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Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

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Journal of Integrative Plant Biology 2015年第7期57卷 641-652页

作者： Fen Lu Huiqin Wang Shanzhi Wang wendi jiang Changlin Shan Bin Li Jun Yang Shiyong Zhang Wenxian Sun Department of Plant Pathology China Agricultural University Key Laboratory in Plant Pathology Ministry of AgricultureChina Agricultural University State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of AgricultureInstitute of BiotechnologyZhejiang University Rice Research Institute Shandong Academy of Agricultural Science

The elongation factor Tu （EF-Tu） receptor （EFR） in cruciferous plants specifically recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants （Oryza sativa L. ssp. japonica cv. Zhonghua 17） and cell cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We＇demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa- tion in the AtEFR transgenic rice cells and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatically in transgenic rice plant and cell lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig- gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expressionlevel. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Collectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

关键词： Bacterial disease resistance elongation factor Tu receptor interspecific gene transfer pattern recognition receptor rice

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论现代语文教育对学生语言经验的偏离

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武夷学院学报 2009年第1期28卷 108-111页

作者：蒋文棣广东培正学院人文学科与基础教学部广东广州510830

语言经验是个人从学习到使用语言的过程中对语言的体验和积累。语文教育过程是语言经验的"改造和重组",而不仅是知识的记忆,语文教育的目标在于以较高的效率促进这一过程。然而现代语文教育在材料上脱离学生的语言经验和社会生活,教学... 详细信息

语言经验是个人从学习到使用语言的过程中对语言的体验和积累。语文教育过程是语言经验的"改造和重组",而不仅是知识的记忆,语文教育的目标在于以较高的效率促进这一过程。然而现代语文教育在材料上脱离学生的语言经验和社会生活,教学不以学生为中心,脱离了以语言能力为核心的语文教育的本意。

关键词：语言经验语文教育语言能力

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P4HA2通过激活PI3K/AKT/mTOR信号通路促进肝癌的发生和发展

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南方医科大学学报 2022年第5期42卷 665-672页

作者：商玲姜雯迪张俊丽武文娟蚌埠医学院肿瘤基础研究与临床检验诊断重点实验室安徽蚌埠233030 蚌埠医学院检验医学院生物化学与分子生物学教研室安徽蚌埠233030

目的探讨脯氨酸4-羟化酶Ⅱ(P4HA2)在肝癌细胞发生发展中的作用及相关机制。方法利用GEPIA、Human Protein Atlas数据库预测P4HA2在肝癌中的表达情况,利用K-M plotter在线数据库分析P4HA2的表达情况与肝癌预后的关系,采用qRT-PCR和West... 详细信息

目的探讨脯氨酸4-羟化酶Ⅱ(P4HA2)在肝癌细胞发生发展中的作用及相关机制。方法利用GEPIA、Human Protein Atlas数据库预测P4HA2在肝癌中的表达情况,利用K-M plotter在线数据库分析P4HA2的表达情况与肝癌预后的关系,采用qRT-PCR和Western blot检测肝癌细胞和正常肝细胞中P4HA2的表达。构建慢病毒载体,用携带P4HA2 shRNA和Con shRNA的慢病毒载体分别转染肝癌SNU-449和Hep-3B细胞系,建立沉默表达P4HA2的细胞株(shP4HA2组)和对照组细胞株(NC组)。采用CCK-8、集落形成试验、划痕实验和Transwell实验分别检测细胞增殖、迁移和侵袭能力。采用Western blot实验检测上皮-间质转化和PI3K/Akt/mTOR信号通路相关蛋白表达情况。结果在线数据库分析结果显示,肝癌组织中P4HA2表达高于正常肝组织(P<0.05)。同时,肝癌细胞系中P4HA2 mRNA和蛋白表达水平也高于正常肝细胞(P<0.01)。慢病毒干扰后,与NC组相比,shP4HA2组中mRNA和蛋白表达水平下降(P<0.05)。P4HA2基因表达沉默后,细胞的增殖、迁移和侵袭受到抑制。Western blot显示,相对于NC组,shP4HA2组的E-cadherin蛋白表达上升,N-cadherin、Snail蛋白表达下降(P<0.05),在PI3K/AKT/mTOR通路中,磷酸化的PI3K(P-PI3K)、AKT(P-AKT)和m TOR(P-mTOR)显示出较低的水平(P<0.05)。结论 P4HA2通过激活PI3K/Akt/mTOR信号通路影响肝癌细胞的增殖、迁移、侵袭,促进肝癌的发生发展。

关键词：肝癌脯氨酸4-羟化酶II 迁移侵袭 PI3K/AKT/mTOR

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Impacts of the annual cycle of background SST in the tropical Pacific on the phase and amplitude of ENSO

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 12-17页

作者： Song jiang Congwen Zhu Ning jiang State Key Laboratory of Severe Weather(LASW) Chinese Academy of Meteorological SciencesBeijingChina College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijingChina

