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谷胱甘肽亲和层析介质的制备条件及其层析特性研究

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天津科技大学学报 2015年第5期30卷 7-14页

作者：易秋分张翠英蒋永吉唐昭娜陈文成朱显明刘俊伟董辉天津科技大学生物工程学院天津300457 天津国际生物医药联合研究院天津300457

以琼脂糖凝胶4FF（Sepharose 4 Fast Flow）为基质,1,4-丁二醇二缩水甘油醚（BDGE）为活化剂,通过活化、偶联谷胱甘肽（GSH）,得到GSH亲和层析介质.实验结果表明：活化及偶联的优化条件为10,m L基质加入0.42,g Na OH、15,m L二甲基亚砜... 详细信息

以琼脂糖凝胶4FF（Sepharose 4 Fast Flow）为基质,1,4-丁二醇二缩水甘油醚（BDGE）为活化剂,通过活化、偶联谷胱甘肽（GSH）,得到GSH亲和层析介质.实验结果表明：活化及偶联的优化条件为10,m L基质加入0.42,g Na OH、15,m L二甲基亚砜（DMSO）、15,m L BDGE,反应温度为40,℃,反应时间为6,h;偶联溶液p H 6.5,反应温度为37,℃,反应时间为24,h.在此条件下,环氧基修饰密度可达60~65,μmol/m L;所制备的介质载量为（14.19±0.98）mg/m L.以此介质对谷胱甘肽S–转移酶（GST）、融合蛋白GST-Bcl-2、GST-Ald、GST-PTPN12进行纯化,结果表明,该介质纯化效果较好、载量高、稳定性能较好,可重复使用.

关键词：谷胱甘肽亲和层析活化环氧基修饰密度载量

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基于压痕功的加工表面残余应力测试实验

上海航天（中英文） 2020年第3期37卷 115-120页

作者：邱硕杨文玉周金强陈文成孔维森上海航天设备制造总厂有限公司上海200245 华中科技大学数字制造装备与技术国家重点实验室湖北武汉430074

可调距螺旋桨的加工表面质量对其高服役性能和长寿命服役具有直接而重要的影响。本文开展加工表面残余应力测量和数据处理的研究,采用基于压痕功的残余应力测量方法及神经网络求解算法,针对镍铝青铜材料力学性能,由有限元仿真分析得到... 详细信息

可调距螺旋桨的加工表面质量对其高服役性能和长寿命服役具有直接而重要的影响。本文开展加工表面残余应力测量和数据处理的研究,采用基于压痕功的残余应力测量方法及神经网络求解算法,针对镍铝青铜材料力学性能,由有限元仿真分析得到关系数据库,训练神经网络,实现对镍铝青铜材料加工残余应力的求解。通过对镍铝青铜加工试件残余应力测量数据的计算与分析评价,开展了不同加工参数对残余应力影响规律的实验测量研究。通过测量实验与数据分析,获得了不同刀路下残留高度及机床不同转速与残余应力间的关系。测试实验表明:基于压痕功残余应力测试方法能评价不同加工参数下镍铝青铜材料的残余应力状态,可为实际加工生产提供工艺参数优化指导。

关键词：残余应力压痕功加工参数

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基于氟碳树脂的蓄能发光涂料的制备及性能

电镀与涂饰 2023年第4期42卷 43-49页

作者：唐念吴晓慧孙东伟周永言张曼君刘博陈文铖霍延平广东电网有限责任公司电力科学研究院广东广州510080 广东工业大学轻工化工学院广东广州510006

以氟碳−聚氨酯为成膜物质,与无机长余辉发光颜料(SrAl_(2)O_(4):Eu^(2+),Dy^(3+))及助剂共混制成了一种蓄能发光涂料。探讨了钛白粉的用量、发光颜料的筛选、单层与双层涂膜的发光性能等方面的影响因素。结果表明,所制备的双层涂膜有高... 详细信息

以氟碳−聚氨酯为成膜物质,与无机长余辉发光颜料(SrAl_(2)O_(4):Eu^(2+),Dy^(3+))及助剂共混制成了一种蓄能发光涂料。探讨了钛白粉的用量、发光颜料的筛选、单层与双层涂膜的发光性能等方面的影响因素。结果表明,所制备的双层涂膜有高达21.09 cd/m^(2)的初始亮度,余辉时间长达8 h,且能够满足电力设备涂料应用中对耐候性、耐热性、耐水性等的要求。

