Birama Diarra Albert Théophane Yonli Charlemagne Ouedraogo Jacques Simpore Laboratory of Molecular Biology and Genetics (LABIOGENE) University Joseph Ki-Zerbo Ouagadougou Burkina Faso Pietro Annigoni Biomolecular Research Centre (CERBA) Ouagadougou Burkina Faso University Saint Thomas d’Aquin Faculty of Medicine Ouagadougou Burkina Faso Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast PMB Cape Coast Ghana University Joseph Ki-Zerbo UFR/SDS Ouagadougou Burkina Faso

Although persistence of high-risk human papillomavirus infection is the main risk factor, Glutathione S-Transferase highly polymorphic enzyme involved in the metabolism of xenobiotics, is a good candidate gene. The objective of this study was to compare the polymorphisms of Glutathione S-Transferase M1-null in women with cancerous lesions and without lesions. This study consisted of 322 uterine cervix samples of women from Mali and Burkina Faso with Cervical Intra-epithelial Neoplasia 2 and 3, adenocarcinoma and squamous cell carcinoma and 100 women with no lesions. Human Papillomavirus genotyping was performed by Real-time multiplex Polymerase Chain Reaction. Glutathione S-Transferase gene polymorphisms were determined using conventional Polymerase Chain Reaction followed by migration on agarose gel. A statistically significant association with high relative risks of 10.77 for the development of High grade Superficial or Squamous Intra-epithelial Lesion (95% CI = 5.59 - 20.72;p < 0.001), and 13.20 for cancer development (95% CI = 6.79 - 25.63;p < 0.001) was found in women with the null genotype of Glutathione S-Transferase M1 in the study population. In Burkina Faso and Mali, Glutathione S-Transferase M1-null presented relative risks of 9 and 11.05 for high-grade lesions, 15 and 11.40 for cancer. Similarly, significant results had been observed in women with human papillomavirus positive and human papillomavirus negative. The results of the present study support the idea that the deletion of Glutathione S-Transferase M1 plays a crucial role in the progression of high-grade lesions and cervical cancer.

关键词： Glutathione S-Transferase M1-Null Cervical Cancer Burkina Faso Mali

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Role of Glutathione S-Transferase (<i>GSTM</i>1 and <i>GSTT</i>1) Genes Deletion in Susceptibility to HIV-1 Disease Progression

引用

Journal of Biosciences and Medicines 2020年第2期8卷 41-54页

作者： florencia wendkuuni djigma Pegdwendé Abel Sorgho Serge Théophile Soubeiga Albert Théophane Yonli Herman Karim Sombie Isabelle Touwendpoulimdé Kiendrebeogo Tégwindé Rebeca Compaore Abdoul Karim Ouattara Bapio V. J. T. Elvira Bazie Bolni Marius Nagalo Jacques Simpore Molecular Biology and Genetics Laboratory (LABIOGENE) Department of Biochemistry and Microbiology University JOSEPH KI-ZERBO Ouagadougou Burkina Faso Pietro Annigoni Biomolecular Research Center (CERBA) Ouagadougou Burkina Faso Institut de Recherche en Sciences de la Sante (IRSS) Biomedical and Public health Department Ouagadougou Burkina Faso Division of Hematology and Oncology Mayo Clinic Scottsdale AZ USA