The dominant annual cycle of sea surface temperature(SST)in the tropical Pacific exhibits an antisymmetric mode,which explains 83.4%total variance,and serves as a background of El Niño-Southern Oscillation(ENSO).However,there is no consensus yet on its anomalous impacts on the phase and amplitude of *** on data during 1982-2022,results show that anomalies of the antisymmetric mode can affect the evolution of ENSO on the interannual scale via Bjerknes feedback,in which the positive(negative)phase of the antisymmetric mode can strengthen El Niño(La Niña)in boreal winter via an earlier(delayed)seasonal cycle transition and larger(smaller)annual *** magnitude of the SST anomalies in the equatorial eastern Pacific can reach more than±0.3◦C,regulated by the changes in the antisymmetric mode based on random sensitivity *** reveal the spatial pattern of the annual cycle associated with the seasonal phase-locking of ENSO evolution and provide new insight into the impact of the annual cycle of background SST on ENSO,which possibly carries important implications for forecasting ENSO.

关键词： Annual cycle SST anomaly Antisymmetric mode ENSO

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Effects of carbon content on the microstructure and tensile properties of a low-density steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 391-401页

作者： Yongxuan Shang Mingyu Fan Shuyong jiang Zhongwu Zhang Key Laboratory of Superlight Materials and Surface Technology Ministry of EducationCollege of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001China State Key Laboratory of Metal Material for Marine Equipment and Application Iron&Steel Research Institute of Ansteel Group CorporationAnshan 114009China

Carbon can change the phase components of low-density steels and influence the mechanical *** this study,a new method to control the carbon content and avoid the formation ofδ-ferrite by decarburization treatment was *** microstructural changes and mechanical characteristics with carbon content induced by decarburization were systematically ***-Jaoul(C-J)analysis was employed to examine the work hardening characteristics during the tensile *** decarburization by heat treatments,the carbon content within the austenite phase decreased,while Mn and Al were almost unchanged;this made the steel with full austenite transform into the austenite and ferrite dual ***,(Ti,V)C carbides existed in both matrix phase and the mole fraction almost the *** addition,the formation of other carbides *** loss induced a decrease in strength due to the weakening of the carbon solid *** the steel with the single austinite,the deformation mode of austenite was the dislocation planar glide,resulting in the formation of *** the dual-phase steel,the deformation occurred by the dislocation planar glide of austenite first,with the increase in strain,the cross slip of ferrite took place,forming dislocation cells in *** the late stage of deformation,the work hardening of austinite increased rapidly,while that of ferrite increased slightly.

关键词： low-density steels carbon content decarburization strengthening mechanisms work hardening behavior

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Effect of lamellarization on the microstructure and mechanical properties of marine 10Ni5CrMoV steel

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 402-413页

作者： Tao Zou Yanwu Dong Zhouhua jiang Shuyang Du Yushuo Li School of Metallurgy Northeastern UniversityShenyang 110819China Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110819China Key Laboratory for Ecological Metallurgy of Multimetallic Ores(Ministry of Education) Northeastern UniversityShenyang 110819China

Multistage heat treatment involving quenching(Q),lamellarizing(L),and tempering(T)is applied to marine 10Ni5CrMoV *** microstructure and mechanical properties were studied by multiscale characterizations,and the kinetics of reverse austenite transformation,strain hardening behavior,and toughening mechanism were further *** lamellarized specimens possess low yield strength but high toughness,especially cryogenic *** leads to the development of film-like reversed austenite at the martensite block and lath boundaries,refining the martensite structure and lowering the equivalent grain *** analysis of austenite reversion based on the JMAK model shows that the isothermal transformation is dominated by the growth of reversed austenite,and the maximum transformation of reversed austenite is reached at the peak temperature(750℃).The strain hardening behavior based on the modified Crussard-Jaoul analysis indicates that the reversed austenite obtained from lamellarization reduces the proportion of martensite,significantly hindering crack propagation via martensitic transformation during the *** a consequence,the QLT specimens exhibit high machinability and low yield *** with the QT specimen,the ductile-brittle transition temperature of the QLT specimens decreases from-116 to-130℃due to the low equivalent grain size and reversed austenite,which increases the cleavage force required for crack propagation and absorbs the energy of external load,*** work provides an idea to improve the cryogenic toughness of marine 10Ni5CrMoV steel and lays a theoretical foundation for its industrial application and comprehensive performance improvement.

关键词： 10Ni5CrMoV steel lamellarizing reversed austenite cryogenic toughness ductile-brittle transition temperature

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颈动脉溃疡斑块与缺血性脑卒中发生的关系

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血管与腔内血管外科杂志 2024年第1期10卷 113-116页

作者：张会单任冰邹博姜文弟北京市仁和医院超声科北京102600

目的探讨颈动脉溃疡斑块与缺血性脑卒中(IS)发生的关系。方法收集2018年6月至2020年12月于北京市仁和医院就诊的38例缺血性脑血管疾病患者的临床资料,所有患者均接受颈动脉彩色多普勒超声检查(CDU)及头颅磁共振成像(MRI)检查,分析患者... 详细信息