关键词：长余辉涂料氟碳树脂稀土掺杂铝酸锶发光颜料耐候性

Tranylcypromine upregulates Sestrin 2 expression to ameliorate NLRP3-related noise-induced hearing loss

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Neural Regeneration Research 2025年第5期20卷 1483-1494页

作者： Xihang chen Zhifeng chen Menghua Li Weiwei Guo Shuolong Yuan Liangwei Xu Chang Lin Xi Shi Wei chen Shiming Yang Senior Department of Otolaryngology Head and Neck Surgery Chinese PLA General HospitalChinese PLA Medical SchoolBeijingChina State Key Laboratory of Hearing and Balance Science BeijingChina National Clinical Research Center for Otolaryngologic Diseases BeijingChina Key Laboratory of Hearing Science Ministry of EducationBeijingChina Beijing Key Laboratory of Hearing Impairment Prevention and Treatment BeijingChina Department of Otorhinolaryngology Head and Neck Surgery The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Otolaryngology Head and Neck Surgery The 940th Hospital of Joint Logistics Support Force of Chinese PLALanzhouGansu ProvinceChina Key Laboratory of Tropical Biological Resources of Ministry of Education School of Pharmaceutical SciencesHainan UniversityHaikouHainan ProvinceChina Academician Workstation of Hainan University School of Pharmaceutical SciencesYazhou BaySanyaHainan ProvinceChina

Noise-induced hearing loss is the primary non-genetic factor contributing to auditory ***,there are currently no effective pharmacological interventions for patients with noise-induced hearing ***,we present evidence suggesting that the lysine-specific demethylase 1 inhibitor–tranylcypromine is an otoprotective agent that could be used to treat noise-induced hearing loss,and elucidate its underlying regulatory *** established a mouse model of permanent threshold shift hearing loss by exposing the mice to white broadband noise at a sound pressure level of 120 d B for 4 *** found that tranylcypromine treatment led to the upregulation of Sestrin2(SESN2)and activation of the autophagy markers light chain 3B and lysosome-associated membrane glycoprotein 1 in the cochleae of mice treated with *** noise exposure group treated with tranylcypromine showed significantly lower average auditory brainstem response hearing thresholds at click,4,8,and 16 k Hz frequencies compared with the noise exposure group treated with *** findings indicate that tranylcypromine treatment resulted in increased SESN2,light chain 3B,and lysosome-associated membrane glycoprotein 1 expression after noise exposure,leading to a reduction in levels of 4-hydroxynonenal and cleaved caspase-3,thereby reducing noise-induced hair cell ***,immunoblot analysis demonstrated that treatment with tranylcypromine upregulated SESN2 expression via the autophagy *** treatment also reduced the production of NOD-like receptor family pyrin domaincontaining 3(NLRP3)*** conclusion,our results showed that tranylcypromine treatment ameliorated cochlear inflammation by promoting the expression of SESN2,which induced autophagy,thereby restricting NLRP3-related inflammasome signaling,alleviating cochlear hair cell loss,and protecting hearing *** findings suggest that inhibiting lysine-specific demethylase 1 is a

关键词： 4-hydroxynonenal apoptosis autophagy cleaved caspase-3 inflammation NOD-like receptor family pyrin domain-containing 3(NLRP3) noise-induced hearing loss oxidative stress Sestrin2 tranylcypromine

基于GIS的德化—尤溪矿集区金成矿远景信息量法预测

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商丘师范学院学报 2018年第9期34卷 56-60页

作者：王巧黄顺佳魏春慧江启煜陈文成泉州师范学院资环学院福建泉州362000 福建省闽东南地质大队福建泉州362000

综合应用地质、物探、化探、遥感等数据,进行多源融合的成矿预测,是适应当前找矿难度越来越大的需要.基于GIS,构建德化-尤溪矿集区金矿成矿预测空间数据库,划分1000 m×1000 m网格单元,利用数据驱动建模,通过GIS空间分析,挖掘地物化遥... 详细信息

综合应用地质、物探、化探、遥感等数据,进行多源融合的成矿预测,是适应当前找矿难度越来越大的需要.基于GIS,构建德化-尤溪矿集区金矿成矿预测空间数据库,划分1000 m×1000 m网格单元,利用数据驱动建模,通过GIS空间分析,挖掘地物化遥感多元找矿信息.在此基础,筛选17个找矿变量,进行信息量法综合信息成矿远景预测,圈定靶区4处及若干零星的远景区段,并划分为A、B、C类,对进一步部署金矿找矿工作具有重要意义.