Background: Glutathione S-transferases (GSTs) are multifunctional enzymes which play an important role in oxidative stress pathways by conjugation with glutathione. Oxidative stress is one of several risk factors that may be associated with many types of diseases progression such as cancer and infectious diseases. In this study, we investigated the association between the polymorphism of GSTM1 and GSTT1 genes and the risk of HIV-1 disease progression. Methods: We conducted a case-control study including 313 participants of Burkina Faso: 153 HIV-1 infected individuals on antiretroviral treatment (ART) and 160 HIV-1 negative individuals as controls. Presence or absence of the GSTM1 and GSTT1 genes was determined using multiplex polymerase chain reaction (PCR). CD4+ T counts and HIV-1 viral load were measured in patients using respectively BD FACSCount and Abbott m2000rt instruments. Results: Frequencies of GSTM1-null and GSTT1-null were 30.35% and 35.46% respectively and the frequency of double deletion GSTM1-null/GSTT1-null was 14.38% in the general study population. GSTM1-null (30.35% versus 69.65%;OR = 1.90;p = 0.010), GSTT1-null (35.46% versus 64.54%;OR = 3.11;p GSTM1-active/GSTT1-null (21.08% versus 48.56%;OR = 3.17;p GSTM1-null/GSTT1-null (14.38% versus 48.56%;OR = 4.46;p GSTM1-null and GSTM1-null/GSTT1-null were associated with increased odds of low CD4+ count (3) and high HIV-1 viral load (≥1000 copies/mL). Conclusion: GSTM1-null, GSTT1-null genotype, the double genotypes GSTM1-active/GSTT1-null and GSTM1-null/GSTT1-null were associated with HIV-1 disease progression and GSTM1-null and GSTM1- null/GSTT1-null genotypes were associated with low CD4+ T cells counts and high HIV-1 viral load in HIV-1infected patients on ART.

关键词： HIV-1 GSTM1 GSTT1 Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Diagnosis of Sexual Differentiation Disorders in Burkina Faso

引用

Journal of Biosciences and Medicines 2024年第3期12卷 1-15页

作者： Bazie Bapio Valérie Elvira Jean Télesphore Sawadogo Soumaïla Zohoncon Théodora Mahoukèdè Tall Ahmadou Bado Prosper Soubeiga Serge Théophile Ouattara Moussa Sorgho Abel Kiendrebeogo Touwendpoulimdé Isabelle Yonli Albert Theophane djigma florencia Millogo/Traore Timongo Françoise Danielle Simpore Jacques Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Université Joseph KI-ZERBO Unité de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigonie (CERBA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Régional de Ouahigouya Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Sex development anomalies represent a group of congenital pathologies in which chromosomal, gonadal and anatomical sex differentiation is atypical. The aim of our study was to use molecular biology techniques to elucidate sex in cases of anomalies of sexual differentiation in Burkina Faso. This cross-sectional study took place from March 2023 to June 2023. Oral and blood samples were collected respectively using sterile swabs and stored on Swab Kits and NUCLEIcardTM (https://***/product-ranges/nucleic-card/) were used to determine gonosome profiles. Extraction was carried out using the DNA Swap solution Kit and the DNA IQ System Kit, and a PowerPlex? 21 kit (Promega) for amplification. The Applied Biosystems 9700 thermal cycler was used for PCR followed by 36 cm capillary electrophoresis in the Applied Biosystems 3130 prism sequencer. Sequence files were analyzed using GeneMapper IDX v. 3.2 software. Seven (07) patients were registered during the study period. There were 4 cases of XX DSD or 46, XX DSD and 3 cases of XY DSD or 46, XY DSD. The median age of our patients was 16 years. Civilian sex was male in 4 cases and female in 3. The most frequent reason for consultation was micropenis in 3 cases, followed by primary amenorrhea and sex ambiguity. There were 03 cases of discrepancy between genetic sex and civil sex. The accessibility of molecular diagnosis is little known to clinicians. XX DSDs or 46, XX DSDs were the most frequent (4/7) in our study. The problem facing this situation is early diagnosis to help prevent complications in Burkina Faso.