目的探讨颈动脉溃疡斑块与缺血性脑卒中(IS)发生的关系。方法收集2018年6月至2020年12月于北京市仁和医院就诊的38例缺血性脑血管疾病患者的临床资料,所有患者均接受颈动脉彩色多普勒超声检查(CDU)及头颅磁共振成像(MRI)检查,分析患者是否存在颈动脉溃疡斑及其与IS发生的关系。结果38例缺血性脑血管病患者共发现颈动脉溃疡斑块40个,其中,低回声斑块27个(67.50%),低回声为主的混合回声斑块13个(32.50%)。29例患者确诊为IS,阳性率为76.31%(29/38),其中,低回声斑块21个(72.41%),低回声为主的混合回声斑块8个(27.59%)。结论颈动脉溃疡斑块与IS发生关系密切,低回声溃疡斑块发生率高于低回声为主的混合回声斑块,低回声溃疡斑IS发生率高于低回声为主的混合回声斑块。

关键词：颈动脉彩色多普勒超声溃疡斑块缺血性脑卒中

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Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： Song He Li Li Sai Wang Shao-jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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方舱医院新型冠状病毒Omicron防控工作人员正确穿、脱个人防护装备的认知和技能现况调查

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中国全科医学 2022年第30期25卷 3726-3733页

作者：姜文迪王金金李珊珊杨洋郝帅童智慧蔡文鹏南京大学医学院附属金陵医院普通外科江苏省南京市210018 南京大学医学院附属金陵医院护理部江苏省南京市210018 南京大学医学院附属金陵医院心血管内科江苏省南京市210018 南京大学医学院附属金陵医院重症医学科江苏省南京市210018 海军军医大学心理系医学心理学教研室上海市200433

背景新型冠状病毒(SARS-CoV-2)Omicron变异株传播速度增快、隐匿性增强,方舱医院SARS-CoV-2防控工作人员正确穿、脱个人防护装备(PPE)技能不仅直接影响入舱人员自身健康,还关乎方舱医院整体感染控制质量。目的分析方舱医院SARS-CoV-2防... 详细信息

背景新型冠状病毒(SARS-CoV-2)Omicron变异株传播速度增快、隐匿性增强,方舱医院SARS-CoV-2防控工作人员正确穿、脱个人防护装备(PPE)技能不仅直接影响入舱人员自身健康,还关乎方舱医院整体感染控制质量。目的分析方舱医院SARS-CoV-2防控工作人员对正确穿、脱PPE的认知和技能。方法采用整群抽样方法选取2022-04-10至2022-05-10进入国家会展中心(上海)方舱医院第四分院的SARS-CoV-2防控工作人员460名,通过自制网络调查问卷评估其对正确穿、脱PPE的认知,通过现场考核评估其正确穿、脱PPE的技能。结果共发放网络调查问卷460份,回收有效问卷384份,回收有效率为83.5%;对于《正确穿、脱PPE认知评价量表》评分,医生、护士第一次更衣区、第二次更衣区、前置脱衣区和第一次脱衣区、第二次脱衣区、手卫生消毒维度评分高于公安和消防人员(P<0.05)。460名方舱医院SARS-CoV-2防控工作人员均完成正确穿、脱PPE技能现场考核;对于《正确穿、脱PPE技能考核表》评分,医生、护士第一次更衣区、清洁区入污染区更衣步骤维度评分低于公安和消防人员,医生第一次更衣区维度评分低于护士(P<0.05)。结论不同岗位的方舱医院SARS-CoV-2防控工作人员对正确穿、脱PPE的认知和技能掌握程度存在一定差异,且对穿、脱PPE认知和技能存在不匹配的情况,应注意开展有针对性、有重点的培训和督导。

关键词：新型冠状病毒肺炎新型冠状病毒个人防护装备方舱医院认知横断面研究

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Toward understanding the role of genomic repeat elements in neurodegenerative diseases

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Neural Regeneration Research 2025年第3期20卷 646-659页

作者： Zhengyu An Aidi jiang Jingqi Chen Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina MOE Frontiers Center for Brain Science Fudan UniversityShanghaiChina MOE Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence Fudan UniversityShanghaiChina Zhangjiang Fudan International Innovation Center ShanghaiChina

Neurodegenerative diseases cause great medical and economic burdens for both patients and society;however, the complex molecular mechanisms thereof are not yet well understood. With the development of high-coverage sequencing technology, researchers have started to notice that genomic repeat regions, previously neglected in search of disease culprits, are active contributors to multiple neurodegenerative diseases. In this review, we describe the association between repeat element variants and multiple degenerative diseases through genome-wide association studies and targeted sequencing. We discuss the identification of disease-relevant repeat element variants, further powered by the advancement of long-read sequencing technologies and their related tools, and summarize recent findings in the molecular mechanisms of repeat element variants in brain degeneration, such as those causing transcriptional silencing or RNA-mediated gain of toxic function. Furthermore, we describe how in silico predictions using innovative computational models, such as deep learning language models, could enhance and accelerate our understanding of the functional impact of repeat element variants. Finally, we discuss future directions to advance current findings for a better understanding of neurodegenerative diseases and the clinical applications of genomic repeat elements.

关键词： Alzheimer's disease ataxia deep learning long-read sequencing neurodegeneration neurodegenerative diseases Parkinson's disease repeat element structural variant

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