关键词： GIS空间分析金矿成矿远景预测信息量法德化-尤溪

Laparoscopic liver resection utilizing the ventral avascular area of the inferior vena cava:A retrospective cohort study

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World Journal of Gastroenterology 2025年第1期31卷 42-57页

作者： Kun Huang Zhu chen Heng Xiao Hai-Yang Hu Xing-Yu chen cheng-You Du Xiang Lan Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400000China Department of General Surgery Mianyang Hospital of Traditional Chinese MedicineMianyang 621000Sichuan ProvinceChina

BACKGROUND Laparoscopic liver resection(LLR)can be challenging due to the difficulty of establishing a retrohepatic tunnel under *** the third hepatic hilum before parenchymal transection often leads to significant liver mobilization,tumor compression,and bleeding from the short hepatic veins(SHVs).This study introduces a novel technique utilizing the ventral avascular area of the inferior vena cava(IVC),allowing SHVs to be addressed after parenchymal transection,thereby reducing surgical complexity and improving outcomes in in situ *** To introduce and evaluate a novel LLR technique using the ventral avascular area of the IVC and compare its short-term outcomes with conventional *** The clinical cohort data of patients with pathologically confirmed hepatocellular carcinoma or intrahepatic cholangiocarcinoma who underwent conventional LLR and novel LLR between July 2021 and July 2023 at the First Affiliated Hospital of Chongqing Medical University were retrospectively *** novel LLR,we initially separated the caudate lobe from the IVC using dissecting forceps along the ventral avascular area of the ***,we transected the parenchyma of the left and right caudate lobes from the caudal side to the cephalic side using the avascular area as a ***,we addressed the SHVs and finally dissected the root of the right hepatic vein or left hepatic *** short-term postoperative outcomes and oncological results of the two approaches were evaluated and *** A total of 256 patients were included,with 150(58.59%)undergoing conventional LLR and 106(41.41%)undergoing novel *** novel technique resulted in significantly larger tumor resections(6.47±2.96 cm vs 4.01±2.33 cm,P<0.001),shorter operative times(199.57±60.37 minutes vs 262.33±83.90 minutes,P<0.001),less intraoperative blood loss(206.92±37.09 mL vs 363.34±131.27 mL,P<0.001),and greater resection volume(345.11±31.40 mL vs 264.38±31.98 mL,P<0.001)comp

关键词： Laparoscopic liver resection Inferior vena cava Retrohepatic tunnel Short hepatic veins Complex liver tumors Intraoperative bleeding control

Traditional Chinese medicine in the treatment of Helicobacter pylorirelated gastritis:The mechanisms of signalling pathway regulations

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World Journal of Gastroenterology 2025年第3期31卷 11-28页

作者： Pei-Pei Zhang Liang Li Hao-Yu Qu Guang-Yu chen Meng-Zhou Xie Yan-Kun chen School of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina Hunan Engineering Technology Research Center for Medicinal and Functional Food Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina Key Laboratory of Traditional Chinese Medicine Heart and Lung Syndrome Differentiation and Medicated Diet and Dietotherapy Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina School of Informatics Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Precision Medicine Research and Development Center Zhuhai Institute of Advanced TechnologyChinese Academy of SciencesZhuhai 519000Guangdong ProvinceChina

Helicobacter pylori-associated gastritis(HPAG)is a common condition of the gastrointestinal ***,extensive and long-term antibiotic use has resulted in numerous adverse effects,including increased resistance,gastrointestinal dysfunction,and increased recurrence *** these concerns develop,traditional Chinese medicine(TCM)may have *** is based on the concept of completeness and aims to eliminate pathogens and strengthen the *** has the potential to prevent this condition while also boosting the rate of Helicobacter pylori *** review elaborates on the mechanism of TCM treatment for HPAG based on cellular signalling pathways,which reflects the flexibility of TCM in treating diseases and the advantages of multi-level,multipathway,and multi-target treatments for HPAG.