关键词： Gonosome DSD Abnormal Sexual Development Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Efficiency of HAART in the prevention of mother to children HIV-1 transmission at Saint Camille medical centre in Burkina Faso,West Africa

引用

Asian Pacific Journal of Tropical Medicine 2012年第12期5卷 991-994页

作者： Laure Stella Ghoma Linguissi Cyrille Bisseye Tani Sagna Bolni Marius Nagalo Djeneba Ouermi florencia W djigma Salvatore Pignatelli Joseph D Sia Virginio Pietra Remy Moret Jean Baptiste Nikiema Jacques Simpore Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA)/LABIOGENE UFR/SVTUniversite de OuagadougouBurkina Faso Department de Biologie Unirersite des Sciences et Techniques de Masuku(USTM) FrancerilleGabon Centre Medical Saint Camille OuagadougouBurkina Faso

Objective:To evaluate efficiency of HAART in the prevention of mother to child HIV ***:A longitudinal study was conducted on 1 300 women attending the antenatal service at Saint Camille Medical Centre from September 2010 to July *** HIV status of mothers was determined by rapid tests and *** results were confirmed by real-time *** was used to determine HIV status of children born from HIV-positive ***:Among 1 300 pregnant women tested for HIV,378 were *** were predominantly housewives(69.7%),and their mean age was(28.32±0.15) *** overall prevalence of HIV transmission from mother to child was 4.8%(18/378).This prevalence differed significantly from 0.0%(0/114) to 6.8%(18/264) in children born from mothers under HAART and those with mothers under New Prophylactic Protocol(ACT + 3TC + NVP),respectively(P< 0.01).Children’s mortality rate during the medical follow up was 1.3%(5/378).Among 16 women with HIV dubious status by ELISA,the Real Time PCR confirmed 2/16(12.5%) as HIV positive. Conclusions:The protocol of prevention of mother to children HIV transmission(PMTCT) is *** rate of HIV vertical transmission is significantly *** diagnosis determined by PCR of children born from HIV- positive mother is necessary and recommended in the context of PMTCT in Burkina *** also found that PCR is an effective tool to confirm HIV status in pregnant women.

关键词： Pregnant Women Child HIV PCR DNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antimicrobial resistance of abnormal vaginal discharges microorganisms in Ouagadougou,Burkina Faso

引用

Asian Pacific Journal of Tropical Biomedicine 2012年第4期2卷 294-297页

作者： Simplice D Karou florencia djigma Tani Sagna Christelle Nadembega Moctar Zeba Aboudoulaye Kabre Kokou Anani Djeneba Ouermi Charlemagne Gnoula Virginio Pietra Salvatore Pignatelli Jacques Simpore Centre de Recherche Biomoleculaire Pietro Annigoni(CERBA) Ecole Supericure des Techniques Alimentaires et Biologiques(ESTBA-UL) Université de Lomé Centre Medical Saint Camille de Ouagadougou

Objective:To assess the prevalence of bacterial strains and fungal strains infecting the vaginal tract and test their sensitivity to antibiotics in women attending Saint Camille Medical Centre in ***:From January 2008 to December 2009,a total of 2000 vaginal swabs were cultivated for bacterial and fungal identification and ***,bacterial strains were tested for their susceptibility to several antibiotics used in routine in the centre. Results:The results revealed that microbial isolation and identification was attempted for 1536/2000 sample,a positivity rate of 76.80%.Candida albicans(48.76%),followed by Escherichia coli(16.67%),Streptococcus agalactiae(8-14%) and Staphylococcus aureus(7.55%) were the major agents of genital tract infections in *** hominis and Ureaplasma urealyticum combined accounted for less than 7%.Trichomonas vaginalis was identified in 1.04%*** antimicrobial tests revealed that the microorganisms developed resistance to several antibiotics including beta ***,antibiotics such as cefamenzol,ciprofloxacin and norfloxacin were still active on these ***:The results reveal that many sexually active women are infected by one or more microbial pathogens,probably because of the lock of hygiene or the adoption of some risky behaviors,such as not using condoms or having multiple sexual *** should be made to address these points in the country.

关键词： Bacteria Genital infections Antibiotics Mycoplasma Fungal strain Antimicrobial resistance Abnormal vaginal discharge Vaginal infection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Glucose-6-phosphate dehydrogenase(G6PD) deficiency is associated with asymptomatic malaria in a rural community in Burkina Faso

引用

Asian Pacific Journal of Tropical Biomedicine 2014年第8期4卷 655-658页

作者： Abdoul Karim Ouattara Cyrille Bisseye Bapio Valery Jean Télesphore Elvira Bazie Birama Diarra Tegwindé Rebeca Compaore florencia djigma Virginio Pietra Remy Moret Jacques Simpore Centre for Biomolecular Research Pietro Annigoni(CERBA) LABIOGENE UFR/SVT University of Ouagadougou BP 364 OuagadougouBurkina Faso Laboratory of Molecular and Cellular Biology(LABMC) University of Science and Technology of Masuku(USTM)BP 943 FrancerilleGabon

Objective:To investigate 4 combinations of mutations responsible for glucose-6—phosphate dehydrogenase(G6PD) deficiency in a rural community of Burkina Faso,a malaria endemic ***:Two hundred individuals in a rural community were genotyped for the mutations A376 G.G202A,A542 T,G680T and T968 C using TaqMan single nucleotide polymorphism assays and polymerase chain reaction followed by restriction fragment length ***:The prevalence of the G6 PD deficiency was 9.5%,in the study *** was significantly higher in men compared to women(14.23%vs 6.0%,P=0.049).The 202A/376 G G6PD Awas the only deficient variant *** falciparum asymptomatic parasitemia was significantly higher among the C6PD-non—deficient persons compared to the G6PD-deficient(P<0.001).The asymptomatic parasitemia was also significantly higher among G(SPI) nondeficient compared to C6PD—heterozygous females(P<0.001).Conclusions:This study showed that the G6 PD A- variant associated with protection against asymptomatic malaria in Burkina Faso is probably the most common deficient variant.

关键词： Polymerase chain reaction Mutations Glucose-6-phosphate dehydrogenase deficiency Asymptomatic malaria Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

引用

American Journal of Molecular Biology 2023年第4期13卷 276-290页

作者： Théodora Mahoukèdè Zohoncon Shoukrat Ohuwa Toyin Bello Prosper Bado Rogomenoma Alice Ouédraogo Estelle Ouédraogo Ina Marie Angèle Traoré Abdoul Karim Ouattara florencia Wenkunni djigma Albert Théophane Yonli Assita Sanou-Lamien Olga Mélanie Lompo Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Centre Hospitalo-Universitaire Yalgado Ouedraogo Ouagadougou Burkina Faso Pathological Anatomy and Cytology Laboratory Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Introduction: Cervical cancer, caused by persistent high-risk human papillomavirus (HPV) infection, remains a global public health problem. The cellular transformation and maintenance of the malignant phenotype of these HPVs are attributed to the viral oncoproteins E6 and E7. Objective: This study aims to detect the presence of human papillomavirus DNA and E6/E7 oncoprotein mRNA of HPV genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous lesions, histologically confirmed in Burkina Faso. Methods: This descriptive cross-sectional study focused on cases of cervical cancer and high-grade intraepithelial neoplasia (CIN) and was conducted from June to December 2022. One hundred (100) samples of fixed and paraffin-embedded tissues were collected from the pathological anatomy and cytology laboratories of hospitals in the capital of Burkina Faso. High-risk human papillomavirus (HR-HPV) DNA was detected using multiplex real-time PCR, while the presence of E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined using real-time Reverse Transcriptase-PCR (RT-PCR) with TaqMan probes. Results: The mean age of women diagnosed with cervical cancer and high-grade CIN was 50.81 ± 13.65 years, ranging from 22 to 82 years. Cervical cancer and high-grade CIN were positive for at least one high-risk human papillomavirus (HR-HPV) in 80% of cases. The most prevalent genotypes observed were HPV16, 18, 31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%;95% CI: [94.58 - 99.94]) were also positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16, 18, 31, and 33. Conclusion: In the presence of HPV DNA, testing for E6 and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool for improved assessment of the progression to cervical cancer.