关键词： Helicobacter pylori Helicobacter pylori-associated gastritis Traditional Chinese medicine Signalling pathway Eliminate pathogens Strengthen the body

福建省县域人口-经济时空分异及影响因素研究

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黑河学院学报 2024年第5期15卷 44-48页

作者：黄耀裔孙境蔚陈文成赵益民泉州师范学院资源与环境科学学院福建泉州362000

根据人口、经济及其相关指标数据,运用人口地理集中度、经济地理集中度和人口-经济匹配指数,对福建省人口与经济之间耦合关系和空间差异进行研究,并对其影响因素进行分析。结果表明:人口地理集中度在阈值为1时呈现东—西两级分异;经济... 详细信息

根据人口、经济及其相关指标数据,运用人口地理集中度、经济地理集中度和人口-经济匹配指数,对福建省人口与经济之间耦合关系和空间差异进行研究,并对其影响因素进行分析。结果表明:人口地理集中度在阈值为1时呈现东—西两级分异;经济地理集中度在阈值为1时呈现闽东和闽东南集聚态势,将人口-经济匹配指数按3类划分,2010—2021年期间同类型县域逐渐呈现集聚效应,福建省县域人口-经济匹配度整体有趋好态势,但匹配程度仍有待提高,人口-经济稳定型逐渐形成一个“U”字形,与福建省“十四五”规划较为吻合,说明该划分有利于宏观决策。在今后的发展中,福建省仍要注重区域间人口与经济发展水平相协调。

关键词：福建省人口地理集中度经济地理集中度人口与经济匹配指数时空分异

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长期深静脉留置双腔导管血液透析的临床应用

肾脏病与透析肾移植杂志 2004年第3期13卷 231-234页

作者：叶朝阳付文成戎殳陈静马晓红张斌梅长林第二军医大学附属长征医院解放军肾病中心上海200003

目的 :探讨长期血液透析留置双腔导管的效果和退出原因 ,延长其使用期限。　方法 :随访血液透析患者留置导管的使用情况和并发症 ,记录导管使用终点。　结果 :①实施深静脉留置长期带涤纶环导管术 1 39例次 ,其中 1 0例次为再次置管 ... 详细信息

目的 :探讨长期血液透析留置双腔导管的效果和退出原因 ,延长其使用期限。　方法 :随访血液透析患者留置导管的使用情况和并发症 ,记录导管使用终点。　结果 :①实施深静脉留置长期带涤纶环导管术 1 39例次 ,其中 1 0例次为再次置管 ;经颈内静脉入路 1 0 4例次 (成功率 98% ) ,经颈外静脉切开插管 1 3例次 (成功率 81 % ) ,经锁骨下静脉 2 2例次 (成功率 95 % )。置管成功率 1 0 0 %。②导管使用期限 :平均 (1 4 5± 1 0 4 )个月 ,使用留置导管时间分别为 :>72个月 1例 ,>36个月 7例 ,≥ 2 4个月 1 8例 ,≥ 1 2个月 37例次 ,≥ 6个月 4 2例次 ,<6个月 30例次 (大部分在继续使用 ) ,其余失访。观察导管使用终点 6 5例次 ,约占 4 6 % ,导管退出时平均使用时间 3～ 4 6 (1 0 2± 8 3)个月。退出原因 :患者死亡 4 0例 ,其余为感染拔管、导管拔脱、血流不畅和肾移植。在导管使用终点 ,90 % (5 8/ 6 5 )的导管功能良好。③导管透析充分性 :1 1 4例患者透析后尿素下降率平均 70 % ,平均Kt/V达 1 5 5。与内瘘透析患者Kt/V =1 5 0±0 33(n =30 )比较无显著差别。④导管动静脉端反接对透析效果影响 ,比较 35例导管动静脉端正接和反接 ,血流量2 0 0～ 2 5 0ml/min ,单次透析正接Kt/V =1 5 5± 0 4 3。

关键词：深静脉留置双腔导管血液透析血液净化纤维蛋白