关键词： HPV E6/E7 Cervical Cancer Precancerous Lesions Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gene therapy for Parkinson’s Disease and Ethical Challenges: A Systematic Review

引用

Advances in Parkinson's Disease 2023年第2期12卷 9-28页

作者： Théodora M. Zohoncon Joseph Sawadogo Abdou Azaque Zoure Abdoul Karim Ouattara Marie N. L. Ouedraogo Luc Zongo Paul Ouedraogo florencia W. djigma Christelle W. M. Nadembèga Raphael Kabore Djénéba Ouermi Dorcas Obiri-Yeboah Jacques Simpore Pietro Annigoni Biomolecular Research Centre (CERBA) Ouagadougou Burkina Faso Faculty of Medicine University Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Saint Camille Hospital Ouagadougou (HOSCO) Ouagadougou Burkina Faso Molecular Biology and Genetics Laboratory (LABIOGENE) Joseph KI-ZERBO University Ouagadougou Burkina Faso National Center for Scientific and Technological Research (CNRST/IRSS) Ouagadougou Burkina Faso Norbert Zongo University/Manga University Centre Koudougou Burkina Faso Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana

Background: Parkinson’s disease (PD) is a complex, multifactorial neurodegenerative disorder with a pathophysiology deriving from the synergy of abnormal aggregation of neuroinflammation, synuclein and dysfunction of lysosomes, mitochondria and synaptic transport difficulties influenced by genetic and idiopathic factors. Worldwide, PD has a prevalence of 2-3% in people over the age of 65. To date, there is no certified, effective treatment for PD. Aim: The aims of this research were: (i) to present, on the basis of recent advances in molecular genetics and epigenetics, the genomic aspects and challenges of gene therapy trials for PD;(ii) to outline the ethical principles applicable to therapeutic trials for PD. Method: A systematic literature review was carried out to identify relevant articles reporting on genomic aspects and gene therapy in PD from 2001 to October 2023. The search was conducted in French and/or English in three databases: PubMed, Google Scholar and Science Direct. PRISMA guidelines were used in this systematic review. Results: A total of thirty-three publications were selected. An inductive thematic analysis revealed that numerous genetic mutations (SNCA, Parkin, PINK1, DJ-1, LRRK2, ATP13A2, VPS35, Parkin/PRKN, PINK1, DJ1/PARK7) and epigenetic events such as the action of certain miRNAs (miR-7, miR-153, miR-133b, miR-124, miR-137) are responsible for the onset of PD, and that genetic therapy for this pathology raises ethical questions that need to be elucidated in the light of the bioethical principles of autonomy, beneficence, non-maleficence and justice. Conclusion: There is no zero risk in biotechnology. Then, it will be necessary to assess all the potential risks of Parkinson disease’s gene therapy to make the right decision. It is therefore essential to pursue research and, with the guidance of ethics, to advance treatment options and meet the challenges of brain manipulation and its impact on human identity. The golden rule of medicine remai

关键词： Neurodegenerative Diseases Parkinson Disease Molecular Mechanism Gene Therapy Gene Therapy Ethics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Presymptomatic Diagnosis and Gene Therapy for Alzheimer’s Disease: Genomic, Therapeutic, and Ethical Aspects—A Systematic Review

引用

Advances in Alzheimer's Disease 2023年第4期12卷 55-74页

作者： Théodora M. Zohoncon Joseph Sawadogo Abdoul Karim Ouattara Abdou Azaque Zoure Marie N. L. Ouedraogo Paul Ouedraogo florencia W. djigma Christelle W. M. Nadembèga Raphael Kabore Djénéba Ouermi Dorcas Obiri-Yeboah Jacques Simpore Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculty of Medicine University Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Laboratoire de Biologie Moléculaire H&#244pital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouaga Burkina Département de Biochimie microbiologie Université Norbert Zongo/Centre Universitaire de Manga Koudougou Burkina Faso Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana

Over the past three decades, genomic and epigenetic sciences have identified more than 70 genes involved in the molecular pathophysiology of Alzheimer’s disease (AD). DNA methylation, abnormal histone and chromatin regulation and the action of various miRNAs induce AD. The identification of mutated genes has paved the way for the development of diagnostic kits and the initiation of gene therapy trials. However, despite major advances in neuroscience research, there is yet no suitable treatment for AD. Therefore, the early diagnosis of this neurodegenerative disease raises several ethical questions, including the balance between the principle of non-maleficence and the principle of beneficence. The aims of this research were to present the genomic and ethical aspects of AD, and to highlight the ethical principles involved in its presymptomatic diagnosis and therapy. A systematic review of the literature in PubMed, Google Scholar and Science Direct was carried out to outline the genomic aspects and ethical principles relating not only to the presymptomatic diagnosis of AD, but also to its gene therapy. A total of 16 publications were selected. AD is a multifactorial disease that can be genetically classified into Sporadic Alzheimer’s Disease and Familial Alzheimer’s Disease based on family history. Gene therapy targeting specific disease-causing genes is a promising therapeutic strategy. Advancements in artificial intelligence applications may enable the prediction of AD onset several years in advance. While early diagnosis of AD may empower patients with full decision competence for early decision-making, it also carries implications for the patient’s family members, who are at risk of developing the disease, potentially becoming a source of confusion or anxiety. AD has a significant impact on the life of individuals at risk and their families. Given the absence of disease modifying therapy, genetic screening and early diagnosis for this condition raise ethical issue

关键词： Neurodegenerative Diseases Alzheimer’s Disease Molecular Mechanism Gene Therapy Presymptomatic Diagnosis Ethics Gene Therapy Ethics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

引用

Journal of Biosciences and Medicines 2023年第9期11卷 171-180页

作者： Ali Kandé Maïmouna Ilboudo Djénéba Ouermi Ina Marie Angèle Traore Abdoul Karim Ouattara Théodora Mahoukèdè Zohoncon Esther Mah Alima Traore florencia W. djigma Dorcas Obiri-Yeboah Henriette Poaty Yvette Marie Chantal Gyebre Olga M. Lompo Charlemagne MR Ouedraogo Jacques Simpore Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Laboratoire de Biologie moléculaire et de génétique (LABIOGENE) UFR/SVT Université Joseph Ki-Zerbo Ouagadougou Burkina Faso University Post Office Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. These viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV ENT infections can lead to benign or malignant tumors in which we could find both LR-HPV and HR-HPV genotypes. The objective of this study was to investigate the genotypes of HR-HPV and LR-HPV in archived tissue samples derived from both benign and malignant tumors of the ear, nose, and throat (ENT) in Ouagadougou, Burkina Faso. One hundred and twenty formalin-fixed, paraffin-embedded archived tissues of the ENT sphere from 26 benign tumors and 94 malignant tumors were included. The tissues were first deparaffinized with xylem. The extracted DNA was used to test for high-risk and low-risk HPV by Real-Time Multiplex PCR. HPV DNA was found in 57.7% (15/26) of benign tumors and 43.61% (41/94) of malignant tumors. The prevalence of HPV infection was 46.67% (56/120) in all tumors combined. The most common HPV genotypes found were HPV 11 (34.28%), HPV 6 (30%), HPV56 (14.28%) and HPV 33 (8.57%). There were 21.43% (12/56) cases of genotypes co-infections with 10 cases of double infection and 2 cases of triple infection. Both low-risk and high-risk HPV are found in ENT tumors with relatively high HPV prevalence.

关键词： HPV Archived Tissues ENT Tumor Burkina Faso

